Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3302 and Psyr_3303 are separated by 132 nucleotides Psyr_3303 and Psyr_3304 are separated by 17 nucleotides
Psyr_3302: Psyr_3302 - mannose-6-phosphate isomerase, type 2 / mannose-1-phosphate guanylyltransferase (GDP), at 3,951,223 to 3,952,680
_3302
Psyr_3303: Psyr_3303 - Polysaccharide export protein, at 3,952,813 to 3,953,592
_3303
Psyr_3304: Psyr_3304 - Lipopolysaccharide biosynthesis, at 3,953,610 to 3,955,601
_3304
Position (kb)
3952
3953
3954 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3952.114 kb on + strand, within Psyr_3302 at 3952.114 kb on + strand, within Psyr_3302 at 3952.115 kb on - strand, within Psyr_3302 at 3952.115 kb on - strand, within Psyr_3302 at 3952.115 kb on - strand, within Psyr_3302 at 3952.156 kb on + strand, within Psyr_3302 at 3952.156 kb on + strand, within Psyr_3302 at 3952.156 kb on + strand, within Psyr_3302 at 3952.156 kb on + strand, within Psyr_3302 at 3952.156 kb on + strand, within Psyr_3302 at 3952.157 kb on - strand, within Psyr_3302 at 3952.157 kb on - strand, within Psyr_3302 at 3952.157 kb on - strand, within Psyr_3302 at 3952.348 kb on + strand, within Psyr_3302 at 3952.348 kb on + strand, within Psyr_3302 at 3952.348 kb on + strand, within Psyr_3302 at 3952.348 kb on + strand, within Psyr_3302 at 3952.349 kb on - strand, within Psyr_3302 at 3952.349 kb on - strand, within Psyr_3302 at 3952.394 kb on + strand, within Psyr_3302 at 3952.394 kb on + strand, within Psyr_3302 at 3952.395 kb on - strand, within Psyr_3302 at 3952.395 kb on - strand, within Psyr_3302 at 3952.607 kb on - strand at 3952.645 kb on + strand at 3952.645 kb on + strand at 3952.646 kb on - strand at 3952.764 kb on + strand at 3952.765 kb on - strand at 3952.768 kb on - strand at 3952.784 kb on + strand at 3952.784 kb on + strand at 3952.784 kb on + strand at 3952.785 kb on - strand at 3952.785 kb on - strand at 3952.785 kb on - strand at 3952.841 kb on + strand at 3952.842 kb on - strand at 3952.842 kb on - strand at 3952.863 kb on + strand at 3953.044 kb on + strand, within Psyr_3303 at 3953.045 kb on - strand, within Psyr_3303 at 3953.045 kb on - strand, within Psyr_3303 at 3953.095 kb on + strand, within Psyr_3303 at 3953.095 kb on + strand, within Psyr_3303 at 3953.095 kb on + strand, within Psyr_3303 at 3953.096 kb on - strand, within Psyr_3303 at 3953.096 kb on - strand, within Psyr_3303 at 3953.096 kb on - strand, within Psyr_3303 at 3953.096 kb on - strand, within Psyr_3303 at 3953.096 kb on - strand, within Psyr_3303 at 3953.096 kb on - strand, within Psyr_3303 at 3953.096 kb on - strand, within Psyr_3303 at 3953.096 kb on - strand, within Psyr_3303 at 3953.096 kb on - strand, within Psyr_3303 at 3953.096 kb on - strand, within Psyr_3303 at 3953.096 kb on - strand, within Psyr_3303 at 3953.374 kb on + strand at 3953.375 kb on - strand, within Psyr_3303 at 3953.375 kb on - strand, within Psyr_3303 at 3953.375 kb on - strand, within Psyr_3303 at 3953.383 kb on + strand, within Psyr_3303 at 3953.383 kb on + strand, within Psyr_3303 at 3953.383 kb on + strand, within Psyr_3303 at 3953.384 kb on - strand, within Psyr_3303 at 3953.384 kb on - strand, within Psyr_3303 at 3953.384 kb on - strand, within Psyr_3303 at 3953.518 kb on + strand at 3953.519 kb on - strand at 3953.519 kb on - strand at 3953.569 kb on + strand at 3953.570 kb on - strand at 3953.570 kb on - strand at 3953.590 kb on + strand at 3953.590 kb on + strand at 3953.590 kb on + strand at 3953.591 kb on - strand at 3953.737 kb on - strand at 3953.737 kb on - strand at 3953.907 kb on + strand, within Psyr_3304 at 3953.907 kb on + strand, within Psyr_3304 at 3953.908 kb on - strand, within Psyr_3304 at 3953.908 kb on - strand, within Psyr_3304 at 3954.186 kb on + strand, within Psyr_3304 at 3954.238 kb on - strand, within Psyr_3304 at 3954.279 kb on + strand, within Psyr_3304 at 3954.279 kb on + strand, within Psyr_3304 at 3954.279 kb on + strand, within Psyr_3304 at 3954.280 kb on - strand, within Psyr_3304 at 3954.300 kb on + strand, within Psyr_3304 at 3954.300 kb on + strand, within Psyr_3304 at 3954.301 kb on - strand, within Psyr_3304 at 3954.301 kb on - strand, within Psyr_3304 at 3954.301 kb on - strand, within Psyr_3304 at 3954.326 kb on + strand, within Psyr_3304 at 3954.326 kb on + strand, within Psyr_3304 at 3954.327 kb on - strand, within Psyr_3304 at 3954.327 kb on - strand, within Psyr_3304
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,952,114 + Psyr_3302 0.61 +3.9 3,952,114 + Psyr_3302 0.61 +1.7 3,952,115 - Psyr_3302 0.61 +0.9 3,952,115 - Psyr_3302 0.61 -0.5 3,952,115 - Psyr_3302 0.61 -0.9 3,952,156 + Psyr_3302 0.64 +2.5 3,952,156 + Psyr_3302 0.64 -0.3 3,952,156 + Psyr_3302 0.64 +1.7 3,952,156 + Psyr_3302 0.64 -0.3 3,952,156 + Psyr_3302 0.64 +2.5 3,952,157 - Psyr_3302 0.64 -0.4 3,952,157 - Psyr_3302 0.64 -0.8 3,952,157 - Psyr_3302 0.64 -0.5 3,952,348 + Psyr_3302 0.77 +2.1 3,952,348 + Psyr_3302 0.77 +1.6 3,952,348 + Psyr_3302 0.77 +4.0 3,952,348 + Psyr_3302 0.77 +1.7 3,952,349 - Psyr_3302 0.77 +4.1 3,952,349 - Psyr_3302 0.77 +0.1 3,952,394 + Psyr_3302 0.80 -0.5 3,952,394 + Psyr_3302 0.80 -0.3 3,952,395 - Psyr_3302 0.80 +3.4 3,952,395 - Psyr_3302 0.80 -2.9 3,952,607 - +2.6 3,952,645 + -1.8 3,952,645 + +1.3 3,952,646 - +3.7 3,952,764 + +3.4 3,952,765 - +1.2 3,952,768 - -2.8 3,952,784 + -0.3 3,952,784 + -1.9 3,952,784 + -0.5 3,952,785 - -0.8 3,952,785 - +0.3 3,952,785 - -2.7 3,952,841 + -0.0 3,952,842 - -2.0 3,952,842 - +1.6 3,952,863 + +0.1 3,953,044 + Psyr_3303 0.30 +1.7 3,953,045 - Psyr_3303 0.30 +0.4 3,953,045 - Psyr_3303 0.30 +0.1 3,953,095 + Psyr_3303 0.36 -0.4 3,953,095 + Psyr_3303 0.36 +2.9 3,953,095 + Psyr_3303 0.36 +3.3 3,953,096 - Psyr_3303 0.36 -1.0 3,953,096 - Psyr_3303 0.36 -1.7 3,953,096 - Psyr_3303 0.36 +2.6 3,953,096 - Psyr_3303 0.36 +0.2 3,953,096 - Psyr_3303 0.36 -0.3 3,953,096 - Psyr_3303 0.36 +2.3 3,953,096 - Psyr_3303 0.36 -1.1 3,953,096 - Psyr_3303 0.36 +3.8 3,953,096 - Psyr_3303 0.36 +0.7 3,953,096 - Psyr_3303 0.36 +2.6 3,953,096 - Psyr_3303 0.36 -0.0 3,953,374 + -4.6 3,953,375 - Psyr_3303 0.72 -2.6 3,953,375 - Psyr_3303 0.72 -1.3 3,953,375 - Psyr_3303 0.72 +0.6 3,953,383 + Psyr_3303 0.73 +0.8 3,953,383 + Psyr_3303 0.73 -2.3 3,953,383 + Psyr_3303 0.73 -1.1 3,953,384 - Psyr_3303 0.73 +0.6 3,953,384 - Psyr_3303 0.73 +3.3 3,953,384 - Psyr_3303 0.73 +2.6 3,953,518 + +1.7 3,953,519 - -1.7 3,953,519 - +2.1 3,953,569 + -1.0 3,953,570 - -0.9 3,953,570 - +1.1 3,953,590 + -1.6 3,953,590 + -3.3 3,953,590 + -0.3 3,953,591 - +1.3 3,953,737 - -3.6 3,953,737 - +2.6 3,953,907 + Psyr_3304 0.15 -0.5 3,953,907 + Psyr_3304 0.15 +3.7 3,953,908 - Psyr_3304 0.15 +2.6 3,953,908 - Psyr_3304 0.15 -0.3 3,954,186 + Psyr_3304 0.29 +2.7 3,954,238 - Psyr_3304 0.32 -3.3 3,954,279 + Psyr_3304 0.34 +3.9 3,954,279 + Psyr_3304 0.34 +2.6 3,954,279 + Psyr_3304 0.34 -0.6 3,954,280 - Psyr_3304 0.34 +0.7 3,954,300 + Psyr_3304 0.35 +0.7 3,954,300 + Psyr_3304 0.35 -0.4 3,954,301 - Psyr_3304 0.35 -2.2 3,954,301 - Psyr_3304 0.35 +2.7 3,954,301 - Psyr_3304 0.35 -3.3 3,954,326 + Psyr_3304 0.36 -1.1 3,954,326 + Psyr_3304 0.36 -1.1 3,954,327 - Psyr_3304 0.36 +1.7 3,954,327 - Psyr_3304 0.36 -1.6
Or see this region's nucleotide sequence