Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3279 and Psyr_3280 are separated by 1 nucleotides Psyr_3280 and Psyr_3281 are separated by 9 nucleotides Psyr_3281 and Psyr_3282 are separated by 125 nucleotides
Psyr_3279: Psyr_3279 - Cytochrome B561, bacterial, at 3,927,085 to 3,927,624
_3279
Psyr_3280: Psyr_3280 - methylthioadenosine phosphorylase, at 3,927,626 to 3,928,366
_3280
Psyr_3281: Psyr_3281 - Glycoside hydrolase, family 3, N-terminal, at 3,928,376 to 3,929,386
_3281
Psyr_3282: Psyr_3282 - transcriptional regulator, TetR family, at 3,929,512 to 3,930,228
_3282
Position (kb)
3928
3929
3930 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3927.398 kb on + strand, within Psyr_3279 at 3927.541 kb on + strand, within Psyr_3279 at 3927.541 kb on + strand, within Psyr_3279 at 3927.541 kb on + strand, within Psyr_3279 at 3927.541 kb on + strand, within Psyr_3279 at 3927.542 kb on - strand, within Psyr_3279 at 3927.542 kb on - strand, within Psyr_3279 at 3927.542 kb on - strand, within Psyr_3279 at 3927.542 kb on - strand, within Psyr_3279 at 3927.542 kb on - strand, within Psyr_3279 at 3927.542 kb on - strand, within Psyr_3279 at 3927.542 kb on - strand, within Psyr_3279 at 3927.544 kb on + strand, within Psyr_3279 at 3927.544 kb on + strand, within Psyr_3279 at 3927.544 kb on + strand, within Psyr_3279 at 3927.544 kb on + strand, within Psyr_3279 at 3927.544 kb on + strand, within Psyr_3279 at 3927.544 kb on + strand, within Psyr_3279 at 3927.544 kb on + strand, within Psyr_3279 at 3927.544 kb on + strand, within Psyr_3279 at 3927.545 kb on - strand, within Psyr_3279 at 3927.545 kb on - strand, within Psyr_3279 at 3927.545 kb on - strand, within Psyr_3279 at 3927.545 kb on - strand, within Psyr_3279 at 3927.545 kb on - strand, within Psyr_3279 at 3927.545 kb on - strand, within Psyr_3279 at 3927.545 kb on - strand, within Psyr_3279 at 3927.545 kb on - strand, within Psyr_3279 at 3927.545 kb on - strand, within Psyr_3279 at 3927.545 kb on - strand, within Psyr_3279 at 3927.622 kb on + strand at 3927.622 kb on + strand at 3927.623 kb on - strand at 3927.623 kb on - strand at 3927.623 kb on - strand at 3927.766 kb on - strand, within Psyr_3280 at 3927.895 kb on - strand, within Psyr_3280 at 3928.045 kb on - strand, within Psyr_3280 at 3928.160 kb on - strand, within Psyr_3280 at 3928.170 kb on + strand, within Psyr_3280 at 3928.279 kb on - strand, within Psyr_3280 at 3928.348 kb on + strand at 3928.480 kb on + strand, within Psyr_3281 at 3928.741 kb on - strand, within Psyr_3281 at 3928.909 kb on + strand, within Psyr_3281 at 3929.147 kb on + strand, within Psyr_3281 at 3929.147 kb on + strand, within Psyr_3281 at 3929.386 kb on + strand at 3929.398 kb on + strand at 3929.664 kb on + strand, within Psyr_3282 at 3929.810 kb on + strand, within Psyr_3282 at 3929.810 kb on + strand at 3929.810 kb on + strand, within Psyr_3282 at 3929.811 kb on - strand, within Psyr_3282 at 3929.831 kb on + strand, within Psyr_3282 at 3929.831 kb on + strand, within Psyr_3282 at 3929.831 kb on + strand, within Psyr_3282 at 3929.831 kb on + strand, within Psyr_3282 at 3929.831 kb on + strand, within Psyr_3282 at 3929.831 kb on + strand, within Psyr_3282 at 3929.832 kb on - strand, within Psyr_3282 at 3929.832 kb on - strand, within Psyr_3282 at 3929.832 kb on - strand, within Psyr_3282 at 3929.832 kb on - strand, within Psyr_3282 at 3929.832 kb on - strand, within Psyr_3282 at 3929.832 kb on - strand, within Psyr_3282 at 3929.832 kb on - strand, within Psyr_3282 at 3929.832 kb on - strand, within Psyr_3282 at 3929.832 kb on - strand, within Psyr_3282 at 3929.832 kb on - strand, within Psyr_3282 at 3929.832 kb on - strand, within Psyr_3282 at 3930.098 kb on + strand, within Psyr_3282 at 3930.138 kb on + strand, within Psyr_3282 at 3930.262 kb on + strand at 3930.262 kb on + strand at 3930.263 kb on - strand at 3930.263 kb on - strand at 3930.263 kb on - strand at 3930.263 kb on - strand at 3930.266 kb on + strand at 3930.334 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,927,398 + Psyr_3279 0.58 -1.8 3,927,541 + Psyr_3279 0.84 +3.6 3,927,541 + Psyr_3279 0.84 +0.2 3,927,541 + Psyr_3279 0.84 +3.2 3,927,541 + Psyr_3279 0.84 -3.2 3,927,542 - Psyr_3279 0.85 +2.3 3,927,542 - Psyr_3279 0.85 +1.3 3,927,542 - Psyr_3279 0.85 -0.6 3,927,542 - Psyr_3279 0.85 +1.1 3,927,542 - Psyr_3279 0.85 -0.3 3,927,542 - Psyr_3279 0.85 -0.3 3,927,542 - Psyr_3279 0.85 -2.9 3,927,544 + Psyr_3279 0.85 -1.1 3,927,544 + Psyr_3279 0.85 +0.7 3,927,544 + Psyr_3279 0.85 -3.1 3,927,544 + Psyr_3279 0.85 +3.7 3,927,544 + Psyr_3279 0.85 -1.3 3,927,544 + Psyr_3279 0.85 +2.1 3,927,544 + Psyr_3279 0.85 +3.1 3,927,544 + Psyr_3279 0.85 +2.9 3,927,545 - Psyr_3279 0.85 -0.5 3,927,545 - Psyr_3279 0.85 -0.3 3,927,545 - Psyr_3279 0.85 +2.6 3,927,545 - Psyr_3279 0.85 +3.1 3,927,545 - Psyr_3279 0.85 +2.7 3,927,545 - Psyr_3279 0.85 -0.2 3,927,545 - Psyr_3279 0.85 -0.2 3,927,545 - Psyr_3279 0.85 -2.7 3,927,545 - Psyr_3279 0.85 -0.6 3,927,545 - Psyr_3279 0.85 -0.3 3,927,622 + +1.3 3,927,622 + +2.9 3,927,623 - +0.6 3,927,623 - -1.1 3,927,623 - +0.7 3,927,766 - Psyr_3280 0.19 +3.1 3,927,895 - Psyr_3280 0.36 -2.1 3,928,045 - Psyr_3280 0.57 -1.4 3,928,160 - Psyr_3280 0.72 +3.9 3,928,170 + Psyr_3280 0.73 -1.4 3,928,279 - Psyr_3280 0.88 -2.3 3,928,348 + +1.7 3,928,480 + Psyr_3281 0.10 -0.3 3,928,741 - Psyr_3281 0.36 -1.1 3,928,909 + Psyr_3281 0.53 -2.2 3,929,147 + Psyr_3281 0.76 -2.7 3,929,147 + Psyr_3281 0.76 -2.7 3,929,386 + +3.1 3,929,398 + -2.5 3,929,664 + Psyr_3282 0.21 +1.3 3,929,810 + Psyr_3282 0.42 -0.3 3,929,810 + +0.7 3,929,810 + Psyr_3282 0.42 -0.3 3,929,811 - Psyr_3282 0.42 -2.1 3,929,831 + Psyr_3282 0.44 -3.0 3,929,831 + Psyr_3282 0.44 -0.3 3,929,831 + Psyr_3282 0.44 +2.6 3,929,831 + Psyr_3282 0.44 -2.3 3,929,831 + Psyr_3282 0.44 +2.1 3,929,831 + Psyr_3282 0.44 -0.3 3,929,832 - Psyr_3282 0.45 +1.3 3,929,832 - Psyr_3282 0.45 -2.3 3,929,832 - Psyr_3282 0.45 -1.0 3,929,832 - Psyr_3282 0.45 +1.3 3,929,832 - Psyr_3282 0.45 -1.5 3,929,832 - Psyr_3282 0.45 +1.3 3,929,832 - Psyr_3282 0.45 +2.1 3,929,832 - Psyr_3282 0.45 +3.1 3,929,832 - Psyr_3282 0.45 -0.6 3,929,832 - Psyr_3282 0.45 -1.3 3,929,832 - Psyr_3282 0.45 +2.6 3,930,098 + Psyr_3282 0.82 +2.3 3,930,138 + Psyr_3282 0.87 -0.3 3,930,262 + +0.1 3,930,262 + +2.6 3,930,263 - -2.2 3,930,263 - -2.6 3,930,263 - +3.2 3,930,263 - +0.7 3,930,266 + +2.6 3,930,334 - -1.7
Or see this region's nucleotide sequence