Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3197 and Psyr_3198 are separated by 10 nucleotides Psyr_3198 and Psyr_3199 are separated by 74 nucleotides Psyr_3199 and Psyr_3200 are separated by 2 nucleotides Psyr_3200 and Psyr_3201 overlap by 4 nucleotides
Psyr_3197: Psyr_3197 - NADH dehydrogenase subunit A, at 3,827,706 to 3,828,119
_3197
Psyr_3198: Psyr_3198 - NADH dehydrogenase subunit B, at 3,828,130 to 3,828,804
_3198
Psyr_3199: Psyr_3199 - NADH dehydrogenase subunit D / NADH dehydrogenase subunit C, at 3,828,879 to 3,830,660
_3199
Psyr_3200: Psyr_3200 - NADH dehydrogenase subunit E, at 3,830,663 to 3,831,160
_3200
Psyr_3201: Psyr_3201 - NADH dehydrogenase subunit F, at 3,831,157 to 3,832,515
_3201
Position (kb)
3828
3829
3830
3831 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 3828.045 kb on + strand, within Psyr_3197 at 3828.045 kb on + strand, within Psyr_3197 at 3828.136 kb on + strand at 3828.137 kb on - strand at 3828.241 kb on + strand, within Psyr_3198 at 3828.241 kb on + strand, within Psyr_3198 at 3828.241 kb on + strand, within Psyr_3198 at 3828.242 kb on - strand, within Psyr_3198 at 3828.334 kb on + strand, within Psyr_3198 at 3828.334 kb on + strand, within Psyr_3198 at 3828.482 kb on - strand, within Psyr_3198 at 3828.602 kb on - strand, within Psyr_3198 at 3828.766 kb on - strand at 3828.823 kb on + strand at 3828.878 kb on + strand at 3828.900 kb on + strand at 3828.900 kb on + strand at 3828.912 kb on + strand at 3828.912 kb on + strand at 3828.913 kb on - strand at 3829.158 kb on + strand, within Psyr_3199 at 3829.158 kb on + strand, within Psyr_3199 at 3829.231 kb on + strand, within Psyr_3199 at 3829.503 kb on + strand, within Psyr_3199 at 3829.504 kb on - strand, within Psyr_3199 at 3829.504 kb on - strand, within Psyr_3199 at 3829.720 kb on - strand, within Psyr_3199 at 3829.836 kb on + strand, within Psyr_3199 at 3829.837 kb on - strand, within Psyr_3199 at 3829.899 kb on + strand, within Psyr_3199 at 3830.041 kb on - strand, within Psyr_3199 at 3830.041 kb on - strand, within Psyr_3199 at 3830.496 kb on + strand at 3830.885 kb on + strand, within Psyr_3200 at 3831.157 kb on - strand at 3831.157 kb on - strand at 3831.568 kb on - strand, within Psyr_3201 at 3831.568 kb on - strand, within Psyr_3201
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,828,045 + Psyr_3197 0.82 +0.8 3,828,045 + Psyr_3197 0.82 -0.7 3,828,136 + -0.2 3,828,137 - -0.2 3,828,241 + Psyr_3198 0.16 +1.8 3,828,241 + Psyr_3198 0.16 +1.4 3,828,241 + Psyr_3198 0.16 +1.4 3,828,242 - Psyr_3198 0.17 -0.2 3,828,334 + Psyr_3198 0.30 +2.6 3,828,334 + Psyr_3198 0.30 -0.2 3,828,482 - Psyr_3198 0.52 +0.8 3,828,602 - Psyr_3198 0.70 -2.5 3,828,766 - +1.4 3,828,823 + +2.8 3,828,878 + +0.8 3,828,900 + -0.2 3,828,900 + -3.7 3,828,912 + +0.8 3,828,912 + -0.2 3,828,913 - -3.9 3,829,158 + Psyr_3199 0.16 -0.2 3,829,158 + Psyr_3199 0.16 -2.8 3,829,231 + Psyr_3199 0.20 -4.6 3,829,503 + Psyr_3199 0.35 +1.4 3,829,504 - Psyr_3199 0.35 -2.8 3,829,504 - Psyr_3199 0.35 -2.5 3,829,720 - Psyr_3199 0.47 +1.4 3,829,836 + Psyr_3199 0.54 -0.2 3,829,837 - Psyr_3199 0.54 +0.8 3,829,899 + Psyr_3199 0.57 +1.4 3,830,041 - Psyr_3199 0.65 -5.1 3,830,041 - Psyr_3199 0.65 +0.8 3,830,496 + -0.2 3,830,885 + Psyr_3200 0.45 -4.1 3,831,157 - -2.9 3,831,157 - +1.8 3,831,568 - Psyr_3201 0.30 +2.6 3,831,568 - Psyr_3201 0.30 +0.8
Or see this region's nucleotide sequence