Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3142 and Psyr_3143 overlap by 4 nucleotides Psyr_3143 and Psyr_3144 overlap by 26 nucleotides Psyr_3144 and Psyr_3145 overlap by 4 nucleotides Psyr_3145 and Psyr_3146 overlap by 4 nucleotides
Psyr_3142: Psyr_3142 - general secretion pathway protein N, putative, at 3,769,113 to 3,769,643
_3142
Psyr_3143: Psyr_3143 - general secretion pathway protein M, putative, at 3,769,640 to 3,770,251
_3143
Psyr_3144: Psyr_3144 - Fimbrial assembly, at 3,770,226 to 3,771,320
_3144
Psyr_3145: Psyr_3145 - general secretion pathway protein K, putative, at 3,771,317 to 3,772,051
_3145
Psyr_3146: Psyr_3146 - general secretion pathway protein J, putative, at 3,772,048 to 3,772,680
_3146
Position (kb)
3770
3771
3772 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3769.449 kb on - strand, within Psyr_3142 at 3769.629 kb on + strand at 3769.629 kb on + strand at 3769.629 kb on + strand at 3769.630 kb on - strand at 3769.630 kb on - strand at 3769.630 kb on - strand at 3769.630 kb on - strand at 3769.750 kb on + strand, within Psyr_3143 at 3769.750 kb on + strand, within Psyr_3143 at 3769.750 kb on + strand, within Psyr_3143 at 3769.750 kb on + strand, within Psyr_3143 at 3769.751 kb on - strand, within Psyr_3143 at 3770.176 kb on - strand, within Psyr_3143 at 3770.450 kb on + strand, within Psyr_3144 at 3770.542 kb on + strand, within Psyr_3144 at 3770.542 kb on + strand, within Psyr_3144 at 3770.543 kb on - strand, within Psyr_3144 at 3770.543 kb on - strand, within Psyr_3144 at 3770.543 kb on - strand, within Psyr_3144 at 3770.543 kb on - strand, within Psyr_3144 at 3770.543 kb on - strand, within Psyr_3144 at 3770.874 kb on + strand, within Psyr_3144 at 3770.959 kb on + strand, within Psyr_3144 at 3770.960 kb on - strand, within Psyr_3144 at 3770.960 kb on - strand, within Psyr_3144 at 3770.960 kb on - strand, within Psyr_3144 at 3771.342 kb on + strand at 3771.389 kb on + strand at 3771.440 kb on + strand, within Psyr_3145 at 3771.545 kb on + strand, within Psyr_3145 at 3771.551 kb on + strand, within Psyr_3145 at 3771.552 kb on - strand, within Psyr_3145 at 3771.552 kb on - strand, within Psyr_3145 at 3771.552 kb on - strand, within Psyr_3145 at 3771.552 kb on - strand, within Psyr_3145 at 3771.573 kb on + strand, within Psyr_3145 at 3771.573 kb on + strand, within Psyr_3145 at 3771.574 kb on - strand, within Psyr_3145 at 3771.574 kb on - strand, within Psyr_3145 at 3771.574 kb on - strand, within Psyr_3145 at 3771.574 kb on - strand, within Psyr_3145 at 3771.574 kb on - strand, within Psyr_3145 at 3771.615 kb on - strand, within Psyr_3145 at 3771.615 kb on - strand, within Psyr_3145 at 3771.719 kb on + strand, within Psyr_3145 at 3771.720 kb on - strand, within Psyr_3145 at 3771.720 kb on - strand, within Psyr_3145 at 3771.720 kb on - strand, within Psyr_3145 at 3771.720 kb on - strand, within Psyr_3145 at 3771.720 kb on - strand, within Psyr_3145 at 3771.721 kb on + strand, within Psyr_3145 at 3771.722 kb on - strand, within Psyr_3145 at 3771.800 kb on - strand, within Psyr_3145 at 3771.800 kb on - strand, within Psyr_3145 at 3771.866 kb on + strand, within Psyr_3145 at 3771.867 kb on - strand, within Psyr_3145 at 3771.911 kb on - strand, within Psyr_3145 at 3772.007 kb on + strand at 3772.008 kb on - strand at 3772.255 kb on + strand, within Psyr_3146 at 3772.256 kb on - strand, within Psyr_3146 at 3772.256 kb on - strand, within Psyr_3146 at 3772.256 kb on - strand, within Psyr_3146
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,769,449 - Psyr_3142 0.63 -2.2 3,769,629 + -1.5 3,769,629 + +3.1 3,769,629 + +3.2 3,769,630 - +2.8 3,769,630 - -1.7 3,769,630 - +3.1 3,769,630 - -0.4 3,769,750 + Psyr_3143 0.18 +3.1 3,769,750 + Psyr_3143 0.18 +4.5 3,769,750 + Psyr_3143 0.18 -1.0 3,769,750 + Psyr_3143 0.18 +0.1 3,769,751 - Psyr_3143 0.18 +2.1 3,770,176 - Psyr_3143 0.88 +2.9 3,770,450 + Psyr_3144 0.20 -1.2 3,770,542 + Psyr_3144 0.29 +3.6 3,770,542 + Psyr_3144 0.29 -3.0 3,770,543 - Psyr_3144 0.29 +0.1 3,770,543 - Psyr_3144 0.29 +3.2 3,770,543 - Psyr_3144 0.29 -0.2 3,770,543 - Psyr_3144 0.29 -0.9 3,770,543 - Psyr_3144 0.29 -1.9 3,770,874 + Psyr_3144 0.59 +1.8 3,770,959 + Psyr_3144 0.67 +0.8 3,770,960 - Psyr_3144 0.67 +1.8 3,770,960 - Psyr_3144 0.67 -0.3 3,770,960 - Psyr_3144 0.67 +0.3 3,771,342 + -4.5 3,771,389 + -1.1 3,771,440 + Psyr_3145 0.17 -1.8 3,771,545 + Psyr_3145 0.31 +0.8 3,771,551 + Psyr_3145 0.32 -1.8 3,771,552 - Psyr_3145 0.32 -0.3 3,771,552 - Psyr_3145 0.32 +1.8 3,771,552 - Psyr_3145 0.32 +2.3 3,771,552 - Psyr_3145 0.32 -1.2 3,771,573 + Psyr_3145 0.35 -2.2 3,771,573 + Psyr_3145 0.35 -1.6 3,771,574 - Psyr_3145 0.35 +4.0 3,771,574 - Psyr_3145 0.35 +4.0 3,771,574 - Psyr_3145 0.35 +3.5 3,771,574 - Psyr_3145 0.35 +2.9 3,771,574 - Psyr_3145 0.35 +0.2 3,771,615 - Psyr_3145 0.41 -2.5 3,771,615 - Psyr_3145 0.41 +1.3 3,771,719 + Psyr_3145 0.55 -1.1 3,771,720 - Psyr_3145 0.55 +2.8 3,771,720 - Psyr_3145 0.55 +2.6 3,771,720 - Psyr_3145 0.55 -0.2 3,771,720 - Psyr_3145 0.55 +2.1 3,771,720 - Psyr_3145 0.55 -1.4 3,771,721 + Psyr_3145 0.55 +2.8 3,771,722 - Psyr_3145 0.55 -3.6 3,771,800 - Psyr_3145 0.66 +4.0 3,771,800 - Psyr_3145 0.66 -2.0 3,771,866 + Psyr_3145 0.75 +1.2 3,771,867 - Psyr_3145 0.75 +1.1 3,771,911 - Psyr_3145 0.81 -1.3 3,772,007 + -0.2 3,772,008 - +0.7 3,772,255 + Psyr_3146 0.33 +3.9 3,772,256 - Psyr_3146 0.33 +3.1 3,772,256 - Psyr_3146 0.33 +3.3 3,772,256 - Psyr_3146 0.33 -2.0
Or see this region's nucleotide sequence