Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3106 and Psyr_3107 are separated by 518 nucleotides Psyr_3107 and Psyr_3108 are separated by 93 nucleotides Psyr_3108 and Psyr_3109 overlap by 25 nucleotides
Psyr_3106: Psyr_3106 - phosphate ABC transporter substrate-binding protein, PhoT family, at 3,723,649 to 3,724,668
_3106
Psyr_3107: Psyr_3107 - conserved hypothetical protein, at 3,725,187 to 3,725,471
_3107
Psyr_3108: Psyr_3108 - conserved hypothetical protein, at 3,725,565 to 3,725,867
_3108
Psyr_3109: Psyr_3109 - conserved hypothetical protein, at 3,725,843 to 3,727,546
_3109
Position (kb)
3725
3726 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3724.503 kb on - strand, within Psyr_3106 at 3724.532 kb on + strand, within Psyr_3106 at 3724.588 kb on + strand at 3724.589 kb on - strand at 3724.589 kb on - strand at 3724.589 kb on - strand at 3724.589 kb on - strand at 3724.674 kb on + strand at 3724.675 kb on - strand at 3724.675 kb on - strand at 3724.708 kb on + strand at 3724.708 kb on + strand at 3724.708 kb on + strand at 3724.708 kb on + strand at 3724.709 kb on - strand at 3724.709 kb on - strand at 3724.751 kb on + strand at 3724.863 kb on - strand at 3724.863 kb on - strand at 3724.863 kb on - strand at 3724.924 kb on - strand at 3724.980 kb on + strand at 3724.987 kb on - strand at 3724.987 kb on - strand at 3725.082 kb on - strand at 3725.102 kb on + strand at 3725.103 kb on - strand at 3725.132 kb on + strand at 3725.132 kb on + strand at 3725.133 kb on - strand at 3725.161 kb on + strand at 3725.162 kb on - strand at 3725.166 kb on + strand at 3725.166 kb on + strand at 3725.181 kb on - strand at 3725.236 kb on + strand, within Psyr_3107 at 3725.236 kb on + strand, within Psyr_3107 at 3725.236 kb on + strand, within Psyr_3107 at 3725.237 kb on - strand, within Psyr_3107 at 3725.237 kb on - strand, within Psyr_3107 at 3725.237 kb on - strand, within Psyr_3107 at 3725.483 kb on - strand at 3725.524 kb on - strand at 3725.546 kb on + strand at 3725.547 kb on - strand at 3725.550 kb on + strand at 3725.576 kb on + strand at 3725.576 kb on + strand at 3725.577 kb on - strand at 3725.577 kb on - strand at 3725.577 kb on - strand at 3725.590 kb on + strand at 3725.591 kb on - strand at 3725.591 kb on - strand at 3725.591 kb on - strand at 3725.922 kb on + strand at 3725.922 kb on + strand at 3725.923 kb on - strand at 3725.923 kb on - strand at 3726.083 kb on + strand, within Psyr_3109 at 3726.083 kb on + strand, within Psyr_3109 at 3726.083 kb on + strand, within Psyr_3109 at 3726.083 kb on + strand, within Psyr_3109 at 3726.084 kb on - strand, within Psyr_3109 at 3726.084 kb on - strand, within Psyr_3109 at 3726.084 kb on - strand, within Psyr_3109 at 3726.084 kb on - strand, within Psyr_3109 at 3726.183 kb on - strand, within Psyr_3109 at 3726.205 kb on - strand, within Psyr_3109 at 3726.240 kb on + strand, within Psyr_3109 at 3726.241 kb on - strand, within Psyr_3109 at 3726.279 kb on - strand, within Psyr_3109 at 3726.297 kb on + strand, within Psyr_3109 at 3726.297 kb on + strand, within Psyr_3109 at 3726.297 kb on + strand, within Psyr_3109 at 3726.298 kb on - strand, within Psyr_3109 at 3726.298 kb on - strand, within Psyr_3109 at 3726.298 kb on - strand, within Psyr_3109 at 3726.298 kb on - strand, within Psyr_3109 at 3726.298 kb on - strand, within Psyr_3109 at 3726.426 kb on + strand, within Psyr_3109 at 3726.426 kb on + strand, within Psyr_3109 at 3726.427 kb on - strand, within Psyr_3109 at 3726.427 kb on - strand, within Psyr_3109 at 3726.427 kb on - strand, within Psyr_3109 at 3726.427 kb on - strand, within Psyr_3109 at 3726.468 kb on - strand, within Psyr_3109
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,724,503 - Psyr_3106 0.84 -1.6 3,724,532 + Psyr_3106 0.87 +2.9 3,724,588 + -3.0 3,724,589 - +1.8 3,724,589 - +1.3 3,724,589 - +0.8 3,724,589 - +2.6 3,724,674 + +0.3 3,724,675 - +1.8 3,724,675 - +3.3 3,724,708 + -2.2 3,724,708 + -1.4 3,724,708 + +0.4 3,724,708 + -1.3 3,724,709 - +2.3 3,724,709 - +1.5 3,724,751 + +3.8 3,724,863 - -2.1 3,724,863 - +3.3 3,724,863 - +2.8 3,724,924 - +2.1 3,724,980 + -0.6 3,724,987 - +2.3 3,724,987 - +0.7 3,725,082 - -3.9 3,725,102 + +3.6 3,725,103 - -0.2 3,725,132 + -2.0 3,725,132 + -2.0 3,725,133 - -0.3 3,725,161 + +3.3 3,725,162 - +0.8 3,725,166 + +0.1 3,725,166 + +2.6 3,725,181 - +1.3 3,725,236 + Psyr_3107 0.17 +0.8 3,725,236 + Psyr_3107 0.17 -1.1 3,725,236 + Psyr_3107 0.17 -1.8 3,725,237 - Psyr_3107 0.18 -1.2 3,725,237 - Psyr_3107 0.18 -0.7 3,725,237 - Psyr_3107 0.18 -2.3 3,725,483 - +3.8 3,725,524 - +2.8 3,725,546 + -2.0 3,725,547 - -0.2 3,725,550 + +0.3 3,725,576 + +3.8 3,725,576 + +0.3 3,725,577 - +0.8 3,725,577 - -0.1 3,725,577 - -0.2 3,725,590 + +2.3 3,725,591 - +1.8 3,725,591 - +2.6 3,725,591 - +1.8 3,725,922 + -0.1 3,725,922 + -0.2 3,725,923 - -1.5 3,725,923 - +3.5 3,726,083 + Psyr_3109 0.14 -1.7 3,726,083 + Psyr_3109 0.14 +1.5 3,726,083 + Psyr_3109 0.14 +4.6 3,726,083 + Psyr_3109 0.14 +3.7 3,726,084 - Psyr_3109 0.14 +2.6 3,726,084 - Psyr_3109 0.14 -1.3 3,726,084 - Psyr_3109 0.14 +1.2 3,726,084 - Psyr_3109 0.14 -2.1 3,726,183 - Psyr_3109 0.20 +0.2 3,726,205 - Psyr_3109 0.21 -0.1 3,726,240 + Psyr_3109 0.23 -1.0 3,726,241 - Psyr_3109 0.23 +1.8 3,726,279 - Psyr_3109 0.26 -0.8 3,726,297 + Psyr_3109 0.27 -3.3 3,726,297 + Psyr_3109 0.27 -0.1 3,726,297 + Psyr_3109 0.27 -0.1 3,726,298 - Psyr_3109 0.27 -1.0 3,726,298 - Psyr_3109 0.27 -0.2 3,726,298 - Psyr_3109 0.27 -0.9 3,726,298 - Psyr_3109 0.27 +1.8 3,726,298 - Psyr_3109 0.27 +2.3 3,726,426 + Psyr_3109 0.34 +3.1 3,726,426 + Psyr_3109 0.34 -1.8 3,726,427 - Psyr_3109 0.34 -2.2 3,726,427 - Psyr_3109 0.34 -0.2 3,726,427 - Psyr_3109 0.34 -1.4 3,726,427 - Psyr_3109 0.34 +3.5 3,726,468 - Psyr_3109 0.37 +3.1
Or see this region's nucleotide sequence