Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3103 and Psyr_3104 overlap by 1 nucleotides Psyr_3104 and Psyr_3105 overlap by 4 nucleotides Psyr_3105 and Psyr_3106 are separated by 81 nucleotides
Psyr_3103: Psyr_3103 - phosphate ABC transporter ATP-binding protein, PhoT family, at 3,720,912 to 3,721,691
_3103
Psyr_3104: Psyr_3104 - phosphate ABC transporter membrane protein 2, PhoT family, at 3,721,691 to 3,722,575
_3104
Psyr_3105: Psyr_3105 - phosphate ABC transporter membrane protein 1, PhoT family, at 3,722,572 to 3,723,567
_3105
Psyr_3106: Psyr_3106 - phosphate ABC transporter substrate-binding protein, PhoT family, at 3,723,649 to 3,724,668
_3106
Position (kb)
3722
3723
3724 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 3721.718 kb on + strand at 3721.745 kb on + strand at 3721.746 kb on - strand at 3721.941 kb on - strand, within Psyr_3104 at 3722.181 kb on + strand, within Psyr_3104 at 3722.181 kb on + strand, within Psyr_3104 at 3722.263 kb on - strand, within Psyr_3104 at 3722.504 kb on + strand at 3722.569 kb on + strand at 3722.576 kb on - strand at 3722.693 kb on + strand, within Psyr_3105 at 3722.693 kb on + strand, within Psyr_3105 at 3722.862 kb on - strand, within Psyr_3105 at 3722.913 kb on - strand, within Psyr_3105 at 3723.146 kb on + strand, within Psyr_3105 at 3723.146 kb on + strand, within Psyr_3105 at 3723.147 kb on - strand, within Psyr_3105 at 3723.147 kb on - strand, within Psyr_3105 at 3723.417 kb on + strand, within Psyr_3105 at 3723.417 kb on + strand, within Psyr_3105 at 3723.417 kb on + strand, within Psyr_3105 at 3723.417 kb on + strand, within Psyr_3105 at 3723.418 kb on - strand, within Psyr_3105 at 3723.418 kb on - strand, within Psyr_3105 at 3723.418 kb on - strand, within Psyr_3105 at 3723.548 kb on + strand at 3723.650 kb on + strand at 3723.650 kb on + strand at 3723.650 kb on + strand at 3723.651 kb on - strand at 3723.651 kb on - strand at 3723.651 kb on - strand at 3723.651 kb on - strand at 3723.651 kb on - strand at 3723.712 kb on + strand at 3723.713 kb on - strand at 3723.713 kb on - strand at 3723.713 kb on - strand at 3723.713 kb on - strand at 3723.713 kb on - strand at 3723.713 kb on - strand at 3723.713 kb on - strand at 3723.713 kb on - strand at 3723.713 kb on - strand at 3723.716 kb on + strand at 3723.716 kb on + strand at 3723.717 kb on - strand at 3723.717 kb on - strand at 3723.812 kb on + strand, within Psyr_3106 at 3723.813 kb on - strand, within Psyr_3106 at 3723.813 kb on - strand, within Psyr_3106 at 3723.813 kb on - strand, within Psyr_3106 at 3724.014 kb on - strand, within Psyr_3106 at 3724.052 kb on + strand, within Psyr_3106 at 3724.052 kb on + strand, within Psyr_3106 at 3724.053 kb on - strand, within Psyr_3106 at 3724.102 kb on + strand, within Psyr_3106 at 3724.157 kb on + strand, within Psyr_3106 at 3724.157 kb on + strand, within Psyr_3106 at 3724.158 kb on - strand, within Psyr_3106 at 3724.158 kb on - strand, within Psyr_3106 at 3724.158 kb on - strand, within Psyr_3106 at 3724.172 kb on + strand, within Psyr_3106 at 3724.172 kb on + strand, within Psyr_3106 at 3724.172 kb on + strand, within Psyr_3106 at 3724.173 kb on - strand, within Psyr_3106 at 3724.173 kb on - strand, within Psyr_3106 at 3724.173 kb on - strand, within Psyr_3106 at 3724.173 kb on - strand, within Psyr_3106 at 3724.173 kb on - strand, within Psyr_3106 at 3724.173 kb on - strand, within Psyr_3106 at 3724.503 kb on - strand, within Psyr_3106 at 3724.532 kb on + strand, within Psyr_3106
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,721,718 + +3.5 3,721,745 + -1.9 3,721,746 - +2.8 3,721,941 - Psyr_3104 0.28 -4.7 3,722,181 + Psyr_3104 0.55 +3.1 3,722,181 + Psyr_3104 0.55 +2.1 3,722,263 - Psyr_3104 0.65 -2.1 3,722,504 + -0.2 3,722,569 + -0.8 3,722,576 - -2.8 3,722,693 + Psyr_3105 0.12 -1.6 3,722,693 + Psyr_3105 0.12 +1.8 3,722,862 - Psyr_3105 0.29 +0.8 3,722,913 - Psyr_3105 0.34 -1.0 3,723,146 + Psyr_3105 0.58 +2.1 3,723,146 + Psyr_3105 0.58 -3.0 3,723,147 - Psyr_3105 0.58 -1.1 3,723,147 - Psyr_3105 0.58 -2.2 3,723,417 + Psyr_3105 0.85 +2.1 3,723,417 + Psyr_3105 0.85 -3.4 3,723,417 + Psyr_3105 0.85 -3.3 3,723,417 + Psyr_3105 0.85 +2.3 3,723,418 - Psyr_3105 0.85 +2.8 3,723,418 - Psyr_3105 0.85 -0.8 3,723,418 - Psyr_3105 0.85 +0.7 3,723,548 + +0.8 3,723,650 + -1.5 3,723,650 + -3.2 3,723,650 + -4.8 3,723,651 - -0.8 3,723,651 - -2.3 3,723,651 - +1.3 3,723,651 - +2.1 3,723,651 - +3.3 3,723,712 + -0.2 3,723,713 - -1.4 3,723,713 - +2.1 3,723,713 - +3.6 3,723,713 - -1.9 3,723,713 - +3.5 3,723,713 - +3.9 3,723,713 - -1.5 3,723,713 - +3.2 3,723,713 - -1.1 3,723,716 + +2.3 3,723,716 + -1.1 3,723,717 - +2.1 3,723,717 - -2.1 3,723,812 + Psyr_3106 0.16 +3.2 3,723,813 - Psyr_3106 0.16 +1.8 3,723,813 - Psyr_3106 0.16 -0.5 3,723,813 - Psyr_3106 0.16 +3.8 3,724,014 - Psyr_3106 0.36 +2.6 3,724,052 + Psyr_3106 0.40 +2.8 3,724,052 + Psyr_3106 0.40 -0.1 3,724,053 - Psyr_3106 0.40 +3.1 3,724,102 + Psyr_3106 0.44 +3.6 3,724,157 + Psyr_3106 0.50 -1.1 3,724,157 + Psyr_3106 0.50 -1.5 3,724,158 - Psyr_3106 0.50 +3.6 3,724,158 - Psyr_3106 0.50 +3.3 3,724,158 - Psyr_3106 0.50 +0.8 3,724,172 + Psyr_3106 0.51 -0.2 3,724,172 + Psyr_3106 0.51 +3.6 3,724,172 + Psyr_3106 0.51 +1.8 3,724,173 - Psyr_3106 0.51 +1.8 3,724,173 - Psyr_3106 0.51 +2.3 3,724,173 - Psyr_3106 0.51 +2.9 3,724,173 - Psyr_3106 0.51 -0.8 3,724,173 - Psyr_3106 0.51 +1.3 3,724,173 - Psyr_3106 0.51 +2.6 3,724,503 - Psyr_3106 0.84 -1.6 3,724,532 + Psyr_3106 0.87 +2.9
Or see this region's nucleotide sequence