Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3094 and Psyr_3095 overlap by 4 nucleotides Psyr_3095 and Psyr_3096 overlap by 4 nucleotides Psyr_3096 and Psyr_3097 are separated by 106 nucleotides
Psyr_3094: Psyr_3094 - Periplasmic binding protein, at 3,709,635 to 3,710,648
_3094
Psyr_3095: Psyr_3095 - transport system permease protein, at 3,710,645 to 3,711,667
_3095
Psyr_3096: Psyr_3096 - ABC transporter, at 3,711,664 to 3,712,455
_3096
Psyr_3097: Psyr_3097 - Peptidase U32, at 3,712,562 to 3,714,568
_3097
Position (kb)
3710
3711
3712 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3709.861 kb on + strand, within Psyr_3094 at 3709.861 kb on + strand, within Psyr_3094 at 3709.861 kb on + strand, within Psyr_3094 at 3709.861 kb on + strand, within Psyr_3094 at 3709.862 kb on - strand, within Psyr_3094 at 3710.040 kb on + strand, within Psyr_3094 at 3710.040 kb on + strand, within Psyr_3094 at 3710.040 kb on + strand, within Psyr_3094 at 3710.041 kb on - strand, within Psyr_3094 at 3710.041 kb on - strand, within Psyr_3094 at 3710.041 kb on - strand, within Psyr_3094 at 3710.041 kb on - strand, within Psyr_3094 at 3710.041 kb on - strand, within Psyr_3094 at 3710.041 kb on - strand, within Psyr_3094 at 3710.041 kb on - strand, within Psyr_3094 at 3710.041 kb on - strand, within Psyr_3094 at 3710.041 kb on - strand, within Psyr_3094 at 3710.127 kb on + strand, within Psyr_3094 at 3710.127 kb on + strand, within Psyr_3094 at 3710.127 kb on + strand, within Psyr_3094 at 3710.127 kb on + strand, within Psyr_3094 at 3710.127 kb on + strand, within Psyr_3094 at 3710.128 kb on - strand, within Psyr_3094 at 3710.128 kb on - strand, within Psyr_3094 at 3710.321 kb on + strand, within Psyr_3094 at 3710.321 kb on + strand, within Psyr_3094 at 3710.360 kb on + strand, within Psyr_3094 at 3710.361 kb on - strand, within Psyr_3094 at 3710.475 kb on + strand, within Psyr_3094 at 3710.475 kb on + strand, within Psyr_3094 at 3710.476 kb on - strand, within Psyr_3094 at 3710.792 kb on + strand, within Psyr_3095 at 3710.792 kb on + strand, within Psyr_3095 at 3710.792 kb on + strand, within Psyr_3095 at 3710.792 kb on + strand, within Psyr_3095 at 3710.792 kb on + strand, within Psyr_3095 at 3710.792 kb on + strand, within Psyr_3095 at 3710.792 kb on + strand, within Psyr_3095 at 3710.793 kb on - strand, within Psyr_3095 at 3710.793 kb on - strand, within Psyr_3095 at 3710.793 kb on - strand, within Psyr_3095 at 3710.807 kb on - strand, within Psyr_3095 at 3711.381 kb on + strand, within Psyr_3095 at 3711.382 kb on - strand, within Psyr_3095 at 3712.524 kb on + strand at 3712.655 kb on + strand at 3712.655 kb on + strand at 3712.655 kb on + strand at 3712.656 kb on - strand at 3712.656 kb on - strand at 3712.656 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,709,861 + Psyr_3094 0.22 +1.3 3,709,861 + Psyr_3094 0.22 +2.3 3,709,861 + Psyr_3094 0.22 -0.2 3,709,861 + Psyr_3094 0.22 +1.8 3,709,862 - Psyr_3094 0.22 -1.2 3,710,040 + Psyr_3094 0.40 +0.8 3,710,040 + Psyr_3094 0.40 -2.7 3,710,040 + Psyr_3094 0.40 -2.8 3,710,041 - Psyr_3094 0.40 -0.2 3,710,041 - Psyr_3094 0.40 -0.5 3,710,041 - Psyr_3094 0.40 -0.2 3,710,041 - Psyr_3094 0.40 -0.8 3,710,041 - Psyr_3094 0.40 +1.3 3,710,041 - Psyr_3094 0.40 -0.7 3,710,041 - Psyr_3094 0.40 +2.1 3,710,041 - Psyr_3094 0.40 +1.3 3,710,041 - Psyr_3094 0.40 -1.4 3,710,127 + Psyr_3094 0.49 -0.8 3,710,127 + Psyr_3094 0.49 +3.3 3,710,127 + Psyr_3094 0.49 +2.3 3,710,127 + Psyr_3094 0.49 -1.8 3,710,127 + Psyr_3094 0.49 -1.6 3,710,128 - Psyr_3094 0.49 -0.4 3,710,128 - Psyr_3094 0.49 -0.7 3,710,321 + Psyr_3094 0.68 -2.1 3,710,321 + Psyr_3094 0.68 +1.3 3,710,360 + Psyr_3094 0.71 +2.6 3,710,361 - Psyr_3094 0.72 +2.6 3,710,475 + Psyr_3094 0.83 -0.4 3,710,475 + Psyr_3094 0.83 -2.8 3,710,476 - Psyr_3094 0.83 +3.3 3,710,792 + Psyr_3095 0.14 +2.9 3,710,792 + Psyr_3095 0.14 -1.7 3,710,792 + Psyr_3095 0.14 +0.8 3,710,792 + Psyr_3095 0.14 +2.3 3,710,792 + Psyr_3095 0.14 -1.1 3,710,792 + Psyr_3095 0.14 +3.8 3,710,792 + Psyr_3095 0.14 +3.2 3,710,793 - Psyr_3095 0.14 +1.3 3,710,793 - Psyr_3095 0.14 +0.8 3,710,793 - Psyr_3095 0.14 +2.1 3,710,807 - Psyr_3095 0.16 -1.4 3,711,381 + Psyr_3095 0.72 -1.6 3,711,382 - Psyr_3095 0.72 +0.8 3,712,524 + +1.3 3,712,655 + +0.9 3,712,655 + -4.2 3,712,655 + +2.3 3,712,656 - +2.6 3,712,656 - -2.4 3,712,656 - -1.4
Or see this region's nucleotide sequence