Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3077 and Psyr_3078 are separated by 469 nucleotides Psyr_3078 and Psyr_3079 are separated by 73 nucleotides Psyr_3079 and Psyr_3080 are separated by 15 nucleotides
Psyr_3077: Psyr_3077 - Type I secretion outer membrane protein, TolC, at 3,680,341 to 3,681,816
_3077
Psyr_3078: Psyr_3078 - Peptidase M20:Peptidase M20, at 3,682,286 to 3,683,527
_3078
Psyr_3079: Psyr_3079 - CDS, at 3,683,601 to 3,684,083
_3079
Psyr_3080: Psyr_3080 - CDS, at 3,684,099 to 3,686,681
_3080
Position (kb)
3682
3683
3684 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3681.311 kb on + strand, within Psyr_3077 at 3681.505 kb on + strand, within Psyr_3077 at 3681.584 kb on + strand, within Psyr_3077 at 3681.634 kb on - strand, within Psyr_3077 at 3681.634 kb on - strand, within Psyr_3077 at 3681.635 kb on + strand, within Psyr_3077 at 3681.635 kb on + strand, within Psyr_3077 at 3681.635 kb on + strand, within Psyr_3077 at 3681.635 kb on + strand, within Psyr_3077 at 3681.636 kb on - strand, within Psyr_3077 at 3681.636 kb on - strand, within Psyr_3077 at 3681.636 kb on - strand, within Psyr_3077 at 3681.643 kb on + strand, within Psyr_3077 at 3681.643 kb on + strand, within Psyr_3077 at 3681.665 kb on - strand, within Psyr_3077 at 3681.791 kb on + strand at 3681.816 kb on + strand at 3681.817 kb on - strand at 3681.841 kb on - strand at 3681.843 kb on + strand at 3681.843 kb on + strand at 3681.844 kb on - strand at 3681.854 kb on + strand at 3681.869 kb on + strand at 3681.870 kb on - strand at 3681.886 kb on + strand at 3681.937 kb on + strand at 3681.963 kb on + strand at 3681.964 kb on - strand at 3681.964 kb on - strand at 3681.964 kb on - strand at 3681.966 kb on + strand at 3681.966 kb on + strand at 3681.997 kb on + strand at 3681.997 kb on + strand at 3681.998 kb on - strand at 3681.998 kb on - strand at 3681.998 kb on - strand at 3682.011 kb on + strand at 3682.015 kb on + strand at 3682.016 kb on - strand at 3682.016 kb on - strand at 3682.019 kb on + strand at 3682.019 kb on + strand at 3682.020 kb on - strand at 3682.020 kb on - strand at 3682.021 kb on + strand at 3682.022 kb on - strand at 3682.083 kb on + strand at 3682.084 kb on - strand at 3682.086 kb on + strand at 3682.086 kb on + strand at 3682.086 kb on + strand at 3682.181 kb on - strand at 3682.221 kb on + strand at 3682.221 kb on + strand at 3682.230 kb on - strand at 3682.249 kb on + strand at 3682.250 kb on - strand at 3682.250 kb on - strand at 3682.329 kb on + strand at 3682.329 kb on + strand at 3682.329 kb on + strand at 3682.329 kb on + strand at 3682.329 kb on + strand at 3682.330 kb on - strand at 3682.362 kb on + strand at 3682.362 kb on + strand at 3682.363 kb on - strand at 3682.363 kb on - strand at 3682.363 kb on - strand at 3682.363 kb on - strand at 3682.375 kb on + strand at 3682.498 kb on - strand, within Psyr_3078 at 3682.498 kb on - strand, within Psyr_3078 at 3682.498 kb on - strand, within Psyr_3078 at 3682.589 kb on - strand, within Psyr_3078 at 3682.813 kb on + strand, within Psyr_3078 at 3682.814 kb on - strand, within Psyr_3078 at 3683.073 kb on + strand, within Psyr_3078 at 3683.073 kb on + strand, within Psyr_3078 at 3683.073 kb on + strand, within Psyr_3078 at 3683.073 kb on + strand, within Psyr_3078 at 3683.073 kb on + strand, within Psyr_3078 at 3683.073 kb on + strand, within Psyr_3078 at 3683.074 kb on - strand, within Psyr_3078 at 3683.074 kb on - strand, within Psyr_3078 at 3683.074 kb on - strand, within Psyr_3078 at 3683.074 kb on - strand, within Psyr_3078 at 3683.140 kb on + strand, within Psyr_3078 at 3683.342 kb on + strand, within Psyr_3078 at 3683.343 kb on - strand, within Psyr_3078 at 3683.346 kb on + strand, within Psyr_3078 at 3683.406 kb on + strand at 3683.406 kb on + strand at 3683.406 kb on + strand at 3683.406 kb on + strand at 3683.407 kb on - strand at 3683.407 kb on - strand at 3683.407 kb on - strand at 3683.407 kb on - strand at 3683.407 kb on - strand at 3683.572 kb on + strand at 3683.572 kb on + strand at 3683.572 kb on + strand at 3683.572 kb on + strand at 3683.573 kb on - strand at 3683.573 kb on - strand at 3683.573 kb on - strand at 3684.085 kb on + strand at 3684.085 kb on + strand at 3684.085 kb on + strand at 3684.085 kb on + strand at 3684.086 kb on - strand at 3684.086 kb on - strand at 3684.086 kb on - strand at 3684.086 kb on - strand at 3684.101 kb on - strand at 3684.101 kb on - strand at 3684.123 kb on + strand at 3684.123 kb on + strand at 3684.124 kb on - strand at 3684.124 kb on - strand at 3684.149 kb on - strand at 3684.257 kb on + strand at 3684.518 kb on - strand, within Psyr_3080
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,681,311 + Psyr_3077 0.66 -0.0 3,681,505 + Psyr_3077 0.79 -0.3 3,681,584 + Psyr_3077 0.84 +2.1 3,681,634 - Psyr_3077 0.88 +1.4 3,681,634 - Psyr_3077 0.88 +1.8 3,681,635 + Psyr_3077 0.88 -2.7 3,681,635 + Psyr_3077 0.88 +2.6 3,681,635 + Psyr_3077 0.88 +0.2 3,681,635 + Psyr_3077 0.88 -0.9 3,681,636 - Psyr_3077 0.88 +4.0 3,681,636 - Psyr_3077 0.88 +1.4 3,681,636 - Psyr_3077 0.88 -1.3 3,681,643 + Psyr_3077 0.88 -1.2 3,681,643 + Psyr_3077 0.88 -2.1 3,681,665 - Psyr_3077 0.90 -0.5 3,681,791 + -3.2 3,681,816 + -0.8 3,681,817 - -0.3 3,681,841 - -0.2 3,681,843 + -1.0 3,681,843 + -3.3 3,681,844 - +1.8 3,681,854 + +0.2 3,681,869 + -1.5 3,681,870 - +2.4 3,681,886 + -1.6 3,681,937 + -2.0 3,681,963 + +1.7 3,681,964 - +1.4 3,681,964 - -0.1 3,681,964 - -0.3 3,681,966 + -1.9 3,681,966 + +2.4 3,681,997 + +4.0 3,681,997 + +3.0 3,681,998 - +0.8 3,681,998 - -0.5 3,681,998 - -1.7 3,682,011 + -1.3 3,682,015 + +1.4 3,682,016 - -2.2 3,682,016 - -1.4 3,682,019 + +0.2 3,682,019 + -0.9 3,682,020 - -2.9 3,682,020 - -3.2 3,682,021 + +1.8 3,682,022 - -3.0 3,682,083 + -0.2 3,682,084 - -3.1 3,682,086 + +0.0 3,682,086 + +0.4 3,682,086 + -1.8 3,682,181 - -3.0 3,682,221 + +0.1 3,682,221 + -0.2 3,682,230 - -2.1 3,682,249 + -0.2 3,682,250 - +1.8 3,682,250 - -3.5 3,682,329 + +2.8 3,682,329 + -4.2 3,682,329 + -0.5 3,682,329 + -0.7 3,682,329 + -0.1 3,682,330 - +3.8 3,682,362 + -1.3 3,682,362 + +3.1 3,682,363 - -0.6 3,682,363 - +1.8 3,682,363 - -1.3 3,682,363 - -0.2 3,682,375 + -2.1 3,682,498 - Psyr_3078 0.17 +3.5 3,682,498 - Psyr_3078 0.17 +2.6 3,682,498 - Psyr_3078 0.17 +1.3 3,682,589 - Psyr_3078 0.24 +3.6 3,682,813 + Psyr_3078 0.42 -3.1 3,682,814 - Psyr_3078 0.43 -1.4 3,683,073 + Psyr_3078 0.63 -1.2 3,683,073 + Psyr_3078 0.63 -1.4 3,683,073 + Psyr_3078 0.63 -1.9 3,683,073 + Psyr_3078 0.63 -0.5 3,683,073 + Psyr_3078 0.63 +3.5 3,683,073 + Psyr_3078 0.63 -2.8 3,683,074 - Psyr_3078 0.63 -1.8 3,683,074 - Psyr_3078 0.63 -2.1 3,683,074 - Psyr_3078 0.63 -2.1 3,683,074 - Psyr_3078 0.63 -0.9 3,683,140 + Psyr_3078 0.69 -0.2 3,683,342 + Psyr_3078 0.85 +3.4 3,683,343 - Psyr_3078 0.85 -2.3 3,683,346 + Psyr_3078 0.85 +1.2 3,683,406 + -0.4 3,683,406 + -0.3 3,683,406 + -0.2 3,683,406 + -1.2 3,683,407 - -0.2 3,683,407 - -3.2 3,683,407 - +2.6 3,683,407 - +3.7 3,683,407 - -0.6 3,683,572 + +0.3 3,683,572 + -1.7 3,683,572 + +3.2 3,683,572 + +0.8 3,683,573 - -0.4 3,683,573 - +3.8 3,683,573 - -3.0 3,684,085 + +0.1 3,684,085 + -1.4 3,684,085 + +2.6 3,684,085 + +1.8 3,684,086 - +2.8 3,684,086 - -3.7 3,684,086 - -0.7 3,684,086 - +1.8 3,684,101 - +3.0 3,684,101 - -1.0 3,684,123 + +1.8 3,684,123 + -2.6 3,684,124 - +4.1 3,684,124 - +2.4 3,684,149 - -2.0 3,684,257 + -0.8 3,684,518 - Psyr_3080 0.16 -1.4
Or see this region's nucleotide sequence