Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3049 and Psyr_3050 are separated by 67 nucleotides Psyr_3050 and Psyr_3051 are separated by 125 nucleotides
Psyr_3049: Psyr_3049 - Pirin, N-terminal, at 3,646,873 to 3,647,709
_3049
Psyr_3050: Psyr_3050 - Aldo/keto reductase, at 3,647,777 to 3,648,640
_3050
Psyr_3051: Psyr_3051 - transcriptional regulator, LysR family, at 3,648,766 to 3,649,662
_3051
Position (kb)
3647
3648
3649 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3646.779 kb on + strand at 3646.779 kb on + strand at 3646.800 kb on + strand at 3646.800 kb on + strand at 3646.800 kb on + strand at 3646.801 kb on - strand at 3646.801 kb on - strand at 3646.803 kb on - strand at 3646.832 kb on + strand at 3646.921 kb on - strand at 3647.080 kb on + strand, within Psyr_3049 at 3647.080 kb on + strand, within Psyr_3049 at 3647.080 kb on + strand, within Psyr_3049 at 3647.080 kb on + strand, within Psyr_3049 at 3647.080 kb on + strand, within Psyr_3049 at 3647.081 kb on - strand, within Psyr_3049 at 3647.081 kb on - strand, within Psyr_3049 at 3647.081 kb on - strand, within Psyr_3049 at 3647.081 kb on - strand, within Psyr_3049 at 3647.081 kb on - strand, within Psyr_3049 at 3647.104 kb on + strand, within Psyr_3049 at 3647.104 kb on + strand, within Psyr_3049 at 3647.104 kb on + strand, within Psyr_3049 at 3647.104 kb on + strand, within Psyr_3049 at 3647.276 kb on + strand, within Psyr_3049 at 3647.276 kb on + strand, within Psyr_3049 at 3647.276 kb on + strand, within Psyr_3049 at 3647.276 kb on + strand, within Psyr_3049 at 3647.276 kb on + strand, within Psyr_3049 at 3647.276 kb on + strand, within Psyr_3049 at 3647.277 kb on - strand, within Psyr_3049 at 3647.277 kb on - strand, within Psyr_3049 at 3647.277 kb on - strand, within Psyr_3049 at 3647.278 kb on + strand, within Psyr_3049 at 3647.278 kb on + strand, within Psyr_3049 at 3647.279 kb on - strand, within Psyr_3049 at 3647.279 kb on - strand, within Psyr_3049 at 3647.380 kb on + strand, within Psyr_3049 at 3647.381 kb on - strand, within Psyr_3049 at 3647.390 kb on + strand, within Psyr_3049 at 3647.391 kb on - strand, within Psyr_3049 at 3647.391 kb on - strand, within Psyr_3049 at 3647.397 kb on - strand, within Psyr_3049 at 3647.440 kb on - strand, within Psyr_3049 at 3647.444 kb on + strand, within Psyr_3049 at 3647.445 kb on - strand, within Psyr_3049 at 3647.617 kb on + strand, within Psyr_3049 at 3647.617 kb on + strand, within Psyr_3049 at 3647.666 kb on + strand at 3647.667 kb on - strand at 3647.667 kb on - strand at 3647.770 kb on + strand at 3647.771 kb on - strand at 3647.771 kb on - strand at 3647.778 kb on + strand at 3647.779 kb on - strand at 3648.015 kb on + strand, within Psyr_3050 at 3648.015 kb on + strand, within Psyr_3050 at 3648.015 kb on + strand, within Psyr_3050 at 3648.015 kb on + strand, within Psyr_3050 at 3648.015 kb on + strand, within Psyr_3050 at 3648.015 kb on + strand, within Psyr_3050 at 3648.016 kb on - strand, within Psyr_3050 at 3648.016 kb on - strand, within Psyr_3050 at 3648.079 kb on - strand, within Psyr_3050 at 3648.079 kb on - strand, within Psyr_3050 at 3648.454 kb on - strand, within Psyr_3050 at 3648.479 kb on + strand, within Psyr_3050 at 3648.577 kb on - strand at 3648.616 kb on + strand at 3648.616 kb on + strand at 3648.616 kb on + strand at 3648.616 kb on + strand at 3648.617 kb on - strand at 3648.617 kb on - strand at 3648.617 kb on - strand at 3648.617 kb on - strand at 3648.617 kb on - strand at 3648.617 kb on - strand at 3648.674 kb on + strand at 3648.675 kb on - strand at 3648.676 kb on + strand at 3648.693 kb on - strand at 3648.724 kb on + strand at 3648.963 kb on + strand, within Psyr_3051 at 3648.964 kb on - strand, within Psyr_3051 at 3648.973 kb on - strand, within Psyr_3051 at 3648.973 kb on - strand, within Psyr_3051 at 3649.120 kb on + strand, within Psyr_3051 at 3649.286 kb on - strand, within Psyr_3051 at 3649.384 kb on + strand, within Psyr_3051 at 3649.588 kb on + strand at 3649.594 kb on + strand at 3649.595 kb on - strand at 3649.595 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,646,779 + +1.8 3,646,779 + -0.2 3,646,800 + -2.2 3,646,800 + -1.8 3,646,800 + -1.5 3,646,801 - -1.8 3,646,801 - -0.4 3,646,803 - +1.8 3,646,832 + -2.6 3,646,921 - +2.1 3,647,080 + Psyr_3049 0.25 +3.0 3,647,080 + Psyr_3049 0.25 -0.1 3,647,080 + Psyr_3049 0.25 +1.4 3,647,080 + Psyr_3049 0.25 +1.4 3,647,080 + Psyr_3049 0.25 -1.6 3,647,081 - Psyr_3049 0.25 +1.4 3,647,081 - Psyr_3049 0.25 +0.8 3,647,081 - Psyr_3049 0.25 +1.8 3,647,081 - Psyr_3049 0.25 -2.4 3,647,081 - Psyr_3049 0.25 -2.4 3,647,104 + Psyr_3049 0.28 -1.2 3,647,104 + Psyr_3049 0.28 +2.1 3,647,104 + Psyr_3049 0.28 +1.4 3,647,104 + Psyr_3049 0.28 +1.4 3,647,276 + Psyr_3049 0.48 -3.1 3,647,276 + Psyr_3049 0.48 -1.2 3,647,276 + Psyr_3049 0.48 +3.2 3,647,276 + Psyr_3049 0.48 +1.8 3,647,276 + Psyr_3049 0.48 -0.2 3,647,276 + Psyr_3049 0.48 +0.5 3,647,277 - Psyr_3049 0.48 -2.8 3,647,277 - Psyr_3049 0.48 +1.4 3,647,277 - Psyr_3049 0.48 -1.4 3,647,278 + Psyr_3049 0.48 +0.3 3,647,278 + Psyr_3049 0.48 -0.5 3,647,279 - Psyr_3049 0.49 -1.7 3,647,279 - Psyr_3049 0.49 -1.1 3,647,380 + Psyr_3049 0.61 +1.8 3,647,381 - Psyr_3049 0.61 -0.7 3,647,390 + Psyr_3049 0.62 -0.7 3,647,391 - Psyr_3049 0.62 +2.8 3,647,391 - Psyr_3049 0.62 +1.4 3,647,397 - Psyr_3049 0.63 -0.5 3,647,440 - Psyr_3049 0.68 +3.7 3,647,444 + Psyr_3049 0.68 +0.8 3,647,445 - Psyr_3049 0.68 +0.4 3,647,617 + Psyr_3049 0.89 -0.9 3,647,617 + Psyr_3049 0.89 -3.3 3,647,666 + -0.4 3,647,667 - +1.8 3,647,667 - +0.9 3,647,770 + -0.2 3,647,771 - +3.2 3,647,771 - -1.5 3,647,778 + +0.5 3,647,779 - -2.8 3,648,015 + Psyr_3050 0.28 +1.6 3,648,015 + Psyr_3050 0.28 -1.3 3,648,015 + Psyr_3050 0.28 +0.8 3,648,015 + Psyr_3050 0.28 +1.8 3,648,015 + Psyr_3050 0.28 +2.1 3,648,015 + Psyr_3050 0.28 -0.2 3,648,016 - Psyr_3050 0.28 +1.8 3,648,016 - Psyr_3050 0.28 +2.6 3,648,079 - Psyr_3050 0.35 +2.6 3,648,079 - Psyr_3050 0.35 +3.1 3,648,454 - Psyr_3050 0.78 +0.0 3,648,479 + Psyr_3050 0.81 +2.4 3,648,577 - -2.0 3,648,616 + +0.1 3,648,616 + -0.9 3,648,616 + +2.8 3,648,616 + +2.6 3,648,617 - -2.9 3,648,617 - -0.5 3,648,617 - +3.0 3,648,617 - +3.5 3,648,617 - +2.6 3,648,617 - +4.3 3,648,674 + -2.2 3,648,675 - +0.8 3,648,676 + +2.1 3,648,693 - +2.8 3,648,724 + -0.2 3,648,963 + Psyr_3051 0.22 -0.3 3,648,964 - Psyr_3051 0.22 -0.6 3,648,973 - Psyr_3051 0.23 +0.8 3,648,973 - Psyr_3051 0.23 +0.6 3,649,120 + Psyr_3051 0.39 -0.7 3,649,286 - Psyr_3051 0.58 +0.4 3,649,384 + Psyr_3051 0.69 +0.7 3,649,588 + -3.8 3,649,594 + +2.6 3,649,595 - -1.5 3,649,595 - -0.2
Or see this region's nucleotide sequence