Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2989 and Psyr_2990 are separated by 312 nucleotides Psyr_2990 and Psyr_2991 are separated by 160 nucleotides
Psyr_2989: Psyr_2989 - 6-phosphogluconate dehydrogenase (decarboxylating), at 3,580,939 to 3,581,913
_2989
Psyr_2990: Psyr_2990 - conserved hypothetical protein, at 3,582,226 to 3,582,399
_2990
Psyr_2991: Psyr_2991 - GCN5-related N-acetyltransferase, at 3,582,560 to 3,583,051
_2991
Position (kb)
3582
3583 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 3581.387 kb on + strand, within Psyr_2989 at 3581.387 kb on + strand, within Psyr_2989 at 3581.387 kb on + strand, within Psyr_2989 at 3581.387 kb on + strand, within Psyr_2989 at 3581.523 kb on - strand, within Psyr_2989 at 3581.523 kb on - strand, within Psyr_2989 at 3581.570 kb on + strand, within Psyr_2989 at 3581.570 kb on + strand, within Psyr_2989 at 3581.570 kb on + strand, within Psyr_2989 at 3581.570 kb on + strand, within Psyr_2989 at 3581.571 kb on - strand, within Psyr_2989 at 3581.571 kb on - strand, within Psyr_2989 at 3581.571 kb on - strand, within Psyr_2989 at 3581.571 kb on - strand, within Psyr_2989 at 3581.621 kb on + strand, within Psyr_2989 at 3581.622 kb on - strand, within Psyr_2989 at 3581.622 kb on - strand, within Psyr_2989 at 3581.622 kb on - strand, within Psyr_2989 at 3581.622 kb on - strand, within Psyr_2989 at 3581.828 kb on + strand at 3581.829 kb on - strand at 3581.829 kb on - strand at 3581.926 kb on + strand at 3581.926 kb on + strand at 3581.927 kb on - strand at 3581.988 kb on - strand at 3582.071 kb on - strand at 3582.149 kb on - strand at 3582.236 kb on + strand at 3582.236 kb on + strand at 3582.236 kb on + strand at 3582.236 kb on + strand at 3582.236 kb on + strand at 3582.236 kb on + strand at 3582.236 kb on + strand at 3582.237 kb on - strand at 3582.237 kb on - strand at 3582.237 kb on - strand at 3582.237 kb on - strand at 3582.237 kb on - strand at 3582.239 kb on + strand at 3582.239 kb on + strand at 3582.265 kb on + strand, within Psyr_2990 at 3582.299 kb on + strand, within Psyr_2990 at 3582.300 kb on - strand, within Psyr_2990 at 3582.409 kb on + strand at 3582.409 kb on + strand at 3582.409 kb on + strand at 3582.561 kb on + strand at 3582.612 kb on + strand, within Psyr_2991 at 3582.613 kb on - strand, within Psyr_2991 at 3582.613 kb on - strand, within Psyr_2991 at 3582.613 kb on - strand, within Psyr_2991 at 3582.613 kb on - strand, within Psyr_2991 at 3582.613 kb on - strand, within Psyr_2991 at 3582.613 kb on - strand, within Psyr_2991 at 3582.883 kb on - strand, within Psyr_2991 at 3582.967 kb on - strand, within Psyr_2991 at 3582.967 kb on - strand, within Psyr_2991 at 3583.014 kb on + strand at 3583.015 kb on - strand at 3583.086 kb on + strand at 3583.149 kb on - strand at 3583.149 kb on - strand at 3583.184 kb on + strand at 3583.184 kb on + strand at 3583.220 kb on - strand at 3583.284 kb on + strand at 3583.284 kb on + strand at 3583.285 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,581,387 + Psyr_2989 0.46 -1.1 3,581,387 + Psyr_2989 0.46 +4.0 3,581,387 + Psyr_2989 0.46 +2.5 3,581,387 + Psyr_2989 0.46 -0.1 3,581,523 - Psyr_2989 0.60 -1.4 3,581,523 - Psyr_2989 0.60 -1.5 3,581,570 + Psyr_2989 0.65 -4.9 3,581,570 + Psyr_2989 0.65 +2.3 3,581,570 + Psyr_2989 0.65 +0.7 3,581,570 + Psyr_2989 0.65 -0.9 3,581,571 - Psyr_2989 0.65 -2.3 3,581,571 - Psyr_2989 0.65 -0.3 3,581,571 - Psyr_2989 0.65 +0.7 3,581,571 - Psyr_2989 0.65 -3.5 3,581,621 + Psyr_2989 0.70 -1.6 3,581,622 - Psyr_2989 0.70 -0.3 3,581,622 - Psyr_2989 0.70 -1.4 3,581,622 - Psyr_2989 0.70 -1.2 3,581,622 - Psyr_2989 0.70 +1.3 3,581,828 + -2.2 3,581,829 - -1.5 3,581,829 - +1.3 3,581,926 + +0.2 3,581,926 + -1.0 3,581,927 - +0.7 3,581,988 - +3.2 3,582,071 - -0.8 3,582,149 - -3.3 3,582,236 + +1.3 3,582,236 + +3.1 3,582,236 + -0.8 3,582,236 + -1.0 3,582,236 + -1.3 3,582,236 + +1.1 3,582,236 + +2.9 3,582,237 - +1.7 3,582,237 - -3.0 3,582,237 - +1.7 3,582,237 - -0.3 3,582,237 - +2.7 3,582,239 + +2.5 3,582,239 + +0.7 3,582,265 + Psyr_2990 0.22 -1.8 3,582,299 + Psyr_2990 0.42 -1.8 3,582,300 - Psyr_2990 0.43 -1.1 3,582,409 + +0.2 3,582,409 + -0.5 3,582,409 + +0.0 3,582,561 + -3.0 3,582,612 + Psyr_2991 0.11 +2.9 3,582,613 - Psyr_2991 0.11 +1.3 3,582,613 - Psyr_2991 0.11 -0.8 3,582,613 - Psyr_2991 0.11 -1.3 3,582,613 - Psyr_2991 0.11 +1.5 3,582,613 - Psyr_2991 0.11 -1.6 3,582,613 - Psyr_2991 0.11 -1.2 3,582,883 - Psyr_2991 0.66 +3.6 3,582,967 - Psyr_2991 0.83 -0.7 3,582,967 - Psyr_2991 0.83 +3.6 3,583,014 + -0.8 3,583,015 - +0.7 3,583,086 + +2.1 3,583,149 - +2.7 3,583,149 - -1.4 3,583,184 + -1.7 3,583,184 + -0.8 3,583,220 - +0.9 3,583,284 + +1.7 3,583,284 + +0.7 3,583,285 - -0.6
Or see this region's nucleotide sequence