Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2983 and Psyr_2984 are separated by 14 nucleotides Psyr_2984 and Psyr_2985 are separated by 24 nucleotides Psyr_2985 and Psyr_2986 are separated by 0 nucleotides Psyr_2986 and Psyr_2987 are separated by 51 nucleotides Psyr_2987 and Psyr_2988 overlap by 8 nucleotides
Psyr_2983: Psyr_2983 - conserved domain protein, at 3,575,522 to 3,575,827
_2983
Psyr_2984: Psyr_2984 - conserved hypothetical protein, at 3,575,842 to 3,576,024
_2984
Psyr_2985: Psyr_2985 - DNA-N1-methyladenine dioxygenase, at 3,576,049 to 3,576,735
_2985
Psyr_2986: Psyr_2986 - Glycoside hydrolase, family 15, at 3,576,736 to 3,578,553
_2986
Psyr_2987: Psyr_2987 - Cof protein:HAD-superfamily hydrolase, subfamily IIB, at 3,578,605 to 3,579,414
_2987
Psyr_2988: Psyr_2988 - glucose-6-phosphate 1-dehydrogenase, at 3,579,407 to 3,580,939
_2988
Position (kb)
3576
3577
3578
3579 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 3575.884 kb on + strand, within Psyr_2984 at 3575.991 kb on + strand, within Psyr_2984 at 3575.991 kb on + strand, within Psyr_2984 at 3575.992 kb on - strand, within Psyr_2984 at 3575.992 kb on - strand, within Psyr_2984 at 3576.025 kb on + strand at 3576.025 kb on + strand at 3576.368 kb on + strand, within Psyr_2985 at 3576.368 kb on + strand, within Psyr_2985 at 3576.368 kb on + strand, within Psyr_2985 at 3576.451 kb on - strand, within Psyr_2985 at 3576.451 kb on - strand, within Psyr_2985 at 3576.493 kb on + strand, within Psyr_2985 at 3576.782 kb on - strand at 3576.782 kb on - strand at 3576.807 kb on + strand at 3576.807 kb on + strand at 3576.807 kb on + strand at 3576.808 kb on - strand at 3576.875 kb on + strand at 3576.876 kb on - strand at 3576.965 kb on + strand, within Psyr_2986 at 3576.965 kb on + strand, within Psyr_2986 at 3576.965 kb on + strand, within Psyr_2986 at 3576.965 kb on + strand, within Psyr_2986 at 3576.965 kb on + strand, within Psyr_2986 at 3576.966 kb on - strand, within Psyr_2986 at 3576.966 kb on - strand, within Psyr_2986 at 3576.966 kb on - strand, within Psyr_2986 at 3576.966 kb on - strand, within Psyr_2986 at 3577.181 kb on + strand, within Psyr_2986 at 3577.181 kb on + strand, within Psyr_2986 at 3577.181 kb on + strand, within Psyr_2986 at 3577.181 kb on + strand, within Psyr_2986 at 3577.182 kb on - strand, within Psyr_2986 at 3577.182 kb on - strand, within Psyr_2986 at 3577.182 kb on - strand, within Psyr_2986 at 3577.182 kb on - strand, within Psyr_2986 at 3577.384 kb on + strand, within Psyr_2986 at 3577.384 kb on + strand, within Psyr_2986 at 3577.384 kb on + strand, within Psyr_2986 at 3577.385 kb on - strand, within Psyr_2986 at 3577.385 kb on - strand, within Psyr_2986 at 3577.385 kb on - strand, within Psyr_2986 at 3577.610 kb on + strand, within Psyr_2986 at 3577.611 kb on - strand, within Psyr_2986 at 3577.643 kb on + strand at 3577.643 kb on + strand, within Psyr_2986 at 3577.643 kb on + strand, within Psyr_2986 at 3577.643 kb on + strand, within Psyr_2986 at 3577.644 kb on - strand, within Psyr_2986 at 3577.722 kb on - strand, within Psyr_2986 at 3577.776 kb on - strand, within Psyr_2986 at 3577.851 kb on + strand, within Psyr_2986 at 3577.859 kb on + strand, within Psyr_2986 at 3577.859 kb on + strand, within Psyr_2986 at 3577.859 kb on + strand, within Psyr_2986 at 3577.860 kb on - strand, within Psyr_2986 at 3577.878 kb on + strand, within Psyr_2986 at 3577.878 kb on + strand, within Psyr_2986 at 3577.895 kb on - strand, within Psyr_2986 at 3577.895 kb on - strand, within Psyr_2986 at 3578.531 kb on + strand at 3578.531 kb on + strand at 3578.621 kb on + strand at 3578.621 kb on + strand at 3578.661 kb on + strand at 3578.668 kb on - strand at 3578.801 kb on + strand, within Psyr_2987 at 3578.801 kb on + strand, within Psyr_2987 at 3578.861 kb on + strand, within Psyr_2987 at 3578.865 kb on - strand, within Psyr_2987 at 3578.985 kb on - strand, within Psyr_2987 at 3579.011 kb on + strand, within Psyr_2987 at 3579.011 kb on + strand, within Psyr_2987 at 3579.011 kb on + strand, within Psyr_2987 at 3579.011 kb on + strand, within Psyr_2987 at 3579.011 kb on + strand, within Psyr_2987 at 3579.012 kb on - strand, within Psyr_2987 at 3579.012 kb on - strand, within Psyr_2987 at 3579.012 kb on - strand, within Psyr_2987 at 3579.047 kb on + strand, within Psyr_2987 at 3579.397 kb on + strand at 3579.397 kb on + strand at 3579.397 kb on + strand at 3579.398 kb on - strand at 3579.490 kb on - strand at 3579.490 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,575,884 + Psyr_2984 0.23 -2.6 3,575,991 + Psyr_2984 0.81 +0.7 3,575,991 + Psyr_2984 0.81 -0.9 3,575,992 - Psyr_2984 0.82 +2.3 3,575,992 - Psyr_2984 0.82 -0.8 3,576,025 + +2.1 3,576,025 + +0.7 3,576,368 + Psyr_2985 0.46 +3.4 3,576,368 + Psyr_2985 0.46 -0.8 3,576,368 + Psyr_2985 0.46 +3.1 3,576,451 - Psyr_2985 0.59 -0.8 3,576,451 - Psyr_2985 0.59 +2.1 3,576,493 + Psyr_2985 0.65 +2.3 3,576,782 - +4.0 3,576,782 - -1.7 3,576,807 + -3.1 3,576,807 + -3.0 3,576,807 + +2.5 3,576,808 - +1.7 3,576,875 + +2.5 3,576,876 - -1.3 3,576,965 + Psyr_2986 0.13 +2.4 3,576,965 + Psyr_2986 0.13 -0.3 3,576,965 + Psyr_2986 0.13 -0.2 3,576,965 + Psyr_2986 0.13 -1.8 3,576,965 + Psyr_2986 0.13 +2.1 3,576,966 - Psyr_2986 0.13 +2.5 3,576,966 - Psyr_2986 0.13 +1.3 3,576,966 - Psyr_2986 0.13 -0.3 3,576,966 - Psyr_2986 0.13 +0.6 3,577,181 + Psyr_2986 0.24 +2.5 3,577,181 + Psyr_2986 0.24 -0.7 3,577,181 + Psyr_2986 0.24 +0.7 3,577,181 + Psyr_2986 0.24 +2.1 3,577,182 - Psyr_2986 0.25 -1.4 3,577,182 - Psyr_2986 0.25 -0.8 3,577,182 - Psyr_2986 0.25 +2.9 3,577,182 - Psyr_2986 0.25 -0.6 3,577,384 + Psyr_2986 0.36 -3.6 3,577,384 + Psyr_2986 0.36 +3.3 3,577,384 + Psyr_2986 0.36 +1.3 3,577,385 - Psyr_2986 0.36 -2.0 3,577,385 - Psyr_2986 0.36 +3.2 3,577,385 - Psyr_2986 0.36 -1.8 3,577,610 + Psyr_2986 0.48 +3.2 3,577,611 - Psyr_2986 0.48 -0.3 3,577,643 + +1.3 3,577,643 + Psyr_2986 0.50 +1.7 3,577,643 + Psyr_2986 0.50 -2.0 3,577,643 + Psyr_2986 0.50 -2.1 3,577,644 - Psyr_2986 0.50 +2.7 3,577,722 - Psyr_2986 0.54 +3.3 3,577,776 - Psyr_2986 0.57 +1.7 3,577,851 + Psyr_2986 0.61 -0.3 3,577,859 + Psyr_2986 0.62 -2.4 3,577,859 + Psyr_2986 0.62 +1.3 3,577,859 + Psyr_2986 0.62 -3.5 3,577,860 - Psyr_2986 0.62 +0.7 3,577,878 + Psyr_2986 0.63 +1.3 3,577,878 + Psyr_2986 0.63 -4.2 3,577,895 - Psyr_2986 0.64 +0.8 3,577,895 - Psyr_2986 0.64 -1.4 3,578,531 + -0.3 3,578,531 + +3.6 3,578,621 + -5.5 3,578,621 + -0.7 3,578,661 + -0.3 3,578,668 - +2.3 3,578,801 + Psyr_2987 0.24 +0.7 3,578,801 + Psyr_2987 0.24 -2.6 3,578,861 + Psyr_2987 0.32 -1.8 3,578,865 - Psyr_2987 0.32 +0.7 3,578,985 - Psyr_2987 0.47 -0.6 3,579,011 + Psyr_2987 0.50 +2.5 3,579,011 + Psyr_2987 0.50 +3.1 3,579,011 + Psyr_2987 0.50 +0.1 3,579,011 + Psyr_2987 0.50 -0.3 3,579,011 + Psyr_2987 0.50 -0.3 3,579,012 - Psyr_2987 0.50 -0.9 3,579,012 - Psyr_2987 0.50 +4.0 3,579,012 - Psyr_2987 0.50 -0.3 3,579,047 + Psyr_2987 0.55 -3.2 3,579,397 + +1.3 3,579,397 + -2.4 3,579,397 + +0.2 3,579,398 - -2.8 3,579,490 - -2.9 3,579,490 - -0.3
Or see this region's nucleotide sequence