Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2980 and Psyr_2981 are separated by 372 nucleotides Psyr_2981 and Psyr_2982 are separated by 55 nucleotides Psyr_2982 and Psyr_2983 are separated by 167 nucleotides Psyr_2983 and Psyr_2984 are separated by 14 nucleotides
Psyr_2980: Psyr_2980 - UDP-glucose pyrophosphorylase, at 3,573,452 to 3,574,291
_2980
Psyr_2981: Psyr_2981 - hypothetical protein, at 3,574,664 to 3,574,945
_2981
Psyr_2982: Psyr_2982 - conserved hypothetical protein, at 3,575,001 to 3,575,354
_2982
Psyr_2983: Psyr_2983 - conserved domain protein, at 3,575,522 to 3,575,827
_2983
Psyr_2984: Psyr_2984 - conserved hypothetical protein, at 3,575,842 to 3,576,024
_2984
Position (kb)
3574
3575 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 3573.783 kb on + strand, within Psyr_2980 at 3573.783 kb on + strand, within Psyr_2980 at 3573.783 kb on + strand, within Psyr_2980 at 3573.784 kb on - strand, within Psyr_2980 at 3573.784 kb on - strand, within Psyr_2980 at 3573.784 kb on - strand, within Psyr_2980 at 3574.084 kb on - strand, within Psyr_2980 at 3574.180 kb on - strand, within Psyr_2980 at 3574.333 kb on + strand at 3574.333 kb on + strand at 3574.334 kb on - strand at 3574.353 kb on + strand at 3574.353 kb on + strand at 3574.354 kb on - strand at 3574.354 kb on - strand at 3574.386 kb on + strand at 3574.394 kb on + strand at 3574.394 kb on + strand at 3574.401 kb on - strand at 3574.406 kb on + strand at 3574.406 kb on + strand at 3574.406 kb on + strand at 3574.406 kb on + strand at 3574.406 kb on + strand at 3574.407 kb on - strand at 3574.407 kb on - strand at 3574.407 kb on - strand at 3574.407 kb on - strand at 3574.407 kb on - strand at 3574.407 kb on - strand at 3574.426 kb on - strand at 3574.438 kb on + strand at 3574.438 kb on + strand at 3574.438 kb on + strand at 3574.438 kb on + strand at 3574.439 kb on - strand at 3574.439 kb on - strand at 3574.439 kb on - strand at 3574.439 kb on - strand at 3574.461 kb on - strand at 3574.471 kb on - strand at 3574.521 kb on + strand at 3574.521 kb on + strand at 3574.521 kb on + strand at 3574.522 kb on - strand at 3574.522 kb on - strand at 3574.522 kb on - strand at 3574.522 kb on - strand at 3574.522 kb on - strand at 3574.573 kb on + strand at 3574.573 kb on + strand at 3574.574 kb on - strand at 3574.574 kb on - strand at 3574.574 kb on - strand at 3574.605 kb on + strand at 3574.605 kb on + strand at 3574.605 kb on + strand at 3574.605 kb on + strand at 3574.605 kb on + strand at 3574.606 kb on - strand at 3574.606 kb on - strand at 3574.609 kb on - strand at 3574.646 kb on + strand at 3574.730 kb on + strand, within Psyr_2981 at 3574.730 kb on + strand, within Psyr_2981 at 3574.943 kb on + strand at 3574.944 kb on - strand at 3575.118 kb on + strand, within Psyr_2982 at 3575.282 kb on + strand, within Psyr_2982 at 3575.282 kb on + strand, within Psyr_2982 at 3575.282 kb on + strand, within Psyr_2982 at 3575.282 kb on + strand, within Psyr_2982 at 3575.282 kb on + strand, within Psyr_2982 at 3575.283 kb on - strand, within Psyr_2982 at 3575.283 kb on - strand, within Psyr_2982 at 3575.283 kb on - strand, within Psyr_2982 at 3575.334 kb on + strand at 3575.334 kb on + strand at 3575.337 kb on + strand at 3575.337 kb on + strand at 3575.337 kb on + strand at 3575.338 kb on - strand at 3575.338 kb on - strand at 3575.369 kb on + strand at 3575.369 kb on + strand at 3575.370 kb on - strand at 3575.370 kb on - strand at 3575.379 kb on - strand at 3575.399 kb on + strand at 3575.399 kb on + strand at 3575.399 kb on + strand at 3575.400 kb on - strand at 3575.400 kb on - strand at 3575.400 kb on - strand at 3575.442 kb on - strand at 3575.442 kb on - strand at 3575.533 kb on - strand at 3575.533 kb on - strand at 3575.596 kb on + strand, within Psyr_2983 at 3575.654 kb on + strand, within Psyr_2983 at 3575.654 kb on + strand, within Psyr_2983 at 3575.654 kb on + strand, within Psyr_2983 at 3575.654 kb on + strand, within Psyr_2983 at 3575.654 kb on + strand, within Psyr_2983 at 3575.655 kb on - strand, within Psyr_2983 at 3575.655 kb on - strand, within Psyr_2983 at 3575.655 kb on - strand, within Psyr_2983 at 3575.655 kb on - strand, within Psyr_2983 at 3575.655 kb on - strand, within Psyr_2983 at 3575.655 kb on - strand, within Psyr_2983 at 3575.655 kb on - strand, within Psyr_2983 at 3575.655 kb on - strand, within Psyr_2983 at 3575.655 kb on - strand, within Psyr_2983 at 3575.655 kb on - strand, within Psyr_2983 at 3575.655 kb on - strand, within Psyr_2983 at 3575.884 kb on + strand, within Psyr_2984
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,573,783 + Psyr_2980 0.39 -4.8 3,573,783 + Psyr_2980 0.39 +0.7 3,573,783 + Psyr_2980 0.39 -0.3 3,573,784 - Psyr_2980 0.40 -5.0 3,573,784 - Psyr_2980 0.40 -0.0 3,573,784 - Psyr_2980 0.40 +2.3 3,574,084 - Psyr_2980 0.75 -4.7 3,574,180 - Psyr_2980 0.87 -4.4 3,574,333 + +0.7 3,574,333 + +2.5 3,574,334 - +2.1 3,574,353 + -0.3 3,574,353 + -1.6 3,574,354 - +4.3 3,574,354 - +0.5 3,574,386 + +2.9 3,574,394 + +0.4 3,574,394 + -2.8 3,574,401 - +3.4 3,574,406 + -0.9 3,574,406 + +2.9 3,574,406 + -0.3 3,574,406 + -3.1 3,574,406 + -2.3 3,574,407 - -1.0 3,574,407 - +0.7 3,574,407 - -2.0 3,574,407 - -1.2 3,574,407 - -2.1 3,574,407 - +1.3 3,574,426 - -0.3 3,574,438 + -0.3 3,574,438 + -3.3 3,574,438 + -2.0 3,574,438 + -0.8 3,574,439 - +0.7 3,574,439 - +2.3 3,574,439 - +2.3 3,574,439 - +2.7 3,574,461 - +3.1 3,574,471 - -1.5 3,574,521 + +2.9 3,574,521 + -0.3 3,574,521 + +0.7 3,574,522 - -1.8 3,574,522 - -0.3 3,574,522 - +4.6 3,574,522 - -3.1 3,574,522 - -2.7 3,574,573 + -1.2 3,574,573 + +2.7 3,574,574 - -1.3 3,574,574 - +0.3 3,574,574 - -1.3 3,574,605 + +2.1 3,574,605 + +2.6 3,574,605 + -1.1 3,574,605 + +2.1 3,574,605 + +2.9 3,574,606 - -1.8 3,574,606 - +3.5 3,574,609 - -0.6 3,574,646 + +0.5 3,574,730 + Psyr_2981 0.23 +1.3 3,574,730 + Psyr_2981 0.23 +2.3 3,574,943 + +3.4 3,574,944 - -0.3 3,575,118 + Psyr_2982 0.33 -1.1 3,575,282 + Psyr_2982 0.79 -2.5 3,575,282 + Psyr_2982 0.79 -0.7 3,575,282 + Psyr_2982 0.79 -2.3 3,575,282 + Psyr_2982 0.79 +2.3 3,575,282 + Psyr_2982 0.79 +1.7 3,575,283 - Psyr_2982 0.80 +2.9 3,575,283 - Psyr_2982 0.80 -0.3 3,575,283 - Psyr_2982 0.80 -1.7 3,575,334 + +1.7 3,575,334 + +3.5 3,575,337 + +0.7 3,575,337 + -4.1 3,575,337 + -1.5 3,575,338 - -1.3 3,575,338 - -1.3 3,575,369 + +1.7 3,575,369 + +2.7 3,575,370 - +2.1 3,575,370 - -1.2 3,575,379 - +2.3 3,575,399 + -4.7 3,575,399 + +4.5 3,575,399 + +2.7 3,575,400 - +1.7 3,575,400 - +2.5 3,575,400 - -0.5 3,575,442 - -2.9 3,575,442 - -1.1 3,575,533 - +2.0 3,575,533 - +0.7 3,575,596 + Psyr_2983 0.24 -0.3 3,575,654 + Psyr_2983 0.43 +0.7 3,575,654 + Psyr_2983 0.43 -1.3 3,575,654 + Psyr_2983 0.43 +1.7 3,575,654 + Psyr_2983 0.43 -0.3 3,575,654 + Psyr_2983 0.43 +0.7 3,575,655 - Psyr_2983 0.43 +2.1 3,575,655 - Psyr_2983 0.43 +2.5 3,575,655 - Psyr_2983 0.43 +0.7 3,575,655 - Psyr_2983 0.43 +0.7 3,575,655 - Psyr_2983 0.43 +4.1 3,575,655 - Psyr_2983 0.43 +2.7 3,575,655 - Psyr_2983 0.43 -0.9 3,575,655 - Psyr_2983 0.43 +0.3 3,575,655 - Psyr_2983 0.43 +0.7 3,575,655 - Psyr_2983 0.43 -1.3 3,575,655 - Psyr_2983 0.43 +3.8 3,575,884 + Psyr_2984 0.23 -2.6
Or see this region's nucleotide sequence