Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2969 and Psyr_2970 are separated by 142 nucleotides Psyr_2970 and Psyr_2971 are separated by 486 nucleotides
Psyr_2969: Psyr_2969 - RND efflux system, outer membrane lipoprotein, NodT, at 3,560,826 to 3,562,223
_2969
Psyr_2970: Psyr_2970 - TPR repeat protein:TPR repeat protein, at 3,562,366 to 3,563,460
_2970
Psyr_2971: Psyr_2971 - H-NS family protein MvaT, at 3,563,947 to 3,564,309
_2971
Position (kb)
3562
3563
3564 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3561.930 kb on + strand, within Psyr_2969 at 3561.930 kb on + strand, within Psyr_2969 at 3561.930 kb on + strand, within Psyr_2969 at 3561.931 kb on - strand, within Psyr_2969 at 3561.931 kb on - strand, within Psyr_2969 at 3561.931 kb on - strand, within Psyr_2969 at 3561.931 kb on - strand, within Psyr_2969 at 3561.931 kb on - strand, within Psyr_2969 at 3561.931 kb on - strand, within Psyr_2969 at 3562.185 kb on + strand at 3562.185 kb on + strand at 3562.185 kb on + strand at 3562.185 kb on + strand at 3562.185 kb on + strand at 3562.185 kb on + strand at 3562.186 kb on - strand at 3562.186 kb on - strand at 3562.186 kb on - strand at 3562.186 kb on - strand at 3562.186 kb on - strand at 3562.186 kb on - strand at 3562.381 kb on - strand at 3562.519 kb on - strand, within Psyr_2970 at 3562.522 kb on + strand, within Psyr_2970 at 3562.522 kb on + strand, within Psyr_2970 at 3562.522 kb on + strand, within Psyr_2970 at 3562.523 kb on - strand, within Psyr_2970 at 3562.523 kb on - strand, within Psyr_2970 at 3562.523 kb on - strand, within Psyr_2970 at 3562.523 kb on - strand, within Psyr_2970 at 3562.523 kb on - strand, within Psyr_2970 at 3562.523 kb on - strand, within Psyr_2970 at 3562.862 kb on - strand, within Psyr_2970 at 3562.862 kb on - strand, within Psyr_2970 at 3562.958 kb on - strand, within Psyr_2970 at 3562.997 kb on + strand, within Psyr_2970 at 3563.057 kb on + strand, within Psyr_2970 at 3563.511 kb on - strand at 3563.648 kb on + strand at 3563.653 kb on + strand at 3563.653 kb on + strand at 3563.654 kb on - strand at 3563.654 kb on - strand at 3563.670 kb on + strand at 3563.739 kb on + strand at 3563.786 kb on + strand at 3563.787 kb on - strand at 3563.788 kb on + strand at 3563.788 kb on + strand at 3563.788 kb on + strand at 3563.789 kb on - strand at 3563.789 kb on - strand at 3563.792 kb on + strand at 3563.792 kb on + strand at 3563.908 kb on - strand at 3563.926 kb on + strand at 3563.926 kb on + strand at 3563.927 kb on - strand at 3563.927 kb on - strand at 3563.939 kb on + strand at 3563.939 kb on + strand at 3563.940 kb on - strand at 3563.941 kb on + strand at 3563.941 kb on + strand at 3563.941 kb on + strand at 3563.944 kb on + strand at 3563.944 kb on + strand at 3563.944 kb on + strand at 3563.944 kb on + strand at 3563.944 kb on + strand at 3563.945 kb on - strand at 3564.194 kb on - strand at 3564.194 kb on - strand, within Psyr_2971 at 3564.279 kb on - strand at 3564.279 kb on - strand at 3564.279 kb on - strand at 3564.353 kb on - strand at 3564.443 kb on + strand at 3564.445 kb on + strand at 3564.446 kb on - strand at 3564.446 kb on - strand at 3564.446 kb on - strand at 3564.446 kb on - strand at 3564.446 kb on - strand at 3564.454 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,561,930 + Psyr_2969 0.79 +2.5 3,561,930 + Psyr_2969 0.79 +2.5 3,561,930 + Psyr_2969 0.79 -1.9 3,561,931 - Psyr_2969 0.79 -0.3 3,561,931 - Psyr_2969 0.79 -0.3 3,561,931 - Psyr_2969 0.79 -0.4 3,561,931 - Psyr_2969 0.79 +2.1 3,561,931 - Psyr_2969 0.79 -0.3 3,561,931 - Psyr_2969 0.79 -0.3 3,562,185 + +2.3 3,562,185 + -3.6 3,562,185 + -0.3 3,562,185 + -2.7 3,562,185 + +0.7 3,562,185 + +1.3 3,562,186 - -4.5 3,562,186 - -2.0 3,562,186 - +3.1 3,562,186 - +1.7 3,562,186 - +0.7 3,562,186 - +0.7 3,562,381 - -0.3 3,562,519 - Psyr_2970 0.14 -2.3 3,562,522 + Psyr_2970 0.14 +1.3 3,562,522 + Psyr_2970 0.14 -3.4 3,562,522 + Psyr_2970 0.14 -1.4 3,562,523 - Psyr_2970 0.14 -1.6 3,562,523 - Psyr_2970 0.14 -1.6 3,562,523 - Psyr_2970 0.14 +1.7 3,562,523 - Psyr_2970 0.14 +1.7 3,562,523 - Psyr_2970 0.14 +2.3 3,562,523 - Psyr_2970 0.14 -0.3 3,562,862 - Psyr_2970 0.45 +3.2 3,562,862 - Psyr_2970 0.45 +0.7 3,562,958 - Psyr_2970 0.54 -2.7 3,562,997 + Psyr_2970 0.58 +2.3 3,563,057 + Psyr_2970 0.63 -3.6 3,563,511 - +1.7 3,563,648 + -1.8 3,563,653 + -1.1 3,563,653 + -0.6 3,563,654 - -2.6 3,563,654 - +3.1 3,563,670 + -0.3 3,563,739 + +3.2 3,563,786 + +3.1 3,563,787 - -2.7 3,563,788 + +3.4 3,563,788 + +1.0 3,563,788 + +0.8 3,563,789 - +3.1 3,563,789 - +1.7 3,563,792 + -3.0 3,563,792 + +2.3 3,563,908 - -1.1 3,563,926 + -2.0 3,563,926 + -2.5 3,563,927 - -0.3 3,563,927 - -0.3 3,563,939 + +2.9 3,563,939 + +1.7 3,563,940 - +2.3 3,563,941 + -0.4 3,563,941 + -3.7 3,563,941 + +1.3 3,563,944 + -1.6 3,563,944 + +1.3 3,563,944 + +2.1 3,563,944 + -0.3 3,563,944 + +1.3 3,563,945 - -1.7 3,564,194 - +1.7 3,564,194 - Psyr_2971 0.68 -2.8 3,564,279 - +1.3 3,564,279 - -0.3 3,564,279 - -0.3 3,564,353 - +2.3 3,564,443 + -1.5 3,564,445 + +1.3 3,564,446 - -1.4 3,564,446 - +4.1 3,564,446 - -3.4 3,564,446 - -0.7 3,564,446 - +2.1 3,564,454 - -0.3
Or see this region's nucleotide sequence