Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2957 and Psyr_2958 are separated by 373 nucleotides Psyr_2958 and Psyr_2959 overlap by 4 nucleotides Psyr_2959 and Psyr_2960 are separated by 4 nucleotides
Psyr_2957: Psyr_2957 - Glutathione-dependent formaldehyde-activating, GFA, at 3,544,623 to 3,545,243
_2957
Psyr_2958: Psyr_2958 - extracellular solute-binding protein, family 5, at 3,545,617 to 3,547,248
_2958
Psyr_2959: Psyr_2959 - Binding-protein-dependent transport systems inner membrane component, at 3,547,245 to 3,548,195
_2959
Psyr_2960: Psyr_2960 - Binding-protein-dependent transport systems inner membrane component, at 3,548,200 to 3,549,132
_2960
Position (kb)
3545
3546
3547
3548 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3544.647 kb on + strand at 3544.648 kb on - strand at 3544.649 kb on + strand at 3544.650 kb on - strand at 3544.659 kb on + strand at 3544.659 kb on + strand at 3544.659 kb on + strand at 3544.660 kb on - strand at 3544.932 kb on - strand, within Psyr_2957 at 3544.970 kb on + strand, within Psyr_2957 at 3544.970 kb on + strand, within Psyr_2957 at 3544.970 kb on + strand, within Psyr_2957 at 3544.970 kb on + strand, within Psyr_2957 at 3544.970 kb on + strand, within Psyr_2957 at 3544.971 kb on - strand, within Psyr_2957 at 3544.971 kb on - strand, within Psyr_2957 at 3544.971 kb on - strand, within Psyr_2957 at 3544.974 kb on + strand, within Psyr_2957 at 3544.974 kb on + strand, within Psyr_2957 at 3544.974 kb on + strand, within Psyr_2957 at 3544.974 kb on + strand, within Psyr_2957 at 3544.974 kb on + strand, within Psyr_2957 at 3544.974 kb on + strand, within Psyr_2957 at 3544.974 kb on + strand, within Psyr_2957 at 3544.974 kb on + strand, within Psyr_2957 at 3544.974 kb on + strand, within Psyr_2957 at 3544.975 kb on - strand, within Psyr_2957 at 3544.975 kb on - strand, within Psyr_2957 at 3544.975 kb on - strand, within Psyr_2957 at 3544.975 kb on - strand, within Psyr_2957 at 3544.975 kb on - strand, within Psyr_2957 at 3544.975 kb on - strand, within Psyr_2957 at 3544.975 kb on - strand, within Psyr_2957 at 3544.975 kb on - strand, within Psyr_2957 at 3544.975 kb on - strand, within Psyr_2957 at 3544.975 kb on - strand, within Psyr_2957 at 3544.975 kb on - strand, within Psyr_2957 at 3544.975 kb on - strand, within Psyr_2957 at 3545.163 kb on + strand, within Psyr_2957 at 3545.164 kb on - strand, within Psyr_2957 at 3545.164 kb on - strand, within Psyr_2957 at 3545.209 kb on + strand at 3545.246 kb on + strand at 3545.247 kb on - strand at 3545.317 kb on + strand at 3545.317 kb on + strand at 3545.317 kb on + strand at 3545.317 kb on + strand at 3545.317 kb on + strand at 3545.317 kb on + strand at 3545.317 kb on + strand at 3545.317 kb on + strand at 3545.318 kb on - strand at 3545.318 kb on - strand at 3545.318 kb on - strand at 3545.814 kb on + strand, within Psyr_2958 at 3545.909 kb on - strand, within Psyr_2958 at 3545.909 kb on - strand, within Psyr_2958 at 3545.983 kb on + strand, within Psyr_2958 at 3546.235 kb on + strand, within Psyr_2958 at 3546.235 kb on + strand, within Psyr_2958 at 3546.235 kb on + strand, within Psyr_2958 at 3546.238 kb on - strand, within Psyr_2958 at 3546.278 kb on - strand, within Psyr_2958 at 3546.343 kb on + strand, within Psyr_2958 at 3546.343 kb on + strand, within Psyr_2958 at 3546.344 kb on - strand, within Psyr_2958 at 3546.344 kb on - strand, within Psyr_2958 at 3546.344 kb on - strand, within Psyr_2958 at 3546.540 kb on + strand, within Psyr_2958 at 3546.584 kb on - strand, within Psyr_2958 at 3546.584 kb on - strand, within Psyr_2958 at 3546.584 kb on - strand, within Psyr_2958 at 3546.599 kb on - strand, within Psyr_2958 at 3546.599 kb on - strand, within Psyr_2958 at 3546.642 kb on + strand, within Psyr_2958 at 3546.643 kb on - strand, within Psyr_2958 at 3546.643 kb on - strand, within Psyr_2958 at 3546.643 kb on - strand, within Psyr_2958 at 3546.778 kb on + strand, within Psyr_2958 at 3546.779 kb on - strand, within Psyr_2958 at 3546.779 kb on - strand, within Psyr_2958 at 3546.779 kb on - strand, within Psyr_2958 at 3546.812 kb on + strand, within Psyr_2958 at 3546.812 kb on + strand, within Psyr_2958 at 3546.812 kb on + strand, within Psyr_2958 at 3546.812 kb on + strand, within Psyr_2958 at 3546.812 kb on + strand, within Psyr_2958 at 3546.812 kb on + strand, within Psyr_2958 at 3546.812 kb on + strand, within Psyr_2958 at 3546.813 kb on - strand, within Psyr_2958 at 3546.907 kb on + strand, within Psyr_2958 at 3546.907 kb on + strand, within Psyr_2958 at 3546.907 kb on + strand, within Psyr_2958 at 3546.907 kb on + strand, within Psyr_2958 at 3546.908 kb on - strand, within Psyr_2958 at 3546.908 kb on - strand, within Psyr_2958 at 3546.919 kb on + strand, within Psyr_2958 at 3546.919 kb on + strand, within Psyr_2958 at 3546.920 kb on - strand, within Psyr_2958 at 3546.920 kb on - strand, within Psyr_2958 at 3546.920 kb on - strand, within Psyr_2958 at 3546.920 kb on - strand, within Psyr_2958 at 3546.920 kb on - strand, within Psyr_2958 at 3546.920 kb on - strand, within Psyr_2958 at 3546.920 kb on - strand, within Psyr_2958 at 3546.920 kb on - strand, within Psyr_2958 at 3546.925 kb on + strand, within Psyr_2958 at 3546.925 kb on + strand, within Psyr_2958 at 3546.925 kb on + strand, within Psyr_2958 at 3546.925 kb on + strand, within Psyr_2958 at 3546.925 kb on + strand, within Psyr_2958 at 3546.926 kb on - strand, within Psyr_2958 at 3546.926 kb on - strand, within Psyr_2958 at 3546.926 kb on - strand, within Psyr_2958 at 3546.926 kb on - strand, within Psyr_2958 at 3546.926 kb on - strand, within Psyr_2958 at 3546.926 kb on - strand, within Psyr_2958 at 3546.926 kb on - strand, within Psyr_2958 at 3546.926 kb on - strand, within Psyr_2958 at 3546.926 kb on - strand, within Psyr_2958 at 3546.926 kb on - strand, within Psyr_2958 at 3546.926 kb on - strand, within Psyr_2958 at 3547.182 kb on - strand at 3547.255 kb on - strand at 3547.257 kb on - strand at 3547.288 kb on + strand at 3547.314 kb on + strand at 3547.314 kb on + strand at 3547.314 kb on + strand at 3547.314 kb on + strand at 3547.314 kb on + strand at 3547.315 kb on - strand at 3547.315 kb on - strand at 3547.315 kb on - strand at 3547.326 kb on + strand at 3547.422 kb on + strand, within Psyr_2959 at 3547.423 kb on - strand, within Psyr_2959 at 3547.423 kb on - strand, within Psyr_2959 at 3547.455 kb on + strand, within Psyr_2959 at 3547.455 kb on + strand, within Psyr_2959 at 3547.627 kb on - strand, within Psyr_2959 at 3547.627 kb on - strand, within Psyr_2959 at 3547.630 kb on + strand, within Psyr_2959 at 3547.783 kb on + strand, within Psyr_2959 at 3547.824 kb on + strand, within Psyr_2959 at 3547.825 kb on - strand, within Psyr_2959 at 3547.825 kb on - strand, within Psyr_2959 at 3547.872 kb on + strand, within Psyr_2959 at 3547.872 kb on + strand, within Psyr_2959 at 3547.872 kb on + strand, within Psyr_2959 at 3547.872 kb on + strand, within Psyr_2959 at 3547.873 kb on - strand, within Psyr_2959 at 3547.873 kb on - strand, within Psyr_2959 at 3547.873 kb on - strand, within Psyr_2959 at 3548.084 kb on - strand, within Psyr_2959 at 3548.084 kb on - strand, within Psyr_2959 at 3548.206 kb on - strand at 3548.206 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,544,647 + -1.2 3,544,648 - -0.3 3,544,649 + +1.3 3,544,650 - -3.5 3,544,659 + +0.4 3,544,659 + +0.7 3,544,659 + -0.1 3,544,660 - -3.6 3,544,932 - Psyr_2957 0.50 +1.7 3,544,970 + Psyr_2957 0.56 -0.3 3,544,970 + Psyr_2957 0.56 +1.3 3,544,970 + Psyr_2957 0.56 +1.5 3,544,970 + Psyr_2957 0.56 +0.7 3,544,970 + Psyr_2957 0.56 +4.2 3,544,971 - Psyr_2957 0.56 -0.7 3,544,971 - Psyr_2957 0.56 +0.3 3,544,971 - Psyr_2957 0.56 -0.8 3,544,974 + Psyr_2957 0.57 +2.3 3,544,974 + Psyr_2957 0.57 +3.7 3,544,974 + Psyr_2957 0.57 +1.7 3,544,974 + Psyr_2957 0.57 -0.3 3,544,974 + Psyr_2957 0.57 +1.5 3,544,974 + Psyr_2957 0.57 +1.7 3,544,974 + Psyr_2957 0.57 +2.7 3,544,974 + Psyr_2957 0.57 +3.1 3,544,974 + Psyr_2957 0.57 -1.8 3,544,975 - Psyr_2957 0.57 -1.1 3,544,975 - Psyr_2957 0.57 +2.1 3,544,975 - Psyr_2957 0.57 +0.4 3,544,975 - Psyr_2957 0.57 -2.6 3,544,975 - Psyr_2957 0.57 +2.7 3,544,975 - Psyr_2957 0.57 -0.6 3,544,975 - Psyr_2957 0.57 -1.6 3,544,975 - Psyr_2957 0.57 -0.9 3,544,975 - Psyr_2957 0.57 +2.6 3,544,975 - Psyr_2957 0.57 -0.4 3,544,975 - Psyr_2957 0.57 +2.7 3,544,975 - Psyr_2957 0.57 -1.3 3,545,163 + Psyr_2957 0.87 +2.9 3,545,164 - Psyr_2957 0.87 -0.1 3,545,164 - Psyr_2957 0.87 +2.1 3,545,209 + -0.1 3,545,246 + +0.1 3,545,247 - +0.7 3,545,317 + -1.0 3,545,317 + -0.7 3,545,317 + +3.4 3,545,317 + +0.0 3,545,317 + +4.6 3,545,317 + -2.8 3,545,317 + +0.7 3,545,317 + +2.6 3,545,318 - -4.1 3,545,318 - +1.7 3,545,318 - +2.3 3,545,814 + Psyr_2958 0.12 -2.5 3,545,909 - Psyr_2958 0.18 +4.0 3,545,909 - Psyr_2958 0.18 +0.1 3,545,983 + Psyr_2958 0.22 -1.5 3,546,235 + Psyr_2958 0.38 +2.3 3,546,235 + Psyr_2958 0.38 -2.5 3,546,235 + Psyr_2958 0.38 +3.6 3,546,238 - Psyr_2958 0.38 -1.4 3,546,278 - Psyr_2958 0.41 -1.4 3,546,343 + Psyr_2958 0.44 +3.1 3,546,343 + Psyr_2958 0.44 -1.3 3,546,344 - Psyr_2958 0.45 +2.1 3,546,344 - Psyr_2958 0.45 +2.1 3,546,344 - Psyr_2958 0.45 -0.8 3,546,540 + Psyr_2958 0.57 -1.0 3,546,584 - Psyr_2958 0.59 +3.7 3,546,584 - Psyr_2958 0.59 -2.1 3,546,584 - Psyr_2958 0.59 +0.6 3,546,599 - Psyr_2958 0.60 +2.6 3,546,599 - Psyr_2958 0.60 +0.5 3,546,642 + Psyr_2958 0.63 +1.4 3,546,643 - Psyr_2958 0.63 -0.8 3,546,643 - Psyr_2958 0.63 +0.7 3,546,643 - Psyr_2958 0.63 -0.3 3,546,778 + Psyr_2958 0.71 +0.7 3,546,779 - Psyr_2958 0.71 -0.3 3,546,779 - Psyr_2958 0.71 +3.7 3,546,779 - Psyr_2958 0.71 +1.6 3,546,812 + Psyr_2958 0.73 +3.2 3,546,812 + Psyr_2958 0.73 -1.4 3,546,812 + Psyr_2958 0.73 +2.1 3,546,812 + Psyr_2958 0.73 -1.0 3,546,812 + Psyr_2958 0.73 +1.3 3,546,812 + Psyr_2958 0.73 +3.6 3,546,812 + Psyr_2958 0.73 +1.7 3,546,813 - Psyr_2958 0.73 +2.3 3,546,907 + Psyr_2958 0.79 +2.9 3,546,907 + Psyr_2958 0.79 +2.7 3,546,907 + Psyr_2958 0.79 -1.3 3,546,907 + Psyr_2958 0.79 -0.9 3,546,908 - Psyr_2958 0.79 +2.7 3,546,908 - Psyr_2958 0.79 +2.1 3,546,919 + Psyr_2958 0.80 +0.7 3,546,919 + Psyr_2958 0.80 -2.7 3,546,920 - Psyr_2958 0.80 +0.6 3,546,920 - Psyr_2958 0.80 +1.3 3,546,920 - Psyr_2958 0.80 -1.8 3,546,920 - Psyr_2958 0.80 +0.1 3,546,920 - Psyr_2958 0.80 +1.7 3,546,920 - Psyr_2958 0.80 +3.2 3,546,920 - Psyr_2958 0.80 +0.1 3,546,920 - Psyr_2958 0.80 +2.3 3,546,925 + Psyr_2958 0.80 -0.5 3,546,925 + Psyr_2958 0.80 +0.9 3,546,925 + Psyr_2958 0.80 -1.6 3,546,925 + Psyr_2958 0.80 +1.7 3,546,925 + Psyr_2958 0.80 -1.5 3,546,926 - Psyr_2958 0.80 +4.1 3,546,926 - Psyr_2958 0.80 +0.2 3,546,926 - Psyr_2958 0.80 +2.9 3,546,926 - Psyr_2958 0.80 -3.8 3,546,926 - Psyr_2958 0.80 +1.7 3,546,926 - Psyr_2958 0.80 -1.5 3,546,926 - Psyr_2958 0.80 -1.9 3,546,926 - Psyr_2958 0.80 -1.4 3,546,926 - Psyr_2958 0.80 +2.3 3,546,926 - Psyr_2958 0.80 +2.3 3,546,926 - Psyr_2958 0.80 +2.3 3,547,182 - +3.7 3,547,255 - -2.1 3,547,257 - -1.5 3,547,288 + -1.4 3,547,314 + -2.5 3,547,314 + -1.4 3,547,314 + -1.1 3,547,314 + +0.7 3,547,314 + -0.3 3,547,315 - +3.1 3,547,315 - +1.3 3,547,315 - -1.2 3,547,326 + -0.4 3,547,422 + Psyr_2959 0.19 +0.7 3,547,423 - Psyr_2959 0.19 -0.7 3,547,423 - Psyr_2959 0.19 -1.4 3,547,455 + Psyr_2959 0.22 -1.9 3,547,455 + Psyr_2959 0.22 +3.3 3,547,627 - Psyr_2959 0.40 -2.9 3,547,627 - Psyr_2959 0.40 -1.3 3,547,630 + Psyr_2959 0.40 +4.1 3,547,783 + Psyr_2959 0.57 -1.5 3,547,824 + Psyr_2959 0.61 -3.3 3,547,825 - Psyr_2959 0.61 +1.3 3,547,825 - Psyr_2959 0.61 -1.7 3,547,872 + Psyr_2959 0.66 +3.3 3,547,872 + Psyr_2959 0.66 -0.4 3,547,872 + Psyr_2959 0.66 +2.1 3,547,872 + Psyr_2959 0.66 -0.3 3,547,873 - Psyr_2959 0.66 -0.3 3,547,873 - Psyr_2959 0.66 -1.6 3,547,873 - Psyr_2959 0.66 +2.5 3,548,084 - Psyr_2959 0.88 +4.4 3,548,084 - Psyr_2959 0.88 +3.6 3,548,206 - -0.1 3,548,206 - -1.0
Or see this region's nucleotide sequence