Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2942 and Psyr_2943 overlap by 4 nucleotides Psyr_2943 and Psyr_2944 overlap by 20 nucleotides Psyr_2944 and Psyr_2945 are separated by 307 nucleotides
Psyr_2942: Psyr_2942 - amino acid ABC transporter ATP-binding protein, PAAT family, at 3,533,112 to 3,533,873
_2942
Psyr_2943: Psyr_2943 - amino acid ABC transporter membrane protein 2, PAAT family, at 3,533,870 to 3,534,529
_2943
Psyr_2944: Psyr_2944 - amino acid ABC transporter membrane protein 1, PAAT family, at 3,534,510 to 3,535,175
_2944
Psyr_2945: Psyr_2945 - transcriptional regulator, LysR family, at 3,535,483 to 3,536,385
_2945
Position (kb)
3534
3535
3536 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 3533.586 kb on + strand, within Psyr_2942 at 3533.611 kb on + strand, within Psyr_2942 at 3533.611 kb on + strand, within Psyr_2942 at 3533.611 kb on + strand, within Psyr_2942 at 3533.612 kb on - strand, within Psyr_2942 at 3533.612 kb on - strand, within Psyr_2942 at 3533.612 kb on - strand, within Psyr_2942 at 3533.932 kb on + strand at 3533.958 kb on + strand, within Psyr_2943 at 3533.959 kb on - strand, within Psyr_2943 at 3533.977 kb on - strand, within Psyr_2943 at 3534.067 kb on - strand, within Psyr_2943 at 3534.067 kb on - strand, within Psyr_2943 at 3534.067 kb on - strand, within Psyr_2943 at 3534.067 kb on - strand, within Psyr_2943 at 3534.114 kb on - strand, within Psyr_2943 at 3534.126 kb on + strand, within Psyr_2943 at 3534.126 kb on + strand, within Psyr_2943 at 3534.127 kb on - strand, within Psyr_2943 at 3534.127 kb on - strand, within Psyr_2943 at 3534.127 kb on - strand, within Psyr_2943 at 3534.127 kb on - strand, within Psyr_2943 at 3534.312 kb on + strand, within Psyr_2943 at 3534.312 kb on + strand, within Psyr_2943 at 3534.313 kb on - strand, within Psyr_2943 at 3534.354 kb on + strand, within Psyr_2943 at 3534.424 kb on + strand, within Psyr_2943 at 3534.619 kb on + strand, within Psyr_2944 at 3534.629 kb on - strand, within Psyr_2944 at 3534.631 kb on + strand, within Psyr_2944 at 3534.632 kb on - strand, within Psyr_2944 at 3534.632 kb on - strand, within Psyr_2944 at 3534.643 kb on + strand, within Psyr_2944 at 3534.643 kb on + strand, within Psyr_2944 at 3534.643 kb on + strand, within Psyr_2944 at 3534.643 kb on + strand, within Psyr_2944 at 3534.643 kb on + strand, within Psyr_2944 at 3534.643 kb on + strand, within Psyr_2944 at 3534.643 kb on + strand, within Psyr_2944 at 3534.644 kb on - strand, within Psyr_2944 at 3534.644 kb on - strand, within Psyr_2944 at 3534.644 kb on - strand, within Psyr_2944 at 3534.644 kb on - strand, within Psyr_2944 at 3534.659 kb on - strand, within Psyr_2944 at 3534.843 kb on + strand, within Psyr_2944 at 3534.844 kb on - strand, within Psyr_2944 at 3534.872 kb on - strand, within Psyr_2944 at 3534.952 kb on + strand, within Psyr_2944 at 3534.952 kb on + strand, within Psyr_2944 at 3534.952 kb on + strand, within Psyr_2944 at 3534.953 kb on - strand, within Psyr_2944 at 3535.004 kb on - strand, within Psyr_2944 at 3535.004 kb on - strand, within Psyr_2944 at 3535.004 kb on - strand, within Psyr_2944 at 3535.043 kb on + strand, within Psyr_2944 at 3535.043 kb on + strand, within Psyr_2944 at 3535.044 kb on - strand, within Psyr_2944 at 3535.044 kb on - strand, within Psyr_2944 at 3535.044 kb on - strand, within Psyr_2944 at 3535.121 kb on + strand at 3535.235 kb on + strand at 3535.235 kb on + strand at 3535.235 kb on + strand at 3535.236 kb on - strand at 3535.236 kb on - strand at 3535.236 kb on - strand at 3535.236 kb on - strand at 3535.236 kb on - strand at 3535.236 kb on - strand at 3535.236 kb on - strand at 3535.236 kb on - strand at 3535.239 kb on + strand at 3535.332 kb on + strand at 3535.333 kb on - strand at 3535.349 kb on + strand at 3535.380 kb on + strand at 3535.380 kb on + strand at 3535.381 kb on - strand at 3535.526 kb on + strand at 3535.526 kb on + strand at 3535.526 kb on + strand at 3535.526 kb on + strand at 3535.526 kb on + strand at 3535.526 kb on + strand at 3535.526 kb on + strand at 3535.527 kb on - strand at 3535.527 kb on - strand at 3535.527 kb on - strand at 3535.573 kb on + strand at 3535.573 kb on + strand at 3535.574 kb on - strand, within Psyr_2945 at 3535.574 kb on - strand, within Psyr_2945 at 3535.574 kb on - strand, within Psyr_2945 at 3535.574 kb on - strand, within Psyr_2945 at 3535.574 kb on - strand, within Psyr_2945 at 3535.574 kb on - strand, within Psyr_2945 at 3535.574 kb on - strand, within Psyr_2945 at 3535.596 kb on - strand, within Psyr_2945 at 3535.596 kb on - strand, within Psyr_2945 at 3535.844 kb on - strand, within Psyr_2945 at 3535.862 kb on + strand, within Psyr_2945 at 3535.882 kb on - strand, within Psyr_2945 at 3535.882 kb on - strand, within Psyr_2945 at 3536.004 kb on + strand, within Psyr_2945 at 3536.024 kb on + strand, within Psyr_2945 at 3536.044 kb on + strand, within Psyr_2945 at 3536.045 kb on - strand, within Psyr_2945 at 3536.084 kb on - strand, within Psyr_2945 at 3536.085 kb on + strand, within Psyr_2945 at 3536.149 kb on + strand, within Psyr_2945 at 3536.149 kb on + strand, within Psyr_2945 at 3536.149 kb on + strand, within Psyr_2945 at 3536.149 kb on + strand, within Psyr_2945 at 3536.149 kb on + strand, within Psyr_2945
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,533,586 + Psyr_2942 0.62 +3.9 3,533,611 + Psyr_2942 0.65 -1.3 3,533,611 + Psyr_2942 0.65 -1.5 3,533,611 + Psyr_2942 0.65 +1.0 3,533,612 - Psyr_2942 0.66 +3.5 3,533,612 - Psyr_2942 0.66 +4.1 3,533,612 - Psyr_2942 0.66 +1.3 3,533,932 + +3.2 3,533,958 + Psyr_2943 0.13 -3.4 3,533,959 - Psyr_2943 0.13 -1.6 3,533,977 - Psyr_2943 0.16 -0.6 3,534,067 - Psyr_2943 0.30 +1.3 3,534,067 - Psyr_2943 0.30 -2.5 3,534,067 - Psyr_2943 0.30 -1.0 3,534,067 - Psyr_2943 0.30 -2.2 3,534,114 - Psyr_2943 0.37 -1.6 3,534,126 + Psyr_2943 0.39 +1.3 3,534,126 + Psyr_2943 0.39 -3.2 3,534,127 - Psyr_2943 0.39 +2.9 3,534,127 - Psyr_2943 0.39 -2.6 3,534,127 - Psyr_2943 0.39 -2.7 3,534,127 - Psyr_2943 0.39 +0.8 3,534,312 + Psyr_2943 0.67 -0.2 3,534,312 + Psyr_2943 0.67 +1.8 3,534,313 - Psyr_2943 0.67 +4.0 3,534,354 + Psyr_2943 0.73 +3.1 3,534,424 + Psyr_2943 0.84 -0.4 3,534,619 + Psyr_2944 0.16 +1.3 3,534,629 - Psyr_2944 0.18 -1.8 3,534,631 + Psyr_2944 0.18 -1.5 3,534,632 - Psyr_2944 0.18 -0.5 3,534,632 - Psyr_2944 0.18 +1.8 3,534,643 + Psyr_2944 0.20 -4.8 3,534,643 + Psyr_2944 0.20 -0.9 3,534,643 + Psyr_2944 0.20 -1.7 3,534,643 + Psyr_2944 0.20 +0.8 3,534,643 + Psyr_2944 0.20 -0.2 3,534,643 + Psyr_2944 0.20 -0.2 3,534,643 + Psyr_2944 0.20 -1.8 3,534,644 - Psyr_2944 0.20 -0.2 3,534,644 - Psyr_2944 0.20 +3.2 3,534,644 - Psyr_2944 0.20 +3.2 3,534,644 - Psyr_2944 0.20 -2.8 3,534,659 - Psyr_2944 0.22 -2.4 3,534,843 + Psyr_2944 0.50 -0.5 3,534,844 - Psyr_2944 0.50 -0.8 3,534,872 - Psyr_2944 0.54 +0.1 3,534,952 + Psyr_2944 0.66 +0.8 3,534,952 + Psyr_2944 0.66 -2.4 3,534,952 + Psyr_2944 0.66 +2.1 3,534,953 - Psyr_2944 0.67 -0.1 3,535,004 - Psyr_2944 0.74 -1.3 3,535,004 - Psyr_2944 0.74 +1.2 3,535,004 - Psyr_2944 0.74 +2.1 3,535,043 + Psyr_2944 0.80 -0.2 3,535,043 + Psyr_2944 0.80 -1.1 3,535,044 - Psyr_2944 0.80 -1.1 3,535,044 - Psyr_2944 0.80 +3.7 3,535,044 - Psyr_2944 0.80 +2.8 3,535,121 + +0.4 3,535,235 + +2.6 3,535,235 + -0.6 3,535,235 + -1.4 3,535,236 - +1.8 3,535,236 - -0.2 3,535,236 - +0.8 3,535,236 - -0.8 3,535,236 - -2.4 3,535,236 - +0.8 3,535,236 - +1.3 3,535,236 - +2.8 3,535,239 + +2.8 3,535,332 + -1.6 3,535,333 - -0.6 3,535,349 + +3.1 3,535,380 + +2.3 3,535,380 + +3.5 3,535,381 - -2.2 3,535,526 + +3.2 3,535,526 + +3.3 3,535,526 + -1.7 3,535,526 + +0.1 3,535,526 + -1.5 3,535,526 + -0.5 3,535,526 + -4.4 3,535,527 - +3.8 3,535,527 - -1.5 3,535,527 - +0.3 3,535,573 + +1.8 3,535,573 + -2.3 3,535,574 - Psyr_2945 0.10 +0.1 3,535,574 - Psyr_2945 0.10 -1.6 3,535,574 - Psyr_2945 0.10 +2.6 3,535,574 - Psyr_2945 0.10 +2.3 3,535,574 - Psyr_2945 0.10 +0.3 3,535,574 - Psyr_2945 0.10 -0.7 3,535,574 - Psyr_2945 0.10 +3.2 3,535,596 - Psyr_2945 0.13 +3.7 3,535,596 - Psyr_2945 0.13 +2.9 3,535,844 - Psyr_2945 0.40 +2.9 3,535,862 + Psyr_2945 0.42 -1.5 3,535,882 - Psyr_2945 0.44 -0.9 3,535,882 - Psyr_2945 0.44 +2.8 3,536,004 + Psyr_2945 0.58 -2.2 3,536,024 + Psyr_2945 0.60 -2.6 3,536,044 + Psyr_2945 0.62 +1.8 3,536,045 - Psyr_2945 0.62 -0.5 3,536,084 - Psyr_2945 0.67 +3.5 3,536,085 + Psyr_2945 0.67 -1.5 3,536,149 + Psyr_2945 0.74 +2.8 3,536,149 + Psyr_2945 0.74 -1.2 3,536,149 + Psyr_2945 0.74 +0.8 3,536,149 + Psyr_2945 0.74 +3.1 3,536,149 + Psyr_2945 0.74 +4.1
Or see this region's nucleotide sequence