Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2888 and Psyr_2889 are separated by 22 nucleotides Psyr_2889 and Psyr_2890 are separated by 28 nucleotides Psyr_2890 and Psyr_2891 overlap by 8 nucleotides
Psyr_2888: Psyr_2888 - Amine oxidase, at 3,464,642 to 3,466,225
_2888
Psyr_2889: Psyr_2889 - amino acid ABC transporter substrate-binding protein, PAAT family, at 3,466,248 to 3,467,177
_2889
Psyr_2890: Psyr_2890 - amino acid ABC transporter ATP-binding protein, PAAT family, at 3,467,206 to 3,467,991
_2890
Psyr_2891: Psyr_2891 - amino acid ABC transporter membrane protein, PAAT family, at 3,467,984 to 3,468,973
_2891
Position (kb)
3466
3467
3468 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3465.256 kb on - strand, within Psyr_2888 at 3465.288 kb on + strand, within Psyr_2888 at 3465.288 kb on + strand, within Psyr_2888 at 3465.289 kb on - strand, within Psyr_2888 at 3465.289 kb on - strand, within Psyr_2888 at 3465.300 kb on + strand, within Psyr_2888 at 3465.300 kb on + strand, within Psyr_2888 at 3465.300 kb on + strand, within Psyr_2888 at 3465.301 kb on - strand, within Psyr_2888 at 3465.304 kb on + strand, within Psyr_2888 at 3465.305 kb on - strand, within Psyr_2888 at 3465.305 kb on - strand, within Psyr_2888 at 3465.305 kb on - strand, within Psyr_2888 at 3465.382 kb on - strand, within Psyr_2888 at 3465.606 kb on + strand, within Psyr_2888 at 3465.606 kb on + strand, within Psyr_2888 at 3465.768 kb on - strand, within Psyr_2888 at 3465.797 kb on + strand, within Psyr_2888 at 3465.797 kb on + strand, within Psyr_2888 at 3465.798 kb on - strand, within Psyr_2888 at 3465.882 kb on + strand, within Psyr_2888 at 3465.883 kb on - strand, within Psyr_2888 at 3465.883 kb on - strand, within Psyr_2888 at 3465.883 kb on - strand, within Psyr_2888 at 3465.883 kb on - strand, within Psyr_2888 at 3465.927 kb on + strand, within Psyr_2888 at 3465.927 kb on + strand, within Psyr_2888 at 3466.484 kb on - strand, within Psyr_2889 at 3466.489 kb on + strand, within Psyr_2889 at 3466.489 kb on + strand, within Psyr_2889 at 3466.489 kb on + strand, within Psyr_2889 at 3466.489 kb on + strand, within Psyr_2889 at 3466.489 kb on + strand, within Psyr_2889 at 3466.489 kb on + strand, within Psyr_2889 at 3466.489 kb on + strand, within Psyr_2889 at 3466.489 kb on + strand, within Psyr_2889 at 3466.489 kb on + strand, within Psyr_2889 at 3466.489 kb on + strand, within Psyr_2889 at 3466.489 kb on + strand, within Psyr_2889 at 3466.490 kb on - strand, within Psyr_2889 at 3466.490 kb on - strand, within Psyr_2889 at 3466.490 kb on - strand, within Psyr_2889 at 3466.490 kb on - strand, within Psyr_2889 at 3466.490 kb on - strand, within Psyr_2889 at 3466.490 kb on - strand, within Psyr_2889 at 3466.490 kb on - strand, within Psyr_2889 at 3466.490 kb on - strand, within Psyr_2889 at 3466.490 kb on - strand, within Psyr_2889 at 3466.490 kb on - strand, within Psyr_2889 at 3466.490 kb on - strand, within Psyr_2889 at 3466.490 kb on - strand, within Psyr_2889 at 3466.490 kb on - strand, within Psyr_2889 at 3466.490 kb on - strand, within Psyr_2889 at 3466.490 kb on - strand, within Psyr_2889 at 3466.492 kb on - strand, within Psyr_2889 at 3466.492 kb on - strand, within Psyr_2889 at 3466.492 kb on - strand, within Psyr_2889 at 3466.568 kb on - strand at 3466.693 kb on - strand, within Psyr_2889 at 3466.723 kb on + strand, within Psyr_2889 at 3466.723 kb on + strand, within Psyr_2889 at 3466.724 kb on - strand, within Psyr_2889 at 3466.724 kb on - strand, within Psyr_2889 at 3466.748 kb on - strand, within Psyr_2889 at 3467.117 kb on + strand at 3467.117 kb on + strand at 3467.117 kb on + strand at 3467.118 kb on - strand at 3467.118 kb on - strand at 3467.209 kb on + strand at 3467.209 kb on + strand at 3467.210 kb on - strand at 3467.735 kb on + strand, within Psyr_2890 at 3467.736 kb on - strand, within Psyr_2890 at 3467.769 kb on - strand, within Psyr_2890 at 3467.769 kb on - strand, within Psyr_2890 at 3467.769 kb on - strand, within Psyr_2890 at 3467.864 kb on - strand, within Psyr_2890 at 3468.059 kb on + strand at 3468.096 kb on + strand, within Psyr_2891 at 3468.096 kb on + strand, within Psyr_2891 at 3468.096 kb on + strand, within Psyr_2891 at 3468.096 kb on + strand, within Psyr_2891 at 3468.097 kb on - strand, within Psyr_2891 at 3468.097 kb on - strand, within Psyr_2891 at 3468.097 kb on - strand, within Psyr_2891 at 3468.121 kb on + strand, within Psyr_2891
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,465,256 - Psyr_2888 0.39 -2.2 3,465,288 + Psyr_2888 0.41 -0.8 3,465,288 + Psyr_2888 0.41 -0.3 3,465,289 - Psyr_2888 0.41 +2.3 3,465,289 - Psyr_2888 0.41 +2.1 3,465,300 + Psyr_2888 0.42 +2.1 3,465,300 + Psyr_2888 0.42 +3.8 3,465,300 + Psyr_2888 0.42 +3.7 3,465,301 - Psyr_2888 0.42 +3.1 3,465,304 + Psyr_2888 0.42 -2.4 3,465,305 - Psyr_2888 0.42 +0.8 3,465,305 - Psyr_2888 0.42 +0.4 3,465,305 - Psyr_2888 0.42 -1.1 3,465,382 - Psyr_2888 0.47 -2.6 3,465,606 + Psyr_2888 0.61 -4.1 3,465,606 + Psyr_2888 0.61 -1.6 3,465,768 - Psyr_2888 0.71 +1.8 3,465,797 + Psyr_2888 0.73 -1.0 3,465,797 + Psyr_2888 0.73 -2.3 3,465,798 - Psyr_2888 0.73 -1.9 3,465,882 + Psyr_2888 0.78 +0.8 3,465,883 - Psyr_2888 0.78 -0.0 3,465,883 - Psyr_2888 0.78 -1.8 3,465,883 - Psyr_2888 0.78 -0.5 3,465,883 - Psyr_2888 0.78 +2.3 3,465,927 + Psyr_2888 0.81 +1.6 3,465,927 + Psyr_2888 0.81 +1.3 3,466,484 - Psyr_2889 0.25 +4.6 3,466,489 + Psyr_2889 0.26 -0.2 3,466,489 + Psyr_2889 0.26 +0.8 3,466,489 + Psyr_2889 0.26 +0.8 3,466,489 + Psyr_2889 0.26 +1.5 3,466,489 + Psyr_2889 0.26 +2.6 3,466,489 + Psyr_2889 0.26 +0.8 3,466,489 + Psyr_2889 0.26 +0.8 3,466,489 + Psyr_2889 0.26 -0.2 3,466,489 + Psyr_2889 0.26 +2.9 3,466,489 + Psyr_2889 0.26 -1.3 3,466,489 + Psyr_2889 0.26 -0.6 3,466,490 - Psyr_2889 0.26 +2.8 3,466,490 - Psyr_2889 0.26 -1.8 3,466,490 - Psyr_2889 0.26 +3.8 3,466,490 - Psyr_2889 0.26 +0.5 3,466,490 - Psyr_2889 0.26 +1.1 3,466,490 - Psyr_2889 0.26 -0.5 3,466,490 - Psyr_2889 0.26 +2.8 3,466,490 - Psyr_2889 0.26 +0.8 3,466,490 - Psyr_2889 0.26 -1.6 3,466,490 - Psyr_2889 0.26 -1.2 3,466,490 - Psyr_2889 0.26 -3.0 3,466,490 - Psyr_2889 0.26 -1.1 3,466,490 - Psyr_2889 0.26 +3.1 3,466,490 - Psyr_2889 0.26 +3.8 3,466,490 - Psyr_2889 0.26 +2.9 3,466,492 - Psyr_2889 0.26 -0.6 3,466,492 - Psyr_2889 0.26 -0.8 3,466,492 - Psyr_2889 0.26 +3.3 3,466,568 - +1.3 3,466,693 - Psyr_2889 0.48 +1.3 3,466,723 + Psyr_2889 0.51 +2.6 3,466,723 + Psyr_2889 0.51 +3.7 3,466,724 - Psyr_2889 0.51 -2.3 3,466,724 - Psyr_2889 0.51 +2.6 3,466,748 - Psyr_2889 0.54 -2.3 3,467,117 + -2.6 3,467,117 + +1.8 3,467,117 + +3.1 3,467,118 - -0.6 3,467,118 - -1.2 3,467,209 + +2.3 3,467,209 + -0.2 3,467,210 - -1.9 3,467,735 + Psyr_2890 0.67 +2.6 3,467,736 - Psyr_2890 0.67 -1.8 3,467,769 - Psyr_2890 0.72 +0.0 3,467,769 - Psyr_2890 0.72 -4.1 3,467,769 - Psyr_2890 0.72 +3.1 3,467,864 - Psyr_2890 0.84 +2.3 3,468,059 + +3.3 3,468,096 + Psyr_2891 0.11 +3.3 3,468,096 + Psyr_2891 0.11 -0.0 3,468,096 + Psyr_2891 0.11 +2.3 3,468,096 + Psyr_2891 0.11 +3.7 3,468,097 - Psyr_2891 0.11 -1.2 3,468,097 - Psyr_2891 0.11 -1.9 3,468,097 - Psyr_2891 0.11 +3.6 3,468,121 + Psyr_2891 0.14 -1.2
Or see this region's nucleotide sequence