Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2885 and Psyr_2886 are separated by 0 nucleotides Psyr_2886 and Psyr_2887 are separated by 33 nucleotides Psyr_2887 and Psyr_2888 are separated by 69 nucleotides
Psyr_2885: Psyr_2885 - xylose ABC transporter ATP-binding protein, at 3,461,411 to 3,462,967
_2885
Psyr_2886: Psyr_2886 - xylose ABC transporter membrane protein, at 3,462,968 to 3,464,104
_2886
Psyr_2887: Psyr_2887 - Endoribonuclease L-PSP, at 3,464,138 to 3,464,572
_2887
Psyr_2888: Psyr_2888 - Amine oxidase, at 3,464,642 to 3,466,225
_2888
Position (kb)
3462
3463
3464
3465 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3462.008 kb on + strand, within Psyr_2885 at 3462.008 kb on + strand, within Psyr_2885 at 3462.008 kb on + strand, within Psyr_2885 at 3462.009 kb on - strand, within Psyr_2885 at 3462.009 kb on - strand, within Psyr_2885 at 3462.009 kb on - strand, within Psyr_2885 at 3462.009 kb on - strand, within Psyr_2885 at 3462.219 kb on - strand, within Psyr_2885 at 3462.741 kb on - strand, within Psyr_2885 at 3462.741 kb on - strand, within Psyr_2885 at 3462.749 kb on + strand, within Psyr_2885 at 3462.750 kb on - strand, within Psyr_2885 at 3462.750 kb on - strand, within Psyr_2885 at 3462.750 kb on - strand, within Psyr_2885 at 3462.946 kb on + strand at 3462.965 kb on + strand at 3463.232 kb on + strand, within Psyr_2886 at 3463.304 kb on + strand, within Psyr_2886 at 3463.304 kb on + strand, within Psyr_2886 at 3463.306 kb on + strand, within Psyr_2886 at 3463.306 kb on + strand, within Psyr_2886 at 3463.306 kb on + strand, within Psyr_2886 at 3463.306 kb on + strand, within Psyr_2886 at 3463.307 kb on - strand, within Psyr_2886 at 3463.430 kb on + strand, within Psyr_2886 at 3463.430 kb on + strand, within Psyr_2886 at 3463.431 kb on - strand, within Psyr_2886 at 3463.431 kb on - strand, within Psyr_2886 at 3463.431 kb on - strand, within Psyr_2886 at 3463.431 kb on - strand, within Psyr_2886 at 3463.616 kb on + strand, within Psyr_2886 at 3463.616 kb on + strand, within Psyr_2886 at 3463.616 kb on + strand, within Psyr_2886 at 3463.617 kb on - strand, within Psyr_2886 at 3463.617 kb on - strand, within Psyr_2886 at 3463.617 kb on - strand, within Psyr_2886 at 3463.631 kb on + strand, within Psyr_2886 at 3463.632 kb on - strand, within Psyr_2886 at 3463.688 kb on + strand, within Psyr_2886 at 3463.688 kb on + strand, within Psyr_2886 at 3463.689 kb on - strand, within Psyr_2886 at 3463.689 kb on - strand, within Psyr_2886 at 3463.707 kb on + strand, within Psyr_2886 at 3463.773 kb on + strand, within Psyr_2886 at 3463.773 kb on + strand, within Psyr_2886 at 3463.774 kb on - strand, within Psyr_2886 at 3463.788 kb on + strand, within Psyr_2886 at 3463.840 kb on + strand, within Psyr_2886 at 3463.981 kb on + strand, within Psyr_2886 at 3464.083 kb on + strand at 3464.336 kb on + strand, within Psyr_2887 at 3464.336 kb on + strand, within Psyr_2887 at 3464.489 kb on + strand, within Psyr_2887 at 3464.603 kb on + strand at 3464.604 kb on - strand at 3464.604 kb on - strand at 3464.611 kb on - strand at 3464.611 kb on - strand at 3464.614 kb on + strand at 3464.614 kb on + strand at 3464.781 kb on + strand at 3464.792 kb on - strand at 3464.862 kb on + strand, within Psyr_2888 at 3464.955 kb on + strand, within Psyr_2888 at 3464.955 kb on + strand, within Psyr_2888 at 3464.956 kb on - strand, within Psyr_2888 at 3465.009 kb on + strand, within Psyr_2888 at 3465.010 kb on - strand, within Psyr_2888 at 3465.010 kb on - strand, within Psyr_2888 at 3465.103 kb on - strand, within Psyr_2888
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,462,008 + Psyr_2885 0.38 +1.4 3,462,008 + Psyr_2885 0.38 -1.7 3,462,008 + Psyr_2885 0.38 +1.8 3,462,009 - Psyr_2885 0.38 +2.6 3,462,009 - Psyr_2885 0.38 +1.8 3,462,009 - Psyr_2885 0.38 +3.8 3,462,009 - Psyr_2885 0.38 +1.1 3,462,219 - Psyr_2885 0.52 +0.8 3,462,741 - Psyr_2885 0.85 +2.1 3,462,741 - Psyr_2885 0.85 +3.5 3,462,749 + Psyr_2885 0.86 -0.9 3,462,750 - Psyr_2885 0.86 -0.5 3,462,750 - Psyr_2885 0.86 -1.1 3,462,750 - Psyr_2885 0.86 +1.8 3,462,946 + -2.0 3,462,965 + -0.2 3,463,232 + Psyr_2886 0.23 +4.4 3,463,304 + Psyr_2886 0.30 -1.8 3,463,304 + Psyr_2886 0.30 +0.0 3,463,306 + Psyr_2886 0.30 +2.1 3,463,306 + Psyr_2886 0.30 -2.1 3,463,306 + Psyr_2886 0.30 +3.4 3,463,306 + Psyr_2886 0.30 -1.1 3,463,307 - Psyr_2886 0.30 +2.8 3,463,430 + Psyr_2886 0.41 +4.0 3,463,430 + Psyr_2886 0.41 -0.7 3,463,431 - Psyr_2886 0.41 -2.3 3,463,431 - Psyr_2886 0.41 +2.8 3,463,431 - Psyr_2886 0.41 -2.1 3,463,431 - Psyr_2886 0.41 -2.0 3,463,616 + Psyr_2886 0.57 +4.5 3,463,616 + Psyr_2886 0.57 +3.1 3,463,616 + Psyr_2886 0.57 -0.4 3,463,617 - Psyr_2886 0.57 +3.0 3,463,617 - Psyr_2886 0.57 +2.1 3,463,617 - Psyr_2886 0.57 -2.2 3,463,631 + Psyr_2886 0.58 -0.3 3,463,632 - Psyr_2886 0.58 +1.2 3,463,688 + Psyr_2886 0.63 -3.1 3,463,688 + Psyr_2886 0.63 +4.0 3,463,689 - Psyr_2886 0.63 -0.2 3,463,689 - Psyr_2886 0.63 -0.8 3,463,707 + Psyr_2886 0.65 +1.0 3,463,773 + Psyr_2886 0.71 +2.1 3,463,773 + Psyr_2886 0.71 +3.0 3,463,774 - Psyr_2886 0.71 +0.8 3,463,788 + Psyr_2886 0.72 +1.8 3,463,840 + Psyr_2886 0.77 +3.4 3,463,981 + Psyr_2886 0.89 +3.1 3,464,083 + -1.7 3,464,336 + Psyr_2887 0.46 -0.4 3,464,336 + Psyr_2887 0.46 -0.2 3,464,489 + Psyr_2887 0.81 +0.9 3,464,603 + -2.4 3,464,604 - -2.5 3,464,604 - +0.3 3,464,611 - -1.7 3,464,611 - +3.6 3,464,614 + +2.9 3,464,614 + -1.0 3,464,781 + -0.1 3,464,792 - -0.2 3,464,862 + Psyr_2888 0.14 +1.8 3,464,955 + Psyr_2888 0.20 -1.5 3,464,955 + Psyr_2888 0.20 +0.2 3,464,956 - Psyr_2888 0.20 +1.8 3,465,009 + Psyr_2888 0.23 -1.4 3,465,010 - Psyr_2888 0.23 -3.2 3,465,010 - Psyr_2888 0.23 +1.3 3,465,103 - Psyr_2888 0.29 -1.9
Or see this region's nucleotide sequence