Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2871 and Psyr_2872 are separated by 38 nucleotides Psyr_2872 and Psyr_2873 are separated by 148 nucleotides
Psyr_2871: Psyr_2871 - CDS, at 3,445,461 to 3,446,675
_2871
Psyr_2872: Psyr_2872 - hypothetical protein, at 3,446,714 to 3,446,953
_2872
Psyr_2873: Psyr_2873 - Acyl-CoA dehydrogenase, C-terminal, at 3,447,102 to 3,448,340
_2873
Position (kb)
3446
3447 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3445.812 kb on - strand, within Psyr_2871 at 3445.812 kb on - strand, within Psyr_2871 at 3446.007 kb on + strand, within Psyr_2871 at 3446.023 kb on - strand, within Psyr_2871 at 3446.073 kb on - strand, within Psyr_2871 at 3446.186 kb on - strand, within Psyr_2871 at 3446.317 kb on + strand, within Psyr_2871 at 3446.317 kb on + strand, within Psyr_2871 at 3446.446 kb on + strand, within Psyr_2871 at 3446.446 kb on + strand, within Psyr_2871 at 3446.446 kb on + strand, within Psyr_2871 at 3446.446 kb on + strand, within Psyr_2871 at 3446.446 kb on + strand, within Psyr_2871 at 3446.447 kb on - strand, within Psyr_2871 at 3446.447 kb on - strand, within Psyr_2871 at 3446.447 kb on - strand, within Psyr_2871 at 3446.484 kb on - strand, within Psyr_2871 at 3446.888 kb on + strand, within Psyr_2872 at 3446.888 kb on + strand, within Psyr_2872 at 3446.889 kb on - strand, within Psyr_2872 at 3447.096 kb on + strand at 3447.573 kb on + strand, within Psyr_2873 at 3447.576 kb on + strand, within Psyr_2873 at 3447.577 kb on - strand, within Psyr_2873 at 3447.577 kb on - strand, within Psyr_2873 at 3447.577 kb on - strand, within Psyr_2873 at 3447.615 kb on + strand, within Psyr_2873 at 3447.616 kb on - strand, within Psyr_2873 at 3447.616 kb on - strand, within Psyr_2873 at 3447.616 kb on - strand, within Psyr_2873 at 3447.799 kb on - strand, within Psyr_2873
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,445,812 - Psyr_2871 0.29 +3.0 3,445,812 - Psyr_2871 0.29 +1.4 3,446,007 + Psyr_2871 0.45 +0.4 3,446,023 - Psyr_2871 0.46 -0.1 3,446,073 - Psyr_2871 0.50 -0.4 3,446,186 - Psyr_2871 0.60 +1.4 3,446,317 + Psyr_2871 0.70 -1.1 3,446,317 + Psyr_2871 0.70 -0.8 3,446,446 + Psyr_2871 0.81 +0.5 3,446,446 + Psyr_2871 0.81 +3.5 3,446,446 + Psyr_2871 0.81 +3.5 3,446,446 + Psyr_2871 0.81 -0.2 3,446,446 + Psyr_2871 0.81 -0.7 3,446,447 - Psyr_2871 0.81 -4.2 3,446,447 - Psyr_2871 0.81 +0.8 3,446,447 - Psyr_2871 0.81 +3.4 3,446,484 - Psyr_2871 0.84 +2.5 3,446,888 + Psyr_2872 0.72 -1.1 3,446,888 + Psyr_2872 0.72 -4.4 3,446,889 - Psyr_2872 0.73 -3.2 3,447,096 + -2.1 3,447,573 + Psyr_2873 0.38 -2.3 3,447,576 + Psyr_2873 0.38 +0.8 3,447,577 - Psyr_2873 0.38 +3.0 3,447,577 - Psyr_2873 0.38 +2.6 3,447,577 - Psyr_2873 0.38 -1.8 3,447,615 + Psyr_2873 0.41 +0.4 3,447,616 - Psyr_2873 0.41 +1.8 3,447,616 - Psyr_2873 0.41 -0.2 3,447,616 - Psyr_2873 0.41 +3.2 3,447,799 - Psyr_2873 0.56 -1.0
Or see this region's nucleotide sequence