Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2865 and Psyr_2866 are separated by 2 nucleotides Psyr_2866 and Psyr_2867 are separated by 144 nucleotides
Psyr_2865: Psyr_2865 - Hydrophobe/amphiphile efflux-1 HAE1, at 3,436,901 to 3,440,047
_2865
Psyr_2866: Psyr_2866 - Secretion protein HlyD, at 3,440,050 to 3,441,252
_2866
Psyr_2867: Psyr_2867 - ATP-binding region, ATPase-like:Histidine kinase, HAMP region:Histidine kinase A, N-terminal, at 3,441,397 to 3,442,788
_2867
Position (kb)
3440
3441
3442 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3439.224 kb on + strand, within Psyr_2865 at 3439.492 kb on + strand, within Psyr_2865 at 3439.493 kb on - strand, within Psyr_2865 at 3439.578 kb on + strand, within Psyr_2865 at 3439.579 kb on - strand, within Psyr_2865 at 3439.636 kb on + strand, within Psyr_2865 at 3439.636 kb on + strand, within Psyr_2865 at 3439.818 kb on + strand at 3439.818 kb on + strand at 3439.818 kb on + strand at 3439.818 kb on + strand at 3439.818 kb on + strand at 3439.818 kb on + strand at 3439.818 kb on + strand at 3439.818 kb on + strand at 3439.818 kb on + strand at 3439.818 kb on + strand at 3439.818 kb on + strand at 3439.818 kb on + strand at 3439.818 kb on + strand at 3439.818 kb on + strand at 3439.819 kb on - strand at 3439.819 kb on - strand at 3439.819 kb on - strand at 3439.819 kb on - strand at 3439.819 kb on - strand at 3439.819 kb on - strand at 3439.819 kb on - strand at 3439.819 kb on - strand at 3439.902 kb on + strand at 3439.902 kb on + strand at 3439.903 kb on - strand at 3440.369 kb on + strand, within Psyr_2866 at 3440.369 kb on + strand, within Psyr_2866 at 3440.369 kb on + strand, within Psyr_2866 at 3440.370 kb on - strand, within Psyr_2866 at 3440.370 kb on - strand, within Psyr_2866 at 3440.370 kb on - strand, within Psyr_2866 at 3440.370 kb on - strand, within Psyr_2866 at 3440.370 kb on - strand, within Psyr_2866 at 3440.370 kb on - strand, within Psyr_2866 at 3440.370 kb on - strand, within Psyr_2866 at 3440.483 kb on + strand, within Psyr_2866 at 3440.483 kb on + strand, within Psyr_2866 at 3440.484 kb on - strand, within Psyr_2866 at 3440.609 kb on + strand, within Psyr_2866 at 3440.945 kb on + strand, within Psyr_2866 at 3440.945 kb on + strand, within Psyr_2866 at 3440.946 kb on - strand, within Psyr_2866 at 3440.967 kb on - strand, within Psyr_2866 at 3441.044 kb on - strand, within Psyr_2866 at 3441.200 kb on + strand at 3441.200 kb on + strand at 3441.200 kb on + strand at 3441.200 kb on + strand at 3441.200 kb on + strand at 3441.201 kb on - strand at 3441.201 kb on - strand at 3441.201 kb on - strand at 3441.227 kb on + strand at 3441.227 kb on + strand at 3441.228 kb on - strand at 3441.361 kb on - strand at 3441.571 kb on + strand, within Psyr_2867 at 3441.685 kb on - strand, within Psyr_2867 at 3441.877 kb on + strand, within Psyr_2867
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,439,224 + Psyr_2865 0.74 +1.4 3,439,492 + Psyr_2865 0.82 +3.6 3,439,493 - Psyr_2865 0.82 +1.2 3,439,578 + Psyr_2865 0.85 +2.1 3,439,579 - Psyr_2865 0.85 -3.3 3,439,636 + Psyr_2865 0.87 -0.1 3,439,636 + Psyr_2865 0.87 +3.8 3,439,818 + -0.2 3,439,818 + +4.0 3,439,818 + +2.1 3,439,818 + +2.9 3,439,818 + +2.4 3,439,818 + -2.7 3,439,818 + -2.2 3,439,818 + +1.8 3,439,818 + +4.4 3,439,818 + +3.2 3,439,818 + -1.0 3,439,818 + +3.1 3,439,818 + +3.4 3,439,818 + +0.8 3,439,819 - +2.1 3,439,819 - +2.6 3,439,819 - +3.2 3,439,819 - +2.6 3,439,819 - -2.5 3,439,819 - -2.1 3,439,819 - +3.4 3,439,819 - -0.2 3,439,902 + +1.0 3,439,902 + -3.0 3,439,903 - +3.7 3,440,369 + Psyr_2866 0.27 -0.4 3,440,369 + Psyr_2866 0.27 +2.8 3,440,369 + Psyr_2866 0.27 -2.6 3,440,370 - Psyr_2866 0.27 -0.5 3,440,370 - Psyr_2866 0.27 -1.5 3,440,370 - Psyr_2866 0.27 -2.2 3,440,370 - Psyr_2866 0.27 -1.9 3,440,370 - Psyr_2866 0.27 +2.6 3,440,370 - Psyr_2866 0.27 -3.3 3,440,370 - Psyr_2866 0.27 +2.1 3,440,483 + Psyr_2866 0.36 -0.4 3,440,483 + Psyr_2866 0.36 -2.4 3,440,484 - Psyr_2866 0.36 -1.5 3,440,609 + Psyr_2866 0.46 -3.2 3,440,945 + Psyr_2866 0.74 -2.3 3,440,945 + Psyr_2866 0.74 +2.1 3,440,946 - Psyr_2866 0.74 -0.6 3,440,967 - Psyr_2866 0.76 -2.5 3,441,044 - Psyr_2866 0.83 +3.6 3,441,200 + -0.0 3,441,200 + -0.3 3,441,200 + -1.1 3,441,200 + -1.1 3,441,200 + -1.2 3,441,201 - +2.9 3,441,201 - +0.8 3,441,201 - +1.0 3,441,227 + -2.2 3,441,227 + +2.4 3,441,228 - -0.8 3,441,361 - -2.3 3,441,571 + Psyr_2867 0.12 +3.0 3,441,685 - Psyr_2867 0.21 +2.1 3,441,877 + Psyr_2867 0.34 -2.9
Or see this region's nucleotide sequence