Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2860 and Psyr_2861 are separated by 100 nucleotides Psyr_2861 and Psyr_2862 are separated by 314 nucleotides Psyr_2862 and Psyr_RNA44 are separated by 156 nucleotides Psyr_RNA44 and Psyr_2863 are separated by 200 nucleotides
Psyr_2860: Psyr_2860 - hypothetical protein, at 3,431,317 to 3,431,694
_2860
Psyr_2861: Psyr_2861 - Hypothetical protein, at 3,431,795 to 3,432,085
_2861
Psyr_2862: Psyr_2862 - Cytochrome c, class I, at 3,432,400 to 3,433,206
_2862
Psyr_RNA44: Psyr_RNA44 - tRNA-Cys, at 3,433,363 to 3,433,436
_RNA44
Psyr_2863: Psyr_2863 - Feruloyl esterase, at 3,433,637 to 3,435,376
_2863
Position (kb)
3432
3433
3434 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3431.430 kb on + strand, within Psyr_2860 at 3431.431 kb on - strand, within Psyr_2860 at 3431.591 kb on + strand, within Psyr_2860 at 3431.647 kb on + strand, within Psyr_2860 at 3431.668 kb on + strand at 3431.668 kb on + strand at 3431.668 kb on + strand at 3431.669 kb on - strand at 3431.669 kb on - strand at 3431.688 kb on + strand at 3431.689 kb on - strand at 3431.750 kb on - strand at 3431.750 kb on - strand at 3432.137 kb on - strand at 3432.147 kb on + strand at 3432.147 kb on + strand at 3432.148 kb on - strand at 3432.148 kb on - strand at 3432.148 kb on - strand at 3432.153 kb on + strand at 3432.153 kb on + strand at 3432.156 kb on + strand at 3432.236 kb on - strand at 3432.260 kb on + strand at 3432.260 kb on + strand at 3432.260 kb on + strand at 3432.261 kb on - strand at 3432.261 kb on - strand at 3432.295 kb on - strand at 3432.315 kb on + strand at 3432.316 kb on - strand at 3432.316 kb on - strand at 3432.411 kb on + strand at 3432.411 kb on + strand at 3432.411 kb on + strand at 3432.412 kb on - strand at 3432.412 kb on - strand at 3432.412 kb on - strand at 3432.412 kb on - strand at 3432.543 kb on + strand, within Psyr_2862 at 3432.560 kb on - strand, within Psyr_2862 at 3432.560 kb on - strand, within Psyr_2862 at 3432.560 kb on - strand, within Psyr_2862 at 3432.560 kb on - strand, within Psyr_2862 at 3432.683 kb on + strand, within Psyr_2862 at 3432.781 kb on + strand, within Psyr_2862 at 3432.782 kb on - strand, within Psyr_2862 at 3432.789 kb on - strand, within Psyr_2862 at 3432.813 kb on + strand, within Psyr_2862 at 3432.813 kb on + strand, within Psyr_2862 at 3432.813 kb on + strand, within Psyr_2862 at 3432.858 kb on + strand, within Psyr_2862 at 3432.858 kb on + strand, within Psyr_2862 at 3432.858 kb on + strand, within Psyr_2862 at 3432.858 kb on + strand, within Psyr_2862 at 3432.858 kb on + strand, within Psyr_2862 at 3432.859 kb on - strand, within Psyr_2862 at 3432.859 kb on - strand, within Psyr_2862 at 3433.024 kb on + strand, within Psyr_2862 at 3433.039 kb on + strand, within Psyr_2862 at 3433.039 kb on + strand, within Psyr_2862 at 3433.066 kb on + strand, within Psyr_2862 at 3433.179 kb on + strand at 3433.179 kb on + strand at 3433.179 kb on + strand at 3433.180 kb on - strand at 3433.180 kb on - strand at 3433.180 kb on - strand at 3433.180 kb on - strand at 3433.196 kb on + strand at 3433.623 kb on + strand at 3433.663 kb on + strand at 3433.663 kb on + strand at 3433.663 kb on + strand at 3433.664 kb on - strand at 3433.664 kb on - strand at 3433.664 kb on - strand at 3433.665 kb on + strand at 3434.022 kb on + strand, within Psyr_2863 at 3434.022 kb on + strand, within Psyr_2863 at 3434.022 kb on + strand, within Psyr_2863 at 3434.022 kb on + strand, within Psyr_2863 at 3434.022 kb on + strand, within Psyr_2863 at 3434.023 kb on - strand, within Psyr_2863 at 3434.023 kb on - strand, within Psyr_2863 at 3434.023 kb on - strand, within Psyr_2863 at 3434.190 kb on + strand, within Psyr_2863
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,431,430 + Psyr_2860 0.30 +3.1 3,431,431 - Psyr_2860 0.30 +3.8 3,431,591 + Psyr_2860 0.72 -0.2 3,431,647 + Psyr_2860 0.87 +1.8 3,431,668 + -2.9 3,431,668 + -0.3 3,431,668 + +1.4 3,431,669 - +1.8 3,431,669 - -1.1 3,431,688 + +1.8 3,431,689 - -2.5 3,431,750 - +3.5 3,431,750 - +1.4 3,432,137 - +1.8 3,432,147 + +2.1 3,432,147 + +0.8 3,432,148 - +1.8 3,432,148 - +1.4 3,432,148 - -0.5 3,432,153 + +2.8 3,432,153 + -1.3 3,432,156 + -1.9 3,432,236 - +1.8 3,432,260 + -0.2 3,432,260 + -1.6 3,432,260 + +1.4 3,432,261 - -2.2 3,432,261 - -2.3 3,432,295 - -0.5 3,432,315 + -1.5 3,432,316 - +1.4 3,432,316 - +4.2 3,432,411 + +2.8 3,432,411 + -0.4 3,432,411 + +1.8 3,432,412 - -3.0 3,432,412 - -2.8 3,432,412 - +3.0 3,432,412 - -1.3 3,432,543 + Psyr_2862 0.18 +0.1 3,432,560 - Psyr_2862 0.20 -0.1 3,432,560 - Psyr_2862 0.20 -0.1 3,432,560 - Psyr_2862 0.20 +2.4 3,432,560 - Psyr_2862 0.20 +0.6 3,432,683 + Psyr_2862 0.35 +3.0 3,432,781 + Psyr_2862 0.47 -0.6 3,432,782 - Psyr_2862 0.47 +2.1 3,432,789 - Psyr_2862 0.48 -1.5 3,432,813 + Psyr_2862 0.51 +0.7 3,432,813 + Psyr_2862 0.51 +4.0 3,432,813 + Psyr_2862 0.51 +0.4 3,432,858 + Psyr_2862 0.57 +1.8 3,432,858 + Psyr_2862 0.57 -0.4 3,432,858 + Psyr_2862 0.57 -2.8 3,432,858 + Psyr_2862 0.57 +3.1 3,432,858 + Psyr_2862 0.57 +1.8 3,432,859 - Psyr_2862 0.57 -0.9 3,432,859 - Psyr_2862 0.57 +2.8 3,433,024 + Psyr_2862 0.77 +1.8 3,433,039 + Psyr_2862 0.79 -0.8 3,433,039 + Psyr_2862 0.79 -0.7 3,433,066 + Psyr_2862 0.83 +3.6 3,433,179 + +2.8 3,433,179 + -4.3 3,433,179 + +0.8 3,433,180 - -1.2 3,433,180 - +3.2 3,433,180 - +0.6 3,433,180 - -3.8 3,433,196 + -1.7 3,433,623 + -4.4 3,433,663 + -1.7 3,433,663 + -2.6 3,433,663 + -0.2 3,433,664 - +3.1 3,433,664 - +2.1 3,433,664 - +2.6 3,433,665 + +2.8 3,434,022 + Psyr_2863 0.22 +3.2 3,434,022 + Psyr_2863 0.22 +3.2 3,434,022 + Psyr_2863 0.22 -0.2 3,434,022 + Psyr_2863 0.22 -0.2 3,434,022 + Psyr_2863 0.22 -1.0 3,434,023 - Psyr_2863 0.22 -1.4 3,434,023 - Psyr_2863 0.22 -0.9 3,434,023 - Psyr_2863 0.22 +3.1 3,434,190 + Psyr_2863 0.32 -1.7
Or see this region's nucleotide sequence