Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2854 and Psyr_2855 are separated by 23 nucleotides Psyr_2855 and Psyr_2856 are separated by 158 nucleotides
Psyr_2854: Psyr_2854 - conserved hypothetical protein, at 3,425,751 to 3,426,734
_2854
Psyr_2855: Psyr_2855 - methionine synthase (B12-independent), at 3,426,758 to 3,427,789
_2855
Psyr_2856: Psyr_2856 - Chitin-binding, domain 3, at 3,427,948 to 3,428,691
_2856
Position (kb)
3426
3427
3428 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 3425.802 kb on + strand at 3425.883 kb on - strand, within Psyr_2854 at 3425.885 kb on + strand, within Psyr_2854 at 3425.954 kb on + strand, within Psyr_2854 at 3425.996 kb on + strand, within Psyr_2854 at 3426.109 kb on - strand, within Psyr_2854 at 3426.238 kb on - strand, within Psyr_2854 at 3426.297 kb on + strand, within Psyr_2854 at 3426.297 kb on + strand, within Psyr_2854 at 3426.298 kb on - strand, within Psyr_2854 at 3426.328 kb on + strand, within Psyr_2854 at 3426.373 kb on - strand, within Psyr_2854 at 3426.403 kb on - strand, within Psyr_2854 at 3426.468 kb on + strand, within Psyr_2854 at 3426.469 kb on - strand, within Psyr_2854 at 3426.486 kb on - strand, within Psyr_2854 at 3426.540 kb on + strand, within Psyr_2854 at 3426.540 kb on + strand, within Psyr_2854 at 3426.540 kb on + strand, within Psyr_2854 at 3426.541 kb on - strand, within Psyr_2854 at 3426.541 kb on - strand, within Psyr_2854 at 3426.541 kb on - strand, within Psyr_2854 at 3426.541 kb on - strand, within Psyr_2854 at 3426.618 kb on + strand, within Psyr_2854 at 3426.619 kb on - strand, within Psyr_2854 at 3426.690 kb on + strand at 3426.690 kb on + strand at 3426.740 kb on + strand at 3426.751 kb on + strand at 3426.751 kb on + strand at 3426.757 kb on + strand at 3426.970 kb on + strand, within Psyr_2855 at 3426.970 kb on + strand, within Psyr_2855 at 3426.976 kb on + strand, within Psyr_2855 at 3426.976 kb on + strand, within Psyr_2855 at 3427.021 kb on + strand, within Psyr_2855 at 3427.194 kb on - strand, within Psyr_2855 at 3427.369 kb on + strand at 3427.390 kb on + strand, within Psyr_2855 at 3427.390 kb on + strand, within Psyr_2855 at 3427.390 kb on + strand, within Psyr_2855 at 3427.391 kb on - strand, within Psyr_2855 at 3427.391 kb on - strand, within Psyr_2855 at 3427.461 kb on - strand, within Psyr_2855 at 3427.799 kb on + strand at 3427.799 kb on + strand at 3427.799 kb on + strand at 3427.799 kb on + strand at 3427.800 kb on - strand at 3427.800 kb on - strand at 3427.800 kb on - strand at 3427.876 kb on + strand at 3427.876 kb on + strand at 3427.876 kb on + strand at 3427.876 kb on + strand at 3427.876 kb on + strand at 3427.876 kb on + strand at 3427.876 kb on + strand at 3427.877 kb on - strand at 3427.941 kb on + strand at 3428.079 kb on + strand, within Psyr_2856 at 3428.080 kb on - strand, within Psyr_2856 at 3428.080 kb on - strand, within Psyr_2856 at 3428.192 kb on + strand, within Psyr_2856 at 3428.192 kb on + strand, within Psyr_2856 at 3428.193 kb on - strand, within Psyr_2856 at 3428.193 kb on - strand, within Psyr_2856 at 3428.193 kb on - strand, within Psyr_2856 at 3428.193 kb on - strand, within Psyr_2856 at 3428.193 kb on - strand at 3428.193 kb on - strand, within Psyr_2856 at 3428.256 kb on + strand, within Psyr_2856 at 3428.257 kb on - strand, within Psyr_2856 at 3428.284 kb on + strand, within Psyr_2856 at 3428.284 kb on + strand, within Psyr_2856 at 3428.285 kb on - strand, within Psyr_2856 at 3428.285 kb on - strand, within Psyr_2856 at 3428.285 kb on - strand, within Psyr_2856 at 3428.294 kb on + strand, within Psyr_2856 at 3428.304 kb on + strand, within Psyr_2856 at 3428.304 kb on + strand, within Psyr_2856 at 3428.305 kb on - strand, within Psyr_2856 at 3428.377 kb on + strand, within Psyr_2856 at 3428.446 kb on + strand, within Psyr_2856 at 3428.446 kb on + strand, within Psyr_2856 at 3428.446 kb on + strand, within Psyr_2856 at 3428.446 kb on + strand, within Psyr_2856 at 3428.446 kb on + strand, within Psyr_2856 at 3428.447 kb on - strand at 3428.447 kb on - strand, within Psyr_2856 at 3428.447 kb on - strand, within Psyr_2856 at 3428.505 kb on + strand, within Psyr_2856 at 3428.508 kb on + strand, within Psyr_2856 at 3428.509 kb on - strand, within Psyr_2856 at 3428.509 kb on - strand, within Psyr_2856 at 3428.624 kb on + strand at 3428.625 kb on - strand at 3428.625 kb on - strand at 3428.625 kb on - strand at 3428.650 kb on + strand at 3428.697 kb on + strand at 3428.698 kb on - strand at 3428.707 kb on + strand at 3428.708 kb on - strand at 3428.767 kb on - strand at 3428.770 kb on + strand at 3428.771 kb on - strand at 3428.771 kb on - strand at 3428.771 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,425,802 + +3.8 3,425,883 - Psyr_2854 0.13 +0.8 3,425,885 + Psyr_2854 0.14 -0.2 3,425,954 + Psyr_2854 0.21 -2.4 3,425,996 + Psyr_2854 0.25 -1.4 3,426,109 - Psyr_2854 0.36 +2.8 3,426,238 - Psyr_2854 0.49 +0.8 3,426,297 + Psyr_2854 0.55 -1.3 3,426,297 + Psyr_2854 0.55 -0.9 3,426,298 - Psyr_2854 0.56 +0.8 3,426,328 + Psyr_2854 0.59 -2.3 3,426,373 - Psyr_2854 0.63 -2.2 3,426,403 - Psyr_2854 0.66 +1.8 3,426,468 + Psyr_2854 0.73 +2.1 3,426,469 - Psyr_2854 0.73 -5.4 3,426,486 - Psyr_2854 0.75 +2.4 3,426,540 + Psyr_2854 0.80 -4.1 3,426,540 + Psyr_2854 0.80 -1.1 3,426,540 + Psyr_2854 0.80 -1.8 3,426,541 - Psyr_2854 0.80 -3.0 3,426,541 - Psyr_2854 0.80 +0.8 3,426,541 - Psyr_2854 0.80 -0.2 3,426,541 - Psyr_2854 0.80 +0.8 3,426,618 + Psyr_2854 0.88 +2.6 3,426,619 - Psyr_2854 0.88 +0.8 3,426,690 + -2.1 3,426,690 + +2.6 3,426,740 + +2.8 3,426,751 + +3.1 3,426,751 + -0.2 3,426,757 + -4.0 3,426,970 + Psyr_2855 0.21 -1.5 3,426,970 + Psyr_2855 0.21 -0.2 3,426,976 + Psyr_2855 0.21 +1.2 3,426,976 + Psyr_2855 0.21 -1.8 3,427,021 + Psyr_2855 0.25 -0.2 3,427,194 - Psyr_2855 0.42 -1.6 3,427,369 + -0.2 3,427,390 + Psyr_2855 0.61 +1.4 3,427,390 + Psyr_2855 0.61 -3.1 3,427,390 + Psyr_2855 0.61 -3.2 3,427,391 - Psyr_2855 0.61 -2.3 3,427,391 - Psyr_2855 0.61 -2.4 3,427,461 - Psyr_2855 0.68 -0.2 3,427,799 + -2.1 3,427,799 + -1.2 3,427,799 + -2.2 3,427,799 + +0.8 3,427,800 - -2.0 3,427,800 - +2.1 3,427,800 - +0.8 3,427,876 + -1.9 3,427,876 + -1.7 3,427,876 + -0.1 3,427,876 + +1.8 3,427,876 + -1.1 3,427,876 + +3.5 3,427,876 + -0.2 3,427,877 - +1.4 3,427,941 + -0.2 3,428,079 + Psyr_2856 0.18 -0.2 3,428,080 - Psyr_2856 0.18 -0.2 3,428,080 - Psyr_2856 0.18 +3.5 3,428,192 + Psyr_2856 0.33 +0.8 3,428,192 + Psyr_2856 0.33 -0.2 3,428,193 - Psyr_2856 0.33 +3.4 3,428,193 - Psyr_2856 0.33 +2.8 3,428,193 - Psyr_2856 0.33 +0.6 3,428,193 - Psyr_2856 0.33 +2.4 3,428,193 - +0.8 3,428,193 - Psyr_2856 0.33 +2.4 3,428,256 + Psyr_2856 0.41 -3.6 3,428,257 - Psyr_2856 0.42 +1.8 3,428,284 + Psyr_2856 0.45 +2.6 3,428,284 + Psyr_2856 0.45 -0.7 3,428,285 - Psyr_2856 0.45 -1.7 3,428,285 - Psyr_2856 0.45 -0.6 3,428,285 - Psyr_2856 0.45 -1.0 3,428,294 + Psyr_2856 0.47 -1.7 3,428,304 + Psyr_2856 0.48 +1.4 3,428,304 + Psyr_2856 0.48 +0.4 3,428,305 - Psyr_2856 0.48 -0.2 3,428,377 + Psyr_2856 0.58 +4.3 3,428,446 + Psyr_2856 0.67 -1.3 3,428,446 + Psyr_2856 0.67 -0.8 3,428,446 + Psyr_2856 0.67 -0.3 3,428,446 + Psyr_2856 0.67 -0.9 3,428,446 + Psyr_2856 0.67 +1.2 3,428,447 - +1.4 3,428,447 - Psyr_2856 0.67 +2.1 3,428,447 - Psyr_2856 0.67 +1.8 3,428,505 + Psyr_2856 0.75 -4.9 3,428,508 + Psyr_2856 0.75 -2.6 3,428,509 - Psyr_2856 0.75 -0.6 3,428,509 - Psyr_2856 0.75 -1.1 3,428,624 + -1.2 3,428,625 - -0.1 3,428,625 - -0.7 3,428,625 - +0.1 3,428,650 + -0.2 3,428,697 + +2.6 3,428,698 - +3.1 3,428,707 + -1.6 3,428,708 - -2.5 3,428,767 - -1.0 3,428,770 + -0.7 3,428,771 - -0.2 3,428,771 - +0.1 3,428,771 - -0.8
Or see this region's nucleotide sequence