Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2821 and Psyr_2822 overlap by 4 nucleotides Psyr_2822 and Psyr_2823 are separated by 81 nucleotides Psyr_2823 and Psyr_2824 are separated by 527 nucleotides
Psyr_2821: Psyr_2821 - conserved hypothetical protein, at 3,395,112 to 3,396,773
_2821
Psyr_2822: Psyr_2822 - hypothetical protein, at 3,396,770 to 3,397,132
_2822
Psyr_2823: Psyr_2823 - hypothetical protein, at 3,397,214 to 3,397,597
_2823
Psyr_2824: Psyr_2824 - conserved hypothetical protein, at 3,398,125 to 3,398,907
_2824
Position (kb)
3397
3398 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3396.599 kb on + strand, within Psyr_2821 at 3396.600 kb on - strand, within Psyr_2821 at 3396.600 kb on - strand, within Psyr_2821 at 3396.600 kb on - strand, within Psyr_2821 at 3396.600 kb on - strand, within Psyr_2821 at 3396.600 kb on - strand, within Psyr_2821 at 3396.868 kb on - strand, within Psyr_2822 at 3396.938 kb on + strand, within Psyr_2822 at 3396.938 kb on + strand, within Psyr_2822 at 3396.962 kb on + strand, within Psyr_2822 at 3396.962 kb on + strand, within Psyr_2822 at 3396.962 kb on + strand, within Psyr_2822 at 3396.963 kb on - strand, within Psyr_2822 at 3396.963 kb on - strand, within Psyr_2822 at 3397.094 kb on + strand, within Psyr_2822 at 3397.094 kb on + strand, within Psyr_2822 at 3397.106 kb on + strand at 3397.211 kb on + strand at 3397.558 kb on + strand, within Psyr_2823 at 3397.559 kb on - strand at 3397.616 kb on + strand at 3397.617 kb on - strand at 3397.617 kb on - strand at 3397.617 kb on - strand at 3397.617 kb on - strand at 3397.617 kb on - strand at 3397.621 kb on - strand at 3397.664 kb on + strand at 3397.664 kb on + strand at 3397.664 kb on + strand at 3397.664 kb on + strand at 3397.665 kb on - strand at 3397.721 kb on + strand at 3397.757 kb on - strand at 3397.821 kb on + strand at 3397.822 kb on - strand at 3397.852 kb on + strand at 3397.853 kb on - strand at 3397.975 kb on - strand at 3398.122 kb on - strand at 3398.154 kb on + strand at 3398.181 kb on + strand at 3398.244 kb on + strand, within Psyr_2824 at 3398.262 kb on + strand, within Psyr_2824 at 3398.263 kb on - strand, within Psyr_2824 at 3398.263 kb on - strand, within Psyr_2824 at 3398.263 kb on - strand, within Psyr_2824 at 3398.424 kb on + strand, within Psyr_2824 at 3398.428 kb on - strand, within Psyr_2824 at 3398.433 kb on + strand, within Psyr_2824 at 3398.451 kb on - strand, within Psyr_2824 at 3398.488 kb on - strand, within Psyr_2824 at 3398.553 kb on + strand, within Psyr_2824 at 3398.554 kb on - strand, within Psyr_2824 at 3398.558 kb on + strand, within Psyr_2824
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,396,599 + Psyr_2821 0.89 -0.9 3,396,600 - Psyr_2821 0.90 -0.8 3,396,600 - Psyr_2821 0.90 +3.4 3,396,600 - Psyr_2821 0.90 -0.4 3,396,600 - Psyr_2821 0.90 -1.1 3,396,600 - Psyr_2821 0.90 -2.5 3,396,868 - Psyr_2822 0.27 +3.9 3,396,938 + Psyr_2822 0.46 +2.6 3,396,938 + Psyr_2822 0.46 -0.2 3,396,962 + Psyr_2822 0.53 -1.5 3,396,962 + Psyr_2822 0.53 +4.0 3,396,962 + Psyr_2822 0.53 +1.1 3,396,963 - Psyr_2822 0.53 -1.1 3,396,963 - Psyr_2822 0.53 +3.2 3,397,094 + Psyr_2822 0.89 -0.4 3,397,094 + Psyr_2822 0.89 -1.3 3,397,106 + -2.4 3,397,211 + -2.5 3,397,558 + Psyr_2823 0.90 -2.1 3,397,559 - -0.2 3,397,616 + -1.8 3,397,617 - +2.1 3,397,617 - +3.6 3,397,617 - -0.5 3,397,617 - -0.3 3,397,617 - -2.4 3,397,621 - +2.8 3,397,664 + -0.2 3,397,664 + +1.8 3,397,664 + +0.8 3,397,664 + -0.2 3,397,665 - -0.5 3,397,721 + -2.5 3,397,757 - +3.6 3,397,821 + -0.2 3,397,822 - -0.4 3,397,852 + -0.2 3,397,853 - +3.2 3,397,975 - -0.2 3,398,122 - +1.8 3,398,154 + -3.4 3,398,181 + -0.2 3,398,244 + Psyr_2824 0.15 -4.2 3,398,262 + Psyr_2824 0.17 -2.0 3,398,263 - Psyr_2824 0.18 +0.8 3,398,263 - Psyr_2824 0.18 -2.1 3,398,263 - Psyr_2824 0.18 +2.6 3,398,424 + Psyr_2824 0.38 -0.2 3,398,428 - Psyr_2824 0.39 +1.8 3,398,433 + Psyr_2824 0.39 -0.2 3,398,451 - Psyr_2824 0.42 -0.8 3,398,488 - Psyr_2824 0.46 +1.8 3,398,553 + Psyr_2824 0.55 -3.7 3,398,554 - Psyr_2824 0.55 -2.1 3,398,558 + Psyr_2824 0.55 -3.7
Or see this region's nucleotide sequence