Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2780 and Psyr_2781 are separated by 10 nucleotides Psyr_2781 and Psyr_2782 are separated by 62 nucleotides Psyr_2782 and Psyr_2783 are separated by 3 nucleotides Psyr_2783 and Psyr_2784 are separated by 71 nucleotides Psyr_2784 and Psyr_2785 overlap by 4 nucleotides
Psyr_2780: Psyr_2780 - Phage tail tape measure protein lambda, at 3,364,992 to 3,368,477
_2780
Psyr_2781: Psyr_2781 - hypothetical protein, at 3,368,488 to 3,368,736
_2781
Psyr_2782: Psyr_2782 - hypothetical protein, at 3,368,799 to 3,369,182
_2782
Psyr_2783: Psyr_2783 - conserved hypothetical protein, at 3,369,186 to 3,369,842
_2783
Psyr_2784: Psyr_2784 - phage-related conserved hypothetical protein, at 3,369,914 to 3,370,324
_2784
Psyr_2785: Psyr_2785 - conserved hypothetical protein, at 3,370,321 to 3,370,977
_2785
Position (kb)
3369
3370 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3368.210 kb on + strand at 3368.210 kb on + strand at 3368.211 kb on - strand at 3368.557 kb on + strand, within Psyr_2781 at 3368.557 kb on + strand, within Psyr_2781 at 3368.558 kb on - strand, within Psyr_2781 at 3368.609 kb on + strand, within Psyr_2781 at 3368.610 kb on - strand, within Psyr_2781 at 3368.777 kb on + strand at 3368.777 kb on + strand at 3368.837 kb on - strand at 3368.980 kb on - strand, within Psyr_2782 at 3369.039 kb on - strand, within Psyr_2782 at 3369.039 kb on - strand, within Psyr_2782 at 3369.080 kb on + strand, within Psyr_2782 at 3369.080 kb on + strand, within Psyr_2782 at 3369.082 kb on + strand, within Psyr_2782 at 3369.082 kb on + strand, within Psyr_2782 at 3369.082 kb on + strand, within Psyr_2782 at 3369.082 kb on + strand, within Psyr_2782 at 3369.082 kb on + strand, within Psyr_2782 at 3369.082 kb on + strand, within Psyr_2782 at 3369.082 kb on + strand, within Psyr_2782 at 3369.082 kb on + strand, within Psyr_2782 at 3369.082 kb on + strand, within Psyr_2782 at 3369.082 kb on + strand, within Psyr_2782 at 3369.082 kb on + strand, within Psyr_2782 at 3369.082 kb on + strand, within Psyr_2782 at 3369.083 kb on - strand, within Psyr_2782 at 3369.083 kb on - strand, within Psyr_2782 at 3369.083 kb on - strand, within Psyr_2782 at 3369.083 kb on - strand, within Psyr_2782 at 3369.083 kb on - strand, within Psyr_2782 at 3369.083 kb on - strand, within Psyr_2782 at 3369.083 kb on - strand, within Psyr_2782 at 3369.295 kb on + strand, within Psyr_2783 at 3369.296 kb on - strand, within Psyr_2783 at 3369.304 kb on + strand, within Psyr_2783 at 3369.304 kb on + strand, within Psyr_2783 at 3369.305 kb on - strand, within Psyr_2783 at 3369.388 kb on + strand, within Psyr_2783 at 3369.388 kb on + strand, within Psyr_2783 at 3369.388 kb on + strand, within Psyr_2783 at 3369.389 kb on - strand, within Psyr_2783 at 3369.389 kb on - strand, within Psyr_2783 at 3369.389 kb on - strand, within Psyr_2783 at 3369.389 kb on - strand, within Psyr_2783 at 3369.915 kb on + strand at 3369.915 kb on + strand at 3369.915 kb on + strand at 3369.916 kb on - strand at 3369.916 kb on - strand at 3369.916 kb on - strand at 3369.916 kb on - strand at 3369.961 kb on + strand, within Psyr_2784 at 3369.961 kb on + strand, within Psyr_2784 at 3370.041 kb on + strand, within Psyr_2784 at 3370.042 kb on - strand, within Psyr_2784 at 3370.140 kb on + strand, within Psyr_2784 at 3370.140 kb on + strand, within Psyr_2784 at 3370.140 kb on + strand, within Psyr_2784 at 3370.141 kb on - strand, within Psyr_2784 at 3370.141 kb on - strand, within Psyr_2784 at 3370.206 kb on + strand, within Psyr_2784 at 3370.206 kb on + strand, within Psyr_2784 at 3370.206 kb on + strand, within Psyr_2784 at 3370.324 kb on + strand at 3370.325 kb on - strand at 3370.457 kb on + strand, within Psyr_2785 at 3370.458 kb on - strand, within Psyr_2785 at 3370.458 kb on - strand, within Psyr_2785 at 3370.604 kb on + strand, within Psyr_2785 at 3370.604 kb on + strand, within Psyr_2785 at 3370.604 kb on + strand, within Psyr_2785 at 3370.604 kb on + strand, within Psyr_2785 at 3370.605 kb on - strand, within Psyr_2785 at 3370.618 kb on + strand, within Psyr_2785 at 3370.618 kb on + strand, within Psyr_2785 at 3370.619 kb on - strand, within Psyr_2785 at 3370.676 kb on + strand, within Psyr_2785 at 3370.677 kb on - strand, within Psyr_2785 at 3370.677 kb on - strand, within Psyr_2785
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,368,210 + +2.4 3,368,210 + +1.9 3,368,211 - +2.6 3,368,557 + Psyr_2781 0.28 +0.5 3,368,557 + Psyr_2781 0.28 +2.8 3,368,558 - Psyr_2781 0.28 +0.9 3,368,609 + Psyr_2781 0.49 -0.4 3,368,610 - Psyr_2781 0.49 +0.0 3,368,777 + -1.4 3,368,777 + -0.8 3,368,837 - +3.3 3,368,980 - Psyr_2782 0.47 -0.9 3,369,039 - Psyr_2782 0.62 +1.4 3,369,039 - Psyr_2782 0.62 -1.5 3,369,080 + Psyr_2782 0.73 -1.5 3,369,080 + Psyr_2782 0.73 -1.3 3,369,082 + Psyr_2782 0.74 -1.9 3,369,082 + Psyr_2782 0.74 +2.6 3,369,082 + Psyr_2782 0.74 -1.7 3,369,082 + Psyr_2782 0.74 -0.9 3,369,082 + Psyr_2782 0.74 +3.0 3,369,082 + Psyr_2782 0.74 +0.4 3,369,082 + Psyr_2782 0.74 +0.9 3,369,082 + Psyr_2782 0.74 +2.4 3,369,082 + Psyr_2782 0.74 +2.4 3,369,082 + Psyr_2782 0.74 +2.6 3,369,082 + Psyr_2782 0.74 +2.4 3,369,082 + Psyr_2782 0.74 +2.6 3,369,083 - Psyr_2782 0.74 +3.0 3,369,083 - Psyr_2782 0.74 -0.6 3,369,083 - Psyr_2782 0.74 +3.8 3,369,083 - Psyr_2782 0.74 -0.5 3,369,083 - Psyr_2782 0.74 +0.5 3,369,083 - Psyr_2782 0.74 +2.4 3,369,083 - Psyr_2782 0.74 +2.1 3,369,295 + Psyr_2783 0.17 +0.0 3,369,296 - Psyr_2783 0.17 -2.5 3,369,304 + Psyr_2783 0.18 +0.1 3,369,304 + Psyr_2783 0.18 -0.4 3,369,305 - Psyr_2783 0.18 +0.1 3,369,388 + Psyr_2783 0.31 +3.0 3,369,388 + Psyr_2783 0.31 +2.8 3,369,388 + Psyr_2783 0.31 +2.1 3,369,389 - Psyr_2783 0.31 +0.6 3,369,389 - Psyr_2783 0.31 +3.5 3,369,389 - Psyr_2783 0.31 +2.1 3,369,389 - Psyr_2783 0.31 -1.1 3,369,915 + +3.3 3,369,915 + -0.6 3,369,915 + +1.8 3,369,916 - -1.5 3,369,916 - -2.1 3,369,916 - -0.2 3,369,916 - -2.1 3,369,961 + Psyr_2784 0.11 -0.4 3,369,961 + Psyr_2784 0.11 -0.8 3,370,041 + Psyr_2784 0.31 +0.6 3,370,042 - Psyr_2784 0.31 -1.9 3,370,140 + Psyr_2784 0.55 -1.2 3,370,140 + Psyr_2784 0.55 -0.2 3,370,140 + Psyr_2784 0.55 -3.0 3,370,141 - Psyr_2784 0.55 -0.9 3,370,141 - Psyr_2784 0.55 -3.4 3,370,206 + Psyr_2784 0.71 -0.6 3,370,206 + Psyr_2784 0.71 -2.3 3,370,206 + Psyr_2784 0.71 -0.6 3,370,324 + +3.0 3,370,325 - -0.8 3,370,457 + Psyr_2785 0.21 -0.5 3,370,458 - Psyr_2785 0.21 -1.4 3,370,458 - Psyr_2785 0.21 +0.5 3,370,604 + Psyr_2785 0.43 -0.2 3,370,604 + Psyr_2785 0.43 +3.5 3,370,604 + Psyr_2785 0.43 -1.9 3,370,604 + Psyr_2785 0.43 +4.1 3,370,605 - Psyr_2785 0.43 -0.4 3,370,618 + Psyr_2785 0.45 +0.2 3,370,618 + Psyr_2785 0.45 +0.8 3,370,619 - Psyr_2785 0.45 +2.1 3,370,676 + Psyr_2785 0.54 +1.4 3,370,677 - Psyr_2785 0.54 +1.4 3,370,677 - Psyr_2785 0.54 +1.8
Or see this region's nucleotide sequence