Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2767 and Psyr_2768 are separated by 295 nucleotides Psyr_2768 and Psyr_2769 are separated by 13 nucleotides Psyr_2769 and Psyr_2770 overlap by 1 nucleotides Psyr_2770 and Psyr_2771 are separated by 25 nucleotides
Psyr_2767: Psyr_2767 - Glycoside hydrolase, family 19, at 3,353,250 to 3,353,783
_2767
Psyr_2768: Psyr_2768 - tail fiber assembly domain protein, at 3,354,079 to 3,354,447
_2768
Psyr_2769: Psyr_2769 - conserved hypothetical protein, at 3,354,461 to 3,354,775
_2769
Psyr_2770: Psyr_2770 - tail fiber domain protein, at 3,354,775 to 3,355,878
_2770
Psyr_2771: Psyr_2771 - conserved domain protein, at 3,355,904 to 3,356,590
_2771
Position (kb)
3354
3355
3356 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 3353.777 kb on + strand at 3353.778 kb on - strand at 3353.807 kb on + strand at 3353.808 kb on - strand at 3353.844 kb on + strand at 3353.844 kb on + strand at 3353.845 kb on - strand at 3353.845 kb on - strand at 3353.863 kb on + strand at 3353.863 kb on + strand at 3353.864 kb on - strand at 3353.874 kb on + strand at 3353.874 kb on + strand at 3353.875 kb on - strand at 3353.889 kb on + strand at 3353.889 kb on + strand at 3353.943 kb on + strand at 3353.943 kb on + strand at 3353.943 kb on + strand at 3353.948 kb on - strand at 3353.990 kb on + strand at 3353.990 kb on + strand at 3353.990 kb on + strand at 3353.991 kb on - strand at 3353.991 kb on - strand at 3353.991 kb on - strand at 3354.046 kb on + strand at 3354.315 kb on + strand, within Psyr_2768 at 3354.421 kb on + strand at 3354.422 kb on - strand at 3354.422 kb on - strand at 3354.447 kb on + strand at 3354.447 kb on + strand at 3354.447 kb on + strand at 3354.447 kb on + strand at 3354.447 kb on + strand at 3354.447 kb on + strand at 3354.448 kb on - strand at 3354.555 kb on + strand, within Psyr_2769 at 3354.555 kb on + strand, within Psyr_2769 at 3354.556 kb on - strand, within Psyr_2769 at 3354.556 kb on - strand, within Psyr_2769 at 3354.585 kb on - strand, within Psyr_2769 at 3354.610 kb on + strand, within Psyr_2769 at 3354.659 kb on - strand, within Psyr_2769 at 3354.691 kb on + strand, within Psyr_2769 at 3354.691 kb on + strand, within Psyr_2769 at 3354.728 kb on + strand, within Psyr_2769 at 3354.777 kb on - strand at 3354.798 kb on + strand at 3354.798 kb on + strand at 3354.798 kb on + strand at 3354.798 kb on + strand at 3354.799 kb on - strand at 3354.800 kb on + strand at 3354.800 kb on + strand at 3354.801 kb on - strand at 3354.801 kb on - strand at 3354.801 kb on - strand at 3354.870 kb on + strand at 3354.871 kb on - strand at 3354.896 kb on + strand, within Psyr_2770 at 3354.904 kb on - strand, within Psyr_2770 at 3354.904 kb on - strand, within Psyr_2770 at 3354.981 kb on - strand, within Psyr_2770 at 3355.096 kb on + strand, within Psyr_2770 at 3355.097 kb on - strand, within Psyr_2770 at 3355.160 kb on + strand, within Psyr_2770 at 3355.160 kb on + strand, within Psyr_2770 at 3355.161 kb on - strand, within Psyr_2770 at 3355.175 kb on - strand, within Psyr_2770 at 3355.297 kb on - strand, within Psyr_2770 at 3355.300 kb on + strand, within Psyr_2770 at 3355.301 kb on - strand, within Psyr_2770 at 3355.301 kb on - strand, within Psyr_2770 at 3355.324 kb on - strand, within Psyr_2770 at 3355.340 kb on - strand, within Psyr_2770 at 3355.395 kb on + strand, within Psyr_2770 at 3355.593 kb on + strand, within Psyr_2770 at 3355.700 kb on + strand, within Psyr_2770 at 3355.701 kb on - strand, within Psyr_2770 at 3355.751 kb on + strand, within Psyr_2770 at 3355.751 kb on + strand, within Psyr_2770 at 3355.869 kb on - strand at 3356.010 kb on + strand, within Psyr_2771 at 3356.010 kb on + strand, within Psyr_2771 at 3356.011 kb on - strand, within Psyr_2771 at 3356.069 kb on + strand, within Psyr_2771 at 3356.070 kb on - strand, within Psyr_2771 at 3356.070 kb on - strand, within Psyr_2771 at 3356.074 kb on - strand, within Psyr_2771 at 3356.106 kb on + strand, within Psyr_2771 at 3356.109 kb on + strand, within Psyr_2771 at 3356.208 kb on - strand, within Psyr_2771 at 3356.239 kb on + strand, within Psyr_2771 at 3356.328 kb on + strand, within Psyr_2771 at 3356.328 kb on + strand, within Psyr_2771 at 3356.328 kb on + strand, within Psyr_2771 at 3356.328 kb on + strand, within Psyr_2771 at 3356.328 kb on + strand, within Psyr_2771 at 3356.328 kb on + strand, within Psyr_2771 at 3356.329 kb on - strand, within Psyr_2771 at 3356.329 kb on - strand, within Psyr_2771 at 3356.329 kb on - strand, within Psyr_2771 at 3356.329 kb on - strand, within Psyr_2771 at 3356.357 kb on + strand, within Psyr_2771 at 3356.393 kb on + strand, within Psyr_2771 at 3356.394 kb on - strand, within Psyr_2771 at 3356.454 kb on + strand, within Psyr_2771 at 3356.455 kb on - strand, within Psyr_2771 at 3356.455 kb on - strand, within Psyr_2771 at 3356.485 kb on + strand, within Psyr_2771 at 3356.486 kb on - strand, within Psyr_2771 at 3356.486 kb on - strand, within Psyr_2771 at 3356.544 kb on - strand at 3356.605 kb on - strand at 3356.619 kb on + strand at 3356.619 kb on + strand at 3356.619 kb on + strand at 3356.649 kb on - strand at 3356.667 kb on - strand at 3356.715 kb on + strand at 3356.716 kb on - strand at 3356.723 kb on + strand at 3356.724 kb on - strand at 3356.724 kb on - strand at 3356.724 kb on - strand at 3356.817 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,353,777 + +1.8 3,353,778 - +2.1 3,353,807 + +1.4 3,353,808 - -1.4 3,353,844 + -0.6 3,353,844 + -1.0 3,353,845 - +3.0 3,353,845 - -2.7 3,353,863 + +2.6 3,353,863 + -0.6 3,353,864 - -3.4 3,353,874 + +4.1 3,353,874 + +2.1 3,353,875 - -2.5 3,353,889 + -2.6 3,353,889 + -2.6 3,353,943 + -0.2 3,353,943 + +1.8 3,353,943 + -1.5 3,353,948 - -1.4 3,353,990 + +2.4 3,353,990 + +2.6 3,353,990 + +2.1 3,353,991 - -0.6 3,353,991 - -3.8 3,353,991 - -2.0 3,354,046 + -0.2 3,354,315 + Psyr_2768 0.64 +0.8 3,354,421 + +0.8 3,354,422 - -1.3 3,354,422 - +1.4 3,354,447 + -4.6 3,354,447 + -5.3 3,354,447 + -0.2 3,354,447 + -0.2 3,354,447 + +2.1 3,354,447 + -3.3 3,354,448 - +1.3 3,354,555 + Psyr_2769 0.30 +3.1 3,354,555 + Psyr_2769 0.30 +1.4 3,354,556 - Psyr_2769 0.30 -2.0 3,354,556 - Psyr_2769 0.30 -2.0 3,354,585 - Psyr_2769 0.39 +2.1 3,354,610 + Psyr_2769 0.47 -4.2 3,354,659 - Psyr_2769 0.63 -0.9 3,354,691 + Psyr_2769 0.73 -0.2 3,354,691 + Psyr_2769 0.73 +2.4 3,354,728 + Psyr_2769 0.85 -4.6 3,354,777 - -1.2 3,354,798 + -1.5 3,354,798 + -3.8 3,354,798 + -0.2 3,354,798 + +0.4 3,354,799 - -1.7 3,354,800 + +1.8 3,354,800 + -0.2 3,354,801 - +3.5 3,354,801 - -2.1 3,354,801 - -2.5 3,354,870 + -0.2 3,354,871 - -1.9 3,354,896 + Psyr_2770 0.11 -2.8 3,354,904 - Psyr_2770 0.12 +0.2 3,354,904 - Psyr_2770 0.12 -0.6 3,354,981 - Psyr_2770 0.19 -0.3 3,355,096 + Psyr_2770 0.29 +1.8 3,355,097 - Psyr_2770 0.29 -1.9 3,355,160 + Psyr_2770 0.35 +0.8 3,355,160 + Psyr_2770 0.35 +1.8 3,355,161 - Psyr_2770 0.35 -2.5 3,355,175 - Psyr_2770 0.36 -0.2 3,355,297 - Psyr_2770 0.47 -0.2 3,355,300 + Psyr_2770 0.48 -0.4 3,355,301 - Psyr_2770 0.48 -2.1 3,355,301 - Psyr_2770 0.48 +1.8 3,355,324 - Psyr_2770 0.50 -3.3 3,355,340 - Psyr_2770 0.51 -0.6 3,355,395 + Psyr_2770 0.56 -2.8 3,355,593 + Psyr_2770 0.74 -1.9 3,355,700 + Psyr_2770 0.84 -1.4 3,355,701 - Psyr_2770 0.84 +3.6 3,355,751 + Psyr_2770 0.88 +2.6 3,355,751 + Psyr_2770 0.88 -3.1 3,355,869 - +1.5 3,356,010 + Psyr_2771 0.15 -0.2 3,356,010 + Psyr_2771 0.15 +1.4 3,356,011 - Psyr_2771 0.16 +0.8 3,356,069 + Psyr_2771 0.24 +0.8 3,356,070 - Psyr_2771 0.24 +3.9 3,356,070 - Psyr_2771 0.24 -2.5 3,356,074 - Psyr_2771 0.25 -1.1 3,356,106 + Psyr_2771 0.29 +2.4 3,356,109 + Psyr_2771 0.30 -0.2 3,356,208 - Psyr_2771 0.44 -2.6 3,356,239 + Psyr_2771 0.49 -0.2 3,356,328 + Psyr_2771 0.62 -1.4 3,356,328 + Psyr_2771 0.62 +0.8 3,356,328 + Psyr_2771 0.62 -0.2 3,356,328 + Psyr_2771 0.62 +3.4 3,356,328 + Psyr_2771 0.62 -0.2 3,356,328 + Psyr_2771 0.62 -2.5 3,356,329 - Psyr_2771 0.62 -2.1 3,356,329 - Psyr_2771 0.62 +0.2 3,356,329 - Psyr_2771 0.62 -1.4 3,356,329 - Psyr_2771 0.62 -1.7 3,356,357 + Psyr_2771 0.66 +1.4 3,356,393 + Psyr_2771 0.71 +3.0 3,356,394 - Psyr_2771 0.71 -1.3 3,356,454 + Psyr_2771 0.80 +0.1 3,356,455 - Psyr_2771 0.80 -0.8 3,356,455 - Psyr_2771 0.80 -2.6 3,356,485 + Psyr_2771 0.85 -2.0 3,356,486 - Psyr_2771 0.85 -1.9 3,356,486 - Psyr_2771 0.85 -0.6 3,356,544 - -1.3 3,356,605 - +0.6 3,356,619 + -1.6 3,356,619 + -0.9 3,356,619 + +2.1 3,356,649 - +2.1 3,356,667 - -0.4 3,356,715 + +4.3 3,356,716 - -1.4 3,356,723 + +3.0 3,356,724 - +2.6 3,356,724 - -1.7 3,356,724 - -2.1 3,356,817 + +2.6
Or see this region's nucleotide sequence