Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2736 and Psyr_2737 are separated by 164 nucleotides Psyr_2737 and Psyr_2738 are separated by 50 nucleotides Psyr_2738 and Psyr_2739 are separated by 202 nucleotides Psyr_2739 and Psyr_2740 are separated by 142 nucleotides
Psyr_2736: Psyr_2736 - glycosidase, putative, at 3,321,952 to 3,324,015
_2736
Psyr_2737: Psyr_2737 - hypothetical protein, at 3,324,180 to 3,324,695
_2737
Psyr_2738: Psyr_2738 - regulatory protein, LysR, at 3,324,746 to 3,325,069
_2738
Psyr_2739: Psyr_2739 - ThiJ/PfpI, at 3,325,272 to 3,325,877
_2739
Psyr_2740: Psyr_2740 - transcriptional regulator, AraC family with amidase-like domain protein, at 3,326,020 to 3,326,973
_2740
Position (kb)
3324
3325
3326 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 3323.993 kb on + strand at 3323.993 kb on + strand at 3323.994 kb on - strand at 3323.994 kb on - strand at 3324.079 kb on + strand at 3324.079 kb on + strand at 3324.079 kb on + strand at 3324.079 kb on + strand at 3324.079 kb on + strand at 3324.080 kb on - strand at 3324.080 kb on - strand at 3324.080 kb on - strand at 3324.080 kb on - strand at 3324.080 kb on - strand at 3324.080 kb on - strand at 3324.080 kb on - strand at 3324.435 kb on + strand, within Psyr_2737 at 3324.496 kb on + strand, within Psyr_2737 at 3324.496 kb on + strand, within Psyr_2737 at 3324.564 kb on + strand, within Psyr_2737 at 3324.565 kb on - strand, within Psyr_2737 at 3324.565 kb on - strand, within Psyr_2737 at 3324.568 kb on + strand, within Psyr_2737 at 3324.569 kb on - strand, within Psyr_2737 at 3324.598 kb on + strand, within Psyr_2737 at 3324.652 kb on - strand at 3324.835 kb on + strand, within Psyr_2738 at 3324.835 kb on + strand, within Psyr_2738 at 3324.835 kb on + strand, within Psyr_2738 at 3324.835 kb on + strand, within Psyr_2738 at 3324.835 kb on + strand, within Psyr_2738 at 3324.836 kb on - strand, within Psyr_2738 at 3324.836 kb on - strand, within Psyr_2738 at 3324.836 kb on - strand, within Psyr_2738 at 3324.945 kb on + strand, within Psyr_2738 at 3324.946 kb on - strand, within Psyr_2738 at 3324.946 kb on - strand, within Psyr_2738 at 3324.946 kb on - strand, within Psyr_2738 at 3325.110 kb on + strand at 3325.110 kb on + strand at 3325.237 kb on + strand at 3325.237 kb on + strand at 3325.237 kb on + strand at 3325.238 kb on - strand at 3325.238 kb on - strand at 3325.238 kb on - strand at 3325.238 kb on - strand at 3325.313 kb on + strand at 3325.382 kb on + strand, within Psyr_2739 at 3325.383 kb on - strand, within Psyr_2739 at 3325.806 kb on + strand, within Psyr_2739 at 3325.806 kb on + strand, within Psyr_2739 at 3325.806 kb on + strand, within Psyr_2739 at 3325.806 kb on + strand, within Psyr_2739 at 3325.806 kb on + strand, within Psyr_2739 at 3325.807 kb on - strand, within Psyr_2739 at 3325.807 kb on - strand, within Psyr_2739 at 3325.855 kb on + strand at 3325.855 kb on + strand at 3325.855 kb on + strand at 3325.856 kb on - strand at 3325.856 kb on - strand at 3325.856 kb on - strand at 3325.856 kb on - strand at 3325.856 kb on - strand at 3325.859 kb on + strand at 3325.918 kb on + strand at 3325.919 kb on - strand at 3325.919 kb on - strand at 3325.919 kb on - strand at 3325.960 kb on + strand at 3325.984 kb on - strand at 3326.050 kb on + strand at 3326.050 kb on + strand at 3326.050 kb on + strand at 3326.050 kb on + strand at 3326.050 kb on + strand at 3326.051 kb on - strand at 3326.051 kb on - strand at 3326.051 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,323,993 + -1.0 3,323,993 + +2.1 3,323,994 - +2.6 3,323,994 - -1.9 3,324,079 + -0.1 3,324,079 + -0.4 3,324,079 + -1.6 3,324,079 + -1.8 3,324,079 + -2.5 3,324,080 - -1.9 3,324,080 - +3.6 3,324,080 - -1.0 3,324,080 - +1.4 3,324,080 - +3.6 3,324,080 - +3.4 3,324,080 - -1.3 3,324,435 + Psyr_2737 0.49 +0.8 3,324,496 + Psyr_2737 0.61 +1.8 3,324,496 + Psyr_2737 0.61 +0.8 3,324,564 + Psyr_2737 0.74 +0.8 3,324,565 - Psyr_2737 0.75 +2.4 3,324,565 - Psyr_2737 0.75 +2.8 3,324,568 + Psyr_2737 0.75 -1.4 3,324,569 - Psyr_2737 0.75 -0.4 3,324,598 + Psyr_2737 0.81 -1.8 3,324,652 - +0.2 3,324,835 + Psyr_2738 0.27 -0.2 3,324,835 + Psyr_2738 0.27 -1.3 3,324,835 + Psyr_2738 0.27 +1.4 3,324,835 + Psyr_2738 0.27 -2.1 3,324,835 + Psyr_2738 0.27 +2.1 3,324,836 - Psyr_2738 0.28 -3.6 3,324,836 - Psyr_2738 0.28 -5.0 3,324,836 - Psyr_2738 0.28 -3.4 3,324,945 + Psyr_2738 0.61 +3.1 3,324,946 - Psyr_2738 0.62 -0.4 3,324,946 - Psyr_2738 0.62 +3.0 3,324,946 - Psyr_2738 0.62 -0.2 3,325,110 + +2.1 3,325,110 + +1.8 3,325,237 + +2.6 3,325,237 + +2.6 3,325,237 + -0.5 3,325,238 - -0.9 3,325,238 - -1.5 3,325,238 - +3.7 3,325,238 - -1.9 3,325,313 + -1.2 3,325,382 + Psyr_2739 0.18 -3.6 3,325,383 - Psyr_2739 0.18 +1.4 3,325,806 + Psyr_2739 0.88 -3.0 3,325,806 + Psyr_2739 0.88 +1.8 3,325,806 + Psyr_2739 0.88 -0.5 3,325,806 + Psyr_2739 0.88 +3.0 3,325,806 + Psyr_2739 0.88 -1.4 3,325,807 - Psyr_2739 0.88 +2.4 3,325,807 - Psyr_2739 0.88 -0.3 3,325,855 + -0.7 3,325,855 + +2.4 3,325,855 + +0.8 3,325,856 - -0.2 3,325,856 - -0.2 3,325,856 - -2.0 3,325,856 - -0.2 3,325,856 - +0.8 3,325,859 + -1.1 3,325,918 + -0.6 3,325,919 - +0.5 3,325,919 - +0.0 3,325,919 - +4.4 3,325,960 + +0.8 3,325,984 - +1.4 3,326,050 + -2.1 3,326,050 + -0.9 3,326,050 + +0.2 3,326,050 + -0.8 3,326,050 + -0.2 3,326,051 - +2.6 3,326,051 - +1.4 3,326,051 - +2.8
Or see this region's nucleotide sequence