Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2718 and Psyr_2719 are separated by 321 nucleotides Psyr_2719 and Psyr_2720 are separated by 5 nucleotides Psyr_2720 and Psyr_2721 overlap by 4 nucleotides Psyr_2721 and Psyr_2722 are separated by 86 nucleotides
Psyr_2718: Psyr_2718 - Major royal jelly protein, at 3,300,251 to 3,301,435
_2718
Psyr_2719: Psyr_2719 - transcriptional regulator, TetR family, at 3,301,757 to 3,302,338
_2719
Psyr_2720: Psyr_2720 - Urea amidolyase-related protein, at 3,302,344 to 3,303,318
_2720
Psyr_2721: Psyr_2721 - Protein of unknown function DUF213, at 3,303,315 to 3,304,187
_2721
Psyr_2722: Psyr_2722 - LamB/YcsF, at 3,304,274 to 3,305,029
_2722
Position (kb)
3302
3303
3304 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 3301.625 kb on - strand at 3301.699 kb on + strand at 3301.700 kb on - strand at 3301.700 kb on - strand at 3301.765 kb on + strand at 3301.766 kb on - strand at 3301.766 kb on - strand at 3301.838 kb on + strand, within Psyr_2719 at 3301.839 kb on - strand, within Psyr_2719 at 3301.899 kb on - strand, within Psyr_2719 at 3301.899 kb on - strand, within Psyr_2719 at 3302.006 kb on + strand, within Psyr_2719 at 3302.009 kb on + strand, within Psyr_2719 at 3302.009 kb on + strand, within Psyr_2719 at 3302.009 kb on + strand, within Psyr_2719 at 3302.009 kb on + strand, within Psyr_2719 at 3302.009 kb on + strand, within Psyr_2719 at 3302.010 kb on - strand, within Psyr_2719 at 3302.010 kb on - strand, within Psyr_2719 at 3302.281 kb on - strand at 3302.387 kb on + strand at 3302.388 kb on - strand at 3302.388 kb on - strand at 3302.704 kb on + strand, within Psyr_2720 at 3302.760 kb on - strand, within Psyr_2720 at 3302.762 kb on + strand, within Psyr_2720 at 3302.762 kb on + strand, within Psyr_2720 at 3302.762 kb on + strand, within Psyr_2720 at 3302.762 kb on + strand, within Psyr_2720 at 3302.762 kb on + strand, within Psyr_2720 at 3302.763 kb on - strand, within Psyr_2720 at 3302.763 kb on - strand, within Psyr_2720 at 3302.763 kb on - strand, within Psyr_2720 at 3302.763 kb on - strand, within Psyr_2720 at 3302.763 kb on - strand, within Psyr_2720 at 3302.786 kb on + strand, within Psyr_2720 at 3302.786 kb on + strand, within Psyr_2720 at 3302.786 kb on + strand, within Psyr_2720 at 3302.786 kb on + strand, within Psyr_2720 at 3302.786 kb on + strand, within Psyr_2720 at 3302.787 kb on - strand, within Psyr_2720 at 3302.787 kb on - strand, within Psyr_2720 at 3302.892 kb on + strand, within Psyr_2720 at 3302.927 kb on + strand, within Psyr_2720 at 3302.927 kb on + strand, within Psyr_2720 at 3302.927 kb on + strand, within Psyr_2720 at 3302.927 kb on + strand, within Psyr_2720 at 3302.928 kb on - strand, within Psyr_2720 at 3302.928 kb on - strand, within Psyr_2720 at 3302.984 kb on + strand, within Psyr_2720 at 3302.984 kb on + strand, within Psyr_2720 at 3302.984 kb on + strand, within Psyr_2720 at 3302.984 kb on + strand, within Psyr_2720 at 3302.984 kb on + strand, within Psyr_2720 at 3302.985 kb on - strand, within Psyr_2720 at 3303.117 kb on + strand, within Psyr_2720 at 3303.118 kb on - strand, within Psyr_2720 at 3303.118 kb on - strand, within Psyr_2720 at 3303.216 kb on - strand, within Psyr_2720 at 3303.254 kb on + strand at 3303.445 kb on + strand, within Psyr_2721 at 3303.446 kb on - strand, within Psyr_2721 at 3303.541 kb on + strand, within Psyr_2721 at 3303.541 kb on + strand, within Psyr_2721 at 3303.541 kb on + strand, within Psyr_2721 at 3303.598 kb on + strand, within Psyr_2721 at 3303.598 kb on + strand, within Psyr_2721 at 3303.599 kb on - strand, within Psyr_2721 at 3303.599 kb on - strand, within Psyr_2721 at 3303.599 kb on - strand, within Psyr_2721 at 3303.665 kb on - strand, within Psyr_2721 at 3303.800 kb on + strand, within Psyr_2721 at 3303.817 kb on + strand, within Psyr_2721 at 3303.818 kb on - strand, within Psyr_2721 at 3303.895 kb on + strand, within Psyr_2721 at 3303.896 kb on - strand, within Psyr_2721 at 3303.896 kb on - strand, within Psyr_2721 at 3303.896 kb on - strand, within Psyr_2721 at 3303.932 kb on + strand, within Psyr_2721 at 3304.121 kb on + strand at 3304.121 kb on + strand at 3304.121 kb on + strand at 3304.121 kb on + strand at 3304.122 kb on - strand at 3304.122 kb on - strand at 3304.122 kb on - strand at 3304.122 kb on - strand at 3304.122 kb on - strand at 3304.122 kb on - strand at 3304.122 kb on - strand at 3304.213 kb on + strand at 3304.214 kb on - strand at 3304.272 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,301,625 - -1.0 3,301,699 + +3.8 3,301,700 - +1.1 3,301,700 - -1.1 3,301,765 + -1.8 3,301,766 - -0.9 3,301,766 - -1.9 3,301,838 + Psyr_2719 0.14 +3.0 3,301,839 - Psyr_2719 0.14 +3.1 3,301,899 - Psyr_2719 0.24 -1.1 3,301,899 - Psyr_2719 0.24 -0.5 3,302,006 + Psyr_2719 0.43 +3.1 3,302,009 + Psyr_2719 0.43 +3.0 3,302,009 + Psyr_2719 0.43 +4.1 3,302,009 + Psyr_2719 0.43 +2.8 3,302,009 + Psyr_2719 0.43 +3.4 3,302,009 + Psyr_2719 0.43 +1.4 3,302,010 - Psyr_2719 0.43 +2.4 3,302,010 - Psyr_2719 0.43 +3.6 3,302,281 - -1.3 3,302,387 + -2.7 3,302,388 - -1.5 3,302,388 - +0.4 3,302,704 + Psyr_2720 0.37 +4.2 3,302,760 - Psyr_2720 0.43 +3.1 3,302,762 + Psyr_2720 0.43 +2.4 3,302,762 + Psyr_2720 0.43 -0.0 3,302,762 + Psyr_2720 0.43 +2.2 3,302,762 + Psyr_2720 0.43 -4.8 3,302,762 + Psyr_2720 0.43 -1.8 3,302,763 - Psyr_2720 0.43 +1.4 3,302,763 - Psyr_2720 0.43 -0.7 3,302,763 - Psyr_2720 0.43 +3.0 3,302,763 - Psyr_2720 0.43 +3.1 3,302,763 - Psyr_2720 0.43 -1.2 3,302,786 + Psyr_2720 0.45 -1.0 3,302,786 + Psyr_2720 0.45 +3.3 3,302,786 + Psyr_2720 0.45 +2.6 3,302,786 + Psyr_2720 0.45 -0.4 3,302,786 + Psyr_2720 0.45 +3.4 3,302,787 - Psyr_2720 0.45 +4.3 3,302,787 - Psyr_2720 0.45 +3.0 3,302,892 + Psyr_2720 0.56 -2.0 3,302,927 + Psyr_2720 0.60 +0.8 3,302,927 + Psyr_2720 0.60 -0.1 3,302,927 + Psyr_2720 0.60 -1.1 3,302,927 + Psyr_2720 0.60 -0.9 3,302,928 - Psyr_2720 0.60 +1.0 3,302,928 - Psyr_2720 0.60 +1.8 3,302,984 + Psyr_2720 0.66 +1.0 3,302,984 + Psyr_2720 0.66 -1.0 3,302,984 + Psyr_2720 0.66 -0.7 3,302,984 + Psyr_2720 0.66 -0.0 3,302,984 + Psyr_2720 0.66 -2.1 3,302,985 - Psyr_2720 0.66 +3.5 3,303,117 + Psyr_2720 0.79 -0.2 3,303,118 - Psyr_2720 0.79 +3.3 3,303,118 - Psyr_2720 0.79 +3.7 3,303,216 - Psyr_2720 0.89 -0.9 3,303,254 + +3.1 3,303,445 + Psyr_2721 0.15 +1.8 3,303,446 - Psyr_2721 0.15 +1.4 3,303,541 + Psyr_2721 0.26 +4.0 3,303,541 + Psyr_2721 0.26 +0.8 3,303,541 + Psyr_2721 0.26 -0.2 3,303,598 + Psyr_2721 0.32 +0.1 3,303,598 + Psyr_2721 0.32 -0.8 3,303,599 - Psyr_2721 0.33 -1.0 3,303,599 - Psyr_2721 0.33 +3.8 3,303,599 - Psyr_2721 0.33 +3.3 3,303,665 - Psyr_2721 0.40 -2.3 3,303,800 + Psyr_2721 0.56 -1.3 3,303,817 + Psyr_2721 0.58 -1.8 3,303,818 - Psyr_2721 0.58 +2.8 3,303,895 + Psyr_2721 0.66 -0.8 3,303,896 - Psyr_2721 0.67 +1.2 3,303,896 - Psyr_2721 0.67 -0.2 3,303,896 - Psyr_2721 0.67 -0.2 3,303,932 + Psyr_2721 0.71 +3.4 3,304,121 + +1.8 3,304,121 + -2.8 3,304,121 + +0.5 3,304,121 + -0.2 3,304,122 - -2.6 3,304,122 - -0.2 3,304,122 - -1.6 3,304,122 - +3.8 3,304,122 - +1.4 3,304,122 - -0.5 3,304,122 - -0.7 3,304,213 + +3.5 3,304,214 - +1.8 3,304,272 - -2.2
Or see this region's nucleotide sequence