Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2705 and Psyr_2706 are separated by 64 nucleotides Psyr_2706 and Psyr_2707 are separated by 127 nucleotides Psyr_2707 and Psyr_2708 are separated by 145 nucleotides
Psyr_2705: Psyr_2705 - Helix-turn-helix motif protein, at 3,286,524 to 3,287,135
_2705
Psyr_2706: Psyr_2706 - gamma-glutamyl-gamma-aminobutyrate hydrolase, at 3,287,200 to 3,287,949
_2706
Psyr_2707: Psyr_2707 - transcriptional regulator, LysR family, at 3,288,077 to 3,288,964
_2707
Psyr_2708: Psyr_2708 - Rieske [2Fe-2S] region, at 3,289,110 to 3,290,174
_2708
Position (kb)
3288
3289 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3287.081 kb on + strand at 3287.081 kb on + strand at 3287.081 kb on + strand at 3287.082 kb on - strand at 3287.082 kb on - strand at 3287.082 kb on - strand at 3287.161 kb on + strand at 3287.161 kb on + strand at 3287.162 kb on - strand at 3287.219 kb on + strand at 3287.219 kb on + strand at 3287.219 kb on + strand at 3287.220 kb on - strand at 3287.501 kb on + strand, within Psyr_2706 at 3287.502 kb on - strand, within Psyr_2706 at 3287.502 kb on - strand, within Psyr_2706 at 3287.689 kb on + strand, within Psyr_2706 at 3287.689 kb on + strand, within Psyr_2706 at 3287.689 kb on + strand, within Psyr_2706 at 3287.689 kb on + strand, within Psyr_2706 at 3287.689 kb on + strand, within Psyr_2706 at 3287.690 kb on - strand, within Psyr_2706 at 3287.690 kb on - strand, within Psyr_2706 at 3287.690 kb on - strand, within Psyr_2706 at 3287.729 kb on + strand, within Psyr_2706 at 3287.729 kb on + strand, within Psyr_2706 at 3287.729 kb on + strand, within Psyr_2706 at 3287.730 kb on - strand, within Psyr_2706 at 3287.730 kb on - strand, within Psyr_2706 at 3287.730 kb on - strand, within Psyr_2706 at 3287.867 kb on + strand, within Psyr_2706 at 3287.867 kb on + strand, within Psyr_2706 at 3287.867 kb on + strand, within Psyr_2706 at 3287.867 kb on + strand, within Psyr_2706 at 3287.867 kb on + strand, within Psyr_2706 at 3287.868 kb on - strand, within Psyr_2706 at 3287.868 kb on - strand, within Psyr_2706 at 3287.868 kb on - strand, within Psyr_2706 at 3287.868 kb on - strand, within Psyr_2706 at 3287.894 kb on + strand at 3287.894 kb on + strand at 3287.894 kb on + strand at 3287.895 kb on - strand at 3287.895 kb on - strand at 3287.895 kb on - strand at 3287.895 kb on - strand at 3287.949 kb on + strand at 3287.949 kb on + strand at 3287.949 kb on + strand at 3287.950 kb on - strand at 3287.950 kb on - strand at 3287.950 kb on - strand at 3287.950 kb on - strand at 3288.174 kb on + strand, within Psyr_2707 at 3288.175 kb on - strand, within Psyr_2707 at 3288.175 kb on - strand at 3288.175 kb on - strand, within Psyr_2707 at 3288.255 kb on + strand, within Psyr_2707 at 3288.398 kb on - strand, within Psyr_2707 at 3288.398 kb on - strand, within Psyr_2707 at 3288.405 kb on + strand, within Psyr_2707 at 3288.405 kb on + strand, within Psyr_2707 at 3288.405 kb on + strand, within Psyr_2707 at 3288.405 kb on + strand, within Psyr_2707 at 3288.406 kb on - strand, within Psyr_2707 at 3288.408 kb on + strand, within Psyr_2707 at 3288.409 kb on - strand, within Psyr_2707 at 3288.437 kb on + strand, within Psyr_2707 at 3288.448 kb on + strand, within Psyr_2707 at 3288.449 kb on - strand, within Psyr_2707 at 3288.449 kb on - strand, within Psyr_2707 at 3288.526 kb on + strand, within Psyr_2707 at 3288.572 kb on + strand, within Psyr_2707 at 3288.572 kb on + strand, within Psyr_2707 at 3288.572 kb on + strand, within Psyr_2707 at 3288.706 kb on - strand, within Psyr_2707 at 3288.706 kb on - strand, within Psyr_2707 at 3288.706 kb on - strand, within Psyr_2707 at 3288.948 kb on + strand at 3289.016 kb on + strand at 3289.016 kb on + strand at 3289.017 kb on - strand at 3289.036 kb on + strand at 3289.132 kb on - strand at 3289.207 kb on - strand at 3289.281 kb on + strand, within Psyr_2708 at 3289.281 kb on + strand, within Psyr_2708 at 3289.281 kb on + strand, within Psyr_2708 at 3289.584 kb on + strand, within Psyr_2708 at 3289.584 kb on + strand, within Psyr_2708 at 3289.584 kb on + strand, within Psyr_2708 at 3289.584 kb on + strand, within Psyr_2708 at 3289.584 kb on + strand, within Psyr_2708 at 3289.585 kb on - strand, within Psyr_2708 at 3289.912 kb on - strand, within Psyr_2708 at 3289.912 kb on - strand, within Psyr_2708
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,287,081 + +1.8 3,287,081 + -1.9 3,287,081 + -2.1 3,287,082 - +2.6 3,287,082 - -1.6 3,287,082 - -3.1 3,287,161 + -3.1 3,287,161 + -1.8 3,287,162 - +2.1 3,287,219 + +4.2 3,287,219 + +1.8 3,287,219 + +3.8 3,287,220 - -1.0 3,287,501 + Psyr_2706 0.40 +2.4 3,287,502 - Psyr_2706 0.40 +2.1 3,287,502 - Psyr_2706 0.40 +1.8 3,287,689 + Psyr_2706 0.65 +0.8 3,287,689 + Psyr_2706 0.65 -0.2 3,287,689 + Psyr_2706 0.65 +3.6 3,287,689 + Psyr_2706 0.65 +2.1 3,287,689 + Psyr_2706 0.65 +2.6 3,287,690 - Psyr_2706 0.65 -1.3 3,287,690 - Psyr_2706 0.65 +0.5 3,287,690 - Psyr_2706 0.65 -3.2 3,287,729 + Psyr_2706 0.71 -0.2 3,287,729 + Psyr_2706 0.71 -4.2 3,287,729 + Psyr_2706 0.71 +1.8 3,287,730 - Psyr_2706 0.71 +3.6 3,287,730 - Psyr_2706 0.71 +2.4 3,287,730 - Psyr_2706 0.71 +1.4 3,287,867 + Psyr_2706 0.89 +1.8 3,287,867 + Psyr_2706 0.89 +3.0 3,287,867 + Psyr_2706 0.89 +0.7 3,287,867 + Psyr_2706 0.89 +1.4 3,287,867 + Psyr_2706 0.89 -0.8 3,287,868 - Psyr_2706 0.89 +2.6 3,287,868 - Psyr_2706 0.89 +0.4 3,287,868 - Psyr_2706 0.89 +2.1 3,287,868 - Psyr_2706 0.89 +3.6 3,287,894 + -2.0 3,287,894 + +0.8 3,287,894 + -3.7 3,287,895 - +0.3 3,287,895 - -0.9 3,287,895 - +0.1 3,287,895 - -2.6 3,287,949 + +3.1 3,287,949 + +1.4 3,287,949 + -1.6 3,287,950 - +0.8 3,287,950 - +0.1 3,287,950 - +0.5 3,287,950 - -3.2 3,288,174 + Psyr_2707 0.11 +2.8 3,288,175 - Psyr_2707 0.11 +2.6 3,288,175 - +2.8 3,288,175 - Psyr_2707 0.11 -1.3 3,288,255 + Psyr_2707 0.20 +0.5 3,288,398 - Psyr_2707 0.36 +0.7 3,288,398 - Psyr_2707 0.36 -3.6 3,288,405 + Psyr_2707 0.37 +0.8 3,288,405 + Psyr_2707 0.37 -0.1 3,288,405 + Psyr_2707 0.37 -1.4 3,288,405 + Psyr_2707 0.37 -0.7 3,288,406 - Psyr_2707 0.37 +3.1 3,288,408 + Psyr_2707 0.37 +2.1 3,288,409 - Psyr_2707 0.37 -1.0 3,288,437 + Psyr_2707 0.41 +4.1 3,288,448 + Psyr_2707 0.42 +2.6 3,288,449 - Psyr_2707 0.42 -1.1 3,288,449 - Psyr_2707 0.42 +3.9 3,288,526 + Psyr_2707 0.51 +3.0 3,288,572 + Psyr_2707 0.56 +1.4 3,288,572 + Psyr_2707 0.56 -1.2 3,288,572 + Psyr_2707 0.56 +0.8 3,288,706 - Psyr_2707 0.71 -2.3 3,288,706 - Psyr_2707 0.71 +2.6 3,288,706 - Psyr_2707 0.71 +3.3 3,288,948 + +2.8 3,289,016 + -1.6 3,289,016 + -0.6 3,289,017 - +2.1 3,289,036 + +1.4 3,289,132 - +0.8 3,289,207 - +2.6 3,289,281 + Psyr_2708 0.16 +1.4 3,289,281 + Psyr_2708 0.16 +0.6 3,289,281 + Psyr_2708 0.16 +0.2 3,289,584 + Psyr_2708 0.45 -1.2 3,289,584 + Psyr_2708 0.45 -1.9 3,289,584 + Psyr_2708 0.45 -1.0 3,289,584 + Psyr_2708 0.45 +1.2 3,289,584 + Psyr_2708 0.45 +1.4 3,289,585 - Psyr_2708 0.45 +3.0 3,289,912 - Psyr_2708 0.75 +3.3 3,289,912 - Psyr_2708 0.75 -0.6
Or see this region's nucleotide sequence