Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2641 and Psyr_2642 overlap by 4 nucleotides Psyr_2642 and Psyr_2643 are separated by 906 nucleotides
Psyr_2641: Psyr_2641 - hypothetical protein, at 3,209,021 to 3,209,428
_2641
Psyr_2642: Psyr_2642 - hypothetical protein, at 3,209,425 to 3,210,069
_2642
Psyr_2643: Psyr_2643 - hypothetical protein, at 3,210,976 to 3,212,205
_2643
Position (kb)
3209
3210
3211 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 3208.491 kb on + strand at 3208.491 kb on + strand at 3208.491 kb on + strand at 3208.491 kb on + strand at 3208.492 kb on - strand at 3208.625 kb on + strand at 3208.626 kb on - strand at 3208.626 kb on - strand at 3208.626 kb on - strand at 3208.626 kb on - strand at 3208.626 kb on - strand at 3208.662 kb on - strand at 3208.741 kb on + strand at 3208.742 kb on - strand at 3208.742 kb on - strand at 3208.766 kb on + strand at 3208.767 kb on - strand at 3208.864 kb on - strand at 3208.913 kb on + strand at 3208.913 kb on + strand at 3208.913 kb on + strand at 3208.917 kb on + strand at 3208.918 kb on - strand at 3208.922 kb on + strand at 3208.922 kb on + strand at 3208.923 kb on - strand at 3208.948 kb on + strand at 3208.972 kb on - strand at 3208.984 kb on + strand at 3209.139 kb on + strand, within Psyr_2641 at 3209.140 kb on - strand, within Psyr_2641 at 3209.140 kb on - strand, within Psyr_2641 at 3209.161 kb on + strand, within Psyr_2641 at 3209.161 kb on + strand, within Psyr_2641 at 3209.162 kb on - strand, within Psyr_2641 at 3209.300 kb on + strand, within Psyr_2641 at 3209.301 kb on - strand at 3209.304 kb on + strand, within Psyr_2641 at 3209.305 kb on - strand, within Psyr_2641 at 3209.358 kb on - strand, within Psyr_2641 at 3209.611 kb on + strand, within Psyr_2642 at 3209.615 kb on - strand, within Psyr_2642 at 3209.641 kb on + strand, within Psyr_2642 at 3209.641 kb on + strand, within Psyr_2642 at 3209.641 kb on + strand, within Psyr_2642 at 3209.641 kb on + strand, within Psyr_2642 at 3209.642 kb on - strand, within Psyr_2642 at 3209.642 kb on - strand, within Psyr_2642 at 3209.760 kb on + strand, within Psyr_2642 at 3209.760 kb on + strand, within Psyr_2642 at 3209.774 kb on + strand, within Psyr_2642 at 3209.774 kb on + strand, within Psyr_2642 at 3209.774 kb on + strand, within Psyr_2642 at 3209.775 kb on - strand, within Psyr_2642 at 3209.775 kb on - strand, within Psyr_2642 at 3209.775 kb on - strand, within Psyr_2642 at 3209.813 kb on + strand, within Psyr_2642 at 3209.884 kb on - strand, within Psyr_2642 at 3209.929 kb on + strand, within Psyr_2642 at 3210.072 kb on + strand at 3210.095 kb on - strand at 3210.115 kb on + strand at 3210.178 kb on - strand at 3210.260 kb on + strand at 3210.342 kb on + strand at 3210.431 kb on + strand at 3210.497 kb on + strand at 3210.542 kb on + strand at 3210.558 kb on + strand at 3210.562 kb on + strand at 3210.563 kb on - strand at 3210.565 kb on - strand at 3210.569 kb on + strand at 3210.570 kb on - strand at 3210.570 kb on - strand at 3210.693 kb on + strand at 3210.703 kb on + strand at 3210.704 kb on - strand at 3210.723 kb on + strand at 3210.810 kb on + strand at 3210.811 kb on - strand at 3210.889 kb on - strand at 3210.953 kb on - strand at 3210.953 kb on - strand at 3211.024 kb on + strand at 3211.025 kb on - strand at 3211.066 kb on + strand at 3211.066 kb on + strand at 3211.067 kb on - strand at 3211.069 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,208,491 + -3.4 3,208,491 + -4.7 3,208,491 + -0.3 3,208,491 + -1.2 3,208,492 - +3.0 3,208,625 + +1.8 3,208,626 - -0.2 3,208,626 - -3.7 3,208,626 - +0.8 3,208,626 - -1.8 3,208,626 - -2.0 3,208,662 - +2.6 3,208,741 + -0.2 3,208,742 - +2.4 3,208,742 - -0.2 3,208,766 + -4.1 3,208,767 - -2.4 3,208,864 - +1.4 3,208,913 + -0.2 3,208,913 + +0.8 3,208,913 + -5.4 3,208,917 + -0.2 3,208,918 - +1.4 3,208,922 + -0.2 3,208,922 + -0.2 3,208,923 - -0.2 3,208,948 + +1.4 3,208,972 - -1.3 3,208,984 + -0.2 3,209,139 + Psyr_2641 0.29 -4.1 3,209,140 - Psyr_2641 0.29 +1.4 3,209,140 - Psyr_2641 0.29 -0.2 3,209,161 + Psyr_2641 0.34 -0.2 3,209,161 + Psyr_2641 0.34 -2.4 3,209,162 - Psyr_2641 0.35 +0.8 3,209,300 + Psyr_2641 0.68 -2.9 3,209,301 - +2.1 3,209,304 + Psyr_2641 0.69 -4.1 3,209,305 - Psyr_2641 0.70 +3.8 3,209,358 - Psyr_2641 0.83 -2.7 3,209,611 + Psyr_2642 0.29 +3.3 3,209,615 - Psyr_2642 0.29 +2.6 3,209,641 + Psyr_2642 0.33 -0.2 3,209,641 + Psyr_2642 0.33 -3.8 3,209,641 + Psyr_2642 0.33 -3.2 3,209,641 + Psyr_2642 0.33 +0.8 3,209,642 - Psyr_2642 0.34 +2.4 3,209,642 - Psyr_2642 0.34 -1.8 3,209,760 + Psyr_2642 0.52 -4.6 3,209,760 + Psyr_2642 0.52 -1.6 3,209,774 + Psyr_2642 0.54 +1.4 3,209,774 + Psyr_2642 0.54 +0.8 3,209,774 + Psyr_2642 0.54 -2.1 3,209,775 - Psyr_2642 0.54 -1.7 3,209,775 - Psyr_2642 0.54 +1.8 3,209,775 - Psyr_2642 0.54 +1.4 3,209,813 + Psyr_2642 0.60 -2.2 3,209,884 - Psyr_2642 0.71 +2.8 3,209,929 + Psyr_2642 0.78 -3.6 3,210,072 + -3.5 3,210,095 - +2.4 3,210,115 + -2.8 3,210,178 - -2.7 3,210,260 + -2.7 3,210,342 + -2.6 3,210,431 + +1.4 3,210,497 + -3.6 3,210,542 + -0.2 3,210,558 + +2.1 3,210,562 + +0.2 3,210,563 - -3.0 3,210,565 - -0.9 3,210,569 + -0.2 3,210,570 - -2.8 3,210,570 - -3.1 3,210,693 + -5.1 3,210,703 + -3.9 3,210,704 - -1.5 3,210,723 + -2.5 3,210,810 + -1.1 3,210,811 - -1.5 3,210,889 - +3.1 3,210,953 - +2.8 3,210,953 - -1.3 3,211,024 + -0.2 3,211,025 - -1.8 3,211,066 + +0.8 3,211,066 + +0.8 3,211,067 - +3.3 3,211,069 + +1.4
Or see this region's nucleotide sequence