Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2593 and Psyr_2594 overlap by 4 nucleotides Psyr_2594 and Psyr_2595 overlap by 4 nucleotides Psyr_2595 and Psyr_2596 are separated by 156 nucleotides
Psyr_2593: Psyr_2593 - ABC transporter, at 3,039,718 to 3,040,509
_2593
Psyr_2594: Psyr_2594 - unknown conserved protein in bacilli, at 3,040,506 to 3,041,285
_2594
Psyr_2595: Psyr_2595 - Dimethylmenaquinone methyltransferase, at 3,041,282 to 3,041,926
_2595
Psyr_2596: Psyr_2596 - PAS, at 3,042,083 to 3,043,444
_2596
Position (kb)
3041
3042 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 3040.867 kb on - strand, within Psyr_2594 at 3041.002 kb on + strand, within Psyr_2594 at 3041.010 kb on + strand, within Psyr_2594 at 3041.127 kb on + strand, within Psyr_2594 at 3041.215 kb on - strand at 3041.217 kb on + strand at 3041.340 kb on - strand at 3041.514 kb on + strand, within Psyr_2595 at 3041.515 kb on - strand, within Psyr_2595 at 3041.515 kb on - strand, within Psyr_2595 at 3041.515 kb on - strand, within Psyr_2595 at 3041.734 kb on + strand, within Psyr_2595 at 3041.736 kb on + strand, within Psyr_2595 at 3041.736 kb on + strand, within Psyr_2595 at 3041.736 kb on + strand, within Psyr_2595 at 3041.737 kb on - strand, within Psyr_2595 at 3041.737 kb on - strand, within Psyr_2595 at 3041.765 kb on - strand, within Psyr_2595 at 3041.790 kb on + strand, within Psyr_2595 at 3041.794 kb on + strand, within Psyr_2595 at 3041.795 kb on - strand, within Psyr_2595 at 3041.795 kb on - strand, within Psyr_2595 at 3041.991 kb on - strand at 3042.011 kb on - strand at 3042.046 kb on + strand at 3042.049 kb on + strand at 3042.157 kb on + strand at 3042.253 kb on - strand, within Psyr_2596 at 3042.253 kb on - strand, within Psyr_2596 at 3042.290 kb on + strand, within Psyr_2596 at 3042.291 kb on - strand, within Psyr_2596 at 3042.473 kb on - strand, within Psyr_2596 at 3042.688 kb on + strand, within Psyr_2596 at 3042.688 kb on + strand, within Psyr_2596 at 3042.688 kb on + strand, within Psyr_2596 at 3042.688 kb on + strand, within Psyr_2596 at 3042.689 kb on - strand, within Psyr_2596 at 3042.689 kb on - strand, within Psyr_2596 at 3042.689 kb on - strand, within Psyr_2596 at 3042.689 kb on - strand, within Psyr_2596 at 3042.689 kb on - strand, within Psyr_2596 at 3042.689 kb on - strand, within Psyr_2596 at 3042.728 kb on + strand, within Psyr_2596 at 3042.729 kb on - strand, within Psyr_2596 at 3042.729 kb on - strand, within Psyr_2596 at 3042.729 kb on - strand, within Psyr_2596 at 3042.729 kb on - strand, within Psyr_2596 at 3042.729 kb on - strand, within Psyr_2596 at 3042.729 kb on - strand, within Psyr_2596 at 3042.766 kb on + strand, within Psyr_2596 at 3042.810 kb on + strand, within Psyr_2596 at 3042.810 kb on + strand, within Psyr_2596 at 3042.810 kb on + strand, within Psyr_2596 at 3042.810 kb on + strand, within Psyr_2596 at 3042.810 kb on + strand, within Psyr_2596 at 3042.810 kb on + strand, within Psyr_2596 at 3042.810 kb on + strand, within Psyr_2596 at 3042.810 kb on + strand, within Psyr_2596 at 3042.810 kb on + strand, within Psyr_2596 at 3042.811 kb on - strand, within Psyr_2596 at 3042.811 kb on - strand, within Psyr_2596 at 3042.811 kb on - strand, within Psyr_2596 at 3042.811 kb on - strand, within Psyr_2596 at 3042.811 kb on - strand, within Psyr_2596 at 3042.811 kb on - strand, within Psyr_2596 at 3042.811 kb on - strand, within Psyr_2596 at 3042.811 kb on - strand, within Psyr_2596 at 3042.811 kb on - strand, within Psyr_2596 at 3042.862 kb on + strand, within Psyr_2596 at 3042.863 kb on - strand, within Psyr_2596
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,040,867 - Psyr_2594 0.46 -1.8 3,041,002 + Psyr_2594 0.64 -1.3 3,041,010 + Psyr_2594 0.65 +0.3 3,041,127 + Psyr_2594 0.80 -1.2 3,041,215 - +3.6 3,041,217 + -2.5 3,041,340 - +3.4 3,041,514 + Psyr_2595 0.36 -1.8 3,041,515 - Psyr_2595 0.36 -1.7 3,041,515 - Psyr_2595 0.36 +2.6 3,041,515 - Psyr_2595 0.36 -1.0 3,041,734 + Psyr_2595 0.70 +1.4 3,041,736 + Psyr_2595 0.70 +1.4 3,041,736 + Psyr_2595 0.70 -0.2 3,041,736 + Psyr_2595 0.70 -4.2 3,041,737 - Psyr_2595 0.71 -0.1 3,041,737 - Psyr_2595 0.71 +2.8 3,041,765 - Psyr_2595 0.75 -1.9 3,041,790 + Psyr_2595 0.79 -1.2 3,041,794 + Psyr_2595 0.79 -2.2 3,041,795 - Psyr_2595 0.80 -4.2 3,041,795 - Psyr_2595 0.80 -4.2 3,041,991 - -0.2 3,042,011 - +1.4 3,042,046 + +1.8 3,042,049 + +1.8 3,042,157 + -2.3 3,042,253 - Psyr_2596 0.12 +1.8 3,042,253 - Psyr_2596 0.12 +0.8 3,042,290 + Psyr_2596 0.15 +0.4 3,042,291 - Psyr_2596 0.15 +2.6 3,042,473 - Psyr_2596 0.29 -2.4 3,042,688 + Psyr_2596 0.44 +4.1 3,042,688 + Psyr_2596 0.44 -0.6 3,042,688 + Psyr_2596 0.44 +3.8 3,042,688 + Psyr_2596 0.44 +2.4 3,042,689 - Psyr_2596 0.44 +3.0 3,042,689 - Psyr_2596 0.44 +0.8 3,042,689 - Psyr_2596 0.44 -2.4 3,042,689 - Psyr_2596 0.44 +0.8 3,042,689 - Psyr_2596 0.44 -5.4 3,042,689 - Psyr_2596 0.44 -1.3 3,042,728 + Psyr_2596 0.47 +2.3 3,042,729 - Psyr_2596 0.47 +2.6 3,042,729 - Psyr_2596 0.47 +2.1 3,042,729 - Psyr_2596 0.47 +0.8 3,042,729 - Psyr_2596 0.47 +2.6 3,042,729 - Psyr_2596 0.47 -1.2 3,042,729 - Psyr_2596 0.47 +1.4 3,042,766 + Psyr_2596 0.50 +2.6 3,042,810 + Psyr_2596 0.53 -1.5 3,042,810 + Psyr_2596 0.53 +1.4 3,042,810 + Psyr_2596 0.53 -0.2 3,042,810 + Psyr_2596 0.53 +3.6 3,042,810 + Psyr_2596 0.53 +2.4 3,042,810 + Psyr_2596 0.53 -0.2 3,042,810 + Psyr_2596 0.53 +3.3 3,042,810 + Psyr_2596 0.53 +3.9 3,042,810 + Psyr_2596 0.53 +3.4 3,042,811 - Psyr_2596 0.53 +1.8 3,042,811 - Psyr_2596 0.53 -0.2 3,042,811 - Psyr_2596 0.53 -1.4 3,042,811 - Psyr_2596 0.53 -0.6 3,042,811 - Psyr_2596 0.53 -2.1 3,042,811 - Psyr_2596 0.53 +1.8 3,042,811 - Psyr_2596 0.53 -2.9 3,042,811 - Psyr_2596 0.53 -0.5 3,042,811 - Psyr_2596 0.53 -1.7 3,042,862 + Psyr_2596 0.57 +1.4 3,042,863 - Psyr_2596 0.57 +2.8
Or see this region's nucleotide sequence