Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2549 and Psyr_2550 overlap by 4 nucleotides Psyr_2550 and Psyr_2551 overlap by 4 nucleotides Psyr_2551 and Psyr_2552 are separated by 12 nucleotides Psyr_2552 and Psyr_2553 are separated by 1 nucleotides
Psyr_2549: Psyr_2549 - hypothetical protein, at 2,963,117 to 2,964,187
_2549
Psyr_2550: Psyr_2550 - hypothetical protein, at 2,964,184 to 2,964,738
_2550
Psyr_2551: Psyr_2551 - acetylornithine aminotransferase apoenzyme, at 2,964,735 to 2,965,991
_2551
Psyr_2552: Psyr_2552 - cyclase/dehydrase, at 2,966,004 to 2,966,471
_2552
Psyr_2553: Psyr_2553 - Putative dehydrogenase domain of multifunctional non-ribosomal peptide synthetases-related enzyme, at 2,966,473 to 2,967,582
_2553
Position (kb)
2964
2965
2966 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 2963.934 kb on - strand, within Psyr_2549 at 2964.107 kb on + strand at 2964.107 kb on + strand at 2964.107 kb on + strand at 2964.166 kb on + strand at 2964.245 kb on + strand, within Psyr_2550 at 2964.245 kb on + strand, within Psyr_2550 at 2964.245 kb on + strand, within Psyr_2550 at 2964.245 kb on + strand, within Psyr_2550 at 2964.245 kb on + strand, within Psyr_2550 at 2964.245 kb on + strand, within Psyr_2550 at 2964.246 kb on - strand, within Psyr_2550 at 2964.246 kb on - strand, within Psyr_2550 at 2964.246 kb on - strand, within Psyr_2550 at 2964.246 kb on - strand, within Psyr_2550 at 2964.246 kb on - strand, within Psyr_2550 at 2964.246 kb on - strand, within Psyr_2550 at 2964.246 kb on - strand, within Psyr_2550 at 2964.317 kb on - strand, within Psyr_2550 at 2964.317 kb on - strand, within Psyr_2550 at 2964.317 kb on - strand, within Psyr_2550 at 2964.735 kb on - strand at 2964.802 kb on - strand at 2964.833 kb on + strand at 2964.834 kb on - strand at 2965.095 kb on + strand, within Psyr_2551 at 2965.096 kb on - strand, within Psyr_2551 at 2965.224 kb on + strand, within Psyr_2551 at 2965.224 kb on + strand, within Psyr_2551 at 2965.225 kb on - strand, within Psyr_2551 at 2965.561 kb on - strand, within Psyr_2551 at 2965.618 kb on - strand, within Psyr_2551 at 2965.815 kb on + strand, within Psyr_2551 at 2965.815 kb on + strand, within Psyr_2551 at 2965.816 kb on - strand, within Psyr_2551 at 2965.816 kb on - strand, within Psyr_2551 at 2965.816 kb on - strand, within Psyr_2551 at 2965.816 kb on - strand, within Psyr_2551 at 2965.816 kb on - strand, within Psyr_2551 at 2965.899 kb on + strand at 2965.900 kb on - strand at 2966.015 kb on - strand at 2966.015 kb on - strand at 2966.142 kb on + strand, within Psyr_2552 at 2966.143 kb on - strand, within Psyr_2552 at 2966.143 kb on - strand, within Psyr_2552 at 2966.153 kb on + strand, within Psyr_2552 at 2966.154 kb on - strand, within Psyr_2552 at 2966.154 kb on - strand, within Psyr_2552 at 2966.848 kb on + strand, within Psyr_2553 at 2966.848 kb on + strand, within Psyr_2553 at 2966.849 kb on - strand, within Psyr_2553 at 2966.849 kb on - strand, within Psyr_2553 at 2966.849 kb on - strand, within Psyr_2553 at 2966.899 kb on + strand, within Psyr_2553
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,963,934 - Psyr_2549 0.76 -0.6 2,964,107 + +1.0 2,964,107 + +2.4 2,964,107 + +3.5 2,964,166 + +3.4 2,964,245 + Psyr_2550 0.11 -0.3 2,964,245 + Psyr_2550 0.11 +2.4 2,964,245 + Psyr_2550 0.11 -0.9 2,964,245 + Psyr_2550 0.11 -1.2 2,964,245 + Psyr_2550 0.11 -0.2 2,964,245 + Psyr_2550 0.11 +0.1 2,964,246 - Psyr_2550 0.11 +2.1 2,964,246 - Psyr_2550 0.11 +4.8 2,964,246 - Psyr_2550 0.11 +3.3 2,964,246 - Psyr_2550 0.11 +4.6 2,964,246 - Psyr_2550 0.11 -1.7 2,964,246 - Psyr_2550 0.11 -1.0 2,964,246 - Psyr_2550 0.11 +1.1 2,964,317 - Psyr_2550 0.24 +2.4 2,964,317 - Psyr_2550 0.24 -1.9 2,964,317 - Psyr_2550 0.24 -0.7 2,964,735 - +0.0 2,964,802 - -2.1 2,964,833 + +0.8 2,964,834 - +3.4 2,965,095 + Psyr_2551 0.29 -0.4 2,965,096 - Psyr_2551 0.29 -0.9 2,965,224 + Psyr_2551 0.39 +2.6 2,965,224 + Psyr_2551 0.39 +3.5 2,965,225 - Psyr_2551 0.39 +2.1 2,965,561 - Psyr_2551 0.66 -4.3 2,965,618 - Psyr_2551 0.70 +1.4 2,965,815 + Psyr_2551 0.86 +2.4 2,965,815 + Psyr_2551 0.86 +2.4 2,965,816 - Psyr_2551 0.86 +1.4 2,965,816 - Psyr_2551 0.86 +4.4 2,965,816 - Psyr_2551 0.86 -2.3 2,965,816 - Psyr_2551 0.86 +2.4 2,965,816 - Psyr_2551 0.86 +3.5 2,965,899 + +3.3 2,965,900 - -0.5 2,966,015 - +2.4 2,966,015 - -1.6 2,966,142 + Psyr_2552 0.29 -1.2 2,966,143 - Psyr_2552 0.30 +2.1 2,966,143 - Psyr_2552 0.30 +0.4 2,966,153 + Psyr_2552 0.32 -1.7 2,966,154 - Psyr_2552 0.32 +1.4 2,966,154 - Psyr_2552 0.32 -0.6 2,966,848 + Psyr_2553 0.34 +1.0 2,966,848 + Psyr_2553 0.34 +0.8 2,966,849 - Psyr_2553 0.34 +3.6 2,966,849 - Psyr_2553 0.34 +0.6 2,966,849 - Psyr_2553 0.34 +2.6 2,966,899 + Psyr_2553 0.38 +2.4
Or see this region's nucleotide sequence