Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2534 and Psyr_2535 are separated by 409 nucleotides Psyr_2535 and Psyr_2536 are separated by 68 nucleotides
Psyr_2534: Psyr_2534 - Carboxymethylenebutenolidase, at 2,948,270 to 2,949,157
_2534
Psyr_2535: Psyr_2535 - GGDEF domain protein, at 2,949,567 to 2,950,619
_2535
Psyr_2536: Psyr_2536 - transcriptional regulator, LysR family, at 2,950,688 to 2,951,677
_2536
Position (kb)
2949
2950
2951 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2948.943 kb on + strand, within Psyr_2534 at 2948.943 kb on + strand, within Psyr_2534 at 2948.954 kb on - strand, within Psyr_2534 at 2948.995 kb on - strand, within Psyr_2534 at 2948.995 kb on - strand, within Psyr_2534 at 2949.099 kb on + strand at 2949.102 kb on + strand at 2949.103 kb on - strand at 2949.103 kb on - strand at 2949.108 kb on + strand at 2949.108 kb on + strand at 2949.109 kb on - strand at 2949.109 kb on - strand at 2949.217 kb on + strand at 2949.217 kb on + strand at 2949.217 kb on + strand at 2949.217 kb on + strand at 2949.218 kb on - strand at 2949.218 kb on - strand at 2949.218 kb on - strand at 2949.218 kb on - strand at 2949.246 kb on + strand at 2949.249 kb on + strand at 2949.250 kb on - strand at 2949.277 kb on + strand at 2949.278 kb on - strand at 2949.458 kb on + strand at 2949.458 kb on + strand at 2949.458 kb on + strand at 2949.458 kb on + strand at 2949.458 kb on + strand at 2949.458 kb on + strand at 2949.458 kb on + strand at 2949.458 kb on + strand at 2949.458 kb on + strand at 2949.458 kb on + strand at 2949.459 kb on - strand at 2949.459 kb on - strand at 2949.459 kb on - strand at 2949.459 kb on - strand at 2949.459 kb on - strand at 2949.459 kb on - strand at 2949.475 kb on - strand at 2949.477 kb on - strand at 2949.503 kb on + strand at 2949.503 kb on + strand at 2949.504 kb on - strand at 2949.504 kb on - strand at 2949.504 kb on - strand at 2949.537 kb on - strand at 2949.537 kb on - strand at 2949.745 kb on - strand, within Psyr_2535 at 2949.759 kb on + strand, within Psyr_2535 at 2949.760 kb on - strand, within Psyr_2535 at 2949.760 kb on - strand, within Psyr_2535 at 2949.873 kb on + strand, within Psyr_2535 at 2949.874 kb on - strand, within Psyr_2535 at 2949.878 kb on - strand, within Psyr_2535 at 2949.878 kb on - strand, within Psyr_2535 at 2950.051 kb on + strand, within Psyr_2535 at 2950.052 kb on - strand, within Psyr_2535 at 2950.052 kb on - strand, within Psyr_2535 at 2950.097 kb on - strand at 2950.437 kb on - strand, within Psyr_2535 at 2950.437 kb on - strand, within Psyr_2535 at 2950.572 kb on + strand at 2950.572 kb on + strand at 2950.572 kb on + strand at 2950.572 kb on + strand at 2950.572 kb on + strand at 2950.572 kb on + strand at 2950.572 kb on + strand at 2950.572 kb on + strand at 2950.572 kb on + strand at 2950.573 kb on - strand at 2950.573 kb on - strand at 2950.573 kb on - strand at 2950.573 kb on - strand at 2950.573 kb on - strand at 2950.573 kb on - strand at 2950.575 kb on - strand at 2950.652 kb on + strand at 2950.726 kb on + strand at 2950.919 kb on + strand, within Psyr_2536 at 2950.919 kb on + strand, within Psyr_2536 at 2950.920 kb on - strand, within Psyr_2536 at 2950.920 kb on - strand, within Psyr_2536 at 2950.920 kb on - strand, within Psyr_2536 at 2950.920 kb on - strand, within Psyr_2536 at 2951.392 kb on + strand, within Psyr_2536 at 2951.392 kb on + strand, within Psyr_2536 at 2951.392 kb on + strand, within Psyr_2536 at 2951.392 kb on + strand, within Psyr_2536 at 2951.392 kb on + strand, within Psyr_2536 at 2951.393 kb on - strand, within Psyr_2536
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,948,943 + Psyr_2534 0.76 +2.1 2,948,943 + Psyr_2534 0.76 -0.4 2,948,954 - Psyr_2534 0.77 +3.1 2,948,995 - Psyr_2534 0.82 -1.8 2,948,995 - Psyr_2534 0.82 -2.1 2,949,099 + -3.0 2,949,102 + -1.8 2,949,103 - +2.6 2,949,103 - -2.6 2,949,108 + +1.4 2,949,108 + +1.8 2,949,109 - +2.8 2,949,109 - +3.7 2,949,217 + -0.3 2,949,217 + -1.6 2,949,217 + +3.3 2,949,217 + -2.9 2,949,218 - -2.0 2,949,218 - +2.4 2,949,218 - +1.4 2,949,218 - +2.4 2,949,246 + -1.4 2,949,249 + -1.9 2,949,250 - -1.1 2,949,277 + +1.4 2,949,278 - -3.2 2,949,458 + +1.8 2,949,458 + -4.1 2,949,458 + +0.8 2,949,458 + -2.2 2,949,458 + -4.3 2,949,458 + +2.3 2,949,458 + +0.8 2,949,458 + +1.8 2,949,458 + +1.4 2,949,458 + +0.8 2,949,459 - -1.1 2,949,459 - +2.8 2,949,459 - -0.7 2,949,459 - +3.1 2,949,459 - -1.9 2,949,459 - +3.0 2,949,475 - +2.6 2,949,477 - +1.8 2,949,503 + +3.5 2,949,503 + -1.6 2,949,504 - +0.3 2,949,504 - +3.5 2,949,504 - +3.0 2,949,537 - +3.1 2,949,537 - -0.9 2,949,745 - Psyr_2535 0.17 +1.8 2,949,759 + Psyr_2535 0.18 -0.2 2,949,760 - Psyr_2535 0.18 -1.1 2,949,760 - Psyr_2535 0.18 +1.4 2,949,873 + Psyr_2535 0.29 -0.2 2,949,874 - Psyr_2535 0.29 -0.2 2,949,878 - Psyr_2535 0.30 +0.1 2,949,878 - Psyr_2535 0.30 -0.6 2,950,051 + Psyr_2535 0.46 -1.1 2,950,052 - Psyr_2535 0.46 -1.7 2,950,052 - Psyr_2535 0.46 +2.4 2,950,097 - +1.8 2,950,437 - Psyr_2535 0.83 +1.8 2,950,437 - Psyr_2535 0.83 -1.7 2,950,572 + +3.5 2,950,572 + -0.7 2,950,572 + -1.6 2,950,572 + +2.6 2,950,572 + +3.0 2,950,572 + -3.2 2,950,572 + +0.5 2,950,572 + +0.8 2,950,572 + +3.3 2,950,573 - +0.3 2,950,573 - -1.6 2,950,573 - +1.4 2,950,573 - +3.0 2,950,573 - -1.7 2,950,573 - -1.4 2,950,575 - -2.1 2,950,652 + +1.8 2,950,726 + +2.1 2,950,919 + Psyr_2536 0.23 -0.3 2,950,919 + Psyr_2536 0.23 -2.0 2,950,920 - Psyr_2536 0.23 -2.4 2,950,920 - Psyr_2536 0.23 -1.9 2,950,920 - Psyr_2536 0.23 -0.9 2,950,920 - Psyr_2536 0.23 -2.0 2,951,392 + Psyr_2536 0.71 +3.0 2,951,392 + Psyr_2536 0.71 +3.0 2,951,392 + Psyr_2536 0.71 +3.3 2,951,392 + Psyr_2536 0.71 -1.3 2,951,392 + Psyr_2536 0.71 +3.7 2,951,393 - Psyr_2536 0.71 +2.8
Or see this region's nucleotide sequence