Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2519 and Psyr_2520 are separated by 204 nucleotides Psyr_2520 and Psyr_2521 overlap by 4 nucleotides Psyr_2521 and Psyr_2522 are separated by 7 nucleotides
Psyr_2519: Psyr_2519 - Aldo/keto reductase, at 2,927,785 to 2,928,780
_2519
Psyr_2520: Psyr_2520 - SOS-response transcriptional repressor, LexA, at 2,928,985 to 2,929,602
_2520
Psyr_2521: Psyr_2521 - conserved hypothetical protein, at 2,929,599 to 2,930,222
_2521
Psyr_2522: Psyr_2522 - conserved hypothetical protein, at 2,930,230 to 2,931,660
_2522
Position (kb)
2928
2929
2930 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2928.170 kb on + strand, within Psyr_2519 at 2928.325 kb on + strand, within Psyr_2519 at 2928.325 kb on + strand, within Psyr_2519 at 2928.325 kb on + strand, within Psyr_2519 at 2928.325 kb on + strand, within Psyr_2519 at 2928.325 kb on + strand, within Psyr_2519 at 2928.326 kb on - strand, within Psyr_2519 at 2928.326 kb on - strand, within Psyr_2519 at 2928.326 kb on - strand, within Psyr_2519 at 2928.416 kb on + strand, within Psyr_2519 at 2928.416 kb on + strand, within Psyr_2519 at 2928.417 kb on - strand, within Psyr_2519 at 2928.417 kb on - strand, within Psyr_2519 at 2928.417 kb on - strand, within Psyr_2519 at 2928.548 kb on + strand, within Psyr_2519 at 2928.549 kb on - strand, within Psyr_2519 at 2928.549 kb on - strand, within Psyr_2519 at 2928.553 kb on - strand, within Psyr_2519 at 2928.553 kb on - strand, within Psyr_2519 at 2928.611 kb on + strand, within Psyr_2519 at 2928.611 kb on + strand, within Psyr_2519 at 2928.612 kb on - strand, within Psyr_2519 at 2928.612 kb on - strand, within Psyr_2519 at 2928.612 kb on - strand, within Psyr_2519 at 2928.701 kb on + strand at 2928.701 kb on + strand at 2928.702 kb on - strand at 2928.702 kb on - strand at 2928.702 kb on - strand at 2928.702 kb on - strand at 2928.892 kb on + strand at 2928.969 kb on - strand at 2929.004 kb on + strand at 2929.005 kb on - strand at 2929.029 kb on + strand at 2929.030 kb on - strand at 2929.336 kb on + strand, within Psyr_2520 at 2929.410 kb on + strand, within Psyr_2520 at 2929.863 kb on + strand, within Psyr_2521 at 2929.863 kb on + strand, within Psyr_2521 at 2929.863 kb on + strand, within Psyr_2521 at 2929.863 kb on + strand, within Psyr_2521 at 2929.864 kb on - strand, within Psyr_2521 at 2929.864 kb on - strand, within Psyr_2521 at 2929.864 kb on - strand, within Psyr_2521 at 2929.864 kb on - strand, within Psyr_2521 at 2930.229 kb on + strand at 2930.229 kb on + strand at 2930.229 kb on + strand at 2930.230 kb on - strand at 2930.494 kb on + strand, within Psyr_2522
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,928,170 + Psyr_2519 0.39 +1.0 2,928,325 + Psyr_2519 0.54 -0.7 2,928,325 + Psyr_2519 0.54 +1.8 2,928,325 + Psyr_2519 0.54 +2.4 2,928,325 + Psyr_2519 0.54 +1.4 2,928,325 + Psyr_2519 0.54 +4.3 2,928,326 - Psyr_2519 0.54 -0.2 2,928,326 - Psyr_2519 0.54 +3.0 2,928,326 - Psyr_2519 0.54 +3.7 2,928,416 + Psyr_2519 0.63 +3.9 2,928,416 + Psyr_2519 0.63 +4.2 2,928,417 - Psyr_2519 0.63 +0.8 2,928,417 - Psyr_2519 0.63 -1.3 2,928,417 - Psyr_2519 0.63 +2.1 2,928,548 + Psyr_2519 0.77 -2.0 2,928,549 - Psyr_2519 0.77 -1.5 2,928,549 - Psyr_2519 0.77 +1.8 2,928,553 - Psyr_2519 0.77 +4.0 2,928,553 - Psyr_2519 0.77 +1.4 2,928,611 + Psyr_2519 0.83 +1.8 2,928,611 + Psyr_2519 0.83 -1.1 2,928,612 - Psyr_2519 0.83 -0.1 2,928,612 - Psyr_2519 0.83 +3.9 2,928,612 - Psyr_2519 0.83 -2.1 2,928,701 + -1.1 2,928,701 + -4.4 2,928,702 - -1.3 2,928,702 - -1.9 2,928,702 - +2.1 2,928,702 - -1.1 2,928,892 + -1.6 2,928,969 - +1.4 2,929,004 + -3.7 2,929,005 - +3.9 2,929,029 + +0.8 2,929,030 - -1.1 2,929,336 + Psyr_2520 0.57 +4.4 2,929,410 + Psyr_2520 0.69 -2.1 2,929,863 + Psyr_2521 0.42 -2.9 2,929,863 + Psyr_2521 0.42 -0.3 2,929,863 + Psyr_2521 0.42 -0.8 2,929,863 + Psyr_2521 0.42 -1.2 2,929,864 - Psyr_2521 0.42 +2.9 2,929,864 - Psyr_2521 0.42 +0.0 2,929,864 - Psyr_2521 0.42 -1.9 2,929,864 - Psyr_2521 0.42 -0.9 2,930,229 + +2.8 2,930,229 + +0.8 2,930,229 + +2.8 2,930,230 - +0.8 2,930,494 + Psyr_2522 0.18 +0.2
Or see this region's nucleotide sequence