Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2516 and Psyr_2517 are separated by 81 nucleotides Psyr_2517 and Psyr_2518 are separated by 293 nucleotides Psyr_2518 and Psyr_2519 are separated by 19 nucleotides
Psyr_2516: Psyr_2516 - ABC transporter, substrate-binding protein, aliphatic sulfonate, at 2,925,448 to 2,926,458
_2516
Psyr_2517: Psyr_2517 - hypothetical protein, at 2,926,540 to 2,926,785
_2517
Psyr_2518: Psyr_2518 - conserved hypothetical protein, at 2,927,079 to 2,927,765
_2518
Psyr_2519: Psyr_2519 - Aldo/keto reductase, at 2,927,785 to 2,928,780
_2519
Position (kb)
2927
2928 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2926.103 kb on + strand, within Psyr_2516 at 2926.103 kb on + strand, within Psyr_2516 at 2926.109 kb on + strand, within Psyr_2516 at 2926.109 kb on + strand, within Psyr_2516 at 2926.109 kb on + strand, within Psyr_2516 at 2926.109 kb on + strand, within Psyr_2516 at 2926.109 kb on + strand, within Psyr_2516 at 2926.109 kb on + strand, within Psyr_2516 at 2926.109 kb on + strand, within Psyr_2516 at 2926.109 kb on + strand, within Psyr_2516 at 2926.110 kb on - strand, within Psyr_2516 at 2926.110 kb on - strand, within Psyr_2516 at 2926.110 kb on - strand, within Psyr_2516 at 2926.110 kb on - strand, within Psyr_2516 at 2926.110 kb on - strand, within Psyr_2516 at 2926.110 kb on - strand, within Psyr_2516 at 2926.110 kb on - strand, within Psyr_2516 at 2926.199 kb on + strand, within Psyr_2516 at 2926.200 kb on - strand, within Psyr_2516 at 2926.307 kb on + strand, within Psyr_2516 at 2926.308 kb on - strand, within Psyr_2516 at 2926.310 kb on + strand, within Psyr_2516 at 2926.310 kb on + strand, within Psyr_2516 at 2926.311 kb on - strand, within Psyr_2516 at 2926.339 kb on + strand, within Psyr_2516 at 2926.340 kb on - strand, within Psyr_2516 at 2926.789 kb on + strand at 2926.812 kb on - strand at 2926.954 kb on + strand at 2927.022 kb on + strand at 2927.084 kb on + strand at 2927.084 kb on + strand at 2927.231 kb on - strand, within Psyr_2518 at 2927.231 kb on - strand, within Psyr_2518 at 2927.403 kb on + strand, within Psyr_2518 at 2927.403 kb on + strand, within Psyr_2518 at 2927.403 kb on + strand, within Psyr_2518 at 2927.409 kb on + strand, within Psyr_2518 at 2927.409 kb on + strand, within Psyr_2518 at 2927.409 kb on + strand, within Psyr_2518 at 2927.409 kb on + strand, within Psyr_2518 at 2927.410 kb on - strand, within Psyr_2518 at 2927.410 kb on - strand, within Psyr_2518 at 2927.410 kb on - strand, within Psyr_2518 at 2927.410 kb on - strand, within Psyr_2518 at 2927.526 kb on + strand, within Psyr_2518 at 2927.526 kb on + strand, within Psyr_2518 at 2927.527 kb on - strand, within Psyr_2518 at 2927.527 kb on - strand, within Psyr_2518 at 2927.544 kb on + strand, within Psyr_2518 at 2927.544 kb on + strand, within Psyr_2518 at 2927.544 kb on + strand, within Psyr_2518 at 2927.544 kb on + strand, within Psyr_2518 at 2927.544 kb on + strand, within Psyr_2518 at 2927.544 kb on + strand, within Psyr_2518 at 2927.544 kb on + strand, within Psyr_2518 at 2927.544 kb on + strand, within Psyr_2518 at 2927.544 kb on + strand, within Psyr_2518 at 2927.707 kb on - strand at 2927.819 kb on + strand at 2927.820 kb on - strand at 2927.925 kb on - strand, within Psyr_2519 at 2928.170 kb on + strand, within Psyr_2519 at 2928.325 kb on + strand, within Psyr_2519 at 2928.325 kb on + strand, within Psyr_2519 at 2928.325 kb on + strand, within Psyr_2519 at 2928.325 kb on + strand, within Psyr_2519 at 2928.325 kb on + strand, within Psyr_2519 at 2928.326 kb on - strand, within Psyr_2519 at 2928.326 kb on - strand, within Psyr_2519 at 2928.326 kb on - strand, within Psyr_2519 at 2928.416 kb on + strand, within Psyr_2519 at 2928.416 kb on + strand, within Psyr_2519 at 2928.417 kb on - strand, within Psyr_2519 at 2928.417 kb on - strand, within Psyr_2519 at 2928.417 kb on - strand, within Psyr_2519 at 2928.548 kb on + strand, within Psyr_2519 at 2928.549 kb on - strand, within Psyr_2519 at 2928.549 kb on - strand, within Psyr_2519 at 2928.553 kb on - strand, within Psyr_2519 at 2928.553 kb on - strand, within Psyr_2519 at 2928.611 kb on + strand, within Psyr_2519 at 2928.611 kb on + strand, within Psyr_2519 at 2928.612 kb on - strand, within Psyr_2519 at 2928.612 kb on - strand, within Psyr_2519 at 2928.612 kb on - strand, within Psyr_2519 at 2928.701 kb on + strand at 2928.701 kb on + strand at 2928.702 kb on - strand at 2928.702 kb on - strand at 2928.702 kb on - strand at 2928.702 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,926,103 + Psyr_2516 0.65 +0.8 2,926,103 + Psyr_2516 0.65 +0.8 2,926,109 + Psyr_2516 0.65 -2.0 2,926,109 + Psyr_2516 0.65 -0.7 2,926,109 + Psyr_2516 0.65 +3.4 2,926,109 + Psyr_2516 0.65 +3.3 2,926,109 + Psyr_2516 0.65 -2.3 2,926,109 + Psyr_2516 0.65 +1.8 2,926,109 + Psyr_2516 0.65 +0.8 2,926,109 + Psyr_2516 0.65 -4.7 2,926,110 - Psyr_2516 0.65 +1.8 2,926,110 - Psyr_2516 0.65 -2.4 2,926,110 - Psyr_2516 0.65 +2.0 2,926,110 - Psyr_2516 0.65 -0.3 2,926,110 - Psyr_2516 0.65 +3.7 2,926,110 - Psyr_2516 0.65 -0.8 2,926,110 - Psyr_2516 0.65 -0.7 2,926,199 + Psyr_2516 0.74 -0.2 2,926,200 - Psyr_2516 0.74 +3.3 2,926,307 + Psyr_2516 0.85 -0.6 2,926,308 - Psyr_2516 0.85 -1.0 2,926,310 + Psyr_2516 0.85 -2.5 2,926,310 + Psyr_2516 0.85 -1.9 2,926,311 - Psyr_2516 0.85 +1.8 2,926,339 + Psyr_2516 0.88 -3.0 2,926,340 - Psyr_2516 0.88 -2.3 2,926,789 + +0.4 2,926,812 - -3.0 2,926,954 + -0.2 2,927,022 + -1.7 2,927,084 + -5.6 2,927,084 + +2.1 2,927,231 - Psyr_2518 0.22 +2.4 2,927,231 - Psyr_2518 0.22 -1.6 2,927,403 + Psyr_2518 0.47 -2.5 2,927,403 + Psyr_2518 0.47 -0.6 2,927,403 + Psyr_2518 0.47 -0.2 2,927,409 + Psyr_2518 0.48 -2.5 2,927,409 + Psyr_2518 0.48 +1.8 2,927,409 + Psyr_2518 0.48 +2.6 2,927,409 + Psyr_2518 0.48 -2.3 2,927,410 - Psyr_2518 0.48 +3.5 2,927,410 - Psyr_2518 0.48 -0.5 2,927,410 - Psyr_2518 0.48 -2.0 2,927,410 - Psyr_2518 0.48 -2.4 2,927,526 + Psyr_2518 0.65 -0.2 2,927,526 + Psyr_2518 0.65 -1.9 2,927,527 - Psyr_2518 0.65 -1.4 2,927,527 - Psyr_2518 0.65 +4.7 2,927,544 + Psyr_2518 0.68 -1.2 2,927,544 + Psyr_2518 0.68 -2.8 2,927,544 + Psyr_2518 0.68 -1.1 2,927,544 + Psyr_2518 0.68 -3.7 2,927,544 + Psyr_2518 0.68 +3.1 2,927,544 + Psyr_2518 0.68 +3.3 2,927,544 + Psyr_2518 0.68 +3.0 2,927,544 + Psyr_2518 0.68 -2.1 2,927,544 + Psyr_2518 0.68 +2.4 2,927,707 - +2.6 2,927,819 + +3.0 2,927,820 - -2.8 2,927,925 - Psyr_2519 0.14 -0.2 2,928,170 + Psyr_2519 0.39 +1.0 2,928,325 + Psyr_2519 0.54 -0.7 2,928,325 + Psyr_2519 0.54 +1.8 2,928,325 + Psyr_2519 0.54 +2.4 2,928,325 + Psyr_2519 0.54 +1.4 2,928,325 + Psyr_2519 0.54 +4.3 2,928,326 - Psyr_2519 0.54 -0.2 2,928,326 - Psyr_2519 0.54 +3.0 2,928,326 - Psyr_2519 0.54 +3.7 2,928,416 + Psyr_2519 0.63 +3.9 2,928,416 + Psyr_2519 0.63 +4.2 2,928,417 - Psyr_2519 0.63 +0.8 2,928,417 - Psyr_2519 0.63 -1.3 2,928,417 - Psyr_2519 0.63 +2.1 2,928,548 + Psyr_2519 0.77 -2.0 2,928,549 - Psyr_2519 0.77 -1.5 2,928,549 - Psyr_2519 0.77 +1.8 2,928,553 - Psyr_2519 0.77 +4.0 2,928,553 - Psyr_2519 0.77 +1.4 2,928,611 + Psyr_2519 0.83 +1.8 2,928,611 + Psyr_2519 0.83 -1.1 2,928,612 - Psyr_2519 0.83 -0.1 2,928,612 - Psyr_2519 0.83 +3.9 2,928,612 - Psyr_2519 0.83 -2.1 2,928,701 + -1.1 2,928,701 + -4.4 2,928,702 - -1.3 2,928,702 - -1.9 2,928,702 - +2.1 2,928,702 - -1.1
Or see this region's nucleotide sequence