Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2513 and Psyr_2514 are separated by 6 nucleotides Psyr_2514 and Psyr_2515 are separated by 3 nucleotides Psyr_2515 and Psyr_2516 overlap by 4 nucleotides
Psyr_2513: Psyr_2513 - extracellular solute-binding protein, family 1, at 2,922,800 to 2,923,879
_2513
Psyr_2514: Psyr_2514 - ABC transporter, at 2,923,886 to 2,924,599
_2514
Psyr_2515: Psyr_2515 - Binding-protein-dependent transport systems inner membrane component, at 2,924,603 to 2,925,451
_2515
Psyr_2516: Psyr_2516 - ABC transporter, substrate-binding protein, aliphatic sulfonate, at 2,925,448 to 2,926,458
_2516
Position (kb)
2923
2924
2925 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2922.887 kb on + strand at 2922.887 kb on + strand at 2922.887 kb on + strand at 2922.887 kb on + strand at 2922.887 kb on + strand at 2922.887 kb on + strand at 2922.887 kb on + strand at 2922.887 kb on + strand at 2922.887 kb on + strand at 2922.887 kb on + strand at 2922.888 kb on - strand at 2922.888 kb on - strand at 2922.888 kb on - strand at 2922.888 kb on - strand at 2922.889 kb on + strand at 2922.889 kb on + strand at 2922.889 kb on + strand at 2922.889 kb on + strand at 2922.889 kb on + strand at 2922.890 kb on - strand at 2922.890 kb on - strand at 2922.890 kb on - strand at 2922.890 kb on - strand at 2922.890 kb on - strand at 2922.890 kb on - strand at 2922.891 kb on + strand at 2922.891 kb on + strand at 2922.891 kb on + strand at 2922.892 kb on - strand at 2922.892 kb on - strand at 2922.945 kb on - strand, within Psyr_2513 at 2922.945 kb on - strand, within Psyr_2513 at 2923.166 kb on + strand, within Psyr_2513 at 2923.167 kb on - strand, within Psyr_2513 at 2923.302 kb on + strand, within Psyr_2513 at 2923.302 kb on + strand, within Psyr_2513 at 2923.303 kb on - strand, within Psyr_2513 at 2923.346 kb on + strand, within Psyr_2513 at 2923.346 kb on + strand, within Psyr_2513 at 2923.346 kb on + strand, within Psyr_2513 at 2923.347 kb on - strand, within Psyr_2513 at 2923.385 kb on + strand, within Psyr_2513 at 2923.424 kb on + strand, within Psyr_2513 at 2923.424 kb on + strand, within Psyr_2513 at 2923.451 kb on + strand, within Psyr_2513 at 2923.452 kb on - strand, within Psyr_2513 at 2923.562 kb on + strand, within Psyr_2513 at 2923.658 kb on + strand, within Psyr_2513 at 2923.659 kb on - strand, within Psyr_2513 at 2923.707 kb on - strand, within Psyr_2513 at 2923.707 kb on - strand, within Psyr_2513 at 2923.773 kb on - strand at 2923.985 kb on + strand, within Psyr_2514 at 2924.043 kb on + strand, within Psyr_2514 at 2924.043 kb on + strand, within Psyr_2514 at 2924.043 kb on + strand, within Psyr_2514 at 2924.044 kb on - strand, within Psyr_2514 at 2924.044 kb on - strand, within Psyr_2514 at 2924.044 kb on - strand, within Psyr_2514 at 2924.044 kb on - strand, within Psyr_2514 at 2924.314 kb on - strand, within Psyr_2514 at 2924.338 kb on + strand, within Psyr_2514 at 2924.414 kb on + strand, within Psyr_2514 at 2924.545 kb on + strand at 2924.576 kb on + strand at 2924.576 kb on + strand at 2924.577 kb on - strand at 2924.577 kb on - strand at 2924.577 kb on - strand at 2924.577 kb on - strand at 2924.601 kb on + strand at 2924.601 kb on + strand at 2924.601 kb on + strand at 2924.601 kb on + strand at 2924.601 kb on + strand at 2924.601 kb on + strand at 2924.602 kb on - strand at 2924.602 kb on - strand at 2924.602 kb on - strand at 2924.602 kb on - strand at 2924.602 kb on - strand at 2924.602 kb on - strand at 2924.602 kb on - strand at 2924.913 kb on + strand, within Psyr_2515 at 2924.913 kb on + strand, within Psyr_2515 at 2924.913 kb on + strand, within Psyr_2515 at 2924.913 kb on + strand, within Psyr_2515 at 2924.913 kb on + strand, within Psyr_2515 at 2924.913 kb on + strand, within Psyr_2515 at 2924.913 kb on + strand, within Psyr_2515 at 2924.914 kb on - strand, within Psyr_2515 at 2924.914 kb on - strand, within Psyr_2515 at 2924.914 kb on - strand, within Psyr_2515 at 2924.914 kb on - strand, within Psyr_2515 at 2924.914 kb on - strand, within Psyr_2515 at 2924.914 kb on - strand, within Psyr_2515 at 2924.914 kb on - strand, within Psyr_2515 at 2924.914 kb on - strand, within Psyr_2515 at 2924.977 kb on - strand, within Psyr_2515 at 2925.097 kb on + strand, within Psyr_2515 at 2925.097 kb on + strand, within Psyr_2515 at 2925.097 kb on + strand, within Psyr_2515 at 2925.098 kb on - strand, within Psyr_2515 at 2925.109 kb on - strand, within Psyr_2515 at 2925.282 kb on + strand, within Psyr_2515
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,922,887 + -4.0 2,922,887 + -2.8 2,922,887 + -2.5 2,922,887 + -0.2 2,922,887 + -2.1 2,922,887 + -1.3 2,922,887 + -0.2 2,922,887 + +1.4 2,922,887 + -3.2 2,922,887 + -1.9 2,922,888 - -3.3 2,922,888 - -4.1 2,922,888 - +3.0 2,922,888 - +0.3 2,922,889 + +1.4 2,922,889 + +3.0 2,922,889 + -2.2 2,922,889 + -2.7 2,922,889 + +0.8 2,922,890 - +0.1 2,922,890 - +0.4 2,922,890 - -1.5 2,922,890 - -0.5 2,922,890 - -0.2 2,922,890 - +1.4 2,922,891 + +1.4 2,922,891 + +0.8 2,922,891 + +2.8 2,922,892 - -0.2 2,922,892 - -1.9 2,922,945 - Psyr_2513 0.13 -1.3 2,922,945 - Psyr_2513 0.13 -0.2 2,923,166 + Psyr_2513 0.34 +3.2 2,923,167 - Psyr_2513 0.34 -2.7 2,923,302 + Psyr_2513 0.46 -2.3 2,923,302 + Psyr_2513 0.46 -0.4 2,923,303 - Psyr_2513 0.47 +2.8 2,923,346 + Psyr_2513 0.51 -0.7 2,923,346 + Psyr_2513 0.51 -0.2 2,923,346 + Psyr_2513 0.51 +3.0 2,923,347 - Psyr_2513 0.51 +1.2 2,923,385 + Psyr_2513 0.54 +3.0 2,923,424 + Psyr_2513 0.58 -1.2 2,923,424 + Psyr_2513 0.58 +3.0 2,923,451 + Psyr_2513 0.60 +0.3 2,923,452 - Psyr_2513 0.60 +2.1 2,923,562 + Psyr_2513 0.71 -2.3 2,923,658 + Psyr_2513 0.79 +3.7 2,923,659 - Psyr_2513 0.80 +3.0 2,923,707 - Psyr_2513 0.84 +1.4 2,923,707 - Psyr_2513 0.84 +3.7 2,923,773 - +2.4 2,923,985 + Psyr_2514 0.14 -2.7 2,924,043 + Psyr_2514 0.22 -1.2 2,924,043 + Psyr_2514 0.22 -1.9 2,924,043 + Psyr_2514 0.22 -2.8 2,924,044 - Psyr_2514 0.22 +1.4 2,924,044 - Psyr_2514 0.22 -3.4 2,924,044 - Psyr_2514 0.22 -0.2 2,924,044 - Psyr_2514 0.22 +3.0 2,924,314 - Psyr_2514 0.60 -0.2 2,924,338 + Psyr_2514 0.63 +1.4 2,924,414 + Psyr_2514 0.74 -2.8 2,924,545 + -0.2 2,924,576 + +1.4 2,924,576 + +3.0 2,924,577 - +0.8 2,924,577 - +1.4 2,924,577 - +2.4 2,924,577 - -0.5 2,924,601 + -2.7 2,924,601 + +1.8 2,924,601 + +3.3 2,924,601 + -1.6 2,924,601 + -2.4 2,924,601 + -0.8 2,924,602 - +3.0 2,924,602 - -0.5 2,924,602 - +1.8 2,924,602 - +1.8 2,924,602 - +0.6 2,924,602 - -1.2 2,924,602 - +3.4 2,924,913 + Psyr_2515 0.37 +0.3 2,924,913 + Psyr_2515 0.37 -0.4 2,924,913 + Psyr_2515 0.37 +1.8 2,924,913 + Psyr_2515 0.37 +0.2 2,924,913 + Psyr_2515 0.37 -0.2 2,924,913 + Psyr_2515 0.37 +2.6 2,924,913 + Psyr_2515 0.37 -4.8 2,924,914 - Psyr_2515 0.37 +2.1 2,924,914 - Psyr_2515 0.37 -0.1 2,924,914 - Psyr_2515 0.37 +1.8 2,924,914 - Psyr_2515 0.37 -1.4 2,924,914 - Psyr_2515 0.37 +2.4 2,924,914 - Psyr_2515 0.37 -0.5 2,924,914 - Psyr_2515 0.37 -1.2 2,924,914 - Psyr_2515 0.37 +1.8 2,924,977 - Psyr_2515 0.44 +2.1 2,925,097 + Psyr_2515 0.58 +0.6 2,925,097 + Psyr_2515 0.58 +1.4 2,925,097 + Psyr_2515 0.58 +3.0 2,925,098 - Psyr_2515 0.58 +2.1 2,925,109 - Psyr_2515 0.60 -1.5 2,925,282 + Psyr_2515 0.80 +0.8
Or see this region's nucleotide sequence