Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2483 and Psyr_2484 overlap by 4 nucleotides Psyr_2484 and Psyr_2485 overlap by 4 nucleotides
Psyr_2483: Psyr_2483 - Acriflavin resistance protein, at 2,880,776 to 2,883,886
_2483
Psyr_2484: Psyr_2484 - Acriflavin resistance protein, at 2,883,883 to 2,886,990
_2484
Psyr_2485: Psyr_2485 - RND efflux system, outer membrane lipoprotein, NodT, at 2,886,987 to 2,888,450
_2485
Position (kb)
2883
2884
2885
2886
2887 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2882.906 kb on + strand, within Psyr_2483 at 2882.907 kb on - strand, within Psyr_2483 at 2883.011 kb on + strand, within Psyr_2483 at 2883.011 kb on + strand, within Psyr_2483 at 2883.011 kb on + strand, within Psyr_2483 at 2883.011 kb on + strand, within Psyr_2483 at 2883.011 kb on + strand, within Psyr_2483 at 2883.011 kb on + strand, within Psyr_2483 at 2883.011 kb on + strand, within Psyr_2483 at 2883.011 kb on + strand, within Psyr_2483 at 2883.012 kb on - strand, within Psyr_2483 at 2883.012 kb on - strand, within Psyr_2483 at 2883.012 kb on - strand, within Psyr_2483 at 2883.012 kb on - strand, within Psyr_2483 at 2883.012 kb on - strand, within Psyr_2483 at 2883.012 kb on - strand, within Psyr_2483 at 2883.383 kb on + strand, within Psyr_2483 at 2883.383 kb on + strand, within Psyr_2483 at 2883.383 kb on + strand, within Psyr_2483 at 2883.383 kb on + strand, within Psyr_2483 at 2883.383 kb on + strand, within Psyr_2483 at 2883.383 kb on + strand, within Psyr_2483 at 2883.383 kb on + strand, within Psyr_2483 at 2883.383 kb on + strand at 2883.383 kb on + strand, within Psyr_2483 at 2883.384 kb on - strand, within Psyr_2483 at 2883.384 kb on - strand, within Psyr_2483 at 2883.384 kb on - strand, within Psyr_2483 at 2883.384 kb on - strand, within Psyr_2483 at 2883.384 kb on - strand, within Psyr_2483 at 2883.384 kb on - strand, within Psyr_2483 at 2883.384 kb on - strand, within Psyr_2483 at 2883.384 kb on - strand, within Psyr_2483 at 2883.384 kb on - strand, within Psyr_2483 at 2883.384 kb on - strand, within Psyr_2483 at 2883.405 kb on - strand, within Psyr_2483 at 2883.413 kb on + strand, within Psyr_2483 at 2883.413 kb on + strand, within Psyr_2483 at 2883.413 kb on + strand, within Psyr_2483 at 2883.413 kb on + strand, within Psyr_2483 at 2883.414 kb on - strand, within Psyr_2483 at 2883.414 kb on - strand, within Psyr_2483 at 2883.414 kb on - strand, within Psyr_2483 at 2883.414 kb on - strand, within Psyr_2483 at 2883.414 kb on - strand, within Psyr_2483 at 2883.414 kb on - strand, within Psyr_2483 at 2883.414 kb on - strand, within Psyr_2483 at 2883.414 kb on - strand, within Psyr_2483 at 2883.414 kb on - strand, within Psyr_2483 at 2883.414 kb on - strand, within Psyr_2483 at 2883.414 kb on - strand, within Psyr_2483 at 2883.623 kb on + strand at 2883.624 kb on - strand at 2883.624 kb on - strand at 2883.806 kb on + strand at 2883.807 kb on - strand at 2883.807 kb on - strand at 2883.807 kb on - strand at 2883.812 kb on + strand at 2883.812 kb on + strand at 2883.813 kb on - strand at 2884.267 kb on + strand, within Psyr_2484 at 2884.268 kb on - strand, within Psyr_2484 at 2884.268 kb on - strand, within Psyr_2484 at 2884.346 kb on - strand, within Psyr_2484 at 2884.520 kb on + strand, within Psyr_2484 at 2884.520 kb on + strand, within Psyr_2484 at 2884.520 kb on + strand, within Psyr_2484 at 2884.521 kb on - strand, within Psyr_2484 at 2884.557 kb on + strand, within Psyr_2484 at 2884.557 kb on + strand, within Psyr_2484 at 2884.558 kb on - strand, within Psyr_2484 at 2884.603 kb on + strand, within Psyr_2484 at 2884.624 kb on + strand, within Psyr_2484 at 2884.706 kb on - strand, within Psyr_2484 at 2884.706 kb on - strand, within Psyr_2484 at 2884.706 kb on - strand, within Psyr_2484 at 2885.277 kb on + strand, within Psyr_2484 at 2885.278 kb on - strand, within Psyr_2484 at 2885.515 kb on + strand, within Psyr_2484 at 2885.515 kb on + strand, within Psyr_2484 at 2885.515 kb on + strand, within Psyr_2484 at 2885.515 kb on + strand, within Psyr_2484 at 2885.516 kb on - strand, within Psyr_2484 at 2885.516 kb on - strand, within Psyr_2484 at 2885.516 kb on - strand, within Psyr_2484 at 2885.875 kb on + strand, within Psyr_2484 at 2885.875 kb on + strand, within Psyr_2484 at 2885.881 kb on + strand, within Psyr_2484 at 2885.881 kb on + strand, within Psyr_2484 at 2885.881 kb on + strand, within Psyr_2484 at 2885.882 kb on - strand, within Psyr_2484 at 2886.085 kb on + strand, within Psyr_2484 at 2886.112 kb on + strand, within Psyr_2484 at 2886.112 kb on + strand, within Psyr_2484 at 2886.112 kb on + strand, within Psyr_2484 at 2886.112 kb on + strand, within Psyr_2484 at 2886.112 kb on + strand, within Psyr_2484 at 2886.112 kb on + strand, within Psyr_2484 at 2886.112 kb on + strand, within Psyr_2484 at 2886.112 kb on + strand, within Psyr_2484 at 2886.113 kb on - strand, within Psyr_2484 at 2886.113 kb on - strand, within Psyr_2484 at 2886.113 kb on - strand, within Psyr_2484 at 2886.113 kb on - strand, within Psyr_2484 at 2886.113 kb on - strand, within Psyr_2484 at 2886.113 kb on - strand, within Psyr_2484 at 2886.160 kb on + strand, within Psyr_2484 at 2886.485 kb on - strand, within Psyr_2484 at 2886.514 kb on + strand, within Psyr_2484 at 2886.515 kb on - strand, within Psyr_2484 at 2886.515 kb on - strand, within Psyr_2484 at 2886.515 kb on - strand, within Psyr_2484 at 2886.515 kb on - strand, within Psyr_2484 at 2886.515 kb on - strand, within Psyr_2484 at 2886.580 kb on + strand, within Psyr_2484 at 2886.580 kb on + strand, within Psyr_2484 at 2886.580 kb on + strand, within Psyr_2484 at 2886.580 kb on + strand, within Psyr_2484 at 2886.581 kb on - strand, within Psyr_2484 at 2886.581 kb on - strand, within Psyr_2484 at 2886.581 kb on - strand, within Psyr_2484 at 2886.581 kb on - strand, within Psyr_2484 at 2886.581 kb on - strand, within Psyr_2484 at 2886.581 kb on - strand, within Psyr_2484 at 2886.581 kb on - strand, within Psyr_2484 at 2886.581 kb on - strand, within Psyr_2484 at 2886.581 kb on - strand, within Psyr_2484 at 2886.581 kb on - strand, within Psyr_2484 at 2886.581 kb on - strand, within Psyr_2484 at 2886.584 kb on + strand, within Psyr_2484 at 2886.584 kb on + strand, within Psyr_2484 at 2886.584 kb on + strand, within Psyr_2484 at 2886.585 kb on - strand, within Psyr_2484 at 2886.585 kb on - strand, within Psyr_2484 at 2886.889 kb on + strand at 2886.890 kb on - strand at 2886.890 kb on - strand at 2886.907 kb on + strand at 2886.913 kb on + strand at 2886.987 kb on - strand at 2887.093 kb on - strand at 2887.198 kb on - strand, within Psyr_2485 at 2887.198 kb on - strand, within Psyr_2485 at 2887.263 kb on + strand, within Psyr_2485 at 2887.416 kb on + strand, within Psyr_2485 at 2887.417 kb on - strand, within Psyr_2485 at 2887.564 kb on - strand, within Psyr_2485 at 2887.564 kb on - strand, within Psyr_2485 at 2887.954 kb on - strand, within Psyr_2485
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,882,906 + Psyr_2483 0.68 +1.8 2,882,907 - Psyr_2483 0.68 +2.4 2,883,011 + Psyr_2483 0.72 -1.0 2,883,011 + Psyr_2483 0.72 -1.2 2,883,011 + Psyr_2483 0.72 -0.2 2,883,011 + Psyr_2483 0.72 -2.2 2,883,011 + Psyr_2483 0.72 -1.7 2,883,011 + Psyr_2483 0.72 -1.5 2,883,011 + Psyr_2483 0.72 -0.9 2,883,011 + Psyr_2483 0.72 +1.8 2,883,012 - Psyr_2483 0.72 +1.4 2,883,012 - Psyr_2483 0.72 +0.8 2,883,012 - Psyr_2483 0.72 +2.8 2,883,012 - Psyr_2483 0.72 -0.2 2,883,012 - Psyr_2483 0.72 +1.4 2,883,012 - Psyr_2483 0.72 +1.8 2,883,383 + Psyr_2483 0.84 +3.1 2,883,383 + Psyr_2483 0.84 +3.2 2,883,383 + Psyr_2483 0.84 -3.4 2,883,383 + Psyr_2483 0.84 -0.9 2,883,383 + Psyr_2483 0.84 -2.2 2,883,383 + Psyr_2483 0.84 -1.5 2,883,383 + Psyr_2483 0.84 -0.2 2,883,383 + -0.2 2,883,383 + Psyr_2483 0.84 -0.6 2,883,384 - Psyr_2483 0.84 -1.5 2,883,384 - Psyr_2483 0.84 +2.1 2,883,384 - Psyr_2483 0.84 -0.7 2,883,384 - Psyr_2483 0.84 +1.8 2,883,384 - Psyr_2483 0.84 +2.1 2,883,384 - Psyr_2483 0.84 +0.8 2,883,384 - Psyr_2483 0.84 -1.0 2,883,384 - Psyr_2483 0.84 +0.8 2,883,384 - Psyr_2483 0.84 -1.5 2,883,384 - Psyr_2483 0.84 +1.8 2,883,405 - Psyr_2483 0.85 +1.8 2,883,413 + Psyr_2483 0.85 +2.1 2,883,413 + Psyr_2483 0.85 +2.4 2,883,413 + Psyr_2483 0.85 +2.8 2,883,413 + Psyr_2483 0.85 +3.6 2,883,414 - Psyr_2483 0.85 -0.3 2,883,414 - Psyr_2483 0.85 +1.4 2,883,414 - Psyr_2483 0.85 -1.6 2,883,414 - Psyr_2483 0.85 +0.8 2,883,414 - Psyr_2483 0.85 -0.2 2,883,414 - Psyr_2483 0.85 +1.8 2,883,414 - Psyr_2483 0.85 -0.2 2,883,414 - Psyr_2483 0.85 -2.3 2,883,414 - Psyr_2483 0.85 +2.1 2,883,414 - Psyr_2483 0.85 -2.7 2,883,414 - Psyr_2483 0.85 -2.8 2,883,623 + +4.0 2,883,624 - +2.2 2,883,624 - -2.8 2,883,806 + +2.8 2,883,807 - -1.1 2,883,807 - -2.0 2,883,807 - -1.2 2,883,812 + +2.1 2,883,812 + +0.8 2,883,813 - -0.2 2,884,267 + Psyr_2484 0.12 +3.6 2,884,268 - Psyr_2484 0.12 +1.4 2,884,268 - Psyr_2484 0.12 +3.2 2,884,346 - Psyr_2484 0.15 +1.8 2,884,520 + Psyr_2484 0.20 +2.8 2,884,520 + Psyr_2484 0.20 +1.8 2,884,520 + Psyr_2484 0.20 -0.4 2,884,521 - Psyr_2484 0.21 -0.2 2,884,557 + Psyr_2484 0.22 -0.0 2,884,557 + Psyr_2484 0.22 -1.3 2,884,558 - Psyr_2484 0.22 +1.8 2,884,603 + Psyr_2484 0.23 +1.8 2,884,624 + Psyr_2484 0.24 +2.1 2,884,706 - Psyr_2484 0.26 +1.8 2,884,706 - Psyr_2484 0.26 +3.0 2,884,706 - Psyr_2484 0.26 +3.2 2,885,277 + Psyr_2484 0.45 +0.6 2,885,278 - Psyr_2484 0.45 -3.0 2,885,515 + Psyr_2484 0.53 +2.1 2,885,515 + Psyr_2484 0.53 +0.4 2,885,515 + Psyr_2484 0.53 +0.6 2,885,515 + Psyr_2484 0.53 +2.4 2,885,516 - Psyr_2484 0.53 +2.1 2,885,516 - Psyr_2484 0.53 -2.3 2,885,516 - Psyr_2484 0.53 +1.4 2,885,875 + Psyr_2484 0.64 +2.4 2,885,875 + Psyr_2484 0.64 +2.8 2,885,881 + Psyr_2484 0.64 +2.8 2,885,881 + Psyr_2484 0.64 -2.9 2,885,881 + Psyr_2484 0.64 -1.8 2,885,882 - Psyr_2484 0.64 +3.5 2,886,085 + Psyr_2484 0.71 -3.4 2,886,112 + Psyr_2484 0.72 +2.1 2,886,112 + Psyr_2484 0.72 +2.1 2,886,112 + Psyr_2484 0.72 -0.2 2,886,112 + Psyr_2484 0.72 +3.2 2,886,112 + Psyr_2484 0.72 -0.2 2,886,112 + Psyr_2484 0.72 +2.6 2,886,112 + Psyr_2484 0.72 +0.8 2,886,112 + Psyr_2484 0.72 +2.1 2,886,113 - Psyr_2484 0.72 +0.3 2,886,113 - Psyr_2484 0.72 -1.7 2,886,113 - Psyr_2484 0.72 -1.2 2,886,113 - Psyr_2484 0.72 +3.5 2,886,113 - Psyr_2484 0.72 +3.0 2,886,113 - Psyr_2484 0.72 +2.1 2,886,160 + Psyr_2484 0.73 -2.5 2,886,485 - Psyr_2484 0.84 -1.0 2,886,514 + Psyr_2484 0.85 +2.8 2,886,515 - Psyr_2484 0.85 -0.3 2,886,515 - Psyr_2484 0.85 +4.2 2,886,515 - Psyr_2484 0.85 +2.4 2,886,515 - Psyr_2484 0.85 -0.2 2,886,515 - Psyr_2484 0.85 -1.5 2,886,580 + Psyr_2484 0.87 -0.2 2,886,580 + Psyr_2484 0.87 -0.2 2,886,580 + Psyr_2484 0.87 -2.4 2,886,580 + Psyr_2484 0.87 +1.8 2,886,581 - Psyr_2484 0.87 -1.6 2,886,581 - Psyr_2484 0.87 +2.9 2,886,581 - Psyr_2484 0.87 +3.9 2,886,581 - Psyr_2484 0.87 +3.2 2,886,581 - Psyr_2484 0.87 +3.9 2,886,581 - Psyr_2484 0.87 +4.2 2,886,581 - Psyr_2484 0.87 +3.2 2,886,581 - Psyr_2484 0.87 +3.1 2,886,581 - Psyr_2484 0.87 +0.0 2,886,581 - Psyr_2484 0.87 +3.6 2,886,581 - Psyr_2484 0.87 +1.1 2,886,584 + Psyr_2484 0.87 -1.1 2,886,584 + Psyr_2484 0.87 -3.5 2,886,584 + Psyr_2484 0.87 -1.7 2,886,585 - Psyr_2484 0.87 +2.1 2,886,585 - Psyr_2484 0.87 +2.1 2,886,889 + -0.2 2,886,890 - +1.4 2,886,890 - +2.1 2,886,907 + -1.5 2,886,913 + -0.6 2,886,987 - -0.2 2,887,093 - +2.6 2,887,198 - Psyr_2485 0.14 +0.6 2,887,198 - Psyr_2485 0.14 +0.8 2,887,263 + Psyr_2485 0.19 +1.8 2,887,416 + Psyr_2485 0.29 +1.8 2,887,417 - Psyr_2485 0.29 -2.2 2,887,564 - Psyr_2485 0.39 -0.5 2,887,564 - Psyr_2485 0.39 +2.1 2,887,954 - Psyr_2485 0.66 +2.9
Or see this region's nucleotide sequence