Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2465 and Psyr_2466 are separated by 70 nucleotides Psyr_2466 and Psyr_2467 are separated by 96 nucleotides Psyr_2467 and Psyr_2468 overlap by 4 nucleotides Psyr_2468 and Psyr_2469 are separated by 14 nucleotides
Psyr_2465: Psyr_2465 - Acyltransferase 3, at 2,857,032 to 2,858,054
_2465
Psyr_2466: Psyr_2466 - HesB/YadR/YfhF:Nitrogen-fixing NifU, C-terminal, at 2,858,125 to 2,858,718
_2466
Psyr_2467: Psyr_2467 - 3-methylcrotonoyl-CoA carboxylase, alpha subunit, at 2,858,815 to 2,860,764
_2467
Psyr_2468: Psyr_2468 - Enoyl-CoA hydratase/isomerase, at 2,860,761 to 2,861,636
_2468
Psyr_2469: Psyr_2469 - Propionyl-CoA carboxylase, at 2,861,651 to 2,863,258
_2469
Position (kb)
2858
2859
2860
2861 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2857.836 kb on + strand, within Psyr_2465 at 2857.845 kb on + strand, within Psyr_2465 at 2857.912 kb on - strand, within Psyr_2465 at 2857.912 kb on - strand, within Psyr_2465 at 2857.912 kb on - strand, within Psyr_2465 at 2857.965 kb on + strand at 2857.965 kb on + strand at 2857.966 kb on - strand at 2858.713 kb on + strand at 2858.713 kb on + strand at 2858.713 kb on + strand at 2858.713 kb on + strand at 2858.714 kb on - strand at 2859.104 kb on + strand, within Psyr_2467 at 2859.105 kb on - strand, within Psyr_2467 at 2859.605 kb on + strand, within Psyr_2467 at 2859.606 kb on - strand at 2859.680 kb on + strand, within Psyr_2467 at 2859.848 kb on + strand, within Psyr_2467 at 2859.848 kb on + strand, within Psyr_2467 at 2859.848 kb on + strand, within Psyr_2467 at 2859.848 kb on + strand, within Psyr_2467 at 2859.849 kb on - strand, within Psyr_2467 at 2859.868 kb on + strand, within Psyr_2467 at 2859.869 kb on - strand, within Psyr_2467 at 2859.951 kb on - strand, within Psyr_2467 at 2860.085 kb on + strand, within Psyr_2467 at 2860.085 kb on + strand, within Psyr_2467 at 2860.148 kb on + strand, within Psyr_2467 at 2860.149 kb on - strand, within Psyr_2467 at 2860.149 kb on - strand, within Psyr_2467 at 2860.574 kb on + strand at 2860.574 kb on + strand at 2860.575 kb on - strand at 2860.575 kb on - strand at 2860.630 kb on - strand at 2861.141 kb on - strand, within Psyr_2468 at 2861.347 kb on + strand, within Psyr_2468 at 2861.347 kb on + strand, within Psyr_2468 at 2861.347 kb on + strand, within Psyr_2468 at 2861.348 kb on - strand, within Psyr_2468 at 2861.392 kb on + strand, within Psyr_2468 at 2861.393 kb on - strand, within Psyr_2468 at 2861.393 kb on - strand, within Psyr_2468 at 2861.497 kb on - strand, within Psyr_2468 at 2861.497 kb on - strand, within Psyr_2468 at 2861.611 kb on - strand at 2861.678 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,857,836 + Psyr_2465 0.79 +0.5 2,857,845 + Psyr_2465 0.79 +2.1 2,857,912 - Psyr_2465 0.86 -4.0 2,857,912 - Psyr_2465 0.86 +2.6 2,857,912 - Psyr_2465 0.86 -0.5 2,857,965 + -1.7 2,857,965 + -0.6 2,857,966 - -1.3 2,858,713 + +0.3 2,858,713 + +2.1 2,858,713 + +2.6 2,858,713 + -5.1 2,858,714 - -0.2 2,859,104 + Psyr_2467 0.15 +1.8 2,859,105 - Psyr_2467 0.15 +0.6 2,859,605 + Psyr_2467 0.41 -2.4 2,859,606 - +1.4 2,859,680 + Psyr_2467 0.44 -2.0 2,859,848 + Psyr_2467 0.53 +0.8 2,859,848 + Psyr_2467 0.53 -0.2 2,859,848 + Psyr_2467 0.53 -0.9 2,859,848 + Psyr_2467 0.53 -1.7 2,859,849 - Psyr_2467 0.53 -0.5 2,859,868 + Psyr_2467 0.54 +1.8 2,859,869 - Psyr_2467 0.54 +2.6 2,859,951 - Psyr_2467 0.58 -1.6 2,860,085 + Psyr_2467 0.65 +0.7 2,860,085 + Psyr_2467 0.65 +2.1 2,860,148 + Psyr_2467 0.68 -1.7 2,860,149 - Psyr_2467 0.68 -0.2 2,860,149 - Psyr_2467 0.68 -1.6 2,860,574 + +1.0 2,860,574 + +3.7 2,860,575 - +3.0 2,860,575 - +0.8 2,860,630 - +2.6 2,861,141 - Psyr_2468 0.43 -2.3 2,861,347 + Psyr_2468 0.67 -3.3 2,861,347 + Psyr_2468 0.67 -1.2 2,861,347 + Psyr_2468 0.67 -0.6 2,861,348 - Psyr_2468 0.67 +0.8 2,861,392 + Psyr_2468 0.72 +2.6 2,861,393 - Psyr_2468 0.72 +0.4 2,861,393 - Psyr_2468 0.72 -0.2 2,861,497 - Psyr_2468 0.84 -0.2 2,861,497 - Psyr_2468 0.84 +1.0 2,861,611 - +1.8 2,861,678 - -2.6
Or see this region's nucleotide sequence