Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2427 and Psyr_RNA41 are separated by 182 nucleotides Psyr_RNA41 and Psyr_2428 are separated by 658 nucleotides Psyr_2428 and Psyr_RNA42 are separated by 578 nucleotides Psyr_RNA42 and Psyr_2429 are separated by 134 nucleotides
Psyr_2427: Psyr_2427 - gamma-glutamylputrescine oxidase, at 2,807,649 to 2,808,932
_2427
Psyr_RNA41: Psyr_RNA41 - tRNA-Val, at 2,809,115 to 2,809,191
_RNA41
Psyr_2428: Psyr_2428 - GCN5-related N-acetyltransferase, at 2,809,850 to 2,810,326
_2428
Psyr_RNA42: Psyr_RNA42 - tRNA-Val, at 2,810,905 to 2,810,981
_RNA42
Psyr_2429: Psyr_2429 - conserved hypothetical protein, at 2,811,116 to 2,812,177
_2429
Position (kb)
2809
2810
2811 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2808.904 kb on + strand at 2808.904 kb on + strand at 2808.905 kb on - strand at 2808.985 kb on + strand at 2809.305 kb on - strand at 2809.325 kb on + strand at 2809.325 kb on + strand at 2809.328 kb on - strand at 2809.364 kb on - strand at 2809.366 kb on + strand at 2809.367 kb on - strand at 2809.379 kb on - strand at 2809.427 kb on + strand at 2809.427 kb on + strand at 2809.428 kb on - strand at 2809.428 kb on - strand at 2809.428 kb on - strand at 2809.429 kb on + strand at 2809.429 kb on + strand at 2809.447 kb on + strand at 2809.482 kb on - strand at 2809.492 kb on + strand at 2809.493 kb on - strand at 2809.526 kb on + strand at 2809.548 kb on - strand at 2809.548 kb on - strand at 2809.548 kb on - strand at 2809.550 kb on + strand at 2809.550 kb on + strand at 2809.550 kb on + strand at 2809.551 kb on - strand at 2809.551 kb on - strand at 2809.551 kb on - strand at 2809.556 kb on + strand at 2809.556 kb on + strand at 2809.597 kb on + strand at 2809.597 kb on + strand at 2809.597 kb on + strand at 2809.598 kb on - strand at 2809.615 kb on - strand at 2809.646 kb on + strand at 2809.647 kb on - strand at 2809.647 kb on - strand at 2809.647 kb on - strand at 2809.661 kb on - strand at 2809.683 kb on + strand at 2809.684 kb on - strand at 2809.684 kb on - strand at 2809.684 kb on - strand at 2809.684 kb on - strand at 2809.689 kb on + strand at 2809.689 kb on + strand at 2809.690 kb on - strand at 2809.690 kb on - strand at 2809.699 kb on - strand at 2809.699 kb on - strand at 2809.703 kb on - strand at 2809.703 kb on - strand at 2809.777 kb on - strand at 2809.919 kb on - strand, within Psyr_2428 at 2810.028 kb on + strand at 2810.057 kb on + strand, within Psyr_2428 at 2810.058 kb on - strand, within Psyr_2428 at 2810.100 kb on - strand, within Psyr_2428 at 2810.131 kb on + strand, within Psyr_2428 at 2810.131 kb on + strand, within Psyr_2428 at 2810.150 kb on + strand, within Psyr_2428 at 2810.154 kb on - strand, within Psyr_2428 at 2810.217 kb on + strand, within Psyr_2428 at 2810.217 kb on + strand, within Psyr_2428 at 2810.218 kb on - strand, within Psyr_2428 at 2810.373 kb on + strand at 2810.411 kb on + strand at 2810.416 kb on + strand at 2810.416 kb on + strand at 2810.428 kb on - strand at 2810.428 kb on - strand at 2810.654 kb on + strand at 2810.654 kb on + strand at 2810.654 kb on + strand at 2810.654 kb on + strand at 2810.655 kb on - strand at 2810.655 kb on - strand at 2810.655 kb on - strand at 2810.667 kb on - strand at 2810.667 kb on - strand at 2810.683 kb on + strand at 2810.707 kb on - strand at 2810.712 kb on + strand at 2810.712 kb on + strand at 2810.713 kb on - strand at 2810.713 kb on - strand at 2810.721 kb on + strand at 2810.722 kb on - strand at 2810.722 kb on - strand at 2810.722 kb on - strand at 2810.725 kb on + strand at 2810.725 kb on + strand at 2810.726 kb on - strand at 2810.726 kb on - strand at 2811.060 kb on + strand at 2811.060 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,808,904 + -0.4 2,808,904 + +0.8 2,808,905 - +4.0 2,808,985 + +2.8 2,809,305 - +2.6 2,809,325 + -4.0 2,809,325 + -3.3 2,809,328 - -2.7 2,809,364 - +1.8 2,809,366 + -0.2 2,809,367 - +2.1 2,809,379 - +2.8 2,809,427 + -2.0 2,809,427 + +0.8 2,809,428 - -1.4 2,809,428 - -1.0 2,809,428 - +1.4 2,809,429 + +2.4 2,809,429 + +3.4 2,809,447 + +0.8 2,809,482 - -0.4 2,809,492 + +3.0 2,809,493 - +1.8 2,809,526 + -0.4 2,809,548 - +1.4 2,809,548 - -1.4 2,809,548 - +2.1 2,809,550 + +0.5 2,809,550 + +3.0 2,809,550 + +3.3 2,809,551 - -2.0 2,809,551 - -1.9 2,809,551 - +2.1 2,809,556 + -0.5 2,809,556 + -2.1 2,809,597 + -1.3 2,809,597 + +0.8 2,809,597 + -2.0 2,809,598 - -0.5 2,809,615 - +3.0 2,809,646 + -2.4 2,809,647 - -0.9 2,809,647 - -0.2 2,809,647 - +3.7 2,809,661 - -1.3 2,809,683 + -1.4 2,809,684 - +3.0 2,809,684 - +4.0 2,809,684 - -1.0 2,809,684 - -1.3 2,809,689 + +1.8 2,809,689 + +2.8 2,809,690 - -1.8 2,809,690 - -0.9 2,809,699 - +3.6 2,809,699 - +0.8 2,809,703 - -0.2 2,809,703 - +0.0 2,809,777 - +0.8 2,809,919 - Psyr_2428 0.14 -2.0 2,810,028 + -3.3 2,810,057 + Psyr_2428 0.43 -4.0 2,810,058 - Psyr_2428 0.44 +3.3 2,810,100 - Psyr_2428 0.52 +0.5 2,810,131 + Psyr_2428 0.59 -0.5 2,810,131 + Psyr_2428 0.59 +1.0 2,810,150 + Psyr_2428 0.63 -1.8 2,810,154 - Psyr_2428 0.64 -2.0 2,810,217 + Psyr_2428 0.77 +2.4 2,810,217 + Psyr_2428 0.77 -1.9 2,810,218 - Psyr_2428 0.77 -1.7 2,810,373 + -3.9 2,810,411 + +2.8 2,810,416 + -1.0 2,810,416 + -2.0 2,810,428 - -0.7 2,810,428 - +2.1 2,810,654 + +1.8 2,810,654 + -2.3 2,810,654 + -1.6 2,810,654 + +3.0 2,810,655 - +3.4 2,810,655 - +1.8 2,810,655 - -0.8 2,810,667 - -1.3 2,810,667 - -0.3 2,810,683 + +2.8 2,810,707 - -0.0 2,810,712 + -0.2 2,810,712 + -2.1 2,810,713 - +2.4 2,810,713 - +1.8 2,810,721 + -2.9 2,810,722 - -1.0 2,810,722 - +2.6 2,810,722 - +3.3 2,810,725 + -1.8 2,810,725 + -1.4 2,810,726 - +4.1 2,810,726 - +1.8 2,811,060 + -0.7 2,811,060 + -1.1
Or see this region's nucleotide sequence