Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2399 and Psyr_2400 are separated by 6 nucleotides Psyr_2400 and Psyr_2401 overlap by 16 nucleotides Psyr_2401 and Psyr_2402 are separated by 290 nucleotides
Psyr_2399: Psyr_2399 - Binding-protein-dependent transport systems inner membrane component, at 2,781,346 to 2,782,161
_2399
Psyr_2400: Psyr_2400 - Binding-protein-dependent transport systems inner membrane component, at 2,782,168 to 2,783,124
_2400
Psyr_2401: Psyr_2401 - extracellular solute-binding protein, family 1, at 2,783,109 to 2,784,134
_2401
Psyr_2402: Psyr_2402 - 3,4-dihydroxy-2-butanone 4-phosphate synthase / GTP cyclohydrolase II, at 2,784,425 to 2,785,534
_2402
Position (kb)
2783
2784
2785 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2782.112 kb on + strand at 2782.112 kb on + strand at 2782.223 kb on + strand at 2782.223 kb on + strand at 2782.223 kb on + strand at 2782.223 kb on + strand at 2782.224 kb on - strand at 2782.224 kb on - strand at 2782.364 kb on + strand, within Psyr_2400 at 2782.364 kb on + strand, within Psyr_2400 at 2782.364 kb on + strand, within Psyr_2400 at 2782.364 kb on + strand, within Psyr_2400 at 2782.364 kb on + strand, within Psyr_2400 at 2782.364 kb on + strand, within Psyr_2400 at 2782.364 kb on + strand, within Psyr_2400 at 2782.364 kb on + strand, within Psyr_2400 at 2782.365 kb on - strand, within Psyr_2400 at 2782.365 kb on - strand, within Psyr_2400 at 2782.365 kb on - strand, within Psyr_2400 at 2782.365 kb on - strand, within Psyr_2400 at 2782.553 kb on + strand, within Psyr_2400 at 2782.553 kb on + strand, within Psyr_2400 at 2782.553 kb on + strand, within Psyr_2400 at 2782.553 kb on + strand, within Psyr_2400 at 2782.553 kb on + strand, within Psyr_2400 at 2782.553 kb on + strand, within Psyr_2400 at 2782.553 kb on + strand, within Psyr_2400 at 2782.553 kb on + strand, within Psyr_2400 at 2782.553 kb on + strand, within Psyr_2400 at 2782.554 kb on - strand, within Psyr_2400 at 2782.554 kb on - strand, within Psyr_2400 at 2782.554 kb on - strand, within Psyr_2400 at 2782.554 kb on - strand, within Psyr_2400 at 2782.554 kb on - strand, within Psyr_2400 at 2782.676 kb on + strand, within Psyr_2400 at 2782.827 kb on + strand, within Psyr_2400 at 2782.827 kb on + strand, within Psyr_2400 at 2782.828 kb on - strand, within Psyr_2400 at 2782.874 kb on + strand, within Psyr_2400 at 2782.874 kb on + strand, within Psyr_2400 at 2782.874 kb on + strand, within Psyr_2400 at 2782.875 kb on - strand, within Psyr_2400 at 2782.875 kb on - strand, within Psyr_2400 at 2782.875 kb on - strand, within Psyr_2400 at 2782.918 kb on + strand, within Psyr_2400 at 2783.110 kb on + strand at 2783.110 kb on + strand at 2783.110 kb on + strand at 2783.110 kb on + strand at 2783.111 kb on - strand at 2783.168 kb on - strand at 2783.217 kb on - strand, within Psyr_2401 at 2783.269 kb on + strand, within Psyr_2401 at 2783.269 kb on + strand, within Psyr_2401 at 2783.441 kb on - strand, within Psyr_2401 at 2783.650 kb on + strand, within Psyr_2401 at 2783.651 kb on - strand, within Psyr_2401 at 2783.726 kb on + strand, within Psyr_2401 at 2783.727 kb on - strand, within Psyr_2401 at 2783.737 kb on + strand, within Psyr_2401 at 2783.737 kb on + strand, within Psyr_2401 at 2784.196 kb on + strand at 2784.197 kb on - strand at 2784.197 kb on - strand at 2784.222 kb on + strand at 2784.222 kb on + strand at 2784.223 kb on - strand at 2784.239 kb on + strand at 2784.239 kb on + strand at 2784.240 kb on - strand at 2784.240 kb on - strand at 2784.240 kb on - strand at 2784.442 kb on + strand at 2784.484 kb on - strand at 2784.484 kb on - strand at 2784.484 kb on - strand at 2784.564 kb on + strand, within Psyr_2402 at 2784.564 kb on + strand, within Psyr_2402 at 2784.565 kb on - strand, within Psyr_2402 at 2784.589 kb on - strand, within Psyr_2402 at 2784.589 kb on - strand, within Psyr_2402 at 2784.665 kb on + strand, within Psyr_2402 at 2784.736 kb on - strand, within Psyr_2402 at 2784.858 kb on + strand, within Psyr_2402 at 2784.858 kb on + strand, within Psyr_2402
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,782,112 + -1.8 2,782,112 + +2.7 2,782,223 + -0.5 2,782,223 + +3.3 2,782,223 + -2.4 2,782,223 + +1.9 2,782,224 - +2.7 2,782,224 - -1.6 2,782,364 + Psyr_2400 0.20 -1.1 2,782,364 + Psyr_2400 0.20 -2.6 2,782,364 + Psyr_2400 0.20 -2.1 2,782,364 + Psyr_2400 0.20 +2.2 2,782,364 + Psyr_2400 0.20 -0.2 2,782,364 + Psyr_2400 0.20 +3.0 2,782,364 + Psyr_2400 0.20 +0.9 2,782,364 + Psyr_2400 0.20 -0.1 2,782,365 - Psyr_2400 0.21 +1.9 2,782,365 - Psyr_2400 0.21 +0.3 2,782,365 - Psyr_2400 0.21 +2.7 2,782,365 - Psyr_2400 0.21 -0.1 2,782,553 + Psyr_2400 0.40 +0.3 2,782,553 + Psyr_2400 0.40 +3.3 2,782,553 + Psyr_2400 0.40 +0.9 2,782,553 + Psyr_2400 0.40 -0.9 2,782,553 + Psyr_2400 0.40 +3.0 2,782,553 + Psyr_2400 0.40 -1.7 2,782,553 + Psyr_2400 0.40 +1.9 2,782,553 + Psyr_2400 0.40 +1.5 2,782,553 + Psyr_2400 0.40 -2.1 2,782,554 - Psyr_2400 0.40 -3.5 2,782,554 - Psyr_2400 0.40 +0.3 2,782,554 - Psyr_2400 0.40 -0.7 2,782,554 - Psyr_2400 0.40 -2.9 2,782,554 - Psyr_2400 0.40 +2.7 2,782,676 + Psyr_2400 0.53 +2.9 2,782,827 + Psyr_2400 0.69 +2.7 2,782,827 + Psyr_2400 0.69 +0.9 2,782,828 - Psyr_2400 0.69 +3.0 2,782,874 + Psyr_2400 0.74 +1.5 2,782,874 + Psyr_2400 0.74 +3.0 2,782,874 + Psyr_2400 0.74 +1.5 2,782,875 - Psyr_2400 0.74 -0.8 2,782,875 - Psyr_2400 0.74 -1.1 2,782,875 - Psyr_2400 0.74 +2.9 2,782,918 + Psyr_2400 0.78 -0.7 2,783,110 + +2.9 2,783,110 + +3.8 2,783,110 + +3.0 2,783,110 + +3.2 2,783,111 - +3.7 2,783,168 - +2.5 2,783,217 - Psyr_2401 0.11 +4.2 2,783,269 + Psyr_2401 0.16 +2.2 2,783,269 + Psyr_2401 0.16 +1.1 2,783,441 - Psyr_2401 0.32 +1.5 2,783,650 + Psyr_2401 0.53 +3.0 2,783,651 - Psyr_2401 0.53 +3.2 2,783,726 + Psyr_2401 0.60 +2.5 2,783,727 - Psyr_2401 0.60 +2.2 2,783,737 + Psyr_2401 0.61 +2.5 2,783,737 + Psyr_2401 0.61 +2.2 2,784,196 + -1.5 2,784,197 - -3.2 2,784,197 - +1.9 2,784,222 + +2.2 2,784,222 + -2.2 2,784,223 - +3.3 2,784,239 + +3.6 2,784,239 + -2.4 2,784,240 - -1.5 2,784,240 - +1.9 2,784,240 - -1.9 2,784,442 + -0.6 2,784,484 - +1.5 2,784,484 - -0.1 2,784,484 - -0.1 2,784,564 + Psyr_2402 0.13 +2.9 2,784,564 + Psyr_2402 0.13 +3.3 2,784,565 - Psyr_2402 0.13 +2.2 2,784,589 - Psyr_2402 0.15 -0.5 2,784,589 - Psyr_2402 0.15 +0.1 2,784,665 + Psyr_2402 0.22 -1.9 2,784,736 - Psyr_2402 0.28 -0.2 2,784,858 + Psyr_2402 0.39 +0.9 2,784,858 + Psyr_2402 0.39 +3.3
Or see this region's nucleotide sequence