Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2391 and Psyr_2392 overlap by 20 nucleotides Psyr_2392 and Psyr_2393 are separated by 141 nucleotides Psyr_2393 and Psyr_2394 are separated by 233 nucleotides
Psyr_2391: Psyr_2391 - Protein of unknown function DUF182, at 2,773,157 to 2,774,125
_2391
Psyr_2392: Psyr_2392 - conserved hypothetical protein, at 2,774,106 to 2,774,729
_2392
Psyr_2393: Psyr_2393 - Flavin reductase-like protein, at 2,774,871 to 2,775,455
_2393
Psyr_2394: Psyr_2394 - succinate semialdehyde dehydrogenase, at 2,775,689 to 2,777,080
_2394
Position (kb)
2774
2775
2776 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2773.919 kb on + strand, within Psyr_2391 at 2773.919 kb on + strand, within Psyr_2391 at 2773.919 kb on + strand, within Psyr_2391 at 2773.919 kb on + strand, within Psyr_2391 at 2773.919 kb on + strand, within Psyr_2391 at 2773.919 kb on + strand, within Psyr_2391 at 2773.920 kb on - strand, within Psyr_2391 at 2773.920 kb on - strand, within Psyr_2391 at 2773.920 kb on - strand, within Psyr_2391 at 2773.920 kb on - strand, within Psyr_2391 at 2773.920 kb on - strand, within Psyr_2391 at 2773.920 kb on - strand, within Psyr_2391 at 2773.923 kb on + strand, within Psyr_2391 at 2773.923 kb on + strand, within Psyr_2391 at 2773.923 kb on + strand, within Psyr_2391 at 2773.923 kb on + strand, within Psyr_2391 at 2773.924 kb on - strand, within Psyr_2391 at 2773.924 kb on - strand, within Psyr_2391 at 2773.924 kb on - strand, within Psyr_2391 at 2773.924 kb on - strand, within Psyr_2391 at 2773.924 kb on - strand, within Psyr_2391 at 2773.924 kb on - strand, within Psyr_2391 at 2773.924 kb on - strand, within Psyr_2391 at 2773.924 kb on - strand, within Psyr_2391 at 2774.123 kb on + strand at 2774.123 kb on + strand at 2774.123 kb on + strand at 2774.123 kb on + strand at 2774.124 kb on - strand at 2774.197 kb on + strand, within Psyr_2392 at 2774.197 kb on + strand, within Psyr_2392 at 2774.197 kb on + strand, within Psyr_2392 at 2774.198 kb on - strand, within Psyr_2392 at 2774.198 kb on - strand, within Psyr_2392 at 2774.198 kb on - strand, within Psyr_2392 at 2774.198 kb on - strand, within Psyr_2392 at 2774.198 kb on - strand, within Psyr_2392 at 2774.202 kb on + strand, within Psyr_2392 at 2774.202 kb on + strand, within Psyr_2392 at 2774.203 kb on - strand, within Psyr_2392 at 2774.203 kb on - strand, within Psyr_2392 at 2774.203 kb on - strand, within Psyr_2392 at 2774.203 kb on - strand, within Psyr_2392 at 2774.388 kb on + strand, within Psyr_2392 at 2774.559 kb on + strand, within Psyr_2392 at 2774.560 kb on - strand, within Psyr_2392 at 2774.560 kb on - strand, within Psyr_2392 at 2774.718 kb on + strand at 2774.718 kb on + strand at 2774.718 kb on + strand at 2774.719 kb on - strand at 2774.719 kb on - strand at 2774.719 kb on - strand at 2774.719 kb on - strand at 2774.740 kb on + strand at 2774.883 kb on - strand at 2774.883 kb on - strand at 2775.022 kb on + strand, within Psyr_2393 at 2775.023 kb on - strand, within Psyr_2393 at 2775.282 kb on + strand, within Psyr_2393 at 2775.283 kb on - strand, within Psyr_2393 at 2775.283 kb on - strand, within Psyr_2393 at 2775.390 kb on + strand, within Psyr_2393 at 2775.391 kb on - strand, within Psyr_2393 at 2775.411 kb on + strand at 2775.411 kb on + strand at 2775.412 kb on - strand at 2775.412 kb on - strand at 2775.412 kb on - strand at 2775.413 kb on + strand at 2775.413 kb on + strand at 2775.414 kb on - strand at 2775.603 kb on + strand at 2775.604 kb on - strand at 2775.675 kb on + strand at 2775.675 kb on + strand at 2775.675 kb on + strand at 2775.676 kb on - strand at 2775.676 kb on - strand at 2775.886 kb on - strand, within Psyr_2394 at 2776.041 kb on + strand, within Psyr_2394 at 2776.041 kb on + strand, within Psyr_2394 at 2776.042 kb on - strand, within Psyr_2394 at 2776.042 kb on - strand, within Psyr_2394 at 2776.110 kb on - strand, within Psyr_2394 at 2776.162 kb on - strand, within Psyr_2394 at 2776.162 kb on - strand, within Psyr_2394
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,773,919 + Psyr_2391 0.79 -2.6 2,773,919 + Psyr_2391 0.79 -2.2 2,773,919 + Psyr_2391 0.79 -0.1 2,773,919 + Psyr_2391 0.79 +2.9 2,773,919 + Psyr_2391 0.79 +1.9 2,773,919 + Psyr_2391 0.79 +2.2 2,773,920 - Psyr_2391 0.79 -0.3 2,773,920 - Psyr_2391 0.79 +0.6 2,773,920 - Psyr_2391 0.79 -1.3 2,773,920 - Psyr_2391 0.79 +1.5 2,773,920 - Psyr_2391 0.79 +1.5 2,773,920 - Psyr_2391 0.79 -2.1 2,773,923 + Psyr_2391 0.79 +1.5 2,773,923 + Psyr_2391 0.79 +3.6 2,773,923 + Psyr_2391 0.79 -1.3 2,773,923 + Psyr_2391 0.79 -1.2 2,773,924 - Psyr_2391 0.79 -0.1 2,773,924 - Psyr_2391 0.79 -0.1 2,773,924 - Psyr_2391 0.79 +0.9 2,773,924 - Psyr_2391 0.79 -5.2 2,773,924 - Psyr_2391 0.79 -0.1 2,773,924 - Psyr_2391 0.79 +1.5 2,773,924 - Psyr_2391 0.79 +1.5 2,773,924 - Psyr_2391 0.79 -2.3 2,774,123 + -2.0 2,774,123 + +2.5 2,774,123 + +1.5 2,774,123 + +3.7 2,774,124 - +3.0 2,774,197 + Psyr_2392 0.15 -0.1 2,774,197 + Psyr_2392 0.15 +2.7 2,774,197 + Psyr_2392 0.15 -4.0 2,774,198 - Psyr_2392 0.15 +2.2 2,774,198 - Psyr_2392 0.15 -2.6 2,774,198 - Psyr_2392 0.15 -0.8 2,774,198 - Psyr_2392 0.15 +0.9 2,774,198 - Psyr_2392 0.15 -0.9 2,774,202 + Psyr_2392 0.15 +4.0 2,774,202 + Psyr_2392 0.15 +2.5 2,774,203 - Psyr_2392 0.16 -2.1 2,774,203 - Psyr_2392 0.16 +2.7 2,774,203 - Psyr_2392 0.16 +1.5 2,774,203 - Psyr_2392 0.16 -1.0 2,774,388 + Psyr_2392 0.45 +2.9 2,774,559 + Psyr_2392 0.73 +1.9 2,774,560 - Psyr_2392 0.73 +3.8 2,774,560 - Psyr_2392 0.73 +3.0 2,774,718 + +2.2 2,774,718 + +1.9 2,774,718 + -2.0 2,774,719 - +4.1 2,774,719 - +2.9 2,774,719 - -0.7 2,774,719 - -0.7 2,774,740 + +1.1 2,774,883 - -0.4 2,774,883 - +2.5 2,775,022 + Psyr_2393 0.26 -2.0 2,775,023 - Psyr_2393 0.26 -2.8 2,775,282 + Psyr_2393 0.70 -1.1 2,775,283 - Psyr_2393 0.70 +3.6 2,775,283 - Psyr_2393 0.70 -1.7 2,775,390 + Psyr_2393 0.89 -1.1 2,775,391 - Psyr_2393 0.89 -3.4 2,775,411 + -3.1 2,775,411 + -1.3 2,775,412 - -1.5 2,775,412 - -1.3 2,775,412 - -1.2 2,775,413 + -1.1 2,775,413 + -1.8 2,775,414 - -0.3 2,775,603 + -0.5 2,775,604 - +4.1 2,775,675 + -4.7 2,775,675 + -2.5 2,775,675 + -4.2 2,775,676 - +1.5 2,775,676 - -0.0 2,775,886 - Psyr_2394 0.14 +4.3 2,776,041 + Psyr_2394 0.25 +0.3 2,776,041 + Psyr_2394 0.25 -2.2 2,776,042 - Psyr_2394 0.25 -1.7 2,776,042 - Psyr_2394 0.25 -0.3 2,776,110 - Psyr_2394 0.30 +2.7 2,776,162 - Psyr_2394 0.34 -0.5 2,776,162 - Psyr_2394 0.34 +1.9
Or see this region's nucleotide sequence