Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2349 and Psyr_2350 are separated by 411 nucleotides Psyr_2350 and Psyr_2351 are separated by 78 nucleotides Psyr_2351 and Psyr_2352 are separated by 289 nucleotides
Psyr_2349: Psyr_2349 - hypothetical protein, at 2,725,826 to 2,726,128
_2349
Psyr_2350: Psyr_2350 - Protein of unknown function UPF0025, at 2,726,540 to 2,726,998
_2350
Psyr_2351: Psyr_2351 - tRNA synthetase, class-II (G, H, P and S), at 2,727,077 to 2,727,658
_2351
Psyr_2352: Psyr_2352 - GCN5-related N-acetyltransferase, at 2,727,948 to 2,728,475
_2352
Position (kb)
2726
2727 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2725.599 kb on - strand at 2725.599 kb on - strand at 2725.605 kb on - strand at 2725.795 kb on - strand at 2725.805 kb on + strand at 2725.805 kb on + strand at 2725.805 kb on + strand at 2725.805 kb on + strand at 2725.805 kb on + strand at 2725.805 kb on + strand at 2725.805 kb on + strand at 2725.806 kb on - strand at 2725.806 kb on - strand at 2725.806 kb on - strand at 2725.842 kb on + strand at 2725.842 kb on + strand at 2725.842 kb on + strand at 2725.843 kb on - strand at 2725.910 kb on + strand, within Psyr_2349 at 2726.126 kb on + strand at 2726.126 kb on + strand at 2726.168 kb on - strand at 2726.168 kb on - strand at 2726.173 kb on + strand at 2726.173 kb on + strand at 2726.174 kb on - strand at 2726.180 kb on + strand at 2726.180 kb on + strand at 2726.180 kb on + strand at 2726.180 kb on + strand at 2726.181 kb on - strand at 2726.181 kb on - strand at 2726.181 kb on - strand at 2726.181 kb on - strand at 2726.181 kb on - strand at 2726.181 kb on - strand at 2726.210 kb on + strand at 2726.211 kb on - strand at 2726.236 kb on - strand at 2726.242 kb on + strand at 2726.268 kb on - strand at 2726.288 kb on + strand at 2726.426 kb on + strand at 2726.426 kb on + strand at 2726.426 kb on + strand at 2726.431 kb on - strand at 2726.451 kb on + strand at 2726.451 kb on + strand at 2726.451 kb on + strand at 2726.451 kb on + strand at 2726.451 kb on + strand at 2726.451 kb on + strand at 2726.451 kb on + strand at 2726.452 kb on - strand at 2726.452 kb on - strand at 2726.452 kb on - strand at 2726.452 kb on - strand at 2726.452 kb on - strand at 2726.452 kb on - strand at 2726.452 kb on - strand at 2726.519 kb on + strand at 2726.594 kb on + strand, within Psyr_2350 at 2726.652 kb on + strand, within Psyr_2350 at 2726.652 kb on + strand, within Psyr_2350 at 2726.652 kb on + strand, within Psyr_2350 at 2726.653 kb on - strand, within Psyr_2350 at 2726.653 kb on - strand, within Psyr_2350 at 2727.026 kb on - strand at 2727.026 kb on - strand at 2727.039 kb on - strand at 2727.055 kb on - strand at 2727.064 kb on + strand at 2727.101 kb on + strand at 2727.219 kb on + strand, within Psyr_2351 at 2727.219 kb on + strand, within Psyr_2351 at 2727.220 kb on - strand, within Psyr_2351 at 2727.222 kb on + strand, within Psyr_2351 at 2727.222 kb on + strand, within Psyr_2351 at 2727.222 kb on + strand, within Psyr_2351 at 2727.222 kb on + strand, within Psyr_2351 at 2727.222 kb on + strand, within Psyr_2351 at 2727.223 kb on - strand, within Psyr_2351 at 2727.223 kb on - strand, within Psyr_2351 at 2727.259 kb on - strand, within Psyr_2351 at 2727.631 kb on + strand at 2727.631 kb on + strand at 2727.632 kb on - strand at 2727.632 kb on - strand at 2727.632 kb on - strand at 2727.702 kb on + strand at 2727.743 kb on + strand at 2727.926 kb on + strand at 2727.926 kb on + strand at 2727.949 kb on + strand at 2727.950 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,725,599 - +0.9 2,725,599 - -1.7 2,725,605 - -3.0 2,725,795 - -2.7 2,725,805 + +2.9 2,725,805 + +0.2 2,725,805 + +0.3 2,725,805 + -0.5 2,725,805 + -0.1 2,725,805 + +3.2 2,725,805 + -1.1 2,725,806 - -2.1 2,725,806 - -1.7 2,725,806 - -2.5 2,725,842 + -1.1 2,725,842 + -0.3 2,725,842 + +1.5 2,725,843 - -2.1 2,725,910 + Psyr_2349 0.28 +3.2 2,726,126 + -3.0 2,726,126 + -1.5 2,726,168 - -0.1 2,726,168 - -2.8 2,726,173 + +1.5 2,726,173 + +0.7 2,726,174 - -1.1 2,726,180 + -0.6 2,726,180 + +1.5 2,726,180 + -0.1 2,726,180 + -0.4 2,726,181 - -0.1 2,726,181 - +0.9 2,726,181 - -3.3 2,726,181 - -2.9 2,726,181 - -0.1 2,726,181 - -0.1 2,726,210 + -0.5 2,726,211 - +2.2 2,726,236 - -3.2 2,726,242 + +1.9 2,726,268 - -1.1 2,726,288 + +2.7 2,726,426 + +1.9 2,726,426 + -1.8 2,726,426 + +2.7 2,726,431 - -1.1 2,726,451 + -0.1 2,726,451 + +2.7 2,726,451 + +2.2 2,726,451 + +2.5 2,726,451 + +2.5 2,726,451 + -0.0 2,726,451 + -1.5 2,726,452 - +2.2 2,726,452 - -1.0 2,726,452 - +3.0 2,726,452 - +2.9 2,726,452 - +0.9 2,726,452 - +3.3 2,726,452 - +0.7 2,726,519 + -0.1 2,726,594 + Psyr_2350 0.12 +2.2 2,726,652 + Psyr_2350 0.24 +2.5 2,726,652 + Psyr_2350 0.24 +3.8 2,726,652 + Psyr_2350 0.24 +3.0 2,726,653 - Psyr_2350 0.25 -0.5 2,726,653 - Psyr_2350 0.25 -0.3 2,727,026 - +3.6 2,727,026 - +2.9 2,727,039 - -1.6 2,727,055 - -0.4 2,727,064 + +2.2 2,727,101 + +3.2 2,727,219 + Psyr_2351 0.24 -2.0 2,727,219 + Psyr_2351 0.24 -1.9 2,727,220 - Psyr_2351 0.25 +0.2 2,727,222 + Psyr_2351 0.25 +0.9 2,727,222 + Psyr_2351 0.25 +1.9 2,727,222 + Psyr_2351 0.25 +1.5 2,727,222 + Psyr_2351 0.25 -0.1 2,727,222 + Psyr_2351 0.25 -1.1 2,727,223 - Psyr_2351 0.25 -1.1 2,727,223 - Psyr_2351 0.25 -2.2 2,727,259 - Psyr_2351 0.31 -0.4 2,727,631 + -0.6 2,727,631 + -1.1 2,727,632 - -2.3 2,727,632 - -1.9 2,727,632 - +0.9 2,727,702 + -0.9 2,727,743 + +0.5 2,727,926 + -2.0 2,727,926 + +0.5 2,727,949 + -0.9 2,727,950 - -2.1
Or see this region's nucleotide sequence