Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2346 and Psyr_2347 are separated by 271 nucleotides Psyr_2347 and Psyr_2348 are separated by 140 nucleotides
Psyr_2346: Psyr_2346 - conserved hypothetical protein, at 2,722,690 to 2,723,565
_2346
Psyr_2347: Psyr_2347 - hypothetical protein, at 2,723,837 to 2,724,484
_2347
Psyr_2348: Psyr_2348 - hypothetical protein, at 2,724,625 to 2,725,374
_2348
Position (kb)
2723
2724
2725 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2722.854 kb on + strand, within Psyr_2346 at 2722.854 kb on + strand, within Psyr_2346 at 2722.923 kb on - strand, within Psyr_2346 at 2722.923 kb on - strand, within Psyr_2346 at 2722.990 kb on - strand, within Psyr_2346 at 2723.070 kb on + strand, within Psyr_2346 at 2723.070 kb on + strand, within Psyr_2346 at 2723.075 kb on - strand, within Psyr_2346 at 2723.121 kb on + strand, within Psyr_2346 at 2723.121 kb on + strand, within Psyr_2346 at 2723.221 kb on + strand, within Psyr_2346 at 2723.222 kb on - strand, within Psyr_2346 at 2723.389 kb on + strand, within Psyr_2346 at 2723.389 kb on + strand, within Psyr_2346 at 2723.390 kb on - strand, within Psyr_2346 at 2723.396 kb on + strand, within Psyr_2346 at 2723.401 kb on - strand, within Psyr_2346 at 2723.401 kb on - strand, within Psyr_2346 at 2723.401 kb on - strand, within Psyr_2346 at 2723.442 kb on - strand, within Psyr_2346 at 2723.445 kb on + strand, within Psyr_2346 at 2723.446 kb on - strand, within Psyr_2346 at 2723.601 kb on + strand at 2723.602 kb on - strand at 2723.715 kb on + strand at 2723.761 kb on + strand at 2723.961 kb on - strand, within Psyr_2347 at 2723.970 kb on - strand, within Psyr_2347 at 2724.061 kb on - strand, within Psyr_2347 at 2724.128 kb on - strand, within Psyr_2347 at 2724.258 kb on - strand, within Psyr_2347 at 2724.258 kb on - strand, within Psyr_2347 at 2724.311 kb on + strand at 2724.471 kb on + strand at 2724.509 kb on - strand at 2724.627 kb on - strand at 2724.649 kb on + strand at 2724.677 kb on + strand at 2724.692 kb on + strand at 2724.849 kb on - strand, within Psyr_2348 at 2724.878 kb on - strand, within Psyr_2348 at 2725.195 kb on - strand, within Psyr_2348 at 2725.206 kb on + strand, within Psyr_2348 at 2725.207 kb on - strand, within Psyr_2348 at 2725.207 kb on - strand, within Psyr_2348 at 2725.220 kb on + strand, within Psyr_2348 at 2725.287 kb on + strand, within Psyr_2348 at 2725.288 kb on - strand, within Psyr_2348 at 2725.335 kb on + strand at 2725.339 kb on - strand at 2725.340 kb on + strand at 2725.340 kb on + strand at 2725.340 kb on + strand at 2725.341 kb on - strand at 2725.341 kb on - strand at 2725.345 kb on + strand at 2725.381 kb on - strand at 2725.387 kb on - strand at 2725.432 kb on + strand at 2725.467 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,722,854 + Psyr_2346 0.19 -2.7 2,722,854 + Psyr_2346 0.19 +2.7 2,722,923 - Psyr_2346 0.27 -3.9 2,722,923 - Psyr_2346 0.27 -5.0 2,722,990 - Psyr_2346 0.34 -3.8 2,723,070 + Psyr_2346 0.43 -0.1 2,723,070 + Psyr_2346 0.43 -0.1 2,723,075 - Psyr_2346 0.44 -1.8 2,723,121 + Psyr_2346 0.49 +0.6 2,723,121 + Psyr_2346 0.49 +2.2 2,723,221 + Psyr_2346 0.61 -0.1 2,723,222 - Psyr_2346 0.61 -1.0 2,723,389 + Psyr_2346 0.80 +0.9 2,723,389 + Psyr_2346 0.80 -2.7 2,723,390 - Psyr_2346 0.80 +1.9 2,723,396 + Psyr_2346 0.81 +2.2 2,723,401 - Psyr_2346 0.81 -0.1 2,723,401 - Psyr_2346 0.81 -5.2 2,723,401 - Psyr_2346 0.81 -0.1 2,723,442 - Psyr_2346 0.86 -0.4 2,723,445 + Psyr_2346 0.86 +0.9 2,723,446 - Psyr_2346 0.86 -0.1 2,723,601 + -0.1 2,723,602 - -0.4 2,723,715 + -0.1 2,723,761 + -3.9 2,723,961 - Psyr_2347 0.19 -3.1 2,723,970 - Psyr_2347 0.21 +0.1 2,724,061 - Psyr_2347 0.35 -2.4 2,724,128 - Psyr_2347 0.45 -2.4 2,724,258 - Psyr_2347 0.65 -3.8 2,724,258 - Psyr_2347 0.65 -2.9 2,724,311 + -0.1 2,724,471 + +1.1 2,724,509 - +0.9 2,724,627 - +2.7 2,724,649 + +3.3 2,724,677 + +0.9 2,724,692 + -2.9 2,724,849 - Psyr_2348 0.30 -3.1 2,724,878 - Psyr_2348 0.34 +1.1 2,725,195 - Psyr_2348 0.76 -0.1 2,725,206 + Psyr_2348 0.77 -3.1 2,725,207 - Psyr_2348 0.78 -0.1 2,725,207 - Psyr_2348 0.78 -0.1 2,725,220 + Psyr_2348 0.79 -2.8 2,725,287 + Psyr_2348 0.88 -0.1 2,725,288 - Psyr_2348 0.88 +0.2 2,725,335 + -0.3 2,725,339 - +0.1 2,725,340 + -1.5 2,725,340 + -0.1 2,725,340 + +3.7 2,725,341 - -2.6 2,725,341 - +4.0 2,725,345 + -1.2 2,725,381 - +0.9 2,725,387 - +4.6 2,725,432 + +3.3 2,725,467 - -2.0
Or see this region's nucleotide sequence