Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_2285

Experiment: KB with Acridine orange 62.5 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_2283 and Psyr_2284 are separated by 138 nucleotidesPsyr_2284 and Psyr_2285 are separated by 174 nucleotidesPsyr_2285 and Psyr_2286 overlap by 7 nucleotidesPsyr_2286 and Psyr_2287 are separated by 504 nucleotides Psyr_2283: Psyr_2283 - Secretion protein HlyD, at 2,653,659 to 2,654,819 _2283 Psyr_2284: Psyr_2284 - transcriptional regulator, LacI family, at 2,654,958 to 2,655,548 _2284 Psyr_2285: Psyr_2285 - Twin-arginine translocation pathway signal, at 2,655,723 to 2,656,328 _2285 Psyr_2286: Psyr_2286 - Protein of unknown function DUF461, at 2,656,322 to 2,656,816 _2286 Psyr_2287: Psyr_2287 - outer membrane transport energization protein TonB, at 2,657,321 to 2,658,154 _2287 Position (kb) 2655 2656 2657Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2654.781 kb on + strandat 2654.781 kb on + strandat 2654.813 kb on + strandat 2654.813 kb on + strandat 2654.814 kb on - strandat 2654.849 kb on - strandat 2654.924 kb on - strandat 2654.926 kb on - strandat 2654.976 kb on - strandat 2655.004 kb on + strandat 2655.004 kb on + strandat 2655.004 kb on + strandat 2655.005 kb on - strandat 2655.578 kb on + strandat 2655.579 kb on - strandat 2655.579 kb on - strandat 2655.662 kb on + strandat 2655.663 kb on - strandat 2656.201 kb on - strand, within Psyr_2285at 2656.201 kb on - strand, within Psyr_2285at 2656.201 kb on - strand, within Psyr_2285at 2656.227 kb on + strand, within Psyr_2285at 2656.228 kb on - strand, within Psyr_2285at 2656.321 kb on + strandat 2656.321 kb on + strandat 2656.321 kb on + strandat 2656.321 kb on + strandat 2656.322 kb on - strandat 2656.850 kb on - strandat 2656.861 kb on - strandat 2656.985 kb on + strandat 2656.985 kb on + strandat 2656.986 kb on - strandat 2656.986 kb on - strandat 2657.108 kb on - strandat 2657.172 kb on - strandat 2657.317 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml
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2,654,781 + +3.1
2,654,781 + -2.6
2,654,813 + -1.3
2,654,813 + +2.6
2,654,814 - -1.7
2,654,849 - -1.6
2,654,924 - -1.4
2,654,926 - -2.7
2,654,976 - +1.8
2,655,004 + -1.2
2,655,004 + +3.7
2,655,004 + +3.7
2,655,005 - -2.1
2,655,578 + +0.8
2,655,579 - +2.4
2,655,579 - +1.4
2,655,662 + -1.5
2,655,663 - +0.8
2,656,201 - Psyr_2285 0.79 +1.4
2,656,201 - Psyr_2285 0.79 -0.7
2,656,201 - Psyr_2285 0.79 -3.8
2,656,227 + Psyr_2285 0.83 -2.5
2,656,228 - Psyr_2285 0.83 -3.2
2,656,321 + -1.5
2,656,321 + +2.4
2,656,321 + -2.1
2,656,321 + +3.3
2,656,322 - +0.8
2,656,850 - +2.1
2,656,861 - +1.8
2,656,985 + -2.1
2,656,985 + +1.4
2,656,986 - +0.0
2,656,986 - +1.4
2,657,108 - -1.6
2,657,172 - +3.0
2,657,317 - -1.7

Or see this region's nucleotide sequence