Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2280 and Psyr_2281 are separated by 245 nucleotides Psyr_2281 and Psyr_2282 are separated by 21 nucleotides
Psyr_2280: Psyr_2280 - Alkanesulfonate monooxygenase, at 2,647,447 to 2,648,547
_2280
Psyr_2281: Psyr_2281 - GGDEF domain protein, at 2,648,793 to 2,650,460
_2281
Psyr_2282: Psyr_2282 - Hydrophobe/amphiphile efflux-1 HAE1, at 2,650,482 to 2,653,625
_2282
Position (kb)
2648
2649
2650
2651 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2647.794 kb on + strand, within Psyr_2280 at 2647.795 kb on - strand, within Psyr_2280 at 2647.795 kb on - strand, within Psyr_2280 at 2648.088 kb on + strand, within Psyr_2280 at 2648.301 kb on + strand, within Psyr_2280 at 2648.338 kb on + strand, within Psyr_2280 at 2648.359 kb on - strand, within Psyr_2280 at 2648.483 kb on + strand at 2648.483 kb on + strand at 2648.483 kb on + strand at 2648.484 kb on - strand at 2648.484 kb on - strand at 2648.652 kb on + strand at 2648.652 kb on + strand at 2648.652 kb on + strand at 2648.652 kb on + strand at 2648.652 kb on + strand at 2648.653 kb on - strand at 2648.653 kb on - strand at 2648.675 kb on + strand at 2648.675 kb on + strand at 2648.676 kb on - strand at 2648.685 kb on + strand at 2648.686 kb on - strand at 2648.686 kb on - strand at 2648.695 kb on + strand at 2648.696 kb on - strand at 2648.743 kb on + strand at 2649.021 kb on + strand, within Psyr_2281 at 2649.177 kb on + strand, within Psyr_2281 at 2649.177 kb on + strand, within Psyr_2281 at 2649.288 kb on + strand, within Psyr_2281 at 2649.335 kb on - strand, within Psyr_2281 at 2649.335 kb on - strand, within Psyr_2281 at 2649.356 kb on + strand, within Psyr_2281 at 2649.424 kb on - strand, within Psyr_2281 at 2649.424 kb on - strand, within Psyr_2281 at 2649.424 kb on - strand, within Psyr_2281 at 2649.432 kb on - strand, within Psyr_2281 at 2649.433 kb on + strand, within Psyr_2281 at 2649.433 kb on + strand, within Psyr_2281 at 2649.433 kb on + strand, within Psyr_2281 at 2649.433 kb on + strand, within Psyr_2281 at 2649.433 kb on + strand, within Psyr_2281 at 2649.434 kb on - strand, within Psyr_2281 at 2649.434 kb on - strand, within Psyr_2281 at 2649.434 kb on - strand, within Psyr_2281 at 2649.434 kb on - strand, within Psyr_2281 at 2649.434 kb on - strand, within Psyr_2281 at 2649.434 kb on - strand, within Psyr_2281 at 2649.434 kb on - strand, within Psyr_2281 at 2649.434 kb on - strand, within Psyr_2281 at 2649.794 kb on - strand, within Psyr_2281 at 2650.001 kb on + strand, within Psyr_2281 at 2650.321 kb on - strand at 2650.321 kb on - strand at 2650.321 kb on - strand at 2650.379 kb on + strand at 2650.379 kb on + strand at 2650.380 kb on - strand at 2650.380 kb on - strand at 2650.380 kb on - strand at 2650.861 kb on + strand, within Psyr_2282 at 2650.862 kb on - strand, within Psyr_2282 at 2650.963 kb on + strand at 2650.963 kb on + strand, within Psyr_2282 at 2650.963 kb on + strand, within Psyr_2282 at 2650.963 kb on + strand, within Psyr_2282 at 2650.963 kb on + strand, within Psyr_2282 at 2651.047 kb on + strand, within Psyr_2282 at 2651.047 kb on + strand, within Psyr_2282 at 2651.048 kb on - strand, within Psyr_2282 at 2651.048 kb on - strand, within Psyr_2282 at 2651.129 kb on + strand, within Psyr_2282 at 2651.269 kb on + strand, within Psyr_2282 at 2651.270 kb on - strand, within Psyr_2282
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,647,794 + Psyr_2280 0.32 -0.8 2,647,795 - Psyr_2280 0.32 +0.6 2,647,795 - Psyr_2280 0.32 -2.6 2,648,088 + Psyr_2280 0.58 +2.4 2,648,301 + Psyr_2280 0.78 +3.4 2,648,338 + Psyr_2280 0.81 +0.2 2,648,359 - Psyr_2280 0.83 +1.8 2,648,483 + +0.8 2,648,483 + -1.6 2,648,483 + +2.4 2,648,484 - +0.8 2,648,484 - +3.3 2,648,652 + +1.8 2,648,652 + +2.1 2,648,652 + -1.2 2,648,652 + -2.1 2,648,652 + -2.5 2,648,653 - +0.5 2,648,653 - +4.3 2,648,675 + +3.1 2,648,675 + -0.2 2,648,676 - +3.6 2,648,685 + +0.8 2,648,686 - +2.8 2,648,686 - -0.2 2,648,695 + +3.0 2,648,696 - +1.8 2,648,743 + +3.3 2,649,021 + Psyr_2281 0.14 +2.4 2,649,177 + Psyr_2281 0.23 +2.1 2,649,177 + Psyr_2281 0.23 +0.2 2,649,288 + Psyr_2281 0.30 -0.3 2,649,335 - Psyr_2281 0.32 +2.8 2,649,335 - Psyr_2281 0.32 +3.5 2,649,356 + Psyr_2281 0.34 +2.1 2,649,424 - Psyr_2281 0.38 +2.6 2,649,424 - Psyr_2281 0.38 -0.2 2,649,424 - Psyr_2281 0.38 +3.4 2,649,432 - Psyr_2281 0.38 +2.1 2,649,433 + Psyr_2281 0.38 -4.6 2,649,433 + Psyr_2281 0.38 +0.8 2,649,433 + Psyr_2281 0.38 +0.8 2,649,433 + Psyr_2281 0.38 -0.2 2,649,433 + Psyr_2281 0.38 +1.4 2,649,434 - Psyr_2281 0.38 -1.3 2,649,434 - Psyr_2281 0.38 +3.5 2,649,434 - Psyr_2281 0.38 +0.9 2,649,434 - Psyr_2281 0.38 -4.1 2,649,434 - Psyr_2281 0.38 -1.2 2,649,434 - Psyr_2281 0.38 +2.4 2,649,434 - Psyr_2281 0.38 +3.4 2,649,434 - Psyr_2281 0.38 -3.2 2,649,794 - Psyr_2281 0.60 -1.9 2,650,001 + Psyr_2281 0.72 +2.1 2,650,321 - +2.1 2,650,321 - +1.4 2,650,321 - +4.1 2,650,379 + -3.4 2,650,379 + -0.2 2,650,380 - +2.4 2,650,380 - +3.4 2,650,380 - -1.3 2,650,861 + Psyr_2282 0.12 +0.8 2,650,862 - Psyr_2282 0.12 -3.3 2,650,963 + -4.1 2,650,963 + Psyr_2282 0.15 -2.5 2,650,963 + Psyr_2282 0.15 -0.2 2,650,963 + Psyr_2282 0.15 -0.3 2,650,963 + Psyr_2282 0.15 -3.7 2,651,047 + Psyr_2282 0.18 -1.4 2,651,047 + Psyr_2282 0.18 -2.7 2,651,048 - Psyr_2282 0.18 +1.1 2,651,048 - Psyr_2282 0.18 -2.4 2,651,129 + Psyr_2282 0.21 +1.4 2,651,269 + Psyr_2282 0.25 -2.3 2,651,270 - Psyr_2282 0.25 -1.7
Or see this region's nucleotide sequence