Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2274 and Psyr_2275 overlap by 4 nucleotides Psyr_2275 and Psyr_2276 are separated by 15 nucleotides Psyr_2276 and Psyr_2277 are separated by 413 nucleotides
Psyr_2274: Psyr_2274 - glutamate synthase (NADPH) GltB1 subunit, at 2,640,302 to 2,641,222
_2274
Psyr_2275: Psyr_2275 - glutamate synthase (NADPH) GltB3 subunit, at 2,641,219 to 2,641,902
_2275
Psyr_2276: Psyr_2276 - glutamate synthase (NADPH) GltB2 subunit, at 2,641,918 to 2,643,258
_2276
Psyr_2277: Psyr_2277 - ammonium transporter, at 2,643,672 to 2,644,997
_2277
Position (kb)
2641
2642
2643
2644 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2641.003 kb on + strand, within Psyr_2274 at 2641.091 kb on + strand, within Psyr_2274 at 2641.091 kb on + strand, within Psyr_2274 at 2641.092 kb on - strand, within Psyr_2274 at 2641.092 kb on - strand, within Psyr_2274 at 2641.092 kb on - strand, within Psyr_2274 at 2641.092 kb on - strand, within Psyr_2274 at 2641.092 kb on - strand, within Psyr_2274 at 2641.181 kb on + strand at 2641.181 kb on + strand at 2641.181 kb on + strand at 2641.181 kb on + strand at 2641.181 kb on + strand at 2641.181 kb on + strand at 2641.181 kb on + strand at 2641.181 kb on + strand at 2641.182 kb on - strand at 2641.182 kb on - strand at 2641.182 kb on - strand at 2641.182 kb on - strand at 2641.182 kb on - strand at 2641.397 kb on - strand, within Psyr_2275 at 2641.397 kb on - strand, within Psyr_2275 at 2641.699 kb on + strand, within Psyr_2275 at 2641.700 kb on - strand, within Psyr_2275 at 2641.714 kb on + strand, within Psyr_2275 at 2641.715 kb on - strand, within Psyr_2275 at 2641.868 kb on - strand at 2641.914 kb on + strand at 2641.930 kb on - strand at 2642.023 kb on + strand at 2642.023 kb on + strand at 2642.023 kb on + strand at 2642.024 kb on - strand at 2642.179 kb on + strand, within Psyr_2276 at 2642.341 kb on + strand, within Psyr_2276 at 2642.347 kb on + strand, within Psyr_2276 at 2642.347 kb on + strand, within Psyr_2276 at 2642.490 kb on + strand, within Psyr_2276 at 2642.490 kb on + strand, within Psyr_2276 at 2642.490 kb on + strand, within Psyr_2276 at 2642.490 kb on + strand, within Psyr_2276 at 2642.490 kb on + strand, within Psyr_2276 at 2642.611 kb on + strand, within Psyr_2276 at 2642.611 kb on + strand, within Psyr_2276 at 2642.611 kb on + strand, within Psyr_2276 at 2642.641 kb on + strand, within Psyr_2276 at 2642.641 kb on + strand, within Psyr_2276 at 2642.641 kb on + strand, within Psyr_2276 at 2642.642 kb on - strand, within Psyr_2276 at 2642.980 kb on + strand, within Psyr_2276 at 2642.981 kb on - strand, within Psyr_2276 at 2643.085 kb on + strand, within Psyr_2276 at 2643.086 kb on - strand, within Psyr_2276 at 2643.086 kb on - strand, within Psyr_2276 at 2643.086 kb on - strand, within Psyr_2276 at 2643.086 kb on - strand, within Psyr_2276 at 2643.562 kb on + strand at 2643.562 kb on + strand at 2643.563 kb on - strand at 2643.569 kb on - strand at 2643.686 kb on - strand at 2643.710 kb on - strand at 2643.933 kb on + strand, within Psyr_2277 at 2643.933 kb on + strand, within Psyr_2277 at 2643.933 kb on + strand, within Psyr_2277 at 2643.934 kb on - strand at 2643.934 kb on - strand, within Psyr_2277 at 2643.934 kb on - strand, within Psyr_2277 at 2643.940 kb on - strand, within Psyr_2277 at 2644.194 kb on + strand, within Psyr_2277 at 2644.195 kb on - strand, within Psyr_2277 at 2644.195 kb on - strand, within Psyr_2277 at 2644.195 kb on - strand, within Psyr_2277
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,641,003 + Psyr_2274 0.76 +1.4 2,641,091 + Psyr_2274 0.86 +3.0 2,641,091 + Psyr_2274 0.86 +3.4 2,641,092 - Psyr_2274 0.86 -1.3 2,641,092 - Psyr_2274 0.86 +3.5 2,641,092 - Psyr_2274 0.86 -2.9 2,641,092 - Psyr_2274 0.86 -1.9 2,641,092 - Psyr_2274 0.86 +2.1 2,641,181 + -0.8 2,641,181 + -0.5 2,641,181 + -1.1 2,641,181 + +3.3 2,641,181 + -2.2 2,641,181 + +3.1 2,641,181 + +3.1 2,641,181 + -0.3 2,641,182 - +3.0 2,641,182 - -2.1 2,641,182 - -2.7 2,641,182 - -0.8 2,641,182 - -1.5 2,641,397 - Psyr_2275 0.26 +3.0 2,641,397 - Psyr_2275 0.26 -1.6 2,641,699 + Psyr_2275 0.70 -2.7 2,641,700 - Psyr_2275 0.70 -1.5 2,641,714 + Psyr_2275 0.72 +0.3 2,641,715 - Psyr_2275 0.73 +1.4 2,641,868 - +3.5 2,641,914 + +3.0 2,641,930 - -1.5 2,642,023 + -0.1 2,642,023 + -1.4 2,642,023 + -0.8 2,642,024 - +2.4 2,642,179 + Psyr_2276 0.19 +4.1 2,642,341 + Psyr_2276 0.32 +2.1 2,642,347 + Psyr_2276 0.32 -2.2 2,642,347 + Psyr_2276 0.32 +3.0 2,642,490 + Psyr_2276 0.43 +2.6 2,642,490 + Psyr_2276 0.43 -1.5 2,642,490 + Psyr_2276 0.43 -1.7 2,642,490 + Psyr_2276 0.43 -1.8 2,642,490 + Psyr_2276 0.43 -0.3 2,642,611 + Psyr_2276 0.52 -1.2 2,642,611 + Psyr_2276 0.52 -1.7 2,642,611 + Psyr_2276 0.52 -1.4 2,642,641 + Psyr_2276 0.54 -3.7 2,642,641 + Psyr_2276 0.54 +4.0 2,642,641 + Psyr_2276 0.54 +2.4 2,642,642 - Psyr_2276 0.54 -3.0 2,642,980 + Psyr_2276 0.79 +2.4 2,642,981 - Psyr_2276 0.79 +0.5 2,643,085 + Psyr_2276 0.87 -0.2 2,643,086 - Psyr_2276 0.87 -1.5 2,643,086 - Psyr_2276 0.87 -0.2 2,643,086 - Psyr_2276 0.87 +1.8 2,643,086 - Psyr_2276 0.87 +0.8 2,643,562 + -1.5 2,643,562 + -5.5 2,643,563 - +2.6 2,643,569 - +0.9 2,643,686 - +3.0 2,643,710 - -1.0 2,643,933 + Psyr_2277 0.20 +2.4 2,643,933 + Psyr_2277 0.20 -1.1 2,643,933 + Psyr_2277 0.20 +1.1 2,643,934 - -4.1 2,643,934 - Psyr_2277 0.20 +2.1 2,643,934 - Psyr_2277 0.20 -1.8 2,643,940 - Psyr_2277 0.20 -2.1 2,644,194 + Psyr_2277 0.39 +3.4 2,644,195 - Psyr_2277 0.39 +1.4 2,644,195 - Psyr_2277 0.39 +1.8 2,644,195 - Psyr_2277 0.39 -1.4
Or see this region's nucleotide sequence