Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2268 and Psyr_2269 are separated by 174 nucleotides Psyr_2269 and Psyr_2270 are separated by 11 nucleotides Psyr_2270 and Psyr_2271 are separated by 140 nucleotides
Psyr_2268: Psyr_2268 - transcriptional regulator, LysR family, at 2,634,007 to 2,634,915
_2268
Psyr_2269: Psyr_2269 - GTP cyclohydrolase I, at 2,635,090 to 2,635,995
_2269
Psyr_2270: Psyr_2270 - transferase hexapeptide repeat protein, at 2,636,007 to 2,636,567
_2270
Psyr_2271: Psyr_2271 - membrane protein, putative, at 2,636,708 to 2,637,874
_2271
Position (kb)
2635
2636 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2634.535 kb on - strand, within Psyr_2268 at 2634.586 kb on + strand, within Psyr_2268 at 2634.586 kb on + strand, within Psyr_2268 at 2634.586 kb on + strand, within Psyr_2268 at 2634.586 kb on + strand, within Psyr_2268 at 2634.587 kb on - strand, within Psyr_2268 at 2634.636 kb on + strand, within Psyr_2268 at 2634.636 kb on + strand, within Psyr_2268 at 2634.689 kb on - strand, within Psyr_2268 at 2634.689 kb on - strand, within Psyr_2268 at 2634.689 kb on - strand, within Psyr_2268 at 2634.811 kb on - strand, within Psyr_2268 at 2634.811 kb on - strand, within Psyr_2268 at 2634.950 kb on + strand at 2634.950 kb on + strand at 2634.950 kb on + strand at 2634.950 kb on + strand at 2634.951 kb on - strand at 2634.951 kb on - strand at 2634.954 kb on + strand at 2634.955 kb on - strand at 2634.955 kb on - strand at 2635.020 kb on + strand at 2635.020 kb on + strand at 2635.021 kb on - strand at 2635.275 kb on + strand, within Psyr_2269 at 2635.275 kb on + strand, within Psyr_2269 at 2635.275 kb on + strand, within Psyr_2269 at 2635.275 kb on + strand, within Psyr_2269 at 2635.276 kb on - strand, within Psyr_2269 at 2635.276 kb on - strand, within Psyr_2269 at 2635.543 kb on + strand, within Psyr_2269 at 2635.773 kb on - strand, within Psyr_2269 at 2635.861 kb on + strand, within Psyr_2269 at 2635.861 kb on + strand, within Psyr_2269 at 2635.861 kb on + strand, within Psyr_2269 at 2635.861 kb on + strand, within Psyr_2269 at 2635.984 kb on + strand at 2635.984 kb on + strand at 2635.985 kb on - strand at 2635.985 kb on - strand at 2636.059 kb on - strand at 2636.059 kb on - strand at 2636.067 kb on - strand, within Psyr_2270 at 2636.418 kb on + strand, within Psyr_2270 at 2636.418 kb on + strand, within Psyr_2270 at 2636.418 kb on + strand, within Psyr_2270 at 2636.418 kb on + strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.419 kb on - strand, within Psyr_2270 at 2636.598 kb on + strand at 2636.600 kb on + strand at 2636.600 kb on + strand at 2636.600 kb on + strand at 2636.601 kb on - strand at 2636.601 kb on - strand at 2636.601 kb on - strand at 2636.613 kb on - strand at 2636.668 kb on + strand at 2636.669 kb on - strand at 2636.669 kb on - strand at 2636.782 kb on + strand at 2636.782 kb on + strand at 2636.963 kb on + strand, within Psyr_2271 at 2636.964 kb on - strand, within Psyr_2271
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,634,535 - Psyr_2268 0.58 +1.8 2,634,586 + Psyr_2268 0.64 -0.2 2,634,586 + Psyr_2268 0.64 +1.4 2,634,586 + Psyr_2268 0.64 -0.9 2,634,586 + Psyr_2268 0.64 +1.6 2,634,587 - Psyr_2268 0.64 +2.8 2,634,636 + Psyr_2268 0.69 +0.1 2,634,636 + Psyr_2268 0.69 +0.7 2,634,689 - Psyr_2268 0.75 +1.4 2,634,689 - Psyr_2268 0.75 -0.2 2,634,689 - Psyr_2268 0.75 -2.5 2,634,811 - Psyr_2268 0.88 +0.8 2,634,811 - Psyr_2268 0.88 -3.6 2,634,950 + -1.9 2,634,950 + +3.4 2,634,950 + -0.9 2,634,950 + +2.1 2,634,951 - -5.2 2,634,951 - +2.1 2,634,954 + -1.4 2,634,955 - -4.4 2,634,955 - -2.8 2,635,020 + +2.8 2,635,020 + -1.6 2,635,021 - -2.6 2,635,275 + Psyr_2269 0.20 -1.3 2,635,275 + Psyr_2269 0.20 -0.9 2,635,275 + Psyr_2269 0.20 -0.1 2,635,275 + Psyr_2269 0.20 +1.8 2,635,276 - Psyr_2269 0.21 -0.2 2,635,276 - Psyr_2269 0.21 -0.8 2,635,543 + Psyr_2269 0.50 +1.1 2,635,773 - Psyr_2269 0.75 +1.4 2,635,861 + Psyr_2269 0.85 +3.4 2,635,861 + Psyr_2269 0.85 +3.4 2,635,861 + Psyr_2269 0.85 +3.6 2,635,861 + Psyr_2269 0.85 +2.1 2,635,984 + +3.0 2,635,984 + +2.8 2,635,985 - +3.1 2,635,985 - +1.8 2,636,059 - -1.4 2,636,059 - +0.4 2,636,067 - Psyr_2270 0.11 +1.4 2,636,418 + Psyr_2270 0.73 -0.2 2,636,418 + Psyr_2270 0.73 -3.9 2,636,418 + Psyr_2270 0.73 +1.8 2,636,418 + Psyr_2270 0.73 +1.8 2,636,419 - Psyr_2270 0.73 -3.4 2,636,419 - Psyr_2270 0.73 +0.8 2,636,419 - Psyr_2270 0.73 -0.2 2,636,419 - Psyr_2270 0.73 +2.1 2,636,419 - Psyr_2270 0.73 -0.8 2,636,419 - Psyr_2270 0.73 +3.7 2,636,598 + -2.4 2,636,600 + -3.2 2,636,600 + +3.6 2,636,600 + +2.4 2,636,601 - +3.4 2,636,601 - +1.0 2,636,601 - +2.1 2,636,613 - +0.8 2,636,668 + -0.8 2,636,669 - -0.0 2,636,669 - +2.6 2,636,782 + -2.2 2,636,782 + -1.0 2,636,963 + Psyr_2271 0.22 +2.1 2,636,964 - Psyr_2271 0.22 +0.8
Or see this region's nucleotide sequence