Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2258 and Psyr_2259 overlap by 10 nucleotides Psyr_2259 and Psyr_2260 are separated by 9 nucleotides Psyr_2260 and Psyr_2261 are separated by 118 nucleotides
Psyr_2258: Psyr_2258 - Guanylate kinase/L-type calcium channel region, at 2,622,773 to 2,623,339
_2258
Psyr_2259: Psyr_2259 - Beta-lactamase-like protein, at 2,623,330 to 2,624,082
_2259
Psyr_2260: Psyr_2260 - Amidase, at 2,624,092 to 2,625,615
_2260
Psyr_2261: Psyr_2261 - Peptidase M20:Peptidase M20, at 2,625,734 to 2,626,885
_2261
Position (kb)
2624
2625
2626 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2623.267 kb on + strand, within Psyr_2258 at 2623.378 kb on + strand at 2623.552 kb on + strand, within Psyr_2259 at 2623.846 kb on + strand, within Psyr_2259 at 2623.998 kb on + strand, within Psyr_2259 at 2623.999 kb on - strand, within Psyr_2259 at 2624.048 kb on + strand at 2624.048 kb on + strand at 2624.048 kb on + strand at 2624.048 kb on + strand at 2624.049 kb on - strand at 2624.067 kb on + strand at 2624.068 kb on - strand at 2624.402 kb on + strand, within Psyr_2260 at 2624.402 kb on + strand, within Psyr_2260 at 2624.402 kb on + strand, within Psyr_2260 at 2624.403 kb on - strand, within Psyr_2260 at 2624.403 kb on - strand, within Psyr_2260 at 2624.403 kb on - strand, within Psyr_2260 at 2624.403 kb on - strand at 2624.472 kb on + strand, within Psyr_2260 at 2624.495 kb on + strand, within Psyr_2260 at 2624.495 kb on + strand, within Psyr_2260 at 2624.880 kb on + strand, within Psyr_2260 at 2624.880 kb on + strand, within Psyr_2260 at 2624.881 kb on - strand, within Psyr_2260 at 2624.882 kb on + strand, within Psyr_2260 at 2624.882 kb on + strand, within Psyr_2260 at 2624.882 kb on + strand, within Psyr_2260 at 2624.882 kb on + strand, within Psyr_2260 at 2624.883 kb on - strand, within Psyr_2260 at 2624.883 kb on - strand, within Psyr_2260 at 2624.965 kb on + strand, within Psyr_2260 at 2625.253 kb on + strand, within Psyr_2260 at 2625.333 kb on - strand, within Psyr_2260 at 2625.333 kb on - strand, within Psyr_2260 at 2625.636 kb on + strand at 2625.637 kb on - strand at 2625.637 kb on - strand at 2625.637 kb on - strand at 2625.640 kb on + strand at 2625.640 kb on + strand at 2625.640 kb on + strand at 2625.640 kb on + strand at 2625.640 kb on + strand at 2625.640 kb on + strand at 2625.641 kb on - strand at 2625.641 kb on - strand at 2625.659 kb on + strand at 2625.659 kb on + strand at 2625.954 kb on + strand, within Psyr_2261 at 2625.955 kb on - strand, within Psyr_2261 at 2625.955 kb on - strand, within Psyr_2261 at 2625.958 kb on + strand, within Psyr_2261 at 2625.958 kb on + strand, within Psyr_2261 at 2625.959 kb on - strand, within Psyr_2261 at 2625.959 kb on - strand, within Psyr_2261 at 2626.076 kb on + strand, within Psyr_2261 at 2626.145 kb on - strand, within Psyr_2261 at 2626.226 kb on + strand, within Psyr_2261 at 2626.227 kb on - strand, within Psyr_2261 at 2626.401 kb on + strand, within Psyr_2261 at 2626.455 kb on + strand, within Psyr_2261 at 2626.456 kb on - strand, within Psyr_2261
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,623,267 + Psyr_2258 0.87 -1.5 2,623,378 + +3.7 2,623,552 + Psyr_2259 0.29 +1.9 2,623,846 + Psyr_2259 0.69 +2.7 2,623,998 + Psyr_2259 0.89 +3.5 2,623,999 - Psyr_2259 0.89 -0.7 2,624,048 + -0.4 2,624,048 + +2.5 2,624,048 + -2.2 2,624,048 + -0.6 2,624,049 - -1.4 2,624,067 + -3.9 2,624,068 - +0.4 2,624,402 + Psyr_2260 0.20 +0.9 2,624,402 + Psyr_2260 0.20 -1.8 2,624,402 + Psyr_2260 0.20 +2.7 2,624,403 - Psyr_2260 0.20 -2.7 2,624,403 - Psyr_2260 0.20 -1.5 2,624,403 - Psyr_2260 0.20 -3.2 2,624,403 - -0.1 2,624,472 + Psyr_2260 0.25 +2.2 2,624,495 + Psyr_2260 0.26 +2.2 2,624,495 + Psyr_2260 0.26 -1.4 2,624,880 + Psyr_2260 0.52 -1.5 2,624,880 + Psyr_2260 0.52 +2.7 2,624,881 - Psyr_2260 0.52 -2.2 2,624,882 + Psyr_2260 0.52 +2.5 2,624,882 + Psyr_2260 0.52 -1.9 2,624,882 + Psyr_2260 0.52 -0.1 2,624,882 + Psyr_2260 0.52 -3.6 2,624,883 - Psyr_2260 0.52 +3.7 2,624,883 - Psyr_2260 0.52 +0.9 2,624,965 + Psyr_2260 0.57 +1.9 2,625,253 + Psyr_2260 0.76 +2.9 2,625,333 - Psyr_2260 0.81 +2.5 2,625,333 - Psyr_2260 0.81 +3.6 2,625,636 + +2.2 2,625,637 - -2.2 2,625,637 - +3.7 2,625,637 - +3.2 2,625,640 + -1.1 2,625,640 + -0.0 2,625,640 + +2.7 2,625,640 + +3.2 2,625,640 + -3.1 2,625,640 + +3.0 2,625,641 - +3.9 2,625,641 - +2.7 2,625,659 + -0.4 2,625,659 + -0.4 2,625,954 + Psyr_2261 0.19 +3.6 2,625,955 - Psyr_2261 0.19 +1.9 2,625,955 - Psyr_2261 0.19 -0.7 2,625,958 + Psyr_2261 0.19 +2.2 2,625,958 + Psyr_2261 0.19 +3.3 2,625,959 - Psyr_2261 0.20 +1.3 2,625,959 - Psyr_2261 0.20 +0.7 2,626,076 + Psyr_2261 0.30 -1.1 2,626,145 - Psyr_2261 0.36 +2.7 2,626,226 + Psyr_2261 0.43 -1.7 2,626,227 - Psyr_2261 0.43 +3.3 2,626,401 + Psyr_2261 0.58 -0.4 2,626,455 + Psyr_2261 0.63 -1.0 2,626,456 - Psyr_2261 0.63 -0.4
Or see this region's nucleotide sequence