Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2257 and Psyr_2258 overlap by 1 nucleotides Psyr_2258 and Psyr_2259 overlap by 10 nucleotides Psyr_2259 and Psyr_2260 are separated by 9 nucleotides
Psyr_2257: Psyr_2257 - Amidohydrolase, at 2,621,628 to 2,622,773
_2257
Psyr_2258: Psyr_2258 - Guanylate kinase/L-type calcium channel region, at 2,622,773 to 2,623,339
_2258
Psyr_2259: Psyr_2259 - Beta-lactamase-like protein, at 2,623,330 to 2,624,082
_2259
Psyr_2260: Psyr_2260 - Amidase, at 2,624,092 to 2,625,615
_2260
Position (kb)
2623
2624
2625 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2622.543 kb on + strand at 2622.676 kb on + strand at 2622.677 kb on - strand at 2622.677 kb on - strand at 2622.677 kb on - strand at 2622.677 kb on - strand at 2622.677 kb on - strand at 2622.677 kb on - strand at 2622.791 kb on + strand at 2622.791 kb on + strand at 2622.791 kb on + strand at 2622.792 kb on - strand at 2622.792 kb on - strand at 2623.267 kb on + strand, within Psyr_2258 at 2623.378 kb on + strand at 2623.552 kb on + strand, within Psyr_2259 at 2623.846 kb on + strand, within Psyr_2259 at 2623.998 kb on + strand, within Psyr_2259 at 2623.999 kb on - strand, within Psyr_2259 at 2624.048 kb on + strand at 2624.048 kb on + strand at 2624.048 kb on + strand at 2624.048 kb on + strand at 2624.049 kb on - strand at 2624.067 kb on + strand at 2624.068 kb on - strand at 2624.402 kb on + strand, within Psyr_2260 at 2624.402 kb on + strand, within Psyr_2260 at 2624.402 kb on + strand, within Psyr_2260 at 2624.403 kb on - strand, within Psyr_2260 at 2624.403 kb on - strand, within Psyr_2260 at 2624.403 kb on - strand, within Psyr_2260 at 2624.403 kb on - strand at 2624.472 kb on + strand, within Psyr_2260 at 2624.495 kb on + strand, within Psyr_2260 at 2624.495 kb on + strand, within Psyr_2260 at 2624.880 kb on + strand, within Psyr_2260 at 2624.880 kb on + strand, within Psyr_2260 at 2624.881 kb on - strand, within Psyr_2260 at 2624.882 kb on + strand, within Psyr_2260 at 2624.882 kb on + strand, within Psyr_2260 at 2624.882 kb on + strand, within Psyr_2260 at 2624.882 kb on + strand, within Psyr_2260 at 2624.883 kb on - strand, within Psyr_2260 at 2624.883 kb on - strand, within Psyr_2260 at 2624.965 kb on + strand, within Psyr_2260
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,622,543 + +0.9 2,622,676 + +1.9 2,622,677 - +2.2 2,622,677 - +1.9 2,622,677 - -2.3 2,622,677 - +3.7 2,622,677 - +1.9 2,622,677 - +3.0 2,622,791 + +1.9 2,622,791 + +3.8 2,622,791 + -0.0 2,622,792 - -3.5 2,622,792 - -4.4 2,623,267 + Psyr_2258 0.87 -1.5 2,623,378 + +3.7 2,623,552 + Psyr_2259 0.29 +1.9 2,623,846 + Psyr_2259 0.69 +2.7 2,623,998 + Psyr_2259 0.89 +3.5 2,623,999 - Psyr_2259 0.89 -0.7 2,624,048 + -0.4 2,624,048 + +2.5 2,624,048 + -2.2 2,624,048 + -0.6 2,624,049 - -1.4 2,624,067 + -3.9 2,624,068 - +0.4 2,624,402 + Psyr_2260 0.20 +0.9 2,624,402 + Psyr_2260 0.20 -1.8 2,624,402 + Psyr_2260 0.20 +2.7 2,624,403 - Psyr_2260 0.20 -2.7 2,624,403 - Psyr_2260 0.20 -1.5 2,624,403 - Psyr_2260 0.20 -3.2 2,624,403 - -0.1 2,624,472 + Psyr_2260 0.25 +2.2 2,624,495 + Psyr_2260 0.26 +2.2 2,624,495 + Psyr_2260 0.26 -1.4 2,624,880 + Psyr_2260 0.52 -1.5 2,624,880 + Psyr_2260 0.52 +2.7 2,624,881 - Psyr_2260 0.52 -2.2 2,624,882 + Psyr_2260 0.52 +2.5 2,624,882 + Psyr_2260 0.52 -1.9 2,624,882 + Psyr_2260 0.52 -0.1 2,624,882 + Psyr_2260 0.52 -3.6 2,624,883 - Psyr_2260 0.52 +3.7 2,624,883 - Psyr_2260 0.52 +0.9 2,624,965 + Psyr_2260 0.57 +1.9
Or see this region's nucleotide sequence