Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2230 and Psyr_2231 are separated by 160 nucleotides Psyr_2231 and Psyr_2232 are separated by 6 nucleotides Psyr_2232 and Psyr_2233 are separated by 60 nucleotides Psyr_2233 and Psyr_2234 are separated by 2 nucleotides Psyr_2234 and Psyr_2235 are separated by 190 nucleotides
Psyr_2230: Psyr_2230 - Mn2+/Fe2+ transporter, NRAMP family, at 2,592,853 to 2,594,154
_2230
Psyr_2231: Psyr_2231 - Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase, at 2,594,315 to 2,595,064
_2231
Psyr_2232: Psyr_2232 - Major facilitator superfamily, at 2,595,071 to 2,596,369
_2232
Psyr_2233: Psyr_2233 - Protein of unknown function DUF1289, at 2,596,430 to 2,596,612
_2233
Psyr_2234: Psyr_2234 - conserved hypothetical protein, at 2,596,615 to 2,596,986
_2234
Psyr_2235: Psyr_2235 - gluconolactonase, at 2,597,177 to 2,598,277
_2235
Position (kb)
2595
2596
2597 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2594.179 kb on + strand at 2594.179 kb on + strand at 2594.180 kb on - strand at 2594.246 kb on - strand at 2594.246 kb on - strand at 2594.249 kb on + strand at 2594.249 kb on + strand at 2594.249 kb on + strand at 2594.250 kb on - strand at 2594.250 kb on - strand at 2594.276 kb on + strand at 2594.654 kb on + strand, within Psyr_2231 at 2594.655 kb on - strand, within Psyr_2231 at 2594.705 kb on + strand, within Psyr_2231 at 2594.706 kb on - strand, within Psyr_2231 at 2594.804 kb on + strand, within Psyr_2231 at 2594.843 kb on + strand, within Psyr_2231 at 2594.843 kb on + strand, within Psyr_2231 at 2594.844 kb on - strand, within Psyr_2231 at 2594.844 kb on - strand, within Psyr_2231 at 2594.844 kb on - strand, within Psyr_2231 at 2595.420 kb on + strand, within Psyr_2232 at 2595.420 kb on + strand, within Psyr_2232 at 2595.420 kb on + strand, within Psyr_2232 at 2595.421 kb on - strand, within Psyr_2232 at 2595.822 kb on + strand, within Psyr_2232 at 2595.823 kb on - strand, within Psyr_2232 at 2595.835 kb on + strand, within Psyr_2232 at 2595.836 kb on - strand, within Psyr_2232 at 2596.224 kb on + strand, within Psyr_2232 at 2596.224 kb on + strand, within Psyr_2232 at 2596.224 kb on + strand, within Psyr_2232 at 2596.225 kb on - strand, within Psyr_2232 at 2596.225 kb on - strand, within Psyr_2232 at 2596.237 kb on - strand, within Psyr_2232 at 2596.340 kb on - strand at 2596.374 kb on - strand at 2596.377 kb on - strand at 2596.377 kb on - strand at 2596.394 kb on + strand at 2596.424 kb on + strand at 2597.025 kb on - strand at 2597.025 kb on - strand at 2597.062 kb on + strand at 2597.062 kb on + strand at 2597.062 kb on + strand at 2597.064 kb on + strand at 2597.064 kb on + strand at 2597.064 kb on + strand at 2597.065 kb on - strand at 2597.117 kb on + strand at 2597.124 kb on + strand at 2597.124 kb on + strand at 2597.124 kb on + strand at 2597.125 kb on - strand at 2597.125 kb on - strand at 2597.165 kb on + strand at 2597.166 kb on - strand at 2597.171 kb on + strand at 2597.172 kb on - strand at 2597.363 kb on - strand, within Psyr_2235
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,594,179 + -2.2 2,594,179 + +2.2 2,594,180 - +3.3 2,594,246 - -0.1 2,594,246 - -2.9 2,594,249 + -0.1 2,594,249 + +3.8 2,594,249 + +2.9 2,594,250 - -3.8 2,594,250 - -2.8 2,594,276 + -2.0 2,594,654 + Psyr_2231 0.45 +3.0 2,594,655 - Psyr_2231 0.45 -0.1 2,594,705 + Psyr_2231 0.52 +2.9 2,594,706 - Psyr_2231 0.52 -0.8 2,594,804 + Psyr_2231 0.65 -0.2 2,594,843 + Psyr_2231 0.70 +1.5 2,594,843 + Psyr_2231 0.70 +2.5 2,594,844 - Psyr_2231 0.71 +1.9 2,594,844 - Psyr_2231 0.71 -0.7 2,594,844 - Psyr_2231 0.71 -0.9 2,595,420 + Psyr_2232 0.27 -0.8 2,595,420 + Psyr_2232 0.27 +0.9 2,595,420 + Psyr_2232 0.27 -0.9 2,595,421 - Psyr_2232 0.27 -2.2 2,595,822 + Psyr_2232 0.58 -0.0 2,595,823 - Psyr_2232 0.58 +0.1 2,595,835 + Psyr_2232 0.59 -1.1 2,595,836 - Psyr_2232 0.59 +1.9 2,596,224 + Psyr_2232 0.89 -0.5 2,596,224 + Psyr_2232 0.89 -0.6 2,596,224 + Psyr_2232 0.89 -2.3 2,596,225 - Psyr_2232 0.89 -2.0 2,596,225 - Psyr_2232 0.89 +1.5 2,596,237 - Psyr_2232 0.90 -2.2 2,596,340 - -1.6 2,596,374 - -2.2 2,596,377 - -1.3 2,596,377 - -2.3 2,596,394 + -2.1 2,596,424 + +0.2 2,597,025 - +1.5 2,597,025 - +2.9 2,597,062 + -1.3 2,597,062 + +3.1 2,597,062 + +3.7 2,597,064 + +2.2 2,597,064 + -0.8 2,597,064 + +0.7 2,597,065 - -0.1 2,597,117 + -0.4 2,597,124 + +3.3 2,597,124 + -1.0 2,597,124 + +1.9 2,597,125 - +2.5 2,597,125 - +2.2 2,597,165 + +3.1 2,597,166 - -0.5 2,597,171 + -0.6 2,597,172 - +2.7 2,597,363 - Psyr_2235 0.17 -2.1
Or see this region's nucleotide sequence