Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2228 and Psyr_2229 are separated by 53 nucleotides Psyr_2229 and Psyr_2230 are separated by 188 nucleotides
Psyr_2228: Psyr_2228 - conserved hypothetical protein, at 2,589,771 to 2,590,499
_2228
Psyr_2229: Psyr_2229 - TonB-dependent siderophore receptor, at 2,590,553 to 2,592,664
_2229
Psyr_2230: Psyr_2230 - Mn2+/Fe2+ transporter, NRAMP family, at 2,592,853 to 2,594,154
_2230
Position (kb)
2590
2591
2592
2593 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2589.728 kb on + strand at 2589.729 kb on - strand at 2589.729 kb on - strand at 2590.111 kb on + strand, within Psyr_2228 at 2590.112 kb on - strand, within Psyr_2228 at 2590.112 kb on - strand, within Psyr_2228 at 2590.112 kb on - strand, within Psyr_2228 at 2590.112 kb on - strand, within Psyr_2228 at 2590.126 kb on + strand, within Psyr_2228 at 2590.126 kb on + strand, within Psyr_2228 at 2590.127 kb on - strand, within Psyr_2228 at 2590.136 kb on + strand, within Psyr_2228 at 2590.136 kb on + strand, within Psyr_2228 at 2590.137 kb on - strand, within Psyr_2228 at 2590.137 kb on - strand, within Psyr_2228 at 2590.325 kb on - strand, within Psyr_2228 at 2590.530 kb on + strand at 2590.531 kb on - strand at 2590.580 kb on - strand at 2590.611 kb on + strand at 2590.611 kb on + strand at 2590.611 kb on + strand at 2590.612 kb on - strand at 2590.712 kb on + strand at 2590.773 kb on + strand, within Psyr_2229 at 2590.773 kb on + strand, within Psyr_2229 at 2590.774 kb on - strand, within Psyr_2229 at 2590.779 kb on + strand, within Psyr_2229 at 2590.827 kb on + strand, within Psyr_2229 at 2590.828 kb on - strand, within Psyr_2229 at 2590.828 kb on - strand, within Psyr_2229 at 2590.923 kb on + strand, within Psyr_2229 at 2591.331 kb on + strand, within Psyr_2229 at 2591.416 kb on - strand, within Psyr_2229 at 2591.472 kb on + strand, within Psyr_2229 at 2591.472 kb on + strand, within Psyr_2229 at 2591.472 kb on + strand, within Psyr_2229 at 2591.472 kb on + strand, within Psyr_2229 at 2591.472 kb on + strand, within Psyr_2229 at 2591.472 kb on + strand, within Psyr_2229 at 2591.472 kb on + strand, within Psyr_2229 at 2591.473 kb on - strand, within Psyr_2229 at 2591.473 kb on - strand, within Psyr_2229 at 2591.473 kb on - strand, within Psyr_2229 at 2591.473 kb on - strand, within Psyr_2229 at 2591.473 kb on - strand, within Psyr_2229 at 2591.530 kb on - strand, within Psyr_2229 at 2591.785 kb on + strand, within Psyr_2229 at 2591.785 kb on + strand, within Psyr_2229 at 2591.786 kb on - strand, within Psyr_2229 at 2591.984 kb on + strand, within Psyr_2229 at 2591.984 kb on + strand, within Psyr_2229 at 2591.984 kb on + strand, within Psyr_2229 at 2591.985 kb on - strand, within Psyr_2229 at 2591.985 kb on - strand, within Psyr_2229 at 2591.985 kb on - strand, within Psyr_2229 at 2591.985 kb on - strand, within Psyr_2229 at 2592.124 kb on + strand, within Psyr_2229 at 2592.223 kb on + strand, within Psyr_2229 at 2592.224 kb on - strand, within Psyr_2229 at 2592.233 kb on - strand, within Psyr_2229 at 2592.288 kb on + strand, within Psyr_2229 at 2592.288 kb on + strand, within Psyr_2229 at 2592.288 kb on + strand, within Psyr_2229 at 2592.289 kb on - strand, within Psyr_2229 at 2592.328 kb on - strand, within Psyr_2229 at 2592.349 kb on + strand, within Psyr_2229 at 2592.741 kb on + strand at 2592.741 kb on + strand at 2592.742 kb on - strand at 2592.754 kb on + strand at 2592.793 kb on + strand at 2592.794 kb on - strand at 2592.947 kb on + strand at 2592.948 kb on - strand at 2592.988 kb on + strand, within Psyr_2230 at 2593.021 kb on - strand, within Psyr_2230 at 2593.271 kb on + strand, within Psyr_2230 at 2593.271 kb on + strand, within Psyr_2230 at 2593.271 kb on + strand, within Psyr_2230 at 2593.271 kb on + strand, within Psyr_2230 at 2593.271 kb on + strand, within Psyr_2230 at 2593.271 kb on + strand, within Psyr_2230 at 2593.271 kb on + strand, within Psyr_2230 at 2593.272 kb on - strand, within Psyr_2230 at 2593.272 kb on - strand, within Psyr_2230 at 2593.272 kb on - strand, within Psyr_2230 at 2593.478 kb on + strand, within Psyr_2230 at 2593.479 kb on - strand, within Psyr_2230 at 2593.495 kb on - strand, within Psyr_2230 at 2593.510 kb on + strand, within Psyr_2230
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,589,728 + -0.9 2,589,729 - +1.9 2,589,729 - +2.5 2,590,111 + Psyr_2228 0.47 -2.4 2,590,112 - Psyr_2228 0.47 +0.5 2,590,112 - Psyr_2228 0.47 +3.0 2,590,112 - Psyr_2228 0.47 -1.1 2,590,112 - Psyr_2228 0.47 +3.6 2,590,126 + Psyr_2228 0.49 +2.2 2,590,126 + Psyr_2228 0.49 -0.1 2,590,127 - Psyr_2228 0.49 +2.5 2,590,136 + Psyr_2228 0.50 -0.4 2,590,136 + Psyr_2228 0.50 +2.2 2,590,137 - Psyr_2228 0.50 -1.5 2,590,137 - Psyr_2228 0.50 -1.6 2,590,325 - Psyr_2228 0.76 -2.1 2,590,530 + +4.5 2,590,531 - +2.5 2,590,580 - +1.9 2,590,611 + +1.5 2,590,611 + -4.2 2,590,611 + -0.1 2,590,612 - +2.2 2,590,712 + -0.0 2,590,773 + Psyr_2229 0.10 +1.9 2,590,773 + Psyr_2229 0.10 +1.5 2,590,774 - Psyr_2229 0.10 -0.5 2,590,779 + Psyr_2229 0.11 +3.2 2,590,827 + Psyr_2229 0.13 +2.5 2,590,828 - Psyr_2229 0.13 +3.9 2,590,828 - Psyr_2229 0.13 +1.3 2,590,923 + Psyr_2229 0.18 -0.1 2,591,331 + Psyr_2229 0.37 +3.2 2,591,416 - Psyr_2229 0.41 +2.5 2,591,472 + Psyr_2229 0.44 +2.5 2,591,472 + Psyr_2229 0.44 -0.1 2,591,472 + Psyr_2229 0.44 +1.9 2,591,472 + Psyr_2229 0.44 -2.1 2,591,472 + Psyr_2229 0.44 -1.5 2,591,472 + Psyr_2229 0.44 -1.2 2,591,472 + Psyr_2229 0.44 +2.2 2,591,473 - Psyr_2229 0.44 -1.5 2,591,473 - Psyr_2229 0.44 +2.9 2,591,473 - Psyr_2229 0.44 -2.5 2,591,473 - Psyr_2229 0.44 -0.5 2,591,473 - Psyr_2229 0.44 -0.4 2,591,530 - Psyr_2229 0.46 +4.7 2,591,785 + Psyr_2229 0.58 -1.8 2,591,785 + Psyr_2229 0.58 +1.5 2,591,786 - Psyr_2229 0.58 -1.7 2,591,984 + Psyr_2229 0.68 +3.9 2,591,984 + Psyr_2229 0.68 +0.9 2,591,984 + Psyr_2229 0.68 +2.5 2,591,985 - Psyr_2229 0.68 -1.5 2,591,985 - Psyr_2229 0.68 +0.9 2,591,985 - Psyr_2229 0.68 -1.8 2,591,985 - Psyr_2229 0.68 -0.0 2,592,124 + Psyr_2229 0.74 +1.9 2,592,223 + Psyr_2229 0.79 +1.5 2,592,224 - Psyr_2229 0.79 +2.5 2,592,233 - Psyr_2229 0.80 -2.3 2,592,288 + Psyr_2229 0.82 +1.9 2,592,288 + Psyr_2229 0.82 -1.3 2,592,288 + Psyr_2229 0.82 -0.1 2,592,289 - Psyr_2229 0.82 -1.2 2,592,328 - Psyr_2229 0.84 -1.9 2,592,349 + Psyr_2229 0.85 +1.5 2,592,741 + +2.7 2,592,741 + +1.9 2,592,742 - +2.5 2,592,754 + +2.9 2,592,793 + +2.9 2,592,794 - -1.6 2,592,947 + +3.5 2,592,948 - +1.9 2,592,988 + Psyr_2230 0.10 -1.9 2,593,021 - Psyr_2230 0.13 +2.5 2,593,271 + Psyr_2230 0.32 -2.7 2,593,271 + Psyr_2230 0.32 +1.9 2,593,271 + Psyr_2230 0.32 -1.0 2,593,271 + Psyr_2230 0.32 -0.3 2,593,271 + Psyr_2230 0.32 +3.5 2,593,271 + Psyr_2230 0.32 +0.9 2,593,271 + Psyr_2230 0.32 +3.0 2,593,272 - Psyr_2230 0.32 -3.1 2,593,272 - Psyr_2230 0.32 -1.9 2,593,272 - Psyr_2230 0.32 -2.6 2,593,478 + Psyr_2230 0.48 +3.6 2,593,479 - Psyr_2230 0.48 +1.3 2,593,495 - Psyr_2230 0.49 -1.8 2,593,510 + Psyr_2230 0.50 -0.1
Or see this region's nucleotide sequence