Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2215 and Psyr_2216 are separated by 64 nucleotides Psyr_2216 and Psyr_2217 are separated by 87 nucleotides Psyr_2217 and Psyr_2218 are separated by 123 nucleotides Psyr_2218 and Psyr_2219 are separated by 45 nucleotides
Psyr_2215: Psyr_2215 - CheW-like protein, at 2,575,434 to 2,576,912
_2215
Psyr_2216: Psyr_2216 - conserved hypothetical protein, at 2,576,977 to 2,577,102
_2216
Psyr_2217: Psyr_2217 - conserved hypothetical protein, at 2,577,190 to 2,577,588
_2217
Psyr_2218: Psyr_2218 - Beta-lactamase-like protein, at 2,577,712 to 2,578,587
_2218
Psyr_2219: Psyr_2219 - transcriptional regulator, LysR family, at 2,578,633 to 2,579,586
_2219
Position (kb)
2577
2578
2579 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2576.945 kb on + strand at 2576.946 kb on - strand at 2577.625 kb on + strand at 2577.625 kb on + strand at 2577.625 kb on + strand at 2577.625 kb on + strand at 2577.626 kb on - strand at 2577.626 kb on - strand at 2577.637 kb on + strand at 2578.075 kb on + strand, within Psyr_2218 at 2578.076 kb on - strand, within Psyr_2218 at 2578.076 kb on - strand, within Psyr_2218 at 2578.079 kb on + strand, within Psyr_2218 at 2578.079 kb on + strand, within Psyr_2218 at 2578.079 kb on + strand, within Psyr_2218 at 2578.079 kb on + strand, within Psyr_2218 at 2578.159 kb on + strand, within Psyr_2218 at 2578.160 kb on - strand at 2578.160 kb on - strand, within Psyr_2218 at 2578.160 kb on - strand, within Psyr_2218 at 2578.319 kb on + strand, within Psyr_2218 at 2578.319 kb on + strand, within Psyr_2218 at 2578.319 kb on + strand, within Psyr_2218 at 2578.319 kb on + strand, within Psyr_2218 at 2578.319 kb on + strand, within Psyr_2218 at 2578.319 kb on + strand, within Psyr_2218 at 2578.319 kb on + strand, within Psyr_2218 at 2578.319 kb on + strand, within Psyr_2218 at 2578.320 kb on - strand, within Psyr_2218 at 2578.320 kb on - strand, within Psyr_2218 at 2578.320 kb on - strand, within Psyr_2218 at 2578.322 kb on + strand, within Psyr_2218 at 2578.322 kb on + strand, within Psyr_2218 at 2578.346 kb on + strand, within Psyr_2218 at 2578.347 kb on - strand, within Psyr_2218 at 2578.347 kb on - strand, within Psyr_2218 at 2578.347 kb on - strand, within Psyr_2218 at 2578.347 kb on - strand, within Psyr_2218 at 2578.499 kb on + strand, within Psyr_2218 at 2578.500 kb on - strand at 2578.500 kb on - strand at 2578.629 kb on + strand at 2578.629 kb on + strand at 2578.630 kb on - strand at 2578.682 kb on - strand at 2578.683 kb on + strand at 2578.777 kb on - strand at 2578.777 kb on - strand, within Psyr_2219 at 2579.153 kb on + strand, within Psyr_2219 at 2579.153 kb on + strand, within Psyr_2219 at 2579.153 kb on + strand, within Psyr_2219 at 2579.153 kb on + strand, within Psyr_2219 at 2579.153 kb on + strand, within Psyr_2219 at 2579.154 kb on - strand, within Psyr_2219 at 2579.154 kb on - strand, within Psyr_2219 at 2579.154 kb on - strand, within Psyr_2219 at 2579.154 kb on - strand, within Psyr_2219 at 2579.256 kb on + strand, within Psyr_2219 at 2579.257 kb on - strand, within Psyr_2219 at 2579.269 kb on + strand, within Psyr_2219 at 2579.270 kb on - strand, within Psyr_2219 at 2579.504 kb on - strand at 2579.504 kb on - strand at 2579.504 kb on - strand at 2579.584 kb on + strand at 2579.585 kb on - strand at 2579.585 kb on - strand at 2579.585 kb on - strand at 2579.585 kb on - strand at 2579.585 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 2,576,945 + +1.4 2,576,946 - +2.7 2,577,625 + +2.2 2,577,625 + -2.1 2,577,625 + +3.0 2,577,625 + +3.5 2,577,626 - -2.5 2,577,626 - -0.8 2,577,637 + -2.4 2,578,075 + Psyr_2218 0.41 -0.1 2,578,076 - Psyr_2218 0.42 +3.5 2,578,076 - Psyr_2218 0.42 +1.3 2,578,079 + Psyr_2218 0.42 -0.5 2,578,079 + Psyr_2218 0.42 +0.7 2,578,079 + Psyr_2218 0.42 +1.5 2,578,079 + Psyr_2218 0.42 -0.3 2,578,159 + Psyr_2218 0.51 +2.5 2,578,160 - -0.9 2,578,160 - Psyr_2218 0.51 +2.9 2,578,160 - Psyr_2218 0.51 +3.6 2,578,319 + Psyr_2218 0.69 +3.3 2,578,319 + Psyr_2218 0.69 +3.5 2,578,319 + Psyr_2218 0.69 +0.9 2,578,319 + Psyr_2218 0.69 +1.5 2,578,319 + Psyr_2218 0.69 +3.2 2,578,319 + Psyr_2218 0.69 -0.7 2,578,319 + Psyr_2218 0.69 +3.0 2,578,319 + Psyr_2218 0.69 +2.2 2,578,320 - Psyr_2218 0.69 +0.5 2,578,320 - Psyr_2218 0.69 +0.8 2,578,320 - Psyr_2218 0.69 +2.9 2,578,322 + Psyr_2218 0.70 +2.7 2,578,322 + Psyr_2218 0.70 +2.7 2,578,346 + Psyr_2218 0.72 +2.2 2,578,347 - Psyr_2218 0.72 +0.9 2,578,347 - Psyr_2218 0.72 -2.1 2,578,347 - Psyr_2218 0.72 +3.5 2,578,347 - Psyr_2218 0.72 -3.0 2,578,499 + Psyr_2218 0.90 +2.7 2,578,500 - +1.5 2,578,500 - +0.1 2,578,629 + +0.9 2,578,629 + +0.9 2,578,630 - -1.9 2,578,682 - +0.1 2,578,683 + +2.2 2,578,777 - -0.1 2,578,777 - Psyr_2219 0.15 -0.1 2,579,153 + Psyr_2219 0.55 +2.2 2,579,153 + Psyr_2219 0.55 -1.5 2,579,153 + Psyr_2219 0.55 -0.4 2,579,153 + Psyr_2219 0.55 +2.9 2,579,153 + Psyr_2219 0.55 -2.5 2,579,154 - Psyr_2219 0.55 +2.2 2,579,154 - Psyr_2219 0.55 +3.0 2,579,154 - Psyr_2219 0.55 +1.5 2,579,154 - Psyr_2219 0.55 +4.1 2,579,256 + Psyr_2219 0.65 -1.2 2,579,257 - Psyr_2219 0.65 -1.4 2,579,269 + Psyr_2219 0.67 -0.8 2,579,270 - Psyr_2219 0.67 -0.3 2,579,504 - +1.5 2,579,504 - +0.2 2,579,504 - +0.9 2,579,584 + -0.1 2,579,585 - -0.5 2,579,585 - -0.4 2,579,585 - +3.7 2,579,585 - -2.7 2,579,585 - -0.1
Or see this region's nucleotide sequence